Information report for GmDrb2a
Gene Details
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Functional Descriptions
- An efficient CRISPR/Cas9 reagent was used to create a bi-allelic double mutant for the two soya bean paralogous Double-stranded RNA-binding2 (GmDrb2a and GmDrb2b) genes.
- The mutated alleles in these two plants were identical (this was true for both gene targets, GmDrb2a and GmDrb2b), and at least one CRISPR/Cas9 transgene insertion was transmitted to all T1 progeny.
- The generation of the GmDrb2ab double mutant provides an excellent opportunity to characterize miRNA-directed responses to stress in soya bean and for the subsequent comparison of GmDRB2 function to the well-documented functionality of the AtDRB2 protein.
Literature and News
Gene Resources
- NCBI ID: 100812728
- UniProt accessions: C6TJ54
- EMBL: BT097697 , CM000845
- EnsemblPlants: KRH25014
- Gramene: KRH25014
- KEGG: gmx:100812728
- GO: GO:0003725
- OMA: KKFFMQN
- InterPro: IPR014720 , IPR044450 , IPR044451
- PANTHER: PTHR46031 , PTHR46031:SF26
- SUPFAM: SSF54768
- PROSITE: PS50137
- Gene3D: 3.30.160.20
- OrthoDB: C6TJ54
- SMR: C6TJ54
- CDD: cd19907 , cd19908
Sequences
cDNA Sequence
- >Glyma.12G075700.1
GCGCGTTTCATTAATTGTCTCTCGCCCTCAAACCATGCATTATTACATCTCAAAACCCAAGAAATGAAATAAAAATATAAAAGTTAATCATACTTTTAAACTCAAAATAATTAGCAAGTTGCAGTTAGGAGGACAAACCATAATTAATTAAGATGATAAAAAAGCCAGATTAGCATCCCTGAACACATCCCATGCAACCCAAGCGTTTTTTAGTTGACTCCGACCACCATAGCAATAAATAACCCTTCTCAATTTTCAAAAATATTATTACTATTATTATTACTAATTACTAATATAATATTCACAAAATAATAAATGTACTTGTTGTTGTAAGTCCTATCTTTCTTTTCTTTTTCTCTCTTCTACCTTCTTCTTCTTCACTTAACTTTCTCTCTCTTCTCTGTTCTCCGTGCCGGTGCTGTAACTGCACGCGTGTTTTTCTGTCTTTTTGTTTTTTTTCCTCCGCCTCTCATTTGCGAAGGTGAGAACCCTAAATTTTAGAGAGAGAGGTTCGATTTGGCCGGAGGTGATTCTCCGGCGAGATTTTGCGGTGGATCAGGGTGGGGCCGGACACTTTCTTGAAGAGAGAGAGAGAGAGAGAAAGTGCGGTTTTGAGAGTTATAGAGAGAGAGAGGGGAAAATGTATAAGAACCAGCTGCAGGAGCTGGCACAGCGGAGCTGCTTCAACCTGCCTTCGTACACGTGCATTCGGGAAGGTCCTGATCACGCGCCCCGCTTCAAGGCCACCGTTAACTTTAACGGCGAGATCTTCGAGAGCCCTCATTACTGTTCAACGCTCCGTCAGGCGGAGCACTCCGCCGCCGAGGTGGCGCTCAATTCCCTCTCTAACCGTGCTCCCTCTCACTCCCTCGCCGCCAGGATCCTCGATGAAACGGGGGTGTACAAGAACCTCTTGCAGGAAATTGCGCAGAGGGTAGGGGCGCCGTTGCCGCAGTATTTTACATTCAGGTCAGGGTTAGGACATTTACCTGTTTTTACGGGGACAGTGGAGTTAGCTGGAATCATGTTTACTGGTGAACCTGCCAAGAATAAGAAACAAGCGGAGAAAAATGCAGCTATGGCCGCATGGTCTTCTCTAAAGCAATTGGCAAAAGAAACTGCAAGGTCTTCAACTGAACCCGAGAACAATGACGAACTAGAACAGATCACTATAGCACGGGCCTTACTGAACTACAGACTGAAGGAAAAGATTTCAATGTCAAATTCAAATGCTCCAGTTCCATTTCCAAAGAAGTTTCAGATTCAAAATCCCAGGCCAACCAGTCCTCAGCCCCCACCTGCTGCTACATCAAAGATTCTTCCCTTAATTTGCCAAAAGGCAGCTCCTCGAAGCAGACATCTAGTAGGAGCATCTCCAGCACGAGCTTCCTGCGACAATTCTGCGATGCCACAGCTTTCTGCTACACCAGAAAGCCGAGGAATCCGTCGCCCTAAGTTCCCTGCAGCCGGAGCAGCACCTTATGTTCCCATTAGACAAATGAGGCCTTGTCAGGGGATGGCACCTCCGGTGACAATAAGGACTGCAATACCTGTATTCTCCCCACCGCCAGCAGCAGCTGCAGTGTCCCATCAAGTACTACGAGCTCCTCATGTACGAGTTGCACCTCCGGTCACTATTCGGCAAGCTGTTCCAGTATTTGCTACTCCACCTCCTCCGATTCGAAAAGATGAACCTGTTCCCATCCCAAAAGACGAACCACCAACCATTAGTGCCCCTAGCCCGGAAGACAAACTGCCAGCTAAAACGCCAGAGGCAGAGACCAAGACTGAGAACATTCCACCTAAACCTGACATGGTAAAGAGCTTGGAGCAGCTGAAAATTTGATGTTTCATTGGAGTAGAGGTCTGAATCCATGAGAATGTGATCATGGTTTTATGATGATGTGGAGTTTGCTTCTCATGTTATAATGAGTGATGTAATGTCATCCCTTCCTTTTGCCCTGTTAAAATGCGACTGTTGGTGCTGGTTAGCATCTGCGGTCCAAATATCTGTGAAATTCAAGAGCGGTTT
CDS Sequence
- >Glyma.12G075700.1
ATGTATAAGAACCAGCTGCAGGAGCTGGCACAGCGGAGCTGCTTCAACCTGCCTTCGTACACGTGCATTCGGGAAGGTCCTGATCACGCGCCCCGCTTCAAGGCCACCGTTAACTTTAACGGCGAGATCTTCGAGAGCCCTCATTACTGTTCAACGCTCCGTCAGGCGGAGCACTCCGCCGCCGAGGTGGCGCTCAATTCCCTCTCTAACCGTGCTCCCTCTCACTCCCTCGCCGCCAGGATCCTCGATGAAACGGGGGTGTACAAGAACCTCTTGCAGGAAATTGCGCAGAGGGTAGGGGCGCCGTTGCCGCAGTATTTTACATTCAGGTCAGGGTTAGGACATTTACCTGTTTTTACGGGGACAGTGGAGTTAGCTGGAATCATGTTTACTGGTGAACCTGCCAAGAATAAGAAACAAGCGGAGAAAAATGCAGCTATGGCCGCATGGTCTTCTCTAAAGCAATTGGCAAAAGAAACTGCAAGGTCTTCAACTGAACCCGAGAACAATGACGAACTAGAACAGATCACTATAGCACGGGCCTTACTGAACTACAGACTGAAGGAAAAGATTTCAATGTCAAATTCAAATGCTCCAGTTCCATTTCCAAAGAAGTTTCAGATTCAAAATCCCAGGCCAACCAGTCCTCAGCCCCCACCTGCTGCTACATCAAAGATTCTTCCCTTAATTTGCCAAAAGGCAGCTCCTCGAAGCAGACATCTAGTAGGAGCATCTCCAGCACGAGCTTCCTGCGACAATTCTGCGATGCCACAGCTTTCTGCTACACCAGAAAGCCGAGGAATCCGTCGCCCTAAGTTCCCTGCAGCCGGAGCAGCACCTTATGTTCCCATTAGACAAATGAGGCCTTGTCAGGGGATGGCACCTCCGGTGACAATAAGGACTGCAATACCTGTATTCTCCCCACCGCCAGCAGCAGCTGCAGTGTCCCATCAAGTACTACGAGCTCCTCATGTACGAGTTGCACCTCCGGTCACTATTCGGCAAGCTGTTCCAGTATTTGCTACTCCACCTCCTCCGATTCGAAAAGATGAACCTGTTCCCATCCCAAAAGACGAACCACCAACCATTAGTGCCCCTAGCCCGGAAGACAAACTGCCAGCTAAAACGCCAGAGGCAGAGACCAAGACTGAGAACATTCCACCTAAACCTGACATGGTAAAGAGCTTGGAGCAGCTGAAAATTTGA
Protein Sequence
- >Glyma.12G075700.1
MYKNQLQELAQRSCFNLPSYTCIREGPDHAPRFKATVNFNGEIFESPHYCSTLRQAEHSAAEVALNSLSNRAPSHSLAARILDETGVYKNLLQEIAQRVGAPLPQYFTFRSGLGHLPVFTGTVELAGIMFTGEPAKNKKQAEKNAAMAAWSSLKQLAKETARSSTEPENNDELEQITIARALLNYRLKEKISMSNSNAPVPFPKKFQIQNPRPTSPQPPPAATSKILPLICQKAAPRSRHLVGASPARASCDNSAMPQLSATPESRGIRRPKFPAAGAAPYVPIRQMRPCQGMAPPVTIRTAIPVFSPPPAAAAVSHQVLRAPHVRVAPPVTIRQAVPVFATPPPPIRKDEPVPIPKDEPPTISAPSPEDKLPAKTPEAETKTENIPPKPDMVKSLEQLKI*