Gene Details:

• Gene ID: Glyma.10G278000
• Gene Symbol: GmFAD2–1A
• Gene Name: Fatty Acid Desaturase 2
• Genome: Wm82.a4.v1
• Species: Glycine max

Functional Descriptions:

• Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean.
• The fatty acid profile analysis of T1 seeds derived from CRISPR-edited plants homozygous for both GmFAD2 genes showed dramatic increases in oleic acid content to over 80%, whereas linoleic acid decreased to 1.3–1.7%.
• Palmitic and linolenic acid levels were also significantly reduced in the double GmFAD2 mutants, whereas stearic acid levels were comparable to wildtype.
• Using the GmFAD2- 1A and GmFAD-1B genes are targets, we demonstrated that dual gRNA CRISPR/Cas9 system induced high frequency of heritable mutations in homeologous soybean genes.

Function-related keywords:

• yield ,  fatty-acid

Literature:

• Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2-1A and GmFAD2-1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean.   DOI:  10.1186/s12870-019-1906-8 ;  PMID:  31307375

Related News:

Gene Resources:

• NCBI ID: 547814
• UniProt accessions: Q5FBA1

Orthologs:

Sequences: