Gene Details:

Functional Descriptions:

  • Cucumber malate decarboxylase, CsNADP-ME2, functions in the balance of carbon and amino acid metabolism in fruit.
  • Histochemical localization revealed that CsNADP-ME2 was mainly located in the exocarp and vascular bundle system of fruit.
  • Radiotracer and gas-exchange analysis indicated that overexpression of CsNADP-ME2 could promote carbon flux into soluble sugars and starch in fruits.
  • Further studies combined with metabolic profiling revealed that the downregulation of CsNADP-ME2 in RNA interference (RNAi) lines caused the accumulation of its substrate, malate, in the exocarp.
  • The opposite effect was found in CsNADP-ME2-overexpressing lines, suggesting that there may be a continuous bottom-up feedback regulation of glycolysis in cucumber fruits.
  • Overall, our studies indicate that CsNADP-ME2 may play potential roles in both central carbon reactions and amino acid metabolism in cucumber fruits.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Csa_1G574870
    TATCTTTCGCCAAATTTGACTTTGCCTTTTCTCCTCGTCAATGGTGGCAAGTGGGTTTGGTTATTTAATGGACGCCCAAGTCTTCCCAATTCGTATTGTGTTTATTTTTTGAGGAAGATTTGTTTTGCCACTCTTCTTTTTGTGGATTGCTTCAATTTTGAGTTTAGTGATCTGGGATGGAGAGTACTTTGAAGGAGATCGGTGATGGTGGTTCTGTGTTGGATTTGGACCCCAAAGCAACTGTTGGTGGTGGTGTTGAGGATATATATGGAGAAGACTGTGCTACAGAGGAACAGCTTGTCACTCCGTGGACTTTCTCAGTCGCGAGTGGCTATTCGTTGTTGAGGGATCCGCATCATAACAAGGGGTTGGCATTTACAGAAAAGGAAAGAGATGCTCATTATTTGCGTGGGCTTTTGCCACCTGCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAGAACATCAGGCAGTATCAACTTCCACTACAAAAATTTATTGCCATGATGGAACTTCAGGAAAGAAATGAAAGGCTTTTTTACAAACTTCTTGTTGACAATGTCGAAGAACTGCTTCCTGTTGTCTACACTCCCACAGTTGGTGAAGCTTGTCAGAAGTACGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATTAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGGAGCATCCAAGTAATTGTGGTGACTGATGGTGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAGGGAATGGGAATTCCAGTTGGAAAACTTTCTTTATACACCGCACTTGGAGGAGTTCGTCCTTCTGCATGTTTGCCTATTACAATTGATGTTGGGACAAACAACGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAGAGAAGAGCAACCGGAGAGGAATACTATGAACTTCTAGATGAGTTTATGACTGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTGATTCAGTTTGAAGATTTTGCAAACCACAATGCTTTTGAGCTACTTGCCAAGTATCGCACAACTCATCTAGTCTTCAATGATGACATTCAGGGGACAGCGGCTGTCGTTCTTGCTGGTGCTGTTTCCGCCCTCAAACTCATTGGTGGTACCTTGGCCGATCACACGTTCTTGTTCCTTGGTGCTGGGGAAGCTGGAACTGGTATTGCAGAGCTTATAGCCCTTGAAGTATCTAAACAGACAAAAGCTCCAGTTGAGGAGACCCGCAAGAAGATCTGGCTTGTCGACTCGAAGGGATTAATTGTCCACTCGCGTAAGGATTCACTTCAACACTTCAAGAAGCCATGGGCTCACGAGCATGAACCTGTCAAGGATTTATTAAGTGCTGTGAAGGCAATTAAGCCAACAGTTCTGATTGGATCATCTGGAGTGGGAAGGACTTTTACAAAAGAAGTCGTCGAGGCTGTATCCTCCATCAACGAGAAACCTCTTATTATGGCTCTTTCCAACCCAACGTCACAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTGAGGGCCGTGCAATCTTTGCCAGTGGAAGTCCATTCGATCCATTTGAATATAACGGGAAAACCTTTGTCCCTGGCCAGTCCAACAATGCCTACATCTTCCCTGGGTTCGGTCTGGGTGTCGTAATTTCTGGAGCAATTCGTGTCCACGACGACATGCTTCTGGCTGCTTCGGAAGCATTGGCTGCCCAAGTCTCCGAGGAGAACTACGACAAGGGATTGATCTACCCGCCCTTTACTAATATCCGAAAAATCTCGGCAAACATTGCAGCTAATGTGGCTGCCAAAGCATATGAACTTGGTTTGGCAACCCGTCTCCCACGCCCTGCAGATCTAGTCAAGTATGCTGAGAGCTGCATGTACAGTCCCGTCTACCGAACCTACCGATAAATGTAACAAGGTATCGTTGTGGTGGTCTGCATTTTAGAGCTTTTTATAGGAGCTCTGTAATATCCTTATACTGTGTGCTTTAAGAGAGAGCTTGAAGTCTTCCCAAAATCTATCACTTGTAACGATGTAATGTTGTAATGTTTTTTCCTTCTGAAGTTTTTTTGTTAATGGAATAAGTTTTTGTATCAATTTCCAGCTGCTTTAGTTTCTTCTCTTTGTGAGAGTAGTAGATTTTTCACCAGCA
CDS Sequence
  • >Csa_1G574870
    ATGGAGAGTACTTTGAAGGAGATCGGTGATGGTGGTTCTGTGTTGGATTTGGACCCCAAAGCAACTGTTGGTGGTGGTGTTGAGGATATATATGGAGAAGACTGTGCTACAGAGGAACAGCTTGTCACTCCGTGGACTTTCTCAGTCGCGAGTGGCTATTCGTTGTTGAGGGATCCGCATCATAACAAGGGGTTGGCATTTACAGAAAAGGAAAGAGATGCTCATTATTTGCGTGGGCTTTTGCCACCTGCCATTGTTACTCAACAACTTCAGGAGAAGAAGTTGATGCAGAACATCAGGCAGTATCAACTTCCACTACAAAAATTTATTGCCATGATGGAACTTCAGGAAAGAAATGAAAGGCTTTTTTACAAACTTCTTGTTGACAATGTCGAAGAACTGCTTCCTGTTGTCTACACTCCCACAGTTGGTGAAGCTTGTCAGAAGTACGGAAGCATCTTCAGGCGTCCTCAGGGTCTTTACATTAGTTTGAAAGAGAAGGGTAAGATTCTTGAAGTATTGAAGAATTGGCCCCAAAGGAGCATCCAAGTAATTGTGGTGACTGATGGTGAGCGTATTTTGGGTCTTGGTGATCTTGGTTGTCAGGGAATGGGAATTCCAGTTGGAAAACTTTCTTTATACACCGCACTTGGAGGAGTTCGTCCTTCTGCATGTTTGCCTATTACAATTGATGTTGGGACAAACAACGAGAAATTGTTGAATGATGAATTCTACATTGGGCTTAAACAGAGAAGAGCAACCGGAGAGGAATACTATGAACTTCTAGATGAGTTTATGACTGCTGTTAAGCAGAATTATGGGGAAAAAGTTCTGATTCAGTTTGAAGATTTTGCAAACCACAATGCTTTTGAGCTACTTGCCAAGTATCGCACAACTCATCTAGTCTTCAATGATGACATTCAGGGGACAGCGGCTGTCGTTCTTGCTGGTGCTGTTTCCGCCCTCAAACTCATTGGTGGTACCTTGGCCGATCACACGTTCTTGTTCCTTGGTGCTGGGGAAGCTGGAACTGGTATTGCAGAGCTTATAGCCCTTGAAGTATCTAAACAGACAAAAGCTCCAGTTGAGGAGACCCGCAAGAAGATCTGGCTTGTCGACTCGAAGGGATTAATTGTCCACTCGCGTAAGGATTCACTTCAACACTTCAAGAAGCCATGGGCTCACGAGCATGAACCTGTCAAGGATTTATTAAGTGCTGTGAAGGCAATTAAGCCAACAGTTCTGATTGGATCATCTGGAGTGGGAAGGACTTTTACAAAAGAAGTCGTCGAGGCTGTATCCTCCATCAACGAGAAACCTCTTATTATGGCTCTTTCCAACCCAACGTCACAATCTGAATGCACTGCTGAAGAGGCTTACACTTGGAGTGAGGGCCGTGCAATCTTTGCCAGTGGAAGTCCATTCGATCCATTTGAATATAACGGGAAAACCTTTGTCCCTGGCCAGTCCAACAATGCCTACATCTTCCCTGGGTTCGGTCTGGGTGTCGTAATTTCTGGAGCAATTCGTGTCCACGACGACATGCTTCTGGCTGCTTCGGAAGCATTGGCTGCCCAAGTCTCCGAGGAGAACTACGACAAGGGATTGATCTACCCGCCCTTTACTAATATCCGAAAAATCTCGGCAAACATTGCAGCTAATGTGGCTGCCAAAGCATATGAACTTGGTTTGGCAACCCGTCTCCCACGCCCTGCAGATCTAGTCAAGTATGCTGAGAGCTGCATGTACAGTCCCGTCTACCGAACCTACCGATAA
Protein Sequence
  • >Csa_1G574870
    MESTLKEIGDGGSVLDLDPKATVGGGVEDIYGEDCATEEQLVTPWTFSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPAIVTQQLQEKKLMQNIRQYQLPLQKFIAMMELQERNERLFYKLLVDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPQRSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGEEYYELLDEFMTAVKQNYGEKVLIQFEDFANHNAFELLAKYRTTHLVFNDDIQGTAAVVLAGAVSALKLIGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVEETRKKIWLVDSKGLIVHSRKDSLQHFKKPWAHEHEPVKDLLSAVKAIKPTVLIGSSGVGRTFTKEVVEAVSSINEKPLIMALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPFEYNGKTFVPGQSNNAYIFPGFGLGVVISGAIRVHDDMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIAANVAAKAYELGLATRLPRPADLVKYAESCMYSPVYRTYR