Gene Details:
- Gene ID: Glyma.12G075700
- Gene Symbol: GmDrb2a
- Gene Name: Double-stranded RNA-binding2
- Genome: Wm82.a4.v1
- Species: Glycine max
Functional Descriptions:
- An efficient CRISPR/Cas9 reagent was used to create a bi-allelic double mutant for the two soya bean paralogous Double-stranded RNA-binding2 (GmDrb2a and GmDrb2b) genes.
- The mutated alleles in these two plants were identical (this was true for both gene targets, GmDrb2a and GmDrb2b), and at least one CRISPR/Cas9 transgene insertion was transmitted to all T1 progeny.
- The generation of the GmDrb2ab double mutant provides an excellent opportunity to characterize miRNA-directed responses to stress in soya bean and for the subsequent comparison of GmDRB2 function to the well-documented functionality of the AtDRB2 protein.
Function-related keywords:
Literature:
- CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. DOI: 10.1111/pbi.12857 ; PMID: 29087011
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Glyma.12G075700.1
GCGCGTTTCATTAATTGTCTCTCGCCCTCAAACCATGCATTATTACATCTCAAAACCCAAGAAATGAAATAAAAATATAAAAGTTAATCATACTTTTAAACTCAAAATAATTAGCAAGTTGCAGTTAGGAGGACAAACCATAATTAATTAAGATGATAAAAAAGCCAGATTAGCATCCCTGAACACATCCCATGCAACCCAAGCGTTTTTTAGTTGACTCCGACCACCATAGCAATAAATAACCCTTCTCAATTTTCAAAAATATTATTACTATTATTATTACTAATTACTAATATAATATTCACAAAATAATAAATGTACTTGTTGTTGTAAGTCCTATCTTTCTTTTCTTTTTCTCTCTTCTACCTTCTTCTTCTTCACTTAACTTTCTCTCTCTTCTCTGTTCTCCGTGCCGGTGCTGTAACTGCACGCGTGTTTTTCTGTCTTTTTGTTTTTTTTCCTCCGCCTCTCATTTGCGAAGGTGAGAACCCTAAATTTTAGAGAGAGAGGTTCGATTTGGCCGGAGGTGATTCTCCGGCGAGATTTTGCGGTGGATCAGGGTGGGGCCGGACACTTTCTTGAAGAGAGAGAGAGAGAGAGAAAGTGCGGTTTTGAGAGTTATAGAGAGAGAGAGGGGAAAATGTATAAGAACCAGCTGCAGGAGCTGGCACAGCGGAGCTGCTTCAACCTGCCTTCGTACACGTGCATTCGGGAAGGTCCTGATCACGCGCCCCGCTTCAAGGCCACCGTTAACTTTAACGGCGAGATCTTCGAGAGCCCTCATTACTGTTCAACGCTCCGTCAGGCGGAGCACTCCGCCGCCGAGGTGGCGCTCAATTCCCTCTCTAACCGTGCTCCCTCTCACTCCCTCGCCGCCAGGATCCTCGATGAAACGGGGGTGTACAAGAACCTCTTGCAGGAAATTGCGCAGAGGGTAGGGGCGCCGTTGCCGCAGTATTTTACATTCAGGTCAGGGTTAGGACATTTACCTGTTTTTACGGGGACAGTGGAGTTAGCTGGAATCATGTTTACTGGTGAACCTGCCAAGAATAAGAAACAAGCGGAGAAAAATGCAGCTATGGCCGCATGGTCTTCTCTAAAGCAATTGGCAAAAGAAACTGCAAGGTCTTCAACTGAACCCGAGAACAATGACGAACTAGAACAGATCACTATAGCACGGGCCTTACTGAACTACAGACTGAAGGAAAAGATTTCAATGTCAAATTCAAATGCTCCAGTTCCATTTCCAAAGAAGTTTCAGATTCAAAATCCCAGGCCAACCAGTCCTCAGCCCCCACCTGCTGCTACATCAAAGATTCTTCCCTTAATTTGCCAAAAGGCAGCTCCTCGAAGCAGACATCTAGTAGGAGCATCTCCAGCACGAGCTTCCTGCGACAATTCTGCGATGCCACAGCTTTCTGCTACACCAGAAAGCCGAGGAATCCGTCGCCCTAAGTTCCCTGCAGCCGGAGCAGCACCTTATGTTCCCATTAGACAAATGAGGCCTTGTCAGGGGATGGCACCTCCGGTGACAATAAGGACTGCAATACCTGTATTCTCCCCACCGCCAGCAGCAGCTGCAGTGTCCCATCAAGTACTACGAGCTCCTCATGTACGAGTTGCACCTCCGGTCACTATTCGGCAAGCTGTTCCAGTATTTGCTACTCCACCTCCTCCGATTCGAAAAGATGAACCTGTTCCCATCCCAAAAGACGAACCACCAACCATTAGTGCCCCTAGCCCGGAAGACAAACTGCCAGCTAAAACGCCAGAGGCAGAGACCAAGACTGAGAACATTCCACCTAAACCTGACATGGTAAAGAGCTTGGAGCAGCTGAAAATTTGATGTTTCATTGGAGTAGAGGTCTGAATCCATGAGAATGTGATCATGGTTTTATGATGATGTGGAGTTTGCTTCTCATGTTATAATGAGTGATGTAATGTCATCCCTTCCTTTTGCCCTGTTAAAATGCGACTGTTGGTGCTGGTTAGCATCTGCGGTCCAAATATCTGTGAAATTCAAGAGCGGTTT
CDS Sequence
- >Glyma.12G075700.1
ATGTATAAGAACCAGCTGCAGGAGCTGGCACAGCGGAGCTGCTTCAACCTGCCTTCGTACACGTGCATTCGGGAAGGTCCTGATCACGCGCCCCGCTTCAAGGCCACCGTTAACTTTAACGGCGAGATCTTCGAGAGCCCTCATTACTGTTCAACGCTCCGTCAGGCGGAGCACTCCGCCGCCGAGGTGGCGCTCAATTCCCTCTCTAACCGTGCTCCCTCTCACTCCCTCGCCGCCAGGATCCTCGATGAAACGGGGGTGTACAAGAACCTCTTGCAGGAAATTGCGCAGAGGGTAGGGGCGCCGTTGCCGCAGTATTTTACATTCAGGTCAGGGTTAGGACATTTACCTGTTTTTACGGGGACAGTGGAGTTAGCTGGAATCATGTTTACTGGTGAACCTGCCAAGAATAAGAAACAAGCGGAGAAAAATGCAGCTATGGCCGCATGGTCTTCTCTAAAGCAATTGGCAAAAGAAACTGCAAGGTCTTCAACTGAACCCGAGAACAATGACGAACTAGAACAGATCACTATAGCACGGGCCTTACTGAACTACAGACTGAAGGAAAAGATTTCAATGTCAAATTCAAATGCTCCAGTTCCATTTCCAAAGAAGTTTCAGATTCAAAATCCCAGGCCAACCAGTCCTCAGCCCCCACCTGCTGCTACATCAAAGATTCTTCCCTTAATTTGCCAAAAGGCAGCTCCTCGAAGCAGACATCTAGTAGGAGCATCTCCAGCACGAGCTTCCTGCGACAATTCTGCGATGCCACAGCTTTCTGCTACACCAGAAAGCCGAGGAATCCGTCGCCCTAAGTTCCCTGCAGCCGGAGCAGCACCTTATGTTCCCATTAGACAAATGAGGCCTTGTCAGGGGATGGCACCTCCGGTGACAATAAGGACTGCAATACCTGTATTCTCCCCACCGCCAGCAGCAGCTGCAGTGTCCCATCAAGTACTACGAGCTCCTCATGTACGAGTTGCACCTCCGGTCACTATTCGGCAAGCTGTTCCAGTATTTGCTACTCCACCTCCTCCGATTCGAAAAGATGAACCTGTTCCCATCCCAAAAGACGAACCACCAACCATTAGTGCCCCTAGCCCGGAAGACAAACTGCCAGCTAAAACGCCAGAGGCAGAGACCAAGACTGAGAACATTCCACCTAAACCTGACATGGTAAAGAGCTTGGAGCAGCTGAAAATTTGA
Protein Sequence
- >Glyma.12G075700.1
MYKNQLQELAQRSCFNLPSYTCIREGPDHAPRFKATVNFNGEIFESPHYCSTLRQAEHSAAEVALNSLSNRAPSHSLAARILDETGVYKNLLQEIAQRVGAPLPQYFTFRSGLGHLPVFTGTVELAGIMFTGEPAKNKKQAEKNAAMAAWSSLKQLAKETARSSTEPENNDELEQITIARALLNYRLKEKISMSNSNAPVPFPKKFQIQNPRPTSPQPPPAATSKILPLICQKAAPRSRHLVGASPARASCDNSAMPQLSATPESRGIRRPKFPAAGAAPYVPIRQMRPCQGMAPPVTIRTAIPVFSPPPAAAAVSHQVLRAPHVRVAPPVTIRQAVPVFATPPPPIRKDEPVPIPKDEPPTISAPSPEDKLPAKTPEAETKTENIPPKPDMVKSLEQLKI*