Gene Details:

  • Gene ID:
  • Gene Symbol: CsDFRc
  • Gene Name: dihydroflavonol 4-reductase
  • Genome:
  • Species: Camellia sinensis

Functional Descriptions:

  • Enzyme activity analyses revealed that the two recombinant proteins CsDFRa and CsDFRc exhibited DFR activity, converting dihydroflavonols into leucoanthocyanins in vitro, but CsDFRb1 and CsDFRb3 did not.
  • CsDFRa and CsDFRc not only restored the purple petiole phenotype but also recovered the seed coat color of the tt3 mutant when compared with tt3 and CsDFRb1 transgenic linesx.
  • CsDFRa and CsDFRc overexpression in AtDFR mutants (tt3) revealed that CsDFRs are involved in the biosynthesis of anthocyanins and PAs, as CsDFRa and CsDFRc restored not only the purple petiole phenotype but also the seed coat color.
  • Enzymatic assays indicated that CsDFRa and CsDFRc exhibited a higher affinity for DHQ and DHK, respectively, whereas CsDFRb1N120S and CsDFRb1C126T exhibited a higher affinity for DHM.
  • The expression levels of other genes such as CsDFRb1, CsDFRb3, and CsDFRc were highest in the root and lowest in the mature leaf and stem, consistent with the concentration of PA and indicating the involvement of these three genes in PA biosynthesis in the tea plant.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
CDS Sequence
Protein Sequence