Gene Details:

Functional Descriptions:

  • A positive feedback loop mediated by CsERF31 initiates female cucumber flower development.
  • Here, we used gene expression analysis, protein–DNA interaction assays, and transgenic plants to study the function of a gynoecium-specific gene, ETHYLENE RESPONSE FACTOR31 (CsERF31), in female flower differentiation.
  • Knockdown of CsERF31 by RNAi causes defective bisexual flowers to replace female flowers. Ectopic expression of CsERF31 suppresses stamen development and promotes pistil development in male flowers, demonstrating that CsERF31 functions as a sex switch.
  • Our data confirm that CsERF31 represents the molecular link between female–male determination and female–bisexual determination, and provide mechanistic insight into how ethylene promotes female flowers, rather than bisexual flowers, in cucumber sex determination.
  • Constitutive expression of CsERF31 in cucumber promotes pistil development and arrests the stamen.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Csa_3G135120
    CCAAAACACACACACACACCACCTTCCTGCCTTCCTCCTCCGCTTCTACTACTTCTCAATCCATGGATTATTCTGCATTCATCTCCCCGCTTTCTGATTTCTCATCCGAATCATCTTTCGGTTCACCCGAATCCTCCTTCACCAATTTGGACCATAATTTTCTCCCTTTCAATGAAAATGACTCAGAGGAAATGCTTCTTTACGGCCTAATCTCCGAGGGCACATACGAATCATTCGATACAAGTATCGGAACCGTGCAAGTGAAGGAAGAGGAAGTCGATTCCATCGGAGAAGAAAGCCCGAAGAAAGAGAGGGCTTATAGAGGAGTTCGCCGCCGTCCATGGGGGAAATTTGCGGCGGAAATTAGAGATTCCACTAGACATGGTACAAGGGTATGGTTGGGAACTTTCGATAGTGCTGAAGCCGCCGCTTTGGCTTACGATCAAGCTGCCTTTTCGATGAGGGGCGCTGCCGCAATTCTCAATTTTCCTGTCGACAGAGTTAGAGAGTCTCTCAAAGAGATGAACGCCGGCAGTGGGGGCAGCGGTGATAGTTTAGCCGAAGACGGCGGCTCTCCGGTAGTGGCGTTAAAAAGAAAACACTCGATTAGAAGGAAAGCCATAGGTAAAAAGAGCAAAGAGAGAGATGTGAGGATTCAAACTGTGGTGGTTTTGGAAGATTTAGGGACAGAGTATTTGGAAGAACTTTTGGGGTCTTCTCAAAGTGATAGCCCTTCTTGTTCTTTCTAA
CDS Sequence
  • >Csa_3G135120
    ATGGATTATTCTGCATTCATCTCCCCGCTTTCTGATTTCTCATCCGAATCATCTTTCGGTTCACCCGAATCCTCCTTCACCAATTTGGACCATAATTTTCTCCCTTTCAATGAAAATGACTCAGAGGAAATGCTTCTTTACGGCCTAATCTCCGAGGGCACATACGAATCATTCGATACAAGTATCGGAACCGTGCAAGTGAAGGAAGAGGAAGTCGATTCCATCGGAGAAGAAAGCCCGAAGAAAGAGAGGGCTTATAGAGGAGTTCGCCGCCGTCCATGGGGGAAATTTGCGGCGGAAATTAGAGATTCCACTAGACATGGTACAAGGGTATGGTTGGGAACTTTCGATAGTGCTGAAGCCGCCGCTTTGGCTTACGATCAAGCTGCCTTTTCGATGAGGGGCGCTGCCGCAATTCTCAATTTTCCTGTCGACAGAGTTAGAGAGTCTCTCAAAGAGATGAACGCCGGCAGTGGGGGCAGCGGTGATAGTTTAGCCGAAGACGGCGGCTCTCCGGTAGTGGCGTTAAAAAGAAAACACTCGATTAGAAGGAAAGCCATAGGTAAAAAGAGCAAAGAGAGAGATGTGAGGATTCAAACTGTGGTGGTTTTGGAAGATTTAGGGACAGAGTATTTGGAAGAACTTTTGGGGTCTTCTCAAAGTGATAGCCCTTCTTGTTCTTTCTAA
Protein Sequence
  • >Csa_3G135120
    MDYSAFISPLSDFSSESSFGSPESSFTNLDHNFLPFNENDSEEMLLYGLISEGTYESFDTSIGTVQVKEEEVDSIGEESPKKERAYRGVRRRPWGKFAAEIRDSTRHGTRVWLGTFDSAEAAALAYDQAAFSMRGAAAILNFPVDRVRESLKEMNAGSGGSGDSLAEDGGSPVVALKRKHSIRRKAIGKKSKERDVRIQTVVVLEDLGTEYLEELLGSSQSDSPSCSF