Information report for CsHSFA1d
Gene Details
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Functional Descriptions
- Report that heat shock induces cold acclimation in cucumber seedlings, via a process involving the heat-shock transcription factor HSFA1d. CsHSFA1d expression was improved by both heat shock and cold treatment.
- Overexpressing CsHSFA1d displayed stronger tolerance for cold stress than the wild type, whereas CsHSFA1d knockdown lines obtained by RNA interference were more sensitive to cold stress.
- Both the overexpression of CsHSFA1d and heat-shock pre-treatment increased the endogenous jasmonic acid (JA) content in cucumber seedlings after cold treatment.
- CsHSFA1d improved JA biosynthesis by activating the expression of JA biosynthesis genes such as LIPOXYGENASE4 (CsLOX4) and 12-OXOPHYTODIENOATE REDUCTASE5 (CsOPR5) under cold temperatures.
- CsHSFA1d Promotes Drought Stress Tolerance by Increasing the Content of Raffinose Family Oligosaccharides and Scavenging Accumulated Reactive Oxygen Species in Cucumber.
- Identified that overexpression of CsHSFA1d could improve cold tolerance and the content of endogenous jasmonic acid in cucumber seedlings.
- CsHSFA1d also improves ROS-scavenging enzyme activity and gene expression indirectly to reduce drought-induced ROS overaccumulation.
- This study therefore offers a new gene target to improve drought stress tolerance in cucumber and revealed the underlying mechanism by which CsHSFA1d functions in the drought stress by increasing the content of RFOs and scavenging the excessive accumulation of ROS.
Functional Keywords
- transcription-factor , seedlings , stress , ja , tolerance , cold-tolerance , cold-stress , cold , stress-tolerance , jasmonic , jasmonic-acid , ja-biosynthesis , heat-tolerance , heat-stress
Literature and News
- Heat shock-induced cold acclimation in cucumber through CsHSFA1d-activated JA biosynthesis and signaling. DOI: 10.1111/tpj.15780 ; PMID: 35436390
- CsHSFA1d Promotes Drought Stress Tolerance by Increasing the Content of Raffinose Family Oligosaccharides and Scavenging Accumulated Reactive Oxygen Species in Cucumber. DOI: 10.1093/pcp/pcae023 ; PMID: 38564325
Gene Resources
- NCBI ID: 101207437
- UniProt accessions: A0A0A0L179
Homologs
- Cajanus cajan: C.cajan_25281
- Cicer arietinum: XP_004512503.1, XP_004512502.1
- Citrullus lanatus: Cla008825
- Citrus sinensis: orange1.1g010166m
- Cucumis melo: MELO3C013835P1
- Glycine max: Glyma.16G091800.1.p
- Gossypium arboreum: Cotton_A_33386_BGI-A2_v1.0
- Gossypium hirsutum: Gh_D12G2063, Gh_A03G1977
- Juglans regia: WALNUT_00026813-RA
- Manihot esculenta: Manes.02G225800.2.p, Manes.02G225800.1.p
- Medicago truncatula: Medtr5g029680.2, Medtr5g029680.1
- Populus trichocarpa: Potri.001G138900.1, Potri.003G095000.2, Potri.003G095000.1
- Ziziphus jujuba: XP_015897552.1
Sequences
cDNA Sequence
- >Csa_3G008920
ATGGACGGGACTGCTAATGGCTGTGATTCTGGATTGGCTTCCGGGAGTGGAAACTCTCATCCGACGGTTCCGGCTCCTATTACTAATTCTAGCGCGCCTCCACCGTTTCTGAGTAAGACATATGATATGGTGGATGACCCGGCTACCGATGCGGTTGTGTCCTGGAGCCCTACCAATAATAGTTTTGTGGTTTGGAATCCGCCTGAGTTCGCTAGGGATCTTTTGCCCAAATACTTCAAGCATAATAACTTCTCCAGTTTCGTCAGGCAGCTCAACACTTATGGATTCAGGAAAGTTGATCCAGACCGTTGGGAATTTGCTAACGAAGGCTTCTTAAGAGGTCAGAAACATCTGCTTAAGAGTATTACCCGGCGAAAACCTGTCCATGGGCAAAGTCAGCAACAACCACAGCAGTCTCACGGACAAAGTTCCTCAGTTGGAGCTTGTGTGGAAGTTGGTAAGTTTGGTTTGGAGGAAGAGGTTGAGAGGCTTAAAAGAGACAAAAATGTACTCATGCAGGAACTTGTGAGACTGAGGCAGCAGCAACAGACTACTGACAACCAGCTGCAAACTATGGTACAGCGTCTACAGGGGATGGAGCAGCGACAACAGCAGATGATGTCATTCCTTGCCAAGGCTGTGCAGAGCCCTGGCTTTTTGGCTCAGTTTGTGCAGCAGCAAAATGAAAGTACTAGACGCATAAGTGAGGCTAATAAAAAGCGAAGGCTAAAGCAGGATGGAATTGCCGAGTCTGATCATTCTCCTGTTCCAGATGGGCAAATTGTGAAGTATCAACCTCTCATGAATGAGGCAGCAAAAACAATGCTGAGGCAGATAATGAAAGTGGATACTTCACATTTGGAACCATCGAACCACAATACCGACAATTTTCTAATTCGTGATGGTTTGCAATCGCAGTGTGCAGCAATGGACAATGGGAACTCTTCCAGTTCTGTATCAGGCGTGACTCTTCAAGAGGTACCTCCAACCTCATCTTTTAATTCAGTTGCTTCTGGTGTTCCTCATGGCCCCTCAACAACCAAATCAGAAATCCAATCGTCACCTCAAGCTACAAATTCTGACAACATTTCAGCTTCTCCCTTTGCTCTTAACGCCGTGCGGGGTCCAGGGGCACGAGAAGCTTCTTCCTTATCAGTTTCTGAGACAGATGTAATAATGCCTGAGCTTTCCCATTTATCTGAAATGGTGTCTGAGAACATTCTTGATGTTCCTGAAGTGGATTATAGGGTGCCTGAGGCAGGAAATGGTGCATTTATAAGTCCGAATTTTTTGGATGCCAACGGAACTATTCCTATAGATATTGATAACATGTCCCCTGATGCTGACATCGATGCCTTGCTAGATAATTCCAACTTTTGGGATGATTTGCTTGTGCAAAGTCCATGTCAGGATGATGAGGTTGATTTTCTGGTTGGAGGAGGGTTACCAAAGACAAACGATATGCAACTGGCAGAAAATGCATGGGACAAATCTAAACATGTGGATAAACTTACAGAACAGATGGGCCTTCTCACTTCAGAAATTAAAAGGGTTTGA
CDS Sequence
- >Csa_3G008920
ATGGACGGGACTGCTAATGGCTGTGATTCTGGATTGGCTTCCGGGAGTGGAAACTCTCATCCGACGGTTCCGGCTCCTATTACTAATTCTAGCGCGCCTCCACCGTTTCTGAGTAAGACATATGATATGGTGGATGACCCGGCTACCGATGCGGTTGTGTCCTGGAGCCCTACCAATAATAGTTTTGTGGTTTGGAATCCGCCTGAGTTCGCTAGGGATCTTTTGCCCAAATACTTCAAGCATAATAACTTCTCCAGTTTCGTCAGGCAGCTCAACACTTATGGATTCAGGAAAGTTGATCCAGACCGTTGGGAATTTGCTAACGAAGGCTTCTTAAGAGGTCAGAAACATCTGCTTAAGAGTATTACCCGGCGAAAACCTGTCCATGGGCAAAGTCAGCAACAACCACAGCAGTCTCACGGACAAAGTTCCTCAGTTGGAGCTTGTGTGGAAGTTGGTAAGTTTGGTTTGGAGGAAGAGGTTGAGAGGCTTAAAAGAGACAAAAATGTACTCATGCAGGAACTTGTGAGACTGAGGCAGCAGCAACAGACTACTGACAACCAGCTGCAAACTATGGTACAGCGTCTACAGGGGATGGAGCAGCGACAACAGCAGATGATGTCATTCCTTGCCAAGGCTGTGCAGAGCCCTGGCTTTTTGGCTCAGTTTGTGCAGCAGCAAAATGAAAGTACTAGACGCATAAGTGAGGCTAATAAAAAGCGAAGGCTAAAGCAGGATGGAATTGCCGAGTCTGATCATTCTCCTGTTCCAGATGGGCAAATTGTGAAGTATCAACCTCTCATGAATGAGGCAGCAAAAACAATGCTGAGGCAGATAATGAAAGTGGATACTTCACATTTGGAACCATCGAACCACAATACCGACAATTTTCTAATTCGTGATGGTTTGCAATCGCAGTGTGCAGCAATGGACAATGGGAACTCTTCCAGTTCTGTATCAGGCGTGACTCTTCAAGAGGTACCTCCAACCTCATCTTTTAATTCAGTTGCTTCTGGTGTTCCTCATGGCCCCTCAACAACCAAATCAGAAATCCAATCGTCACCTCAAGCTACAAATTCTGACAACATTTCAGCTTCTCCCTTTGCTCTTAACGCCGTGCGGGGTCCAGGGGCACGAGAAGCTTCTTCCTTATCAGTTTCTGAGACAGATGTAATAATGCCTGAGCTTTCCCATTTATCTGAAATGGTGTCTGAGAACATTCTTGATGTTCCTGAAGTGGATTATAGGGTGCCTGAGGCAGGAAATGGTGCATTTATAAGTCCGAATTTTTTGGATGCCAACGGAACTATTCCTATAGATATTGATAACATGTCCCCTGATGCTGACATCGATGCCTTGCTAGATAATTCCAACTTTTGGGATGATTTGCTTGTGCAAAGTCCATGTCAGGATGATGAGGTTGATTTTCTGGTTGGAGGAGGGTTACCAAAGACAAACGATATGCAACTGGCAGAAAATGCATGGGACAAATCTAAACATGTGGATAAACTTACAGAACAGATGGGCCTTCTCACTTCAGAAATTAAAAGGGTTTGA
Protein Sequence
- >Csa_3G008920
MDGTANGCDSGLASGSGNSHPTVPAPITNSSAPPPFLSKTYDMVDDPATDAVVSWSPTNNSFVVWNPPEFARDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSITRRKPVHGQSQQQPQQSHGQSSSVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQTTDNQLQTMVQRLQGMEQRQQQMMSFLAKAVQSPGFLAQFVQQQNESTRRISEANKKRRLKQDGIAESDHSPVPDGQIVKYQPLMNEAAKTMLRQIMKVDTSHLEPSNHNTDNFLIRDGLQSQCAAMDNGNSSSSVSGVTLQEVPPTSSFNSVASGVPHGPSTTKSEIQSSPQATNSDNISASPFALNAVRGPGAREASSLSVSETDVIMPELSHLSEMVSENILDVPEVDYRVPEAGNGAFISPNFLDANGTIPIDIDNMSPDADIDALLDNSNFWDDLLVQSPCQDDEVDFLVGGGLPKTNDMQLAENAWDKSKHVDKLTEQMGLLTSEIKRV