Gene Details:

  • Gene ID: Glyma.10g278000
  • Gene Symbol: FAD2-1A
  • Gene Name: omega-6 fatty acid desaturase
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Mutant alleles of FAD2-1A and FAD2-1Bcombine to produce soybeans with the high oleic acid seed oil trait.
  • The objective of this work was to create the high oleic acid trait in soybeans by identifying and combining mutations in two delta-twelve fatty acid desaturase genes, FAD2-1A and FAD2-1B.
  • The mutant FAD2-1B alleles were associated with an increase in oleic acid levels, although the FAD2-1B mutant alleles alone were not capable of producing a high oleic acid phenotype.
  • When existing FAD2-1A mutations were combined with the novel mutant FAD2-1B alleles, a high oleic acid phenotype was recovered only for those lines which were homozygous for both of the mutant alleles.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.10G278000.1
    CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC
  • >Glyma.10G278000.2
    CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC
  • >Glyma.10G278000.3
    CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC
CDS Sequence
  • >Glyma.10G278000.1
    ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA
  • >Glyma.10G278000.2
    ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA
  • >Glyma.10G278000.3
    ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA
Protein Sequence
  • >Glyma.10G278000.1
    MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY*
  • >Glyma.10G278000.2
    MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY*
  • >Glyma.10G278000.3
    MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY*