Gene Details:

Functional Descriptions:

  • CsHMA3 was predominantly expressed in roots and up-regulated by Pb, Zn and Cd excess, whereas the CsHMA4 transcript was most abundant in roots and flowers of cucumber plants, and elevated under Pb and Zn excess.
  • Expression of CsHMA3 in Saccharomyces cerevisiae enhanced yeast tolerance to Cd and Pb, whereas CsHMA4 conferred increased resistance of yeast cells to Cd and Zn.
  • Immunostaining with specific antibodies raised against cucumber proteins revealed tonoplast localization of CsHMA3 and plasma membrane localization of CsHMA4 in cucumber root cells.
  • Altogether, the results suggest an important role of CsHMA3 and CsHMA4 in Cd and Pb detoxification and Zn homeostasis in cucumber cells.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Csa_2G060450
    CTACCTCTTACACAACCATTTTTTTTTTGTAAGCTTTGCTTCACATTCTCTTCTGTTCCAAATACTTTTCTACTTATATATGAATTTGACAGTCATTTTGATTTTTTTTTTGGGTCTTGGGCTTCCCAAAATCTTGCACAAAATGTTATTTCAATTGTGTTAGAACTTAGATGCTTATTTCTTTTACATTCCCATTAACTATTTTTCTTTTTTAACAGTATCCAAAATCAGTGCTCACATCATTTCTTGTTTTTCAATACTATTTCTCTCTCTGTTCTCTCAACCGATTCAACTGTTTTTTAATACTATAGAAAGTGATTCAAACTGTTTACTAGTTTTGATGAAGTGAGTTTGGTTTAATGACATTTTTTTAATAATTTCTAGAAATGACGTGCATATCAAATGTCATATTGAACGATCTCTACCTAAGTTTCACCATGCTAGCAGCCTTTTAAATACATTGTAAAATCTATTTCATTGTTGATATTATGTTTTGTTGTTCAAATAAAGAAAGCTTTCTCTCTCTCATTTGCACAGTGGCCATAGATCACTTCCTTCTTTAATTGTCCTCTTCCTCTTCACTTTTTCTTTTATACAAAATTTAACTATACATTTGACAAATTTTTATTGGGTAACGATCTGAGCTGCACAAAAGTAGGTGAAGCCGATGCAGCATCTAAGTTTTTTTCTCGTTTTATTATAATTTTCTTCTTCAATTGAGAGTCCACTTTAATTCATGATGATTGACATGTTATTACTAATTCTTGCCAATAAAAAACATGCAACTTTTGAAACTACGTTGACCCTAGTATTTAATCATTTATTTTCAATTTATCAAACAGTAACTAGACATGATATATATGAGTTATTCATTCCATTTGTCAATTAATTATTTAAAAACGATGAAACATGTATGTTTATTTTGCAGAAAAATTAATTTGCGAGGAAGATAATATAATTAATCCGTCATGAGTGAGGAGGCAACCGAGAAGATGAATAAGAAAGTAATAAAAGAAAACAAAAAAATGGAAAGAAGCCATTTTGATGTGTTGGGAATTTGCTGTTCTTCAGAAATCCCTTTGATTGAAAACATCCTCAAACCCCTCCAAGGAATCAAACAAATCACTGTCATTGTCCCTACAAGAACACTCATTGTTGTTCATGATTCGCTTCTCATTTCCCAACTTCAAATTGTGAAAGCTCTAAATGAAGCAAGATTGGAGGCAAATATACAATTAAAAGGAAAGGGAATTATGAAGAAGAAATGGCCAAGTCCTTATGCAATAGCCAGTGGGTTGCTTCTAACTGCGTCGTTTTTGAAGTATGTCTACCACCCATTGCGGTGGCTTGCCGTTGCAGCCGTTGCCGCCGGCATCTTTCCCATTCTTCTAAAAGCCATTTCTGCCATTCGCCACCTCAGAGTTGATGTCAACATTCTTGCTATTATTGCAGTTGTGGGGACAATTGCAATGGATGATTATATGGAAGCTGGGAGTATAGTGTTTTTGTTTTCCATTGCCGAATGGCTTGAGTCAAGAGCAAGCCACAAGGCGAATGGTGCAATGTGTTCACTAATGAGGTTAGCTCCCCAGAAAGCCACAATAGCTGAAAGTGGAGAGGTTGTGGATGTAAGAGATGTGAAATTGAAGAGTGTGTTAGGTGTGAAAGCTGGAGAAGTTATTCCGATTGATGGGATTGTTGTTGAAGGCAATTGTGAAGTTGATGAGAAAACTTTGAGTGGAGAAACTTTCCCTGTTACCAAACAAAAAGATTCTCTTGTTTGGGCTGGTACCATCAACTTAAATGGTTACATAAGTGTTCAAACAACAGTTGTAGCTGAGGATTGTGTGGTAGCAAAAATGGCTGAGCTTGTAGAAGAAGCTCAAAATAACAAATCAAAAACTCAAACATTCATTGATGAATGTGCCAAATATTACACCCCAGCTGTTGTTATCATCTCAGCTTGTCTAGCAGCCATTCCAGCTGCATTGCGAGTTCATAATCTCAGGCATTGGCTTCACTTAGCTTTGGTGGTTTTAGTGAGTGCATGTCCTTGTGCCTTAATCCTCTCCACCCCGGTCGCCGCATTTTGTGCTCTTACGAAGGCAGCCATGGCCGGAGTTTTGATCAAAGGCGGCAACCACCTTGAAGTTCTTGCAAAGATTAAGGTTATGGCCTTTGATAAAACTGGCACGATCACTAGAGGTGAATTTGTTGTAACACATTTTCAAGCTCTAAGAGATGATATTAACTTCCACACCTTGCTCCAATGGGTTTCAAGCATTGAGAGCAAATCAAGCCATCCAATGGCAACTGCCCTTGTCAACTACGGAAAGCTGCATTCTATCGATCTTAAGCCGGAAAACGTAGAGGAATTTGAAAATTTTCTAGGAGAAGGTGTTCGTGGGAAGATAGATGGTAATGATATTTACATTGGAAGCAAAAAAATTGCTGCCAGAGCTGGCTACGACATACCAGTTTCTTCAAAATTGAACAACTTTGACGATGAAACGAGACAAGAGCAAACCCTTGGATATGTATTTTGTGGGGGGATGATGATTGGATCTTTCGGTTTATTGGATTCGTGTCGTTCCGGAGTTAAAGAGGCAATCGAGGAGATCAAAAGTTTTGGAATTAAAACTGCTATGCTCACAGGAGATTGTAGGGCAGCTGCCATGCATGTCCAAGAACAGCTCGGGAATACGCTTGACGTAATCCATTCAGAACTTCTACCAAAGGAGAAGGCAAATATCATAAAAGAATTTAAAAATAATGATGGAGCAATTGCCATGGTTGGAGATGGCTTAAATGATACCCCAGCATTAGCTACCGCTGATATTGGCATGTCAATGGGTATTTCAGGTTCAGCATTGGCCACTGAAACTGGGAATGTGATTTTAATGTCAAATGACATGAGAAAGATTCCAAAAGCCATCAAACTAGCAAAGACATTCCACACAAAAGTAGTTCAAAATGTCATTTTGTCAATCGGTACAAAGACTGCCATCCTAGGCTTGGCATTTGCTGGACATCCCCTCATTTGGGCAGCCGTTCTTGCTGATGTCGGTACTTGTCTATTGGATCACAAGATGCAACTTTCACAAGATCACAATAAAGAAACATGTGGAGTACTGAACCAAGAGAAGAATAATCATGAATGTGGAGAGCATGAGTGTGAGGAAACTAATGTGCATCATAAAAAAGAAGATAAATTTCATCATAACTATTCAAATCAATGTGAGAAAACCCCACTTGAGAGAGAGATTAGGGGAAATTCTTCAAAGAGAGTGGGTAAATCAGATTGTAATTGCCACTCACATCATGTTACAATTGACATTCATGAAAGTAATGAGTGTGAGAGAGTAGATCATAATTAGCGAAGTCTCTTAATTATGAGGAACTAATGGTTGGTATGGAGTTTAAAGGGAAAGAGATAATACAAATGTATGAAGAAATGCAAAAAAGGCC
CDS Sequence
  • >Csa_2G060450
    ATGAGTGAGGAGGCAACCGAGAAGATGAATAAGAAAGTAATAAAAGAAAACAAAAAAATGGAAAGAAGCCATTTTGATGTGTTGGGAATTTGCTGTTCTTCAGAAATCCCTTTGATTGAAAACATCCTCAAACCCCTCCAAGGAATCAAACAAATCACTGTCATTGTCCCTACAAGAACACTCATTGTTGTTCATGATTCGCTTCTCATTTCCCAACTTCAAATTGTGAAAGCTCTAAATGAAGCAAGATTGGAGGCAAATATACAATTAAAAGGAAAGGGAATTATGAAGAAGAAATGGCCAAGTCCTTATGCAATAGCCAGTGGGTTGCTTCTAACTGCGTCGTTTTTGAAGTATGTCTACCACCCATTGCGGTGGCTTGCCGTTGCAGCCGTTGCCGCCGGCATCTTTCCCATTCTTCTAAAAGCCATTTCTGCCATTCGCCACCTCAGAGTTGATGTCAACATTCTTGCTATTATTGCAGTTGTGGGGACAATTGCAATGGATGATTATATGGAAGCTGGGAGTATAGTGTTTTTGTTTTCCATTGCCGAATGGCTTGAGTCAAGAGCAAGCCACAAGGCGAATGGTGCAATGTGTTCACTAATGAGGTTAGCTCCCCAGAAAGCCACAATAGCTGAAAGTGGAGAGGTTGTGGATGTAAGAGATGTGAAATTGAAGAGTGTGTTAGGTGTGAAAGCTGGAGAAGTTATTCCGATTGATGGGATTGTTGTTGAAGGCAATTGTGAAGTTGATGAGAAAACTTTGAGTGGAGAAACTTTCCCTGTTACCAAACAAAAAGATTCTCTTGTTTGGGCTGGTACCATCAACTTAAATGGTTACATAAGTGTTCAAACAACAGTTGTAGCTGAGGATTGTGTGGTAGCAAAAATGGCTGAGCTTGTAGAAGAAGCTCAAAATAACAAATCAAAAACTCAAACATTCATTGATGAATGTGCCAAATATTACACCCCAGCTGTTGTTATCATCTCAGCTTGTCTAGCAGCCATTCCAGCTGCATTGCGAGTTCATAATCTCAGGCATTGGCTTCACTTAGCTTTGGTGGTTTTAGTGAGTGCATGTCCTTGTGCCTTAATCCTCTCCACCCCGGTCGCCGCATTTTGTGCTCTTACGAAGGCAGCCATGGCCGGAGTTTTGATCAAAGGCGGCAACCACCTTGAAGTTCTTGCAAAGATTAAGGTTATGGCCTTTGATAAAACTGGCACGATCACTAGAGGTGAATTTGTTGTAACACATTTTCAAGCTCTAAGAGATGATATTAACTTCCACACCTTGCTCCAATGGGTTTCAAGCATTGAGAGCAAATCAAGCCATCCAATGGCAACTGCCCTTGTCAACTACGGAAAGCTGCATTCTATCGATCTTAAGCCGGAAAACGTAGAGGAATTTGAAAATTTTCTAGGAGAAGGTGTTCGTGGGAAGATAGATGGTAATGATATTTACATTGGAAGCAAAAAAATTGCTGCCAGAGCTGGCTACGACATACCAGTTTCTTCAAAATTGAACAACTTTGACGATGAAACGAGACAAGAGCAAACCCTTGGATATGTATTTTGTGGGGGGATGATGATTGGATCTTTCGGTTTATTGGATTCGTGTCGTTCCGGAGTTAAAGAGGCAATCGAGGAGATCAAAAGTTTTGGAATTAAAACTGCTATGCTCACAGGAGATTGTAGGGCAGCTGCCATGCATGTCCAAGAACAGCTCGGGAATACGCTTGACGTAATCCATTCAGAACTTCTACCAAAGGAGAAGGCAAATATCATAAAAGAATTTAAAAATAATGATGGAGCAATTGCCATGGTTGGAGATGGCTTAAATGATACCCCAGCATTAGCTACCGCTGATATTGGCATGTCAATGGGTATTTCAGGTTCAGCATTGGCCACTGAAACTGGGAATGTGATTTTAATGTCAAATGACATGAGAAAGATTCCAAAAGCCATCAAACTAGCAAAGACATTCCACACAAAAGTAGTTCAAAATGTCATTTTGTCAATCGGTACAAAGACTGCCATCCTAGGCTTGGCATTTGCTGGACATCCCCTCATTTGGGCAGCCGTTCTTGCTGATGTCGGTACTTGTCTATTGGATCACAAGATGCAACTTTCACAAGATCACAATAAAGAAACATGTGGAGTACTGAACCAAGAGAAGAATAATCATGAATGTGGAGAGCATGAGTGTGAGGAAACTAATGTGCATCATAAAAAAGAAGATAAATTTCATCATAACTATTCAAATCAATGTGAGAAAACCCCACTTGAGAGAGAGATTAGGGGAAATTCTTCAAAGAGAGTGGGTAAATCAGATTGTAATTGCCACTCACATCATGTTACAATTGACATTCATGAAAGTAATGAGTGTGAGAGAGTAGATCATAATTAG
Protein Sequence
  • >Csa_2G060450
    MSEEATEKMNKKVIKENKKMERSHFDVLGICCSSEIPLIENILKPLQGIKQITVIVPTRTLIVVHDSLLISQLQIVKALNEARLEANIQLKGKGIMKKKWPSPYAIASGLLLTASFLKYVYHPLRWLAVAAVAAGIFPILLKAISAIRHLRVDVNILAIIAVVGTIAMDDYMEAGSIVFLFSIAEWLESRASHKANGAMCSLMRLAPQKATIAESGEVVDVRDVKLKSVLGVKAGEVIPIDGIVVEGNCEVDEKTLSGETFPVTKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQTFIDECAKYYTPAVVIISACLAAIPAALRVHNLRHWLHLALVVLVSACPCALILSTPVAAFCALTKAAMAGVLIKGGNHLEVLAKIKVMAFDKTGTITRGEFVVTHFQALRDDINFHTLLQWVSSIESKSSHPMATALVNYGKLHSIDLKPENVEEFENFLGEGVRGKIDGNDIYIGSKKIAARAGYDIPVSSKLNNFDDETRQEQTLGYVFCGGMMIGSFGLLDSCRSGVKEAIEEIKSFGIKTAMLTGDCRAAAMHVQEQLGNTLDVIHSELLPKEKANIIKEFKNNDGAIAMVGDGLNDTPALATADIGMSMGISGSALATETGNVILMSNDMRKIPKAIKLAKTFHTKVVQNVILSIGTKTAILGLAFAGHPLIWAAVLADVGTCLLDHKMQLSQDHNKETCGVLNQEKNNHECGEHECEETNVHHKKEDKFHHNYSNQCEKTPLEREIRGNSSKRVGKSDCNCHSHHVTIDIHESNECERVDHN