Gene Details:
- Gene ID: BnaA07g12390D
- Gene Symbol: BnMAN7A07
- Gene Name: MANNANASE7
- Genome: B. napus reference genome Darmor v4.1
- Species: Brassica napus
Functional Descriptions:
- An AtMAN7 homolog from rapeseed, and demonstrate its function in the dehiscence of rapeseed siliques.
- BnMAN7A07 was expressed in both vegetative and reproductive organs and significantly highly expressed in leaves, flowers and siliques where the abscission or dehiscence process occurs.
- The expression of hemicellulase gene BnMAN7A07, which could provide an available genetic resource for breeding practice in rapeseed which is beneficial to mechanized harvest.
- The biological activity of the BnMAN7A07 protein isolated and purified through prokaryotic expression system was verified to catalyse the decomposition of xylan into xylose.
- Our results demonstrate that dehiscence-resistance can be manipulated by altering the expression of hemicellulase gene BnMAN7A07, which could provide an available genetic resource for breeding practice in rapeseed which is beneficial to mechanized harvest.
- The tissue-specific expression profile of BnMAN7A07 may provide an expression support to the potential biological functions associated with the silique dehiscence.
Function-related keywords:
Literature:
- Down-regulation of MANNANASE7 gene in Brassica napus L. enhances silique dehiscence-resistance. DOI: 10.1007/s00299-020-02638-5 ; PMID: 33392730
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >BnaA07g12390D
GTTTCGAGCAGTAAACGAGACGACGGCGTTTAGAGTACGTGATTGTCGTCAACGACTCAACGTCGTCGGGTCTGCTCCTTCTTCTTCTTCACTCTCTAGACTGGTGATCCATTCTATACCCGTGAATAAACCCTAGGTCGCAAATATGATGAACCTCTCGAGATCCGTCGCGTTGATCTCACTATTCCTTCTTCCACTGCTTACCTTCTCTTTCTCCGTCGACAATCCGACGGATCGGCGAGTTCTGGTTCTTCTCGACGACCTATCACTTAAATCCTCTCATTCCATCTTCTTCAGCACTCTCAAATCGCGTGGATTCGATCTCGATTTCAAGCTAGCTGAAGATTCGAAGCTCGCGCTTCAACGGTACGGCCAGTACTTGTACGACGGGCTCATCATCTTCGCCCCCTCCACAGAGCGGTTTGGAGGATCGTTGGATTCGAAATCGATAGCTGAGTTCGTTGATTCGGGTCGTGATTTGATCTTATCGGCGGATACTTCTGCGTCTGATCTGATTCGCGGGATTGCCTTGGAGTGTGGGGTTGATTTCGATGAGGATTCTTCGGCTATGGTTATTGATCATTCCAGCTTCGACGGAGACCACACCTTGATTGCTGCAGATGATTTAGTTAAATCCCATGCCATTCTAGGAAAAACCAAGATTGAGGCTCCTGTGCTCTTCAGAGGTGTTGCACATTCATTGAACCCTACCAACAATCTGGTTTTGAAAGTTCTTTCAGCTTCTCCCTCGGCTTATTCTGCTAACCCGAGCTCGAAGTTGTCGAGTCCCCCTCAGCTTACTGGGTCTGCTATTTCGCTAGTGTCGGTCATGCAGGCCAGGAACAATGCTAGGGTTGTGATCTCAGGCTCGTTGCAGTTGTTTAGCGACAGGTTATTAAGATCTAGTGTCCAGAAAGCTGGGAGTCCAAACAAATATGAAAAATCTGGCAACGAACAGTTCGTGACTGAACTAAGCAAATGGGTCTTCCACGAAAGAGGTCATCTTAAGGCTGGGAGTCTTGTACACCACAGGGTTGGAGAAACAGATGAGCCAGCAATATATAGGATAAAGGATGACCTGGAATTTTCTGTGGAGATACTCGAGTGGTCAGGGAAGAGCTGGGAACCGTATGTCGCTGATGATATGCAGGTTCAGTTTTACATGATGAGCCCATATGTTCTGAAAACCTTGTCAACAGACAAGAAGGGTCTATTTCATACATCTTTCAAGGTTCCTGATGTGTATGGTGTATTCCAGTTTAAGGTTGAATATGAAAAGCTAGGCTACACTACACTATCTCTTTCAAAGCAGATACCTGTGAGGCCTTACAGACACAATGAGTATGAGAGATTTATCCCAACTGCGTATCCTTATTATGGAGCCTGCTTTACCACTATGGCTGGTTTCTTTGTCTTCAGCTTCGTCTACCTCTACCATAAGTAGAACATCCCGTGAATGGTTCTCCAGTCGGATAGAGTAAAATAAAGCCGAGACTTTCATTTTTAGACGAGAGAGAAAAACAAACATTTGAATCTTGTTTTTTTTTTTGCCAACAAGCATTAGTGAACACTTGCAACCTTTTTTGTTAACTAGATATTTTTAAGTGAACGTAACTTTACACTCCA
CDS Sequence
- >BnaA07g12390D
ATGATGAACCTCTCGAGATCCGTCGCGTTGATCTCACTATTCCTTCTTCCACTGCTTACCTTCTCTTTCTCCGTCGACAATCCGACGGATCGGCGAGTTCTGGTTCTTCTCGACGACCTATCACTTAAATCCTCTCATTCCATCTTCTTCAGCACTCTCAAATCGCGTGGATTCGATCTCGATTTCAAGCTAGCTGAAGATTCGAAGCTCGCGCTTCAACGGTACGGCCAGTACTTGTACGACGGGCTCATCATCTTCGCCCCCTCCACAGAGCGGTTTGGAGGATCGTTGGATTCGAAATCGATAGCTGAGTTCGTTGATTCGGGTCGTGATTTGATCTTATCGGCGGATACTTCTGCGTCTGATCTGATTCGCGGGATTGCCTTGGAGTGTGGGGTTGATTTCGATGAGGATTCTTCGGCTATGGTTATTGATCATTCCAGCTTCGACGGAGACCACACCTTGATTGCTGCAGATGATTTAGTTAAATCCCATGCCATTCTAGGAAAAACCAAGATTGAGGCTCCTGTGCTCTTCAGAGGTGTTGCACATTCATTGAACCCTACCAACAATCTGGTTTTGAAAGTTCTTTCAGCTTCTCCCTCGGCTTATTCTGCTAACCCGAGCTCGAAGTTGTCGAGTCCCCCTCAGCTTACTGGGTCTGCTATTTCGCTAGTGTCGGTCATGCAGGCCAGGAACAATGCTAGGGTTGTGATCTCAGGCTCGTTGCAGTTGTTTAGCGACAGGTTATTAAGATCTAGTGTCCAGAAAGCTGGGAGTCCAAACAAATATGAAAAATCTGGCAACGAACAGTTCGTGACTGAACTAAGCAAATGGGTCTTCCACGAAAGAGGTCATCTTAAGGCTGGGAGTCTTGTACACCACAGGGTTGGAGAAACAGATGAGCCAGCAATATATAGGATAAAGGATGACCTGGAATTTTCTGTGGAGATACTCGAGTGGTCAGGGAAGAGCTGGGAACCGTATGTCGCTGATGATATGCAGGTTCAGTTTTACATGATGAGCCCATATGTTCTGAAAACCTTGTCAACAGACAAGAAGGGTCTATTTCATACATCTTTCAAGGTTCCTGATGTGTATGGTGTATTCCAGTTTAAGGTTGAATATGAAAAGCTAGGCTACACTACACTATCTCTTTCAAAGCAGATACCTGTGAGGCCTTACAGACACAATGAGTATGAGAGATTTATCCCAACTGCGTATCCTTATTATGGAGCCTGCTTTACCACTATGGCTGGTTTCTTTGTCTTCAGCTTCGTCTACCTCTACCATAAGTAG
Protein Sequence
- >BnaA07g12390D
MMNLSRSVALISLFLLPLLTFSFSVDNPTDRRVLVLLDDLSLKSSHSIFFSTLKSRGFDLDFKLAEDSKLALQRYGQYLYDGLIIFAPSTERFGGSLDSKSIAEFVDSGRDLILSADTSASDLIRGIALECGVDFDEDSSAMVIDHSSFDGDHTLIAADDLVKSHAILGKTKIEAPVLFRGVAHSLNPTNNLVLKVLSASPSAYSANPSSKLSSPPQLTGSAISLVSVMQARNNARVVISGSLQLFSDRLLRSSVQKAGSPNKYEKSGNEQFVTELSKWVFHERGHLKAGSLVHHRVGETDEPAIYRIKDDLEFSVEILEWSGKSWEPYVADDMQVQFYMMSPYVLKTLSTDKKGLFHTSFKVPDVYGVFQFKVEYEKLGYTTLSLSKQIPVRPYRHNEYERFIPTAYPYYGACFTTMAGFFVFSFVYLYHK