Gene Details:
- Gene ID: evm.TU.scaffold_11269.108-Cmo
- Gene Symbol: DgTIL1
- Gene Name: temperature-induced lipocalin-1-like
- Genome: Chrysanthemum morifolium genome
- Species: Chrysanthemum morifolium
Functional Descriptions:
- Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum.
- DgTIL1 transgenic chrysanthemum, thus promoting the cold resistance of chrysanthemum.
- Overexpression of DgTIL1 improves the cold resistance of chrysanthemum.
- Identified a temperature-induced lipocalin-1-like gene (DgTIL1) from chrysanthemum and showed that it was notably induced in response to cold stress.
- Overexpression of DgTIL1 enhanced cold tolerance in transgenic chrysanthemum. Ubiquitin membrane yeast two-hybrid (MYTH) system and bimolecular fluorescence complementation (BIFC) assays showed that DgTIL1 interacts with a nonspecific lipid transfer protein (DgnsLTP), which can promote peroxidase (POD) gene expression and POD activity to reduce the accumulation of reactive oxygen species (ROS) and improve resistance to cold stress in DgnsLTP transgenic chrysanthemum.
- In addition, we found that DgTIL1 was lysine crotonylated at K72 in response to low temperature in chrysanthemum.
- Inhibition of DgnsLTP degradation by lysine crotonylation of DgTIL1 further enhanced POD expression and POD activity, reduced the accumulation of ROS under cold stress in DgTIL1 transgenic chrysanthemum, thus promoting the cold resistance of chrysanthemum.
Function-related keywords:
- temperature , resistance , stress , tolerance , cold-tolerance , cold-stress , cold , stress-tolerance , ubiquitin , reactive-oxygen-species , stress-response , temperature-response , protein
Literature:
- Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum. DOI: 10.1111/pbi.13533 ; PMID: 33368971
Related News:
Gene Resources:
- NCBI ID: MT219513
- UniProt accessions: A0A7L7S218
Orthologs:
Sequences:
cDNA Sequence
- >evm.TU.scaffold_11269.108-Cmo
ATGGCAAAGAAAGAAATGGAAGTAGTAAAGGGTGTTGATCTAGCAAGGTACATGGGGAGATGGTATGAAATAGCTTCCTTTCCATCAAGGTTTCAGCCCAAAAATGGGACTAACACAAGGGCTACTTACACTTTGAAAGATGATGGTACCGTTAATGTGTTGAATGAAACATGGACTGATGGTAAGAGGGGATATATTGAAGGAACTGCTTATAAGGCTGATCCTAAAAGTGATGAGGCTAAATTGAAGGTTAAGTTTTGGGTGCCACCGTTTTTGCCTATTATTCCTGTGACTGGTGATTATTGGGTTTTGTATCTTGATGATGATTATCAGTATGCTTTGATTGGTCAGCCTTCAAGGAGTTATCTTTGGATATTGTGCAGGCAAAACCATCTTGATGATGAGATTTACAATCGGCTGGTTGAGAAAGCCAAAGAAGAAGGCTATGACGTCACCAAACTTAAGAAGACAACACACACAAACCCACCGCCTGAGACTGACGATGCACCTGCAGACACCAAGGGTGTGTGGTATATCAAGTCACTCTTTGGTAAATAG
CDS Sequence
- >evm.TU.scaffold_11269.108-Cmo
ATGGCAAAGAAAGAAATGGAAGTAGTAAAGGGTGTTGATCTAGCAAGGTACATGGGGAGATGGTATGAAATAGCTTCCTTTCCATCAAGGTTTCAGCCCAAAAATGGGACTAACACAAGGGCTACTTACACTTTGAAAGATGATGGTACCGTTAATGTGTTGAATGAAACATGGACTGATGGTAAGAGGGGATATATTGAAGGAACTGCTTATAAGGCTGATCCTAAAAGTGATGAGGCTAAATTGAAGGTTAAGTTTTGGGTGCCACCGTTTTTGCCTATTATTCCTGTGACTGGTGATTATTGGGTTTTGTATCTTGATGATGATTATCAGTATGCTTTGATTGGTCAGCCTTCAAGGAGTTATCTTTGGATATTGTGCAGGCAAAACCATCTTGATGATGAGATTTACAATCGGCTGGTTGAGAAAGCCAAAGAAGAAGGCTATGACGTCACCAAACTTAAGAAGACAACACACACAAACCCACCGCCTGAGACTGACGATGCACCTGCAGACACCAAGGGTGTGTGGTATATCAAGTCACTCTTTGGTAAATAG
Protein Sequence
- >evm.TU.scaffold_11269.108-Cmo
MAKKEMEVVKGVDLARYMGRWYEIASFPSRFQPKNGTNTRATYTLKDDGTVNVLNETWTDGKRGYIEGTAYKADPKSDEAKLKVKFWVPPFLPIIPVTGDYWVLYLDDDYQYALIGQPSRSYLWILCRQNHLDDEIYNRLVEKAKEEGYDVTKLKKTTHTNPPPETDDAPADTKGVWYIKSLFGK