Gene Details:

• Gene ID: AT5G22000
• Gene Symbol: RHF2A
• Gene Name: RING-H2 group F2A
• Description: RING-H2 group F2A;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT5G22000.1     AT5G22000.2     AT5G22000.3     AT5G22000.4

Plant Ontology Annotations:

• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009049  — inflorescence — inflorescencia (Spanish, exact), 花序 (Japanese, exact), Triticeae spike (narrow)
• PO:0000084  — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)

Germplasm Phenotype:

• CS24957  — The rhf2a mutant does not have any visibly aberrant phenotypes, but, an rhf1a rhf2a double mutant reveals the role of these two proteins in gametogenesis and protein degradation.
• SALK_023787C  — The rhf2a mutant does not have any visibly aberrant phenotypes, but, an rhf1a rhf2a double mutant reveals the role of these two proteins in gametogenesis and protein degradation.
• rhf1a rhf2a  — This double mutant has shorter siliques than WT plants, and lower fertility. Approximately 60% of ovules abort the megagametophytic development process at the FG1 stage. Often, one of the megaspores that is supposed to degenerate remains visible in the rhf1a rhf2a megagametophyte for a prolonged period of time. Some of the mutants arrested at the two nucleate (F2) stage, whereas about 30% developed normal female gametophytes that could be successfully fertilized to produce viable seed. The pollen of the double mutant is largely defective with about 50-70% nonviability compared to ~0.5% nonviability for wild type pollen. The pollen for rhf1a rhf2a mutants appears to develop normally through PMI, but 30-40% of the pollen grain fail to undergo this mitotic division, and then, of those that did pass through, another 20-30% never go through the next round of cell division (PMII). The variety of different ploidy levels measured in generative cells arrested at the PMII phase indicate that they could be blocked at the G1, S, or G2. Levels of myc:KRP6 increase in the double mutant, suggesting that this protein is a putative substrate of this E3 ubiquitin ligase. And many of the aberrant phenotypes observed in the double mutant can be improved when KRP6 levels are decreased by RNAi.

Function-related keywords:

• root ,  inflorescence ,  plant sperm cell

Literature:

• The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors.   DOI:  10.1093/jxb/ern155 ;  PMID:  18552355
• Targeted degradation of the cyclin-dependent kinase inhibitor ICK4/KRP6 by RING-type E3 ligases is essential for mitotic cell cycle progression during Arabidopsis gametogenesis.   DOI:  10.1105/tpc.108.059741 ;  PMID:  18552199
• Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.   DOI:  10.1104/pp.108.121038 ;  PMID:  18650403
• Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.   DOI:  10.1104/pp.108.126375 ;  PMID:  18775970
• Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.   DOI:  10.1074/mcp.M600429-MCP200 ;  PMID:  17317660
• Repertoire of plant RING E3 ubiquitin ligases revisited: New groups counting gene families and single genes.   DOI:  10.1371/journal.pone.0203442 ;  PMID:  30169501
• Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.   DOI:  10.1074/mcp.M600429-MCP200 ;  PMID:  17317660
• Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.   DOI:  10.1074/mcp.M600429-MCP200 ;  PMID:  17317660

Sequences: