Gene Details:

Functional Descriptions:

  • GmCHLI1, a gene encoding the I subunit of magnesium (Mg)-chelatase, which functions in hexamers as responsible for the semi-dominant etiolation phenotype in soybean.
  • We performed map-based cloning and found that a CHLI homolog in soybean, GmCHLI1, is responsible for the etiolation phenotype.
  • These results indicate that the GmCHLI1 and GmCHLI2 missense mutations not only affect interaction capacity but also influence the transcript abundance of Mg-chelatase subunits and subsequently block chlorophyll biosynthesis.
  • These results indicate that all four GmCHLIs can function as the CHLI subunit but that their functions could be dominant negatively affected by non-synonymous mutation of either copy of GmCHLI1 and GmCHLI2, further suggesting that the different copies might interacted in nature.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.13G232500.1
    ACAGAGAGAGAAGAATGAAATCTATCTATCTTCTTATCCGAAGTCTGGGAGGCCAATAGGAAGCACGCCAGCTGCTACGAATGGTGAATAAAAGACAAAAGAAACAAACTGCTACATAGCATACAGTCTGTCTTCTCTTCTCTTCTCCGGTTATGGCGTCCGCCTTGGGCACTTCTTCAATTGCGGTTCTGCCTTCGCGCTACTTCTCTTCTTCTTCTTCCAAGCCTTCCATTCACACTCTCTCTCTAACTTCAGGGCAGAACTATGGGCGGAAGTTTTATGGAGGAATTGGAATCCATGGCATAAAGGGAAGGGCTCAGCTCTCGGTTACCAATGTTGCCACTGAAGTTAACTCTGTAGAACAGGCTCAGAGTATTGCTTCTAAAGAAAGCCAGAGGCCAGTATACCCATTTTCTGCCATAGTTGGACAAGATGAGATGAAGCTTTGTCTTCTCCTTAATGTGATTGATCCTAAGATTGGAGGTGTAATGATCATGGGGGATAGGGGCACAGGGAAATCTACAACGGTCAGGTCATTGGTTGATTTACTTCCCGAAATCAAGGTTGTTGCTGGTGACCCTTATAACTCAGACCCACAAGATCCAGAATTCATGGGTGTTGAAGTCAGAGAGCGTGTACTTCAAGGAGAGGAACTTTCTGTTGTCTTGACCAAAATTAACATGGTTGATTTGCCATTGGGAGCTACAGAAGATAGAGTGTGTGGAACGATTGACATTGAGAAAGCCCTGACTGAGGGTGTCAAGGCATTTGAGCCTGGACTACTGGCTAAAGCTAATAGGGGAATCTTATATGTTGATGAAGTTAATCTTTTGGATGATCACTTGGTGGATGTGTTGTTGGATTCTGCTGCATCAGGATGGAACACAGTAGAGAGAGAGGGAATTTCTATCTCGCATCCTGCACGGTTTATCCTAATTGGCTCAGGCAACCCCGAAGAAGGGGAGCTCCGGCCGCAGCTGCTAGATAGGTTTGGAATGCATGCTCAAGTTGGAACTGTTAGGGATGCTGAGCTCAGAGTGAAGATTGTGGAGGAGAGAGGTCGATTTGACAAAAATCCAAAGGAATTTCGAGATTCTTACAAAGCTGAGCAAGAGAAGCTCCAACAACAAATTACCTCAGCAAGGAGTGTTCTTTCTTCTGTTCAAATTGATCAAGATCTCAAGGTGAAAATCTCCAAGGTGTGTGCTGAGTTGAATGTGGATGGATTAAGAGGAGACATAGTAACAAATAGAGCTGCAAAAGCTCTGGCTGCTCTGAAGGGAAGAGACAACGTAAGTGCCGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACACCGTCTTAGAAAGGATCCCTTGGAGTCTATAGACTCAGGTTTACTTGTCACTGAGAAATTTTATGAGGTATTCAGCTGAGGATCATAGATGTAACACGGTTTGTATTCTGTGGTATTCCATGAGTATAATGCTACTTCATTATTTACATTTTACCTTTTCTGTGGAGATTTTCATTGTCGTTAAACATCAATAAGATTCATGAGTTCCTTCTACACAGGAAATT
CDS Sequence
  • >Glyma.13G232500.1
    ATGGCGTCCGCCTTGGGCACTTCTTCAATTGCGGTTCTGCCTTCGCGCTACTTCTCTTCTTCTTCTTCCAAGCCTTCCATTCACACTCTCTCTCTAACTTCAGGGCAGAACTATGGGCGGAAGTTTTATGGAGGAATTGGAATCCATGGCATAAAGGGAAGGGCTCAGCTCTCGGTTACCAATGTTGCCACTGAAGTTAACTCTGTAGAACAGGCTCAGAGTATTGCTTCTAAAGAAAGCCAGAGGCCAGTATACCCATTTTCTGCCATAGTTGGACAAGATGAGATGAAGCTTTGTCTTCTCCTTAATGTGATTGATCCTAAGATTGGAGGTGTAATGATCATGGGGGATAGGGGCACAGGGAAATCTACAACGGTCAGGTCATTGGTTGATTTACTTCCCGAAATCAAGGTTGTTGCTGGTGACCCTTATAACTCAGACCCACAAGATCCAGAATTCATGGGTGTTGAAGTCAGAGAGCGTGTACTTCAAGGAGAGGAACTTTCTGTTGTCTTGACCAAAATTAACATGGTTGATTTGCCATTGGGAGCTACAGAAGATAGAGTGTGTGGAACGATTGACATTGAGAAAGCCCTGACTGAGGGTGTCAAGGCATTTGAGCCTGGACTACTGGCTAAAGCTAATAGGGGAATCTTATATGTTGATGAAGTTAATCTTTTGGATGATCACTTGGTGGATGTGTTGTTGGATTCTGCTGCATCAGGATGGAACACAGTAGAGAGAGAGGGAATTTCTATCTCGCATCCTGCACGGTTTATCCTAATTGGCTCAGGCAACCCCGAAGAAGGGGAGCTCCGGCCGCAGCTGCTAGATAGGTTTGGAATGCATGCTCAAGTTGGAACTGTTAGGGATGCTGAGCTCAGAGTGAAGATTGTGGAGGAGAGAGGTCGATTTGACAAAAATCCAAAGGAATTTCGAGATTCTTACAAAGCTGAGCAAGAGAAGCTCCAACAACAAATTACCTCAGCAAGGAGTGTTCTTTCTTCTGTTCAAATTGATCAAGATCTCAAGGTGAAAATCTCCAAGGTGTGTGCTGAGTTGAATGTGGATGGATTAAGAGGAGACATAGTAACAAATAGAGCTGCAAAAGCTCTGGCTGCTCTGAAGGGAAGAGACAACGTAAGTGCCGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACACCGTCTTAGAAAGGATCCCTTGGAGTCTATAGACTCAGGTTTACTTGTCACTGAGAAATTTTATGAGGTATTCAGCTGA
Protein Sequence
  • >Glyma.13G232500.1
    MASALGTSSIAVLPSRYFSSSSSKPSIHTLSLTSGQNYGRKFYGGIGIHGIKGRAQLSVTNVATEVNSVEQAQSIASKESQRPVYPFSAIVGQDEMKLCLLLNVIDPKIGGVMIMGDRGTGKSTTVRSLVDLLPEIKVVAGDPYNSDPQDPEFMGVEVRERVLQGEELSVVLTKINMVDLPLGATEDRVCGTIDIEKALTEGVKAFEPGLLAKANRGILYVDEVNLLDDHLVDVLLDSAASGWNTVEREGISISHPARFILIGSGNPEEGELRPQLLDRFGMHAQVGTVRDAELRVKIVEERGRFDKNPKEFRDSYKAEQEKLQQQITSARSVLSSVQIDQDLKVKISKVCAELNVDGLRGDIVTNRAAKALAALKGRDNVSAEDIATVIPNCLRHRLRKDPLESIDSGLLVTEKFYEVFS*