Gene Details:

  • Gene ID: Glyma.11G238800
  • Gene Symbol: GmMIPS1
  • Gene Name: myo-inositol-3-phosphate synthase1
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content.
  • Merrill lines were produced using interfering RNA (RNAi) construct in order to silence the myo-inositol-1-phosphate (GmMIPS1) gene. We have observed an absence of seed development in lines in which the presence of GmMIPS1 transcripts was not detected.
  • In addition, a drastic reduction of phytate (InsP6) content was achieved in transgenic lines (up to 94.5%). Our results demonstrated an important correlation between GmMIPS1 gene expression and seed development.
  • RNAi-mediated silencing of the myo-inositol-1-phosphate synthase gene (GmMIPS1) in transgenic soybean inhibited seed development and reduced phytate content.
  • Experiments have been carried out to evaluate utilization of the GmMIPS1 silencing strategy to inhibit seed development in fructiferous plants with the potential to develop seedless fruits.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.11G238800.1
    GCCTTTGCAAATGGCCAAGGCAAGCGCTGGGCTTTGAAATGGAGCATTTCAGAATCATCAATGATGTAACCCGCAACCCACTCAAGTTGTGACACGTGTATAAGCATTCTATCTCGTTCCTCTATATATAGGACCTCCTTCACCCAACACAAGTGTGTGTAGTATAGGATTCTCTTCTTTATTCCTTTTGTAATTTCATTCATTCTTAATCTTTGTGAAAAATAATGTTCATCGAGAATTTTAAGGTTGAGTGTCCTAATGTGAAGTACACCGAGACTGAGATTCAGTCCGTGTACAACTACGAAACCACCGAACTTGTTCACGAGAACAGGAATGGCACCTATCAGTGGATTGTCAAACCCAAATCTGTCAAATACGAATTTAAAACCAACATCCATGTTCCTAAATTAGGGGTAATGCTTGTGGGTTGGGGTGGAAACAACGGCTCAACCCTCACCGGTGGTGTTATTGCTAACCGAGAGGGCATTTCATGGGCTACAAAGGACAAGATTCAACAAGCCAATTACTTTGGCTCCCTCACCCAAGCCTCAGCTATCCGAGTTGGGTCCTTCCAGGGAGAGGAAATCTATGCCCCATTCAAGAGCCTGCTTCCAATGGTTAACCCTGACGACATTGTGTTTGGGGGATGGGATATCAGCAACATGAACCTGGCTGATGCCATGGCCAGGGCAAAGGTGTTTGACATCGATTTGCAGAAGCAGTTGAGGCCTTACATGGAATCCATGCTTCCACTCCCCGGAATCTATGACCCGGATTTCATTGCTGCCAACCAAGAGGAGCGTGCCAACAACGTCATCAAGGGCACAAAGCAAGAGCAAGTTCAACAAATCATCAAAGACATCAAGGCGTTTAAGGAAGCCACCAAAGTGGACAAGGTGGTTGTACTGTGGACTGCCAACACAGAGAGGTACAGTAATTTGGTTGTGGGCCTTAATGACACCATGGAGAATCTCTTGGCTGCTGTGGACAGAAATGAGGCTGAGATTTCTCCTTCCACCTTGTATGCCATTGCTTGTGTTATGGAAAATGTTCCTTTCATTAATGGAAGCCCTCAGAACACTTTTGTACCAGGGCTGATTGATCTTGCCATCGCGAGGAACACTTTGATTGGTGGAGATGACTTCAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTTGATTTCCTTGTGGGGGCTGGTATCAAGCCAACATCTATAGTCAGTTACAACCATCTGGGAAACAATGATGGTATGAATCTTTCGGCTCCACAAACTTTCCGTTCCAAGGAAATCTCCAAGAGCAACGTTGTTGATGATATGGTCAACAGCAATGCCATCCTCTATGAGCCTGGTGAACATCCAGACCATGTTGTTGTTATTAAGTATGTGCCTTACGTAGGGGACAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGTGGAAAGAGCACCATTGTTTTGCACAACACATGCGAGGATTCCCTCTTAGCTGCTCCTATTATCTTGGACTTGGTCCTTCTTGCTGAGCTCAGCACTAGAATCGAGTTTAAAGCTGAAAATGAGGGAAAATTCCACTCATTCCACCCAGTTGCTACCATCCTCAGCTACCTCACCAAGGCTCCTCTGGTTCCACCGGGTACACCAGTGGTGAATGCATTGTCAAAGCAGCGTGCAATGCTGGAAAACATAATGAGGGCTTGTGTTGGATTGGCCCCAGAGAATAACATGATTCTCGAGTACAAGTGAAGCATGGGACCGAAGAATAATATAGTTGGGGTAGCCTAGCTGAATGTTTTATGTTAATAATATGTTTGCTTATAATTTTGCAAGTGTAATTGAATGCATCAGCTTCATTAATGCTTTAGAGCGGGGCATATTCTGTTTACTAGGAACATGAATGAATGTAGTATAATTTTGTGTACTTTATCCTC
CDS Sequence
  • >Glyma.11G238800.1
    ATGTTCATCGAGAATTTTAAGGTTGAGTGTCCTAATGTGAAGTACACCGAGACTGAGATTCAGTCCGTGTACAACTACGAAACCACCGAACTTGTTCACGAGAACAGGAATGGCACCTATCAGTGGATTGTCAAACCCAAATCTGTCAAATACGAATTTAAAACCAACATCCATGTTCCTAAATTAGGGGTAATGCTTGTGGGTTGGGGTGGAAACAACGGCTCAACCCTCACCGGTGGTGTTATTGCTAACCGAGAGGGCATTTCATGGGCTACAAAGGACAAGATTCAACAAGCCAATTACTTTGGCTCCCTCACCCAAGCCTCAGCTATCCGAGTTGGGTCCTTCCAGGGAGAGGAAATCTATGCCCCATTCAAGAGCCTGCTTCCAATGGTTAACCCTGACGACATTGTGTTTGGGGGATGGGATATCAGCAACATGAACCTGGCTGATGCCATGGCCAGGGCAAAGGTGTTTGACATCGATTTGCAGAAGCAGTTGAGGCCTTACATGGAATCCATGCTTCCACTCCCCGGAATCTATGACCCGGATTTCATTGCTGCCAACCAAGAGGAGCGTGCCAACAACGTCATCAAGGGCACAAAGCAAGAGCAAGTTCAACAAATCATCAAAGACATCAAGGCGTTTAAGGAAGCCACCAAAGTGGACAAGGTGGTTGTACTGTGGACTGCCAACACAGAGAGGTACAGTAATTTGGTTGTGGGCCTTAATGACACCATGGAGAATCTCTTGGCTGCTGTGGACAGAAATGAGGCTGAGATTTCTCCTTCCACCTTGTATGCCATTGCTTGTGTTATGGAAAATGTTCCTTTCATTAATGGAAGCCCTCAGAACACTTTTGTACCAGGGCTGATTGATCTTGCCATCGCGAGGAACACTTTGATTGGTGGAGATGACTTCAAGAGTGGTCAGACCAAAATGAAATCTGTGTTGGTTGATTTCCTTGTGGGGGCTGGTATCAAGCCAACATCTATAGTCAGTTACAACCATCTGGGAAACAATGATGGTATGAATCTTTCGGCTCCACAAACTTTCCGTTCCAAGGAAATCTCCAAGAGCAACGTTGTTGATGATATGGTCAACAGCAATGCCATCCTCTATGAGCCTGGTGAACATCCAGACCATGTTGTTGTTATTAAGTATGTGCCTTACGTAGGGGACAGCAAGAGAGCCATGGATGAGTACACTTCAGAGATATTCATGGGTGGAAAGAGCACCATTGTTTTGCACAACACATGCGAGGATTCCCTCTTAGCTGCTCCTATTATCTTGGACTTGGTCCTTCTTGCTGAGCTCAGCACTAGAATCGAGTTTAAAGCTGAAAATGAGGGAAAATTCCACTCATTCCACCCAGTTGCTACCATCCTCAGCTACCTCACCAAGGCTCCTCTGGTTCCACCGGGTACACCAGTGGTGAATGCATTGTCAAAGCAGCGTGCAATGCTGGAAAACATAATGAGGGCTTGTGTTGGATTGGCCCCAGAGAATAACATGATTCTCGAGTACAAGTGA
Protein Sequence
  • >Glyma.11G238800.1
    MFIENFKVECPNVKYTETEIQSVYNYETTELVHENRNGTYQWIVKPKSVKYEFKTNIHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKIQQANYFGSLTQASAIRVGSFQGEEIYAPFKSLLPMVNPDDIVFGGWDISNMNLADAMARAKVFDIDLQKQLRPYMESMLPLPGIYDPDFIAANQEERANNVIKGTKQEQVQQIIKDIKAFKEATKVDKVVVLWTANTERYSNLVVGLNDTMENLLAAVDRNEAEISPSTLYAIACVMENVPFINGSPQNTFVPGLIDLAIARNTLIGGDDFKSGQTKMKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKSNVVDDMVNSNAILYEPGEHPDHVVVIKYVPYVGDSKRAMDEYTSEIFMGGKSTIVLHNTCEDSLLAAPIILDLVLLAELSTRIEFKAENEGKFHSFHPVATILSYLTKAPLVPPGTPVVNALSKQRAMLENIMRACVGLAPENNMILEYK*