Gene Details:

• Gene ID: AT5G23570
• Gene Symbol: ATSGS3, SGS3
• Gene Name: SUPPRESSOR OF GENE SILENCING 3, SUPPRESSOR OF GENE SILENCING 3
• Description: XS domain-containing protein / XS zinc finger domain-containing protein-like protein;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT5G23570.1     AT5G23570.4     AT5G23570.3     AT5G23570.2

Plant Ontology Annotations:

• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0000003  — whole plant — planta entera (Spanish, exact), 植物体全体 (Japanese, exact), clonal colony (related), colony (related), bush (narrow), frutex (narrow), frutices (narrow), gametophyte (narrow), herb (narrow), liana (narrow), prothalli (narrow), prothallium (narrow), prothallus (narrow), seedling (narrow), shrub (narrow), sporophyte (narrow), suffrutex (narrow), suffrutices (narrow), tree (narrow), vine (narrow), woody clump (narrow), genet (broad), ramet (broad)
• PO:0000013  — cauline leaf — hoja caulinar (Spanish, exact), 茎生葉、茎葉 (Japanese, exact)
• PO:0000014  — rosette leaf — hoja en roseta (Spanish, exact), ロゼット葉 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009047  — stem — caña (Spanish, exact), culm (exact), eje primario (Spanish, exact), primary axis (exact), primary stem (exact), tallo (Spanish, exact), tronco (Spanish, exact), 茎 (Japanese, exact), bole (narrow), cane (narrow), caudex (narrow), caudices (narrow), core (narrow), primocane (narrow), scape (narrow), stalk (narrow), trunk (narrow)

Germplasm Phenotype:

• CS24289  — A proportion of pre-meiotic ovules have two MMCs and have several abnormally enlarged sub-epidermal cells.
• CS24289  — Mutant plants express adult vegetative traits precociously; their vegetative phenotype is most apparent around 10-14 days after germination, when the first two leaves appear more strongly down-rolled than normal; mutant plants display variable seed set under some conditions, and also produce stigmatic tissue at the tip of the septum; defective in PTGS of sense transgenes.
• CS24290  — Mutant plants express adult vegetative traits precociously; their vegetative phenotype is most apparent around 10-14 days after germination, when the first two leaves appear more strongly down-rolled than normal; mutant plants display variable seed set under some conditions, and also produce stigmatic tissue at the tip of the septum; defective in PTGS of sense transgenes.
• CS67868  — Mutant plants express adult vegetative traits precociously; their vegetative phenotype is most apparent around 10-14 days after germination, when the first two leaves appear more strongly down-rolled than normal; mutant plants display variable seed set under some conditions, and also produce stigmatic tissue at the tip of the septum.
• CS67870  — Mutant plants express adult vegetative traits precociously; their vegetative phenotype is most apparent around 10-14 days after germination, when the first two leaves appear more strongly down-rolled than normal; mutant plants display variable seed set under some conditions, and also produce stigmatic tissue at the tip of the septum
• CS67871  — Mutant plants express adult vegetative traits precociously; their vegetative phenotype is most apparent around 10-14 days after germination, when the first two leaves appear more strongly down-rolled than normal; mutant plants display variable seed set under some conditions, and also produce stigmatic tissue at the tip of the septum.
• CS72471  — small rosettes
• CS72730  — sensor protein localizes to cytosol
• CS72731  — sensor protein localizes to cytosol
• CS72732  — CFP protein localizes to cytosol
• CS72733  — cpVenus protein localizes to cytosol
• sgs3-1  — Downward-curled leaf margins.
• sgs3-1  — Enhanced susceptibility to virus, reduction in post-transcriptional gene silencing
• sgs3-1  — Infection with cucumber mosaic virus (CMV) revealed an overaccumulation of CMV RNA compared to wild type.
• sgs3-1  — TAS1, TAS2, and TAS3 ta-siRNAs were below detectable levels.
• sgs3-1 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-2 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-3  — Infection with cucumber mosaic virus (CMV) revealed an overaccumulation of CMV RNA compared to wild type.
• sgs3-3 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-4 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-5 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-6 (L1)  — Restoration of GUS activity of the transgene through inhibition of silencing.
• sgs3-7 (2a3)  — Reduction in post-transcriptional gene silencing.
• sgs3-8 (2a3)  — Reduction in post-transcriptional gene silencing.
• sgs3-9  — Infection with cucumber mosaic virus (CMV) revealed an overaccumulation of CMV RNA compared to wild type.
• sgs3-9  — No obvious leaf developmental defects.
• sgs3-9  — TAS1, TAS2, and TAS3 ta-siRNAs were detected.
• sgs3-9 (2a3)  — Reduction in post-transcriptional gene silencing.

Function-related keywords:

• root ,  whole plant ,  cauline leaf ,  rosette leaf ,  flower ,  stem

Literature:

• Fertile hypomorphic ARGONAUTE (ago1) mutants impaired in post-transcriptional gene silencing and virus resistance.   DOI:  10.1105/tpc.010358 ;  PMID:  11910010
• The SGS3 protein involved in PTGS finds a family.   DOI:  10.1186/1471-2105-3-21 ;  PMID:  12162795
• Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance.   DOI:  10.1016/s0960-9822(03)00293-8 ;  PMID:  12747833
• Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants.   DOI:  10.1111/j.1365-313X.2004.02103.x ;  PMID:  15165191
• Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs.   DOI:  10.1016/j.molcel.2004.09.028 ;  PMID:  15469823
• SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis.   DOI:  10.1101/gad.1231804 ;  PMID:  15466488
• A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis.   DOI:  10.1101/gad.1352605 ;  PMID:  16131612
• Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis.   DOI:  10.1093/pcp/pcj057 ;  PMID:  16699177
• Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway.   DOI:  10.1016/j.cub.2006.03.064 ;  PMID:  16682355
• DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7.   DOI:  10.1016/j.cub.2006.03.035 ;  PMID:  16682354
• Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3.   DOI:  10.1242/dev.02521 ;  PMID:  16914499
• The more and smaller cells mutants of Arabidopsis thaliana identify novel roles for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in the control of heteroblasty.   DOI:  10.1242/dev.028613 ;  PMID:  19211679
• A pathogen-inducible endogenous siRNA in plant immunity.   DOI:  10.1073/pnas.0608258103 ;  PMID:  17071740
• The REDUCED LEAFLET genes encode key components of the trans-acting small interfering RNA pathway and regulate compound leaf and flower development in Lotus japonicus.   DOI:  10.1104/pp.109.140947 ;  PMID:  19955265
• Cytoplasmic Arabidopsis AGO7 accumulates in membrane-associated siRNA bodies and is required for ta-siRNA biogenesis.   DOI:  10.1038/emboj.2012.20 ;  PMID:  22327216
• RDR1 and SGS3, components of RNA-mediated gene silencing, are required for the regulation of cuticular wax biosynthesis in developing inflorescence stems of Arabidopsis.   DOI:  10.1104/pp.112.199646 ;  PMID:  22689894
• MicroRNA miR396 and RDR6 synergistically regulate leaf development.   DOI:  10.1016/j.mod.2012.07.005 ;  PMID:  22889666
• Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation.   DOI:  10.1104/pp.112.200279 ;  PMID:  22846193
• A survey of dominant mutations in Arabidopsis thaliana.   DOI:  10.1016/j.tplants.2012.08.006 ;  PMID:  22995285
• Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome.   DOI:  10.1016/j.cell.2012.10.054 ;  PMID:  23313553
• 3' fragment of miR173-programmed RISC-cleaved RNA is protected from degradation in a complex with RISC and SGS3.   DOI:  10.1073/pnas.1217050110 ;  PMID:  23417299
• Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.   DOI:  10.1186/1471-2229-13-229 ;  PMID:  24377444
• DICER-LIKE1 processed trans-acting siRNAs mediate DNA methylation: case study of complex small RNA biogenesis and action pathways in plants.   DOI:  10.4161/psb.22476 ;  PMID:  23104109
• Endogenous Arabidopsis messenger RNAs transported to distant tissues.   DOI:  10.1038/nplants.2015.25 ;  PMID:  27247031
• A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis.   DOI:  10.1104/pp.16.00148 ;  PMID:  26966170
• An H3K27me3 demethylase-HSFA2 regulatory loop orchestrates transgenerational thermomemory in Arabidopsis.   DOI:  10.1038/s41422-019-0145-8 ;  PMID:  30778176
• The RNA Export Factor ALY1 Enables Genome-Wide RNA-Directed DNA Methylation.   DOI:  10.1105/tpc.18.00624 ;  PMID:  30814259
• Capturing the phosphorylation and protein interaction landscape of the plant TOR kinase.   DOI:  10.1038/s41477-019-0378-z ;  PMID:  30833711
• Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of Arabidopsis carbon metabolism.   DOI:  10.1038/s41467-020-16289-3 ;  PMID:  32415123
• Global Analysis of RNA-Dependent RNA Polymerase-Dependent Small RNAs Reveals New Substrates and Functions for These Proteins and SGS3 in Arabidopsis.   DOI:  10.3390/ncrna7020028 ;  PMID:  33925339
• A small peptide inhibits siRNA amplification in plants by mediating autophagic degradation of SGS3/RDR6 bodies.   DOI:  10.15252/embj.2021108050 ;  PMID:  34155657
• Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in Arabidopsis.   DOI:  10.1073/pnas.2102885118 ;  PMID:  34408020
• Arabidopsis CALMODULIN-LIKE 38 Regulates Hypoxia-Induced Autophagy of SUPPRESSOR OF GENE SILENCING 3 Bodies.   DOI:  10.3389/fpls.2021.722940 ;  PMID:  34567037
• Turnip crinkle virus-encoded suppressor of RNA silencing interacts with Arabidopsis SGS3 to enhance virus infection.   DOI:  10.1111/mpp.13282 ;  PMID:  36433724

Sequences: