Gene Details:
- Gene ID: Glyma.11G238800
- Gene Symbol: GmMIPS1
- Gene Name: myo-inositol-3-phosphate synthase1
- Genome: Wm82.a4.v1
- Species: Glycine max
Functional Descriptions:
- RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content.
- Merrill lines were produced using interfering RNA (RNAi) construct in order to silence the myo-inositol-1-phosphate (GmMIPS1) gene. We have observed an absence of seed development in lines in which the presence of GmMIPS1 transcripts was not detected.
- In addition, a drastic reduction of phytate (InsP6) content was achieved in transgenic lines (up to 94.5%). Our results demonstrated an important correlation between GmMIPS1 gene expression and seed development.
- RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content.
- Experiments have been carried out to evaluate utilization of the GmMIPS1 silencing strategy to inhibit seed development in fructiferous plants with the potential to develop seedless fruits.
Function-related keywords:
Literature:
- RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. DOI: 10.1007/s00425-005-0201-0 ; PMID: 16395584
- RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content. DOI: 10.1007/s00425-005-0201-0 ; PMID: 16395584
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Glyma.11G238800.1
GCCTTTGCAAATGGCCAAGGCAAGCGCTGGGCTTTGAAATGGAGCATTTCAGAATCATCAATGATGTAACCCGCAACCCACTCAAGTTGTGACACGTGTATAAGCATTCTATCTCGTTCCTCTATATATAGGACCTCCTTCACCCAACACAAGTGTGTGTAGTATAGGATTCTCTTCTTTATTCCTTTTGTAATTTCATTCATTCTTAATCTTTGTGAAAAATAATGTTCATCGAGAATTTTAAGGTTGAGTGTCCTAATGTGAAGTACACCGAGACTGAGATTCAGTCCGTGTACAACTACGAAACCACCGAACTTGTTCACGAGAACAGGAATGGCACCTATCAGTGGATTGTCAAACCCAAATCTGTCAAATACGAATTTAAAACCAACATCCATGTTCCTAAATTAGGGGTAATGCTTGTGGGTTGGGGTGGAAACAACGGCTCAACCCTCACCGGTGGTGTTATTGCTAACCGAGAGGGCATTTCATGGGCTACAAAGGACAAGATTCAACAAGCCAATTACTTTGGCTCCCTCACCCAAGCCTCAGCTATCCGAGTTGGGTCCTTCCAGGGAGAGGAAATCTATGCCCCATTCAAGAGCCTGCTTCCAATGGTTAACCCTGACGACATTGTGTTTGGGGGATGGGATATCAGCAACATGAACCTGGCTGATGCCATGGCCAGGGCAAAGGTGTTTGACATCGATTTGCAGAAGCAGTTGAGGCCTTACATGGAATCCATGCTTCCACTCCCCGGAATCTATGACCCGGATTTCATTGCTGCCAACCAAGAGGAGCGTGCCAACAACGTCATCAAGGGCACAAAGCAAGAGCAAGTTCAACAAATCATCAAAGACATCAAGGCGTTTAAGGAAGCCACCAAAGTGGACAAGGTGGTTGTACTGTGGACTGCCAACACAGAGAGGTACAGTAATTTGGTTGTGGGCCTTAATGACACCATGGAGAATCTCTTGGCTGCTGTGGACAGAAATGAGGCTGAGATTTCTCCTTCCACCTTGTATGCCATTGCTTGTGTTATGGAAAATGTTCCTTTCATTAATGGAAGCCCTCAGAACACTTTTGTACCAGGGCTGATTGATCTTGCCATCGCGAGGAACACTTTGATTGGTGGAGATGACTTCAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTTGATTTCCTTGTGGGGGCTGGTATCAAGCCAACATCTATAGTCAGTTACAACCATCTGGGAAACAATGATGGTATGAATCTTTCGGCTCCACAAACTTTCCGTTCCAAGGAAATCTCCAAGAGCAACGTTGTTGATGATATGGTCAACAGCAATGCCATCCTCTATGAGCCTGGTGAACATCCAGACCATGTTGTTGTTATTAAGTATGTGCCTTACGTAGGGGACAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGTGGAAAGAGCACCATTGTTTTGCACAACACATGCGAGGATTCCCTCTTAGCTGCTCCTATTATCTTGGACTTGGTCCTTCTTGCTGAGCTCAGCACTAGAATCGAGTTTAAAGCTGAAAATGAGGGAAAATTCCACTCATTCCACCCAGTTGCTACCATCCTCAGCTACCTCACCAAGGCTCCTCTGGTTCCACCGGGTACACCAGTGGTGAATGCATTGTCAAAGCAGCGTGCAATGCTGGAAAACATAATGAGGGCTTGTGTTGGATTGGCCCCAGAGAATAACATGATTCTCGAGTACAAGTGAAGCATGGGACCGAAGAATAATATAGTTGGGGTAGCCTAGCTGAATGTTTTATGTTAATAATATGTTTGCTTATAATTTTGCAAGTGTAATTGAATGCATCAGCTTCATTAATGCTTTAGAGCGGGGCATATTCTGTTTACTAGGAACATGAATGAATGTAGTATAATTTTGTGTACTTTATCCTC
CDS Sequence
- >Glyma.11G238800.1
ATGTTCATCGAGAATTTTAAGGTTGAGTGTCCTAATGTGAAGTACACCGAGACTGAGATTCAGTCCGTGTACAACTACGAAACCACCGAACTTGTTCACGAGAACAGGAATGGCACCTATCAGTGGATTGTCAAACCCAAATCTGTCAAATACGAATTTAAAACCAACATCCATGTTCCTAAATTAGGGGTAATGCTTGTGGGTTGGGGTGGAAACAACGGCTCAACCCTCACCGGTGGTGTTATTGCTAACCGAGAGGGCATTTCATGGGCTACAAAGGACAAGATTCAACAAGCCAATTACTTTGGCTCCCTCACCCAAGCCTCAGCTATCCGAGTTGGGTCCTTCCAGGGAGAGGAAATCTATGCCCCATTCAAGAGCCTGCTTCCAATGGTTAACCCTGACGACATTGTGTTTGGGGGATGGGATATCAGCAACATGAACCTGGCTGATGCCATGGCCAGGGCAAAGGTGTTTGACATCGATTTGCAGAAGCAGTTGAGGCCTTACATGGAATCCATGCTTCCACTCCCCGGAATCTATGACCCGGATTTCATTGCTGCCAACCAAGAGGAGCGTGCCAACAACGTCATCAAGGGCACAAAGCAAGAGCAAGTTCAACAAATCATCAAAGACATCAAGGCGTTTAAGGAAGCCACCAAAGTGGACAAGGTGGTTGTACTGTGGACTGCCAACACAGAGAGGTACAGTAATTTGGTTGTGGGCCTTAATGACACCATGGAGAATCTCTTGGCTGCTGTGGACAGAAATGAGGCTGAGATTTCTCCTTCCACCTTGTATGCCATTGCTTGTGTTATGGAAAATGTTCCTTTCATTAATGGAAGCCCTCAGAACACTTTTGTACCAGGGCTGATTGATCTTGCCATCGCGAGGAACACTTTGATTGGTGGAGATGACTTCAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTTGATTTCCTTGTGGGGGCTGGTATCAAGCCAACATCTATAGTCAGTTACAACCATCTGGGAAACAATGATGGTATGAATCTTTCGGCTCCACAAACTTTCCGTTCCAAGGAAATCTCCAAGAGCAACGTTGTTGATGATATGGTCAACAGCAATGCCATCCTCTATGAGCCTGGTGAACATCCAGACCATGTTGTTGTTATTAAGTATGTGCCTTACGTAGGGGACAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGTGGAAAGAGCACCATTGTTTTGCACAACACATGCGAGGATTCCCTCTTAGCTGCTCCTATTATCTTGGACTTGGTCCTTCTTGCTGAGCTCAGCACTAGAATCGAGTTTAAAGCTGAAAATGAGGGAAAATTCCACTCATTCCACCCAGTTGCTACCATCCTCAGCTACCTCACCAAGGCTCCTCTGGTTCCACCGGGTACACCAGTGGTGAATGCATTGTCAAAGCAGCGTGCAATGCTGGAAAACATAATGAGGGCTTGTGTTGGATTGGCCCCAGAGAATAACATGATTCTCGAGTACAAGTGA
Protein Sequence
- >Glyma.11G238800.1
MFIENFKVECPNVKYTETEIQSVYNYETTELVHENRNGTYQWIVKPKSVKYEFKTNIHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKIQQANYFGSLTQASAIRVGSFQGEEIYAPFKSLLPMVNPDDIVFGGWDISNMNLADAMARAKVFDIDLQKQLRPYMESMLPLPGIYDPDFIAANQEERANNVIKGTKQEQVQQIIKDIKAFKEATKVDKVVVLWTANTERYSNLVVGLNDTMENLLAAVDRNEAEISPSTLYAIACVMENVPFINGSPQNTFVPGLIDLAIARNTLIGGDDFKSGQTKMKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKSNVVDDMVNSNAILYEPGEHPDHVVVIKYVPYVGDSKRAMDEYTSEIFMGGKSTIVLHNTCEDSLLAAPIILDLVLLAELSTRIEFKAENEGKFHSFHPVATILSYLTKAPLVPPGTPVVNALSKQRAMLENIMRACVGLAPENNMILEYK*