Information report for GmFAD2–1A
Gene Details
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Functional Descriptions
- Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean.
- The fatty acid profile analysis of T1 seeds derived from CRISPR-edited plants homozygous for both GmFAD2 genes showed dramatic increases in oleic acid content to over 80%, whereas linoleic acid decreased to 1.3–1.7%.
- Palmitic and linolenic acid levels were also significantly reduced in the double GmFAD2 mutants, whereas stearic acid levels were comparable to wildtype.
- Using the GmFAD2- 1A and GmFAD-1B genes are targets, we demonstrated that dual gRNA CRISPR/Cas9 system induced high frequency of heritable mutations in homeologous soybean genes.
Functional Keywords
Literature and News
Gene Resources
- NCBI ID: 547814
- UniProt accessions: Q5FBA1
- EMBL: AB188250 , CM000843 , KX666091
- AlphaFoldDB: Q5FBA1
- KEGG: gmx:547814
- GO: GO:0006629 , GO:0016020 , GO:0016491
- OMA: MEATNAM
- InterPro: IPR005804 , IPR012171
- PANTHER: PTHR32100
- OrthoDB: Q5FBA1
- SMR: Q5FBA1
- CDD: cd03507
Sequences
cDNA Sequence
- >Glyma.10G278000.1
CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC - >Glyma.10G278000.2
CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC - >Glyma.10G278000.3
CTCCTAGTTTCCTAACCATATACTAATATTTGCTTGTATTGATAGCCCCTCCGTTCCCAAGAGTATAAAACTGCATCGAATAATACAAGCCACTAGGCATGGTAAATTAAATTGTGCCTGCACCTCGGGATATTTCATGTGGGGTTCATCATATTTGTTGAGGAAAAGAAACTCCCGAAATTGAATTATGCATTTATATATCCTTTTTCATTTCTAGATTTCCTGAAGGCTTAGGTGTAGGCACCTAGCTAGTAGCTACAATATCAGCACTTCTCTCTATTGATAAACAATTGGCTGTAATGCCGCAGTAGAGGACGATCACAACATTTCGTGCTGGTTACTTTTTGTTTTATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGATGGAGCAACCAATGGGCCATAGTGGGAGTTATGGAAGTTTTGTCATGTATTAGTACATAATTAGTAGAATGTTATAAATAAGTGGATTTGCCGCGTAATGACTTTGTGTGTATTGTGAAACAGCTTGTTGCGATCATGGTTATAATGTAAAAATAATTCTGGTATTAATTACATGTGGAAAGTGTTCTGC
CDS Sequence
- >Glyma.10G278000.1
ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA - >Glyma.10G278000.2
ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA - >Glyma.10G278000.3
ATGGGTCTAGCAAAGGAAACAACAATGGGAGGTAGAGGTCGTGTGGCCAAAGTGGAAGTTCAAGGGAAGAAGCCTCTCTCAAGGGTTCCAAACACAAAGCCACCATTCACTGTTGGCCAACTCAAGAAAGCAATTCCACCACACTGCTTTCAGCGCTCCCTCCTCACTTCATTCTCCTATGTTGTTTATGACCTTTCATTTGCCTTCATTTTCTACATTGCCACCACCTACTTCCACCTCCTTCCTCAACCCTTTTCCCTCATTGCATGGCCAATCTATTGGGTTCTCCAAGGTTGCCTTCTCACTGGTGTGTGGGTGATTGCTCACGAGTGTGGTCACCATGCCTTCAGCAAGTACCAATGGGTTGATGATGTTGTGGGTTTGACCCTTCACTCAACACTTTTAGTCCCTTATTTCTCATGGAAAATAAGCCATCGCCGCCATCACTCCAACACAGGTTCCCTTGACCGTGATGAAGTGTTTGTCCCAAAACCAAAATCCAAAGTTGCATGGTTTTCCAAGTACTTAAACAACCCTCTAGGAAGGGCTGTTTCTCTTCTCGTCACACTCACAATAGGGTGGCCTATGTATTTAGCCTTCAATGTCTCTGGTAGACCCTATGATAGTTTTGCAAGCCACTACCACCCTTATGCTCCCATATATTCTAACCGTGAGAGGCTTCTGATCTATGTCTCTGATGTTGCTTTGTTTTCTGTGACTTACTCTCTCTACCGTGTTGCAACCCTGAAAGGGTTGGTTTGGCTGCTATGTGTTTATGGGGTGCCTTTGCTCATTGTGAACGGTTTTCTTGTGACTATCACATATTTGCAGCACACACACTTTGCCTTGCCTCATTACGATTCATCAGAATGGGACTGGCTGAAGGGAGCTTTGGCAACTATGGACAGAGATTATGGGATTCTGAACAAGGTGTTTCATCACATAACTGATACTCATGTGGCTCACCATCTCTTCTCTACAATGCCACATTACCATGCAATGGAGGCAACCAATGCAATCAAGCCAATATTGGGTGAGTACTACCAATTTGATGACACACCATTTTACAAGGCACTGTGGAGAGAAGCGAGAGAGTGCCTCTATGTGGAGCCAGATGAAGGAACATCCGAGAAGGGCGTGTATTGGTACAGGAACAAGTATTGA
Protein Sequence
- >Glyma.10G278000.1
MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY* - >Glyma.10G278000.2
MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY* - >Glyma.10G278000.3
MGLAKETTMGGRGRVAKVEVQGKKPLSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYDLSFAFIFYIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSNTGSLDRDEVFVPKPKSKVAWFSKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFASHYHPYAPIYSNRERLLIYVSDVALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPDEGTSEKGVYWYRNKY*