Gene Details:

• Gene ID: AT5G23230
• Gene Symbol: NIC2
• Gene Name: nicotinamidase 2
• Description: nicotinamidase 2;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT5G23230.1

Plant Ontology Annotations:

• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009089  — endosperm — albumen (exact), endosperma (Spanish, exact), 内胚乳、胚乳 (Japanese, exact)

Germplasm Phenotype:

• nic2-1  — A twofold decrease in nicotinamidase activity was seen in dry seed extracts of the mutant.
• nic2-1  — Freshly harvested nic2-1 seeds had a decreased level of poly(ADP)ribosylation compared to Ws seeds.
• nic2-1  — Mutant plants appeared to grow and develop normally. Their seeds phenotype have paler seed coat colour compared to wild-type Ws but the embryo was the same colour as WT.
• nic2-1  — Mutant seeds show hypersensitivity to nicotinamide (inhibitor of seed germination).
• nic2-1  — Significant 1.75-fold increase of NAD levels.
• nic2-1  — Treatment with 1 mM NADH promoted a small but significant increase in germination of mutant seeds.
• nic2-1  — Under moist-chilled (stratified) conditions, mutant seeds showed a slightly retarded germination, but there was no difference in the final percentage germination. However, in the absence of a moist chilling pre-treatment (non-stratified), germination was delayed and the germination potential of mutatn seeds was significantly lower than that of Ws, indicating a hyperdormant phenotype.

Function-related keywords:

• root ,  root ,  shoot system ,  plant embryo ,  endosperm

Literature:

• Bicistronic and fused monocistronic transcripts are derived from adjacent loci in the Arabidopsis genome.   DOI:  10.1261/rna.7114505 ;  PMID:  15659355
• Nicotinamidase participates in the salvage pathway of NAD biosynthesis in Arabidopsis.   DOI:  10.1111/j.1365-313X.2006.03013.x ;  PMID:  17335512
• Nicotinamidase activity is important for germination.   DOI:  10.1111/j.1365-313X.2007.03151.x ;  PMID:  17587307
• Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm.   DOI:  10.1007/s12192-012-0398-3 ;  PMID:  23334891

Sequences: