Gene Details:

Functional Descriptions:

  • Overexpression of GmAAP6a enhances tolerance to low nitrogen and improves seed nitrogen status by optimizing amino acid partitioning in soybean.
  • Amino acid permeases (AAPs) play essential roles in amino acid transport between plant cells and subsequent phloem or seed loading. In this study, a soybean AAP gene, annotated as GmAAP6a, was cloned and demonstrated to be significantly induced by nitrogen starvation.
  • Growth and transport studies using toxic amino acid analogs or single amino acids as a sole nitrogen source suggest that GmAAP6a can selectively absorb and transport neutral and acidic amino acids.
  • Overexpression of GmAAP6a in Arabidopsis and soybean resulted in elevated tolerance to nitrogen limitation.
  • Our results imply that overexpression of GmAAP6a enhances nitrogen stress tolerance and source-to-sink transport and improves seed quality in soybean.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Glyma.17G192000.1
    ATACCATGCCTCTTGGTCCTTTCTCTAATCCAAATATCTCTCCGTTAATGCTCTCACCACAAACTTCAATAGACTAGCCAAAAGCTAACACCAACTATAGGCCAAATCACCAATAAATATTATTTCCCGTTCTTTTGGCGTGTTGGCCACCCACTGAACACATTTGCACTCACACATAGAAGCAGAGAGAGGCCATTGGTTGGTTTCAGAGATGGGAAGCATGCACATAGAAACCCCAGAAACTTTTGCTGATGGTAGCAAAAACTTCGATGATGATGGACGAGCTAAAAGAACTGGGACTTGGATTACTGCAAGTGCTCATATCATAACGGCTGTGATTGGTTCTGGAGTGCTATCTCTTGCATGGGCAATTGCACAGATGGGTTGGGTAGCTGGTCCTGCAGTGCTCTTTGTCTTCTCTTTGATAACATACTTCACCTCCACTCTTCTCGCTGACTGTTACCGTTCACCTGACCCTGTACATGGCAAGCGAAACTACACATATTCTGAGGTTGTCAAAGCCAACCTAGGAGGAAGAAAATTTCAGCTGTGTGGATTGGCTCAGTATATAAATCTTGTTGGTGTAACAATCGGCTACACTATAACTGCATCACTTAGTATGGGGGCGGTGAAGAAGTCGAACTGTTTACACAAACATGGCCATCAAGACGAGTGCAAAGTTAAGGACAACGCTTTTATGATTGCTTTTGCCTGCATCCAAATTCTTCTAAGCCAAATACCAAACTTCCATAAGCTCTCTTGGCTCTCTATCGTAGCAGCTGTTATGTCTTTTGCTTATTCTTCCATTGGGCTTGGCCTTTCCATAGCCAAAATCATAGGTGGGGGACACGTGCGAACAACCTTAACAGGGGTGGAAGTTTCGGGAACGGAAAAGGTTTGGAAAATGTTTCAAGCTATCGGTGACATTGCCTTTGCTTATGCTTTTTCTAATGTCCTAATTGAGATCCAGGACACACTGAAATCAAGCCCACCTGAGAACAAAGTCATGAAGAGAGCAAGTTTGATTGGCATTATGACTACAACATTGTTTTATGTACTATGTGGGTGCTTAGGCTATGCTGCATTTGGAAATGATGCACCATCTAATTTCCTCACAGGATTCGGCTTCTATGAGCCCTTTTGGCTAATAGACTTTGCCAATGTCTGCATAGCAGTGCACTTAGTTGGGGCATACCAGGTCTTTGTCCAACCTATATTTGGGTTTGTGGAAAAGTGGAGCAAAGAAAATTGGACAGAAAGCCAATTTATAAATGGCGAGCATACTTTGAACATTCCTCTATGTGGAAGCTACAATGTGAACTTCTTTAGGGTAGTGTGGAGGACTGCATATGTGATTATCACTGCCGTTGTAGCTATGTTACTCCCATTCTTCAATGACTTTTTGGCCCTTATTGGTGCACTCTCTTTCTGGCCATTGACGGTTTACTTCCCTATAGAGATGTACATTAAGAAGTCAAATATGCAAAGATTTTCCTTCACCTGGACTTGGCTCAAGATATTGAGTTGGGTTTGCTTGATCATTTCTATTATCTCACTTGTGGGTTCCATCCAAGGCCTTTCGGTTAGTATCAAGAAATACAAGCCCTTCCAAGCAGAACAATAGAACTTTCATACTTAGTATTCCAACTTGAGCCAAGAGTTTCTATTGAGGGCGACAAAATTTGTATTACTATGTTTTGAAATAGTTGTTATATTTTCAATTCTCGAAATCGAGACAACTTGTTATACTAAAGTAATATAATAAAATGTTTAGTTTGCTATGTTACATGGTTTTCTTCTTGGTAGCTGTTTTTTTTTCTTTTTTGTTAGGTTATGTTTAGATTGATGGAAGAGGATGGAATAAAATGAAT
CDS Sequence
  • >Glyma.17G192000.1
    ATGGGAAGCATGCACATAGAAACCCCAGAAACTTTTGCTGATGGTAGCAAAAACTTCGATGATGATGGACGAGCTAAAAGAACTGGGACTTGGATTACTGCAAGTGCTCATATCATAACGGCTGTGATTGGTTCTGGAGTGCTATCTCTTGCATGGGCAATTGCACAGATGGGTTGGGTAGCTGGTCCTGCAGTGCTCTTTGTCTTCTCTTTGATAACATACTTCACCTCCACTCTTCTCGCTGACTGTTACCGTTCACCTGACCCTGTACATGGCAAGCGAAACTACACATATTCTGAGGTTGTCAAAGCCAACCTAGGAGGAAGAAAATTTCAGCTGTGTGGATTGGCTCAGTATATAAATCTTGTTGGTGTAACAATCGGCTACACTATAACTGCATCACTTAGTATGGGGGCGGTGAAGAAGTCGAACTGTTTACACAAACATGGCCATCAAGACGAGTGCAAAGTTAAGGACAACGCTTTTATGATTGCTTTTGCCTGCATCCAAATTCTTCTAAGCCAAATACCAAACTTCCATAAGCTCTCTTGGCTCTCTATCGTAGCAGCTGTTATGTCTTTTGCTTATTCTTCCATTGGGCTTGGCCTTTCCATAGCCAAAATCATAGGTGGGGGACACGTGCGAACAACCTTAACAGGGGTGGAAGTTTCGGGAACGGAAAAGGTTTGGAAAATGTTTCAAGCTATCGGTGACATTGCCTTTGCTTATGCTTTTTCTAATGTCCTAATTGAGATCCAGGACACACTGAAATCAAGCCCACCTGAGAACAAAGTCATGAAGAGAGCAAGTTTGATTGGCATTATGACTACAACATTGTTTTATGTACTATGTGGGTGCTTAGGCTATGCTGCATTTGGAAATGATGCACCATCTAATTTCCTCACAGGATTCGGCTTCTATGAGCCCTTTTGGCTAATAGACTTTGCCAATGTCTGCATAGCAGTGCACTTAGTTGGGGCATACCAGGTCTTTGTCCAACCTATATTTGGGTTTGTGGAAAAGTGGAGCAAAGAAAATTGGACAGAAAGCCAATTTATAAATGGCGAGCATACTTTGAACATTCCTCTATGTGGAAGCTACAATGTGAACTTCTTTAGGGTAGTGTGGAGGACTGCATATGTGATTATCACTGCCGTTGTAGCTATGTTACTCCCATTCTTCAATGACTTTTTGGCCCTTATTGGTGCACTCTCTTTCTGGCCATTGACGGTTTACTTCCCTATAGAGATGTACATTAAGAAGTCAAATATGCAAAGATTTTCCTTCACCTGGACTTGGCTCAAGATATTGAGTTGGGTTTGCTTGATCATTTCTATTATCTCACTTGTGGGTTCCATCCAAGGCCTTTCGGTTAGTATCAAGAAATACAAGCCCTTCCAAGCAGAACAATAG
Protein Sequence
  • >Glyma.17G192000.1
    MGSMHIETPETFADGSKNFDDDGRAKRTGTWITASAHIITAVIGSGVLSLAWAIAQMGWVAGPAVLFVFSLITYFTSTLLADCYRSPDPVHGKRNYTYSEVVKANLGGRKFQLCGLAQYINLVGVTIGYTITASLSMGAVKKSNCLHKHGHQDECKVKDNAFMIAFACIQILLSQIPNFHKLSWLSIVAAVMSFAYSSIGLGLSIAKIIGGGHVRTTLTGVEVSGTEKVWKMFQAIGDIAFAYAFSNVLIEIQDTLKSSPPENKVMKRASLIGIMTTTLFYVLCGCLGYAAFGNDAPSNFLTGFGFYEPFWLIDFANVCIAVHLVGAYQVFVQPIFGFVEKWSKENWTESQFINGEHTLNIPLCGSYNVNFFRVVWRTAYVIITAVVAMLLPFFNDFLALIGALSFWPLTVYFPIEMYIKKSNMQRFSFTWTWLKILSWVCLIISIISLVGSIQGLSVSIKKYKPFQAEQ*