Overexpression of Sinapine Esterase BnSCE3 in Oilseed Rape Seeds Triggers Global Changes in Seed Metabolism.
During early stages of seedling development, a sinapine esterase (BnSCE3) hydrolyzes sinapine, releasing choline and sinapate.
Seeds of BnSCE3-overexpressing lines were increased in size by about 16%. This was accompanied by increases in seed weight by up to 60% and in seed moisture by about 26%.
Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism.