Gene Details:

  • Gene ID: Glyma.03g196800
  • Gene Symbol: GmRPA3b
  • Gene Name: Replication Protein A subunit 3
  • Genome: Wm82.a4.v1
  • Species: Glycine max

Functional Descriptions:

  • Replication protein A subunit 3 and the iron efficiency response in soybean.
  • We used virus induced gene silencing (VIGS) to repress GmRPA3 expression in the iron inefficient line Isoclark and mirror expression in Clark.
  • GmRPA3-silenced plants had improved IDC symptoms and chlorophyll content under iron deficient conditions and also displayed stunted growth regardless of iron availability.
  • RNA-Seq comparing gene expression between GmRPA3-silenced and empty vector plants revealed massive transcriptional reprogramming with differential expression of genes associated with defense, immunity, aging, death, protein modification, protein synthesis, photosynthesis and iron uptake and transport genes.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Glyma.03G196800.2
    TTGAGCCGCATATAAGATATTTTCTCGCAACTTGTGTCAACTGTTGGTATATATGAGCTAATTTTAACTTATAAAAAGTTTTTTTTTCTATTATCTTCTCCTAAAATTGCTTTTAAATTTCTTAATAATAATAAAAAGTTTATACAAATATGCTTGTGTAAGAAGGCCCATGCCAAAAGCCTCCTGACACTGCCGAGCAACAGTGAATTTGGCCACCATACCATACCATGAGCATAAGGGCGCGCGCGCGCAAAGGATTAGGGTTTCGAACGTAGCGCTTTTGGAGCTTCCATTTCGATTTATACTTGCGCACGATCAGTTAAGGAGGGAGGGCTTTGGAGTTGAATGCATTGCTCGTTTTTACTGAAAGGAGAACCCGCGCTCTCTTTGGTTGCCGCTTTTTTTCTGTGTTCTGAATTCAATCAGCACTGAGCATTGATCATTGAGCATTCAGCATTGAGCAGATGGATATATCAAATCCTGCAGCCTTAGTCAATGCGGAACTGTTGCACTTCTATGTTGGAAGAAGGGTAAGGATCGTGGTGCAGGTTGTGCGATCTGATGGTGGAGTTGTCATTGGAAAATCAACAGATGAGAAACAACTAGTTGTAAAAGGATCACCCCCACCTGCTGCTCCTCTTACAACTTTTGTTGAAGTTTATGGTCTTGTTAACAGTGATAAGTCAATTGGTGCTGAGATATGGACCAATTTTGGTGATGCCATCGATATGGATAGCTACAATAAGCTTTGTCAGCTCGCAAATGGAGAATTTAAACACTTGTTCCTGTGAGTTCTAACAAGCTTGTCTGATGATACAGAGGCCTTTCTATCATTTGGAATACTAGAGGAGACATAATCAATGTGTTGGCAACAATGTTTTTCATTTTTTTTTTCCTGATATAATGGTGACAATAGGGTTGATCTTATGTAAAAATGTAAAAACTACCCAAGCCCATCAGCACTAGCCCAATGGACAATGGTCACAGTCCTAGTGGCTTAACTAGCTGCAATTTTTTATAATTTGTTTTGGATTCTCTTGGTAAATATAGTTTTTACTGGTTATAATGGAGATCTTTATCTTTTTGGGTCATGTGAAATGGAGATCGTATTTTGGTTTTTAAAACACTTTCTTTCTCTTCAGTTTTTATGATTAGTATTAGCCCCAAAGTTGACTTCTTTCAGAAGATAGGTTGAGAAGTAGATTGTTGATTTACTTTTTACATTGGCTATGAATCTGTGGCGACTTTATTAAGTTCTCAGGGCTATCCAGCCTAGTGCTGCAGTGATAGCATTTTACATCAGATTCTGG
CDS Sequence
  • >Glyma.03G196800.2
    ATGGATATATCAAATCCTGCAGCCTTAGTCAATGCGGAACTGTTGCACTTCTATGTTGGAAGAAGGGTAAGGATCGTGGTGCAGGTTGTGCGATCTGATGGTGGAGTTGTCATTGGAAAATCAACAGATGAGAAACAACTAGTTGTAAAAGGATCACCCCCACCTGCTGCTCCTCTTACAACTTTTGTTGAAGTTTATGGTCTTGTTAACAGTGATAAGTCAATTGGTGCTGAGATATGGACCAATTTTGGTGATGCCATCGATATGGATAGCTACAATAAGCTTTGTCAGCTCGCAAATGGAGAATTTAAACACTTGTTCCTGTGA
Protein Sequence
  • >Glyma.03G196800.2
    MDISNPAALVNAELLHFYVGRRVRIVVQVVRSDGGVVIGKSTDEKQLVVKGSPPPAAPLTTFVEVYGLVNSDKSIGAEIWTNFGDAIDMDSYNKLCQLANGEFKHLFL*