Gene Details:

  • Gene ID: Glyma.06g186300
  • Gene Symbol: GmAMADH2
  • Gene Name: amino aldehyde dehydrogenase2
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Deficiency in the amino aldehyde dehydrogenase encoded by GmAMADH2, the homologue of rice Os2AP, enhances 2-acetyl-1-pyrroline biosynthesis in soybeans.
  • This variation caused a frame-shift mutation and a premature stop codon. Suppression of GmAMADH2 by introduction of a GmAMADH2-RNAi construct into the calli of the two nonaromatic wild-type varieties inhibited the synthesis of AMADH and induced the biosynthesis of 2AP.
  • GmAMADH2 is the key gene responsible for 2AP biosynthesis in soybeans.
  • These results suggest that deficiency in the GmAMADH2 product, AMADH, plays a similar role in soybean as in rice, which is to promote 2AP biosynthesis. This phenomenon might be a conserved mechanism among plant species.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.06G186300.1
    ATCCAATCAAGAATACAATAACATAGCATAGAGTGAGAAGTGAGAAGAGAAAAATGGCAATCTCCATACCCAGTAGACAACTTTTCATAGATGGAGAGTGGAAAGTCCCTCTTCTCAACAATCGGTTTCCGATCATCAACCCCGCTACTGAAGACATCATAGGGCATATTCCAGCCGCTACTAAGGAAGATGTTGACCTTGCGGTGGATGCTGCTAAAAGAGCCTTTTCCCATAACAAAGGCAAAGATTGGTCTTCAGCTCCTGGCTCTGTTCGTGCTCGCTATCTTCGAGCCATCGCTTCAAAGATAACAGAGAAAAAGGATGAACTAGGAAAGCTTGAAGCAATTGATTGTGGAAAACCACTAGATGAAGCACTGGCAGACCTGGATGATGTTATTGGTTGTTTTAACTACTATGCCGAGCTTGCAGAAGGATTGGACGCAAAGCAAAATGCTCCTGTATCCCTTCCTATGGAAACCTTCAAGAGTTATGTTCTCAAGGAGCCCATCGGAGTTGTTGCATTAATTACTCCATGGAACTATCCTCTATTGATGGCTACATGGAAAGTTGCTCCTGCTCTAGCTGCTGGTTGTACAGCAATATTGAAGCCATCTGAATTGGCATCTGTGACCTGTTTGGAGCTGGCTGAAATATGCAGAGAAGTTGGCCTTCCTCCAGGGGTATTAAACATTGTTACTGGATTAGGCAATGAAGCTGGTGCTCCTTTGTCATCTCATCCTGACGTAGACAAGATTTCCTTTACTGGAAGCTCTGCAACTGGAAGCAGGATTATGACAGCTGCAGCACAGCTAACGAAGCCTGTTTCTCTAGAGCTTGGTGGAAAAAGCCCAATCATTGTTTTTGAGGATGTTGACCTTGATAAGACTGCTGAATGGACCATCTTTGGCTGCTTCTTTACAAATGGTCAGATATGCAGTGCAACATCTCGCCTTATTGTGCATGAAAGTATAGCAACAGAATTTGTGAATAGACTTGTGCAATGGGCCAAAAACATCAAAATTTCAGATCCCTTTGAAGAAGGTTGTAGGCTAGGTCCTATTGTTAGTGAAGGACAGTATAAAAAAGTGTTGAATTGTATCTCAACTGCTAAGAGTGAGGGTGCAACAATTTTGATTGGAGGGTCTCGCCCAGAGCATTTGAAAAAGGGGTACTTTGTTGAACCAACCATCATAACTGATGTGACTACCTCGATGCAAATTTGGAGAGAAGAAGTTTTTGGACCTGTGCTATGTGTGAAAACATTCAGTACCGAAGAAGAAGCTATTGAACTAGCAAATGACACACACTACGGCTTAGGGTCTGCTGTAATGTCAAAAGATCTAGAAAGATGCGAGCGCATATCAAAGGCTATTCAGGCTGGAATTGTATGGATAAATTGTGCTCAACCAAGCTTCATTCAAGCTCCATGGGGAGGCGTTAAACGTAGTGGTTTTGGTCGTGAACTAGGAGAATGGGGACTCGAGAACTATTTAAGTGTGAAGCAAGTGACCAAGTACATATCTGATGAACCGTGGGGCTGGTATCAGTCGCCTTCAAAGCTGTGATATGAATTGCTGTTCTAAAATCTGGCATGAATAAGTTGTGGGGGACATTAATTTGTGATGATGATCTTAATTCGAGAAAATAATGTGCATATGTATAACACTGAAATTGTTCCATGGCCTTGTTAATGACGGGCTGAGTTGATTTTATCTTATTTGTGATGATGATCTTCACGTTAGAACTTTTTTTTTACAATGTTTACATGTGATTTCTTAAATGTTTTTGTTGTGTTTTCTAGTTAAAATTGGAGTTTCACACAAGTAACTGATACTCATCTATAATGCAATTTTTTATTGCATAAATTAGTGAAGTGAAACTCTAATTATGATTAAAAAATAATAC
  • >Glyma.06G186300.4
    ATCCAATCAAGAATACAATAACATAGCATAGAGTGAGAAGTGAGAAGAGAAAAATGGCAATCTCCATACCCAGTAGACAACTTTTCATAGATGGAGAGTGGAAAGTCCCTCTTCTCAACAATCGGTTTCCGATCATCAACCCCGCTACTGAAGACATCATAGGGCATATTCCAGCCGCTACTAAGGAAGATGTTGACCTTGCGGTGGATGCTGCTAAAAGAGCCTTTTCCCATAACAAAGGCAAAGATTGGTCTTCAGCTCCTGGCTCTGTTCGTGCTCGCTATCTTCGAGCCATCGCTTCAAAGATAACAGAGAAAAAGGATGAACTAGGAAAGCTTGAAGCAATTGATTGTGGAAAACCACTAGATGAAGCACTGGCAGACCTGGATGATGTTATTGGTTGTTTTAACTACTATGCCGAGCTTGCAGAAGGATTGGACGCAAAGCAAAATGCTCCTGTATCCCTTCCTATGGAAACCTTCAAGAGTTATGTTCTCAAGGAGCCCATCGGAGTTGTTGCATTAATTACTCCATGGAACTATCCTCTATTGATGGCTACATGGAAAGTTGCTCCTGCTCTAGCTGCTGGTTGTACAGCAATATTGAAGCCATCTGAATTGGCATCTGTGACCTGTTTGGAGCTGGCTGAAATATGCAGAGAAGTTGGCCTTCCTCCAGGGGTATTAAACATTGTTACTGGATTAGGCAATGAAGCTGGTGCTCCTTTGTCATCTCATCCTGACGTAGACAAGATTTCCTTTACTGGAAGCTCTGCAACTGGAAGCAGGATTATGACAGCTGCAGCACAGCTAACGAAGCCTGTTTCTCTAGAGCTTGGTGGAAAAAGCCCAATCATTGTTTTTGAGGATGTTGACCTTGATAAGACTGCTGAATGGACCATCTTTGGCTGCTTCTTTACAAATGGTCAGATATGCAGTGCAACATCTCGCCTTATTGTGCATGAAAGTATAGCAACAGAATTTGTGAATAGACTTGTGCAATGGGCCAAAAACATCAAAATTTCAGATCCCTTTGAAGAAGGTTGTAGGCTAGGTCCTATTGTTAGTGAAGGACAGTATAAAAAAGTGTTGAATTGTATCTCAACTGCTAAGAGTGAGGGTGCAACAATTTTGATTGGAGGGTCTCGCCCAGAGCATTTGAAAAAGGGGTACTTTGTTGAACCAACCATCATAACTGATGTGACTACCTCGATGCAAATTTGGAGAGAAGAAGTTTTTGGACCTGTGCTATGTGTGAAAACATTCAGTACCGAAGAAGAAGCTATTGAACTAGCAAATGACACACACTACGGCTTAGGGTCTGCTGTAATGTCAAAAGATCTAGAAAGATGCGAGCGCATATCAAAGGCTATTCAGGCTGGAATTGTATGGATAAATTGTGCTCAACCAAGCTTCATTCAAGCTCCATGGGGAGGCGTTAAACGTAGTGGTTTTGGTCGTGAACTAGGAGAATGGGGACTCGAGAACTATTTAAGTGTGAAGCAAGTGACCAAGTACATATCTGATGAACCGTGGGGCTGGTATCAGTCGCCTTCAAAGCTGTGATATGAATTGCTGTTCTAAAATCTGGCATGAATAAGTTGTGGGGGACATTAATTTGTGATGATGATCTTAATTCGAGAAAATAATGTGCATATGTATAACACTGAAATTGTTCCATGGCCTTGTTAATGACGGGCTGAGTTGATTTTATCTTATTTGTGATGATGATCTTCACGTTAGAACTTTTTTTTTACAATGTTTACATGTGATTTCTTAAATGTTTTTGTTGTGTTTTCTAGTTAAAATTGGAGTTTCACACAAGTAACTGATACTCATCTATAATGCAATTTTTTATTGCATAAATTAGTGAAGTGAAACTCTAATTATGATTAAAAAATAATAC
CDS Sequence
  • >Glyma.06G186300.1
    ATGGCAATCTCCATACCCAGTAGACAACTTTTCATAGATGGAGAGTGGAAAGTCCCTCTTCTCAACAATCGGTTTCCGATCATCAACCCCGCTACTGAAGACATCATAGGGCATATTCCAGCCGCTACTAAGGAAGATGTTGACCTTGCGGTGGATGCTGCTAAAAGAGCCTTTTCCCATAACAAAGGCAAAGATTGGTCTTCAGCTCCTGGCTCTGTTCGTGCTCGCTATCTTCGAGCCATCGCTTCAAAGATAACAGAGAAAAAGGATGAACTAGGAAAGCTTGAAGCAATTGATTGTGGAAAACCACTAGATGAAGCACTGGCAGACCTGGATGATGTTATTGGTTGTTTTAACTACTATGCCGAGCTTGCAGAAGGATTGGACGCAAAGCAAAATGCTCCTGTATCCCTTCCTATGGAAACCTTCAAGAGTTATGTTCTCAAGGAGCCCATCGGAGTTGTTGCATTAATTACTCCATGGAACTATCCTCTATTGATGGCTACATGGAAAGTTGCTCCTGCTCTAGCTGCTGGTTGTACAGCAATATTGAAGCCATCTGAATTGGCATCTGTGACCTGTTTGGAGCTGGCTGAAATATGCAGAGAAGTTGGCCTTCCTCCAGGGGTATTAAACATTGTTACTGGATTAGGCAATGAAGCTGGTGCTCCTTTGTCATCTCATCCTGACGTAGACAAGATTTCCTTTACTGGAAGCTCTGCAACTGGAAGCAGGATTATGACAGCTGCAGCACAGCTAACGAAGCCTGTTTCTCTAGAGCTTGGTGGAAAAAGCCCAATCATTGTTTTTGAGGATGTTGACCTTGATAAGACTGCTGAATGGACCATCTTTGGCTGCTTCTTTACAAATGGTCAGATATGCAGTGCAACATCTCGCCTTATTGTGCATGAAAGTATAGCAACAGAATTTGTGAATAGACTTGTGCAATGGGCCAAAAACATCAAAATTTCAGATCCCTTTGAAGAAGGTTGTAGGCTAGGTCCTATTGTTAGTGAAGGACAGTATAAAAAAGTGTTGAATTGTATCTCAACTGCTAAGAGTGAGGGTGCAACAATTTTGATTGGAGGGTCTCGCCCAGAGCATTTGAAAAAGGGGTACTTTGTTGAACCAACCATCATAACTGATGTGACTACCTCGATGCAAATTTGGAGAGAAGAAGTTTTTGGACCTGTGCTATGTGTGAAAACATTCAGTACCGAAGAAGAAGCTATTGAACTAGCAAATGACACACACTACGGCTTAGGGTCTGCTGTAATGTCAAAAGATCTAGAAAGATGCGAGCGCATATCAAAGGCTATTCAGGCTGGAATTGTATGGATAAATTGTGCTCAACCAAGCTTCATTCAAGCTCCATGGGGAGGCGTTAAACGTAGTGGTTTTGGTCGTGAACTAGGAGAATGGGGACTCGAGAACTATTTAAGTGTGAAGCAAGTGACCAAGTACATATCTGATGAACCGTGGGGCTGGTATCAGTCGCCTTCAAAGCTGTGA
  • >Glyma.06G186300.4
    ATGGCAATCTCCATACCCAGTAGACAACTTTTCATAGATGGAGAGTGGAAAGTCCCTCTTCTCAACAATCGGTTTCCGATCATCAACCCCGCTACTGAAGACATCATAGGGCATATTCCAGCCGCTACTAAGGAAGATGTTGACCTTGCGGTGGATGCTGCTAAAAGAGCCTTTTCCCATAACAAAGGCAAAGATTGGTCTTCAGCTCCTGGCTCTGTTCGTGCTCGCTATCTTCGAGCCATCGCTTCAAAGATAACAGAGAAAAAGGATGAACTAGGAAAGCTTGAAGCAATTGATTGTGGAAAACCACTAGATGAAGCACTGGCAGACCTGGATGATGTTATTGGTTGTTTTAACTACTATGCCGAGCTTGCAGAAGGATTGGACGCAAAGCAAAATGCTCCTGTATCCCTTCCTATGGAAACCTTCAAGAGTTATGTTCTCAAGGAGCCCATCGGAGTTGTTGCATTAATTACTCCATGGAACTATCCTCTATTGATGGCTACATGGAAAGTTGCTCCTGCTCTAGCTGCTGGTTGTACAGCAATATTGAAGCCATCTGAATTGGCATCTGTGACCTGTTTGGAGCTGGCTGAAATATGCAGAGAAGTTGGCCTTCCTCCAGGGGTATTAAACATTGTTACTGGATTAGGCAATGAAGCTGGTGCTCCTTTGTCATCTCATCCTGACGTAGACAAGATTTCCTTTACTGGAAGCTCTGCAACTGGAAGCAGGATTATGACAGCTGCAGCACAGCTAACGAAGCCTGTTTCTCTAGAGCTTGGTGGAAAAAGCCCAATCATTGTTTTTGAGGATGTTGACCTTGATAAGACTGCTGAATGGACCATCTTTGGCTGCTTCTTTACAAATGGTCAGATATGCAGTGCAACATCTCGCCTTATTGTGCATGAAAGTATAGCAACAGAATTTGTGAATAGACTTGTGCAATGGGCCAAAAACATCAAAATTTCAGATCCCTTTGAAGAAGGTTGTAGGCTAGGTCCTATTGTTAGTGAAGGACAGTATAAAAAAGTGTTGAATTGTATCTCAACTGCTAAGAGTGAGGGTGCAACAATTTTGATTGGAGGGTCTCGCCCAGAGCATTTGAAAAAGGGGTACTTTGTTGAACCAACCATCATAACTGATGTGACTACCTCGATGCAAATTTGGAGAGAAGAAGTTTTTGGACCTGTGCTATGTGTGAAAACATTCAGTACCGAAGAAGAAGCTATTGAACTAGCAAATGACACACACTACGGCTTAGGGTCTGCTGTAATGTCAAAAGATCTAGAAAGATGCGAGCGCATATCAAAGGCTATTCAGGCTGGAATTGTATGGATAAATTGTGCTCAACCAAGCTTCATTCAAGCTCCATGGGGAGGCGTTAAACGTAGTGGTTTTGGTCGTGAACTAGGAGAATGGGGACTCGAGAACTATTTAAGTGTGAAGCAAGTGACCAAGTACATATCTGATGAACCGTGGGGCTGGTATCAGTCGCCTTCAAAGCTGTGA
Protein Sequence
  • >Glyma.06G186300.1
    MAISIPSRQLFIDGEWKVPLLNNRFPIINPATEDIIGHIPAATKEDVDLAVDAAKRAFSHNKGKDWSSAPGSVRARYLRAIASKITEKKDELGKLEAIDCGKPLDEALADLDDVIGCFNYYAELAEGLDAKQNAPVSLPMETFKSYVLKEPIGVVALITPWNYPLLMATWKVAPALAAGCTAILKPSELASVTCLELAEICREVGLPPGVLNIVTGLGNEAGAPLSSHPDVDKISFTGSSATGSRIMTAAAQLTKPVSLELGGKSPIIVFEDVDLDKTAEWTIFGCFFTNGQICSATSRLIVHESIATEFVNRLVQWAKNIKISDPFEEGCRLGPIVSEGQYKKVLNCISTAKSEGATILIGGSRPEHLKKGYFVEPTIITDVTTSMQIWREEVFGPVLCVKTFSTEEEAIELANDTHYGLGSAVMSKDLERCERISKAIQAGIVWINCAQPSFIQAPWGGVKRSGFGRELGEWGLENYLSVKQVTKYISDEPWGWYQSPSKL*
  • >Glyma.06G186300.4
    MAISIPSRQLFIDGEWKVPLLNNRFPIINPATEDIIGHIPAATKEDVDLAVDAAKRAFSHNKGKDWSSAPGSVRARYLRAIASKITEKKDELGKLEAIDCGKPLDEALADLDDVIGCFNYYAELAEGLDAKQNAPVSLPMETFKSYVLKEPIGVVALITPWNYPLLMATWKVAPALAAGCTAILKPSELASVTCLELAEICREVGLPPGVLNIVTGLGNEAGAPLSSHPDVDKISFTGSSATGSRIMTAAAQLTKPVSLELGGKSPIIVFEDVDLDKTAEWTIFGCFFTNGQICSATSRLIVHESIATEFVNRLVQWAKNIKISDPFEEGCRLGPIVSEGQYKKVLNCISTAKSEGATILIGGSRPEHLKKGYFVEPTIITDVTTSMQIWREEVFGPVLCVKTFSTEEEAIELANDTHYGLGSAVMSKDLERCERISKAIQAGIVWINCAQPSFIQAPWGGVKRSGFGRELGEWGLENYLSVKQVTKYISDEPWGWYQSPSKL*