Gene Details:
- Gene ID: AT5G19550
- Gene Symbol: AAT2, ASP2
- Gene Name: ASPARTATE AMINOTRANSFERASE 2, aspartate aminotransferase 2
- Description: aspartate aminotransferase 2;(source:Araport11)
- TAIR Accession: locus:2180826
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
- PO:0000084 — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)
- PO:0000293 — guard cell — célula guardiana (Spanish, exact), occlusive cell (exact), 孔辺細胞 (Japanese, exact)
Gene Ontology:
- GO:0071456 — acts upstream of or within — cellular response to hypoxia
- GO:0009536 — located in — plastid
- GO:0006807 — acts upstream of or within — nitrogen compound metabolic process
- GO:0004069 — enables — L-aspartate:2-oxoglutarate aminotransferase activity
- GO:0006103 — involved in — 2-oxoglutarate metabolic process
- GO:0006531 — involved in — aspartate metabolic process
- GO:0005886 — located in — plasma membrane
- GO:0005739 — is active in — mitochondrion
- GO:0005737 — located in — cytoplasm
- GO:0005829 — located in — cytosol
- GO:0005507 — enables — copper ion binding
- GO:0009058 — involved in — biosynthetic process
- GO:0009506 — located in — plasmodesma
- GO:0006536 — involved in — glutamate metabolic process
- GO:0030170 — enables — pyridoxal phosphate binding
- GO:0009505 — located in — plant-type cell wall
- GO:0000325 — located in — plant-type vacuole
Germplasm Phenotype:
- CS3971 — deficient in cytosolic aspartate aminotransferase (AAT2) isoenzyme activity on native gels; reduced root growth rate, short roots on MS; displays about 80% reduction in levels of aspartate transported in the phloem of light-grown plants, and about 50% reduction in levels of asparagine transported in dark-adapted plants.
- CS3972 — deficient in cytosolic aspartate aminotransferase (AAT2) isoenzyme activity on native gels; no visible phenotypic effect and no aspartate deficiency.
- CS3973 — deficient in cytosolic aspartate aminotransferase (AAT2) isoenzyme activity on native gels; no visible phenotypic effect and no aspartate deficiency
Function-related keywords:
- root , pollen , plant sperm cell , guard cell
Literature:
- Arabidopsis mutants define an in vivo role for isoenzymes of aspartate aminotransferase in plant nitrogen assimilation. DOI: 10.1093/genetics/149.2.491 ; PMID: 9611168
- Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotransferase from Arabidopsis thaliana. DOI: 10.1006/prep.1997.0845 ; PMID: 9535706
- The aspartate aminotransferase gene family of Arabidopsis encodes isoenzymes localized to three distinct subcellular compartments. DOI: 10.1046/j.1365-313x.1995.07010061.x ; PMID: 7894512
- Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. DOI: 10.1105/tpc.006130 ; PMID: 12509522
- a high-density colony array study (HDCA). DOI: 10.1046/j.1365-313x.2003.01847.x ; PMID: 12969430
- Generation of Arabidopsis protein chips for antibody and serum screening. DOI: 10.1023/a:1025424814739 ; PMID: 14558660
- Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. DOI: 10.1093/pcp/pch099 ; PMID: 15215502
- Transcript profiling in the chl1-5 mutant of Arabidopsis reveals a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1. DOI: 10.1105/tpc.104.024380 ; PMID: 15319483
- Global transcription profiling reveals comprehensive insights into hypoxic response in Arabidopsis. DOI: 10.1104/pp.104.055475 ; PMID: 15734912
- A genome-wide analysis of the effects of sucrose on gene expression in Arabidopsis seedlings under anoxia. DOI: 10.1104/pp.104.057299 ; PMID: 15734908
- Coordination of nuclear and mitochondrial genome expression during mitochondrial biogenesis in Arabidopsis. DOI: 10.1105/tpc.104.030254 ; PMID: 15829605
- Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation. DOI: 10.1093/aob/mci217 ; PMID: 16081496
- Proteome mapping of mature pollen of Arabidopsis thaliana. DOI: 10.1002/pmic.200402011 ; PMID: 16247729
- Dynamics of Arabidopsis thaliana soluble proteome in response to different nutrient culture conditions. DOI: 10.1002/elps.200500561 ; PMID: 16358359
- Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. DOI: 10.1104/pp.106.082057 ; PMID: 16679420
- Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes. DOI: 10.1186/gb-2006-7-8-R76 ; PMID: 16916443
- Proteomic analysis of seed dormancy in Arabidopsis. DOI: 10.1104/pp.106.087452 ; PMID: 17028149
- An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach. DOI: 10.1105/tpc.106.044743 ; PMID: 17172353
- Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. DOI: 10.1104/pp.108.121038 ; PMID: 18650403
- Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function. DOI: 10.1104/pp.109.147942 ; PMID: 20018591
- Systems analysis of seed filling in Arabidopsis: using general linear modeling to assess concordance of transcript and protein expression. DOI: 10.1104/pp.109.152413 ; PMID: 20118269
- root uv-b sensitive mutants are suppressed by specific mutations in ASPARTATE AMINOTRANSFERASE2 and by exogenous vitamin B6. DOI: 10.1093/mp/ssr033 ; PMID: 21511809
- Phloem-specific expression of Yang cycle genes and identification of novel Yang cycle enzymes in Plantago and Arabidopsis. DOI: 10.1105/tpc.110.079657 ; PMID: 21540433
- Endogenous Arabidopsis messenger RNAs transported to distant tissues. DOI: 10.1038/nplants.2015.25 ; PMID: 27247031
- Integrative Analysis from the Epigenome to Translatome Uncovers Patterns of Dominant Nuclear Regulation during Transient Stress. DOI: 10.1105/tpc.19.00463 ; PMID: 31519798
- Proteome mapping of mature pollen of Arabidopsis thaliana. DOI: 10.1002/pmic.200402011 ; PMID: 16247729
- Arabidopsis cell wall proteome defined using multidimensional protein identification technology. DOI: 10.1002/pmic.200500046 ; PMID: 16287169
- A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture. DOI: 10.1074/mcp.M600250-MCP200 ; PMID: 17151019
- A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome. DOI: 10.1074/mcp.M700099-MCP200 ; PMID: 17644812
- Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling. DOI: 10.1016/j.jprot.2017.06.004 ; PMID: 28627464
Sequences:
cDNA Sequence
- >AT5G19550.1
AATATTTCTCGTATAAAAAAAAAATGTAAATAAAATTTCTCGACTTGTTGGGCAAGTTCAAGCATCGCCCACAGTTGCCAAATCGCAATATAGGCGCCCACAGCTAAAAGTTGATTCTCAAAACCTCTCTCTCTTTGTGAACAAGATTAAAAAGACGACTTCTCTTTTAACTTATTCCTCCTCTGCTTCTTCAAGATCCATTGTCTGCAATTTCAATCCCTTGTCTCGTCATGGATTCCGTCTTCTCTAACGTTGCTCGTGCTCCCGAAGATCCTATTCTCGGTGTAACTGTTGCTTACAACAATGATCCTAGTCCTGTTAAGATCAATTTGGGTGTCGGTGCCTACCGAACTGAGGAAGGGAAGCCTCTTGTTCTTGATGTGGTGCGTAAGGCAGAGCAACAGCTAGTGAACGATCCGTCTCGGGTCAAGGAATACATTCCCATTGTTGGTATTTCCGATTTTAACAAGTTAAGCGCCAAGCTCATCTTAGGTGCTGACAGTCCTGCGATTACAGAAAGTAGAGTTACTACAGTCCAGTGCTTGTCTGGTACTGGTTCGTTGAGAGTTGGGGCTGAGTTTCTCAAAACACACTACCACCAAAGTGTCATTTACATTCCAAAACCAACTTGGGGGAACCATCCCAAAGTTTTCAACTTGGCAGGCTTGTCTGTGGAGTATTTCCGCTACTATGATCCTGCAACCCGTGGTCTTGACTTCAAAGGCTTGCTCGAGGATCTTGGCGCTGCACCATCTGGAGCTATTGTCTTACTTCATGCATGTGCACACAATCCCACTGGAGTTGACCCAACCTCTGAACAGTGGGAACAGATTCGACAGCTGATGAGATCTAAAAGCTTATTACCCTTTTTTGATAGTGCATATCAGGGTTTTGCTAGTGGTAGCCTTGACACAGATGCACAATCTGTCCGTACATTTGTTGCTGATGGCGGTGAATGCTTGATAGCTCAAAGTTACGCCAAAAATATGGGACTTTATGGGGAGCGTGTTGGTGCCCTTAGCATTGTCTGCAAGTCAGCAGATGTGGCTAGTAAGGTTGAGAGCCAGGTGAAGCTTGTTGTGCGGCCCATGTATTCGAGCCCCCCTATTCATGGAGCATCAATTGTTGCCACCATTCTAAAAAGCAGTGATATGTACAACAACTGGACCATCGAGCTGAAAGAAATGGCTGACCGTATAAAGAGCATGCGCCAACAGTTATTTGAAGCTATACAAGCTAGAGGTACACCTGGTGACTGGAGTCATATTATCAAACAGATTGGGATGTTTACATTTACTGGATTGAACAAGGAGCAAGTTGAATTCATGACCAAAGAGTTCCACATTTACATGACCTCTGATGGGAGAATAAGCATGGCAGGTCTAAGTTCGAAGACAGTGCCTCACCTTGCCGATGCTATGCATGCTGCAGTGACCCGCCTCGGCTAAACTAGCGACCATACATTTTTTTGCTGTTTCTACAAATAAAATGGGACAAAAAAAAAGAACACCGTTTTTGTTGGCTTATATACTATGGTTTTTTTCTGATGGTACCATATGTTTCCTCATGTCTTTTGACTAAAGATGGTTGAACCAAAACCAAAGTGAGGATTGTGACATTGAGATCTACCGTTTCTAAGAAAATATTGGTTACTTTAATTTGCCACCAACAATTGTTATTTTGCAGAATTTTCAAATGTCCTGTTATTTTATTTGCTGAGAGATCCTTATAGTTTGGTTCTCGCTGGAAAAAAATAAGTTTTGGGAAGTTTGGCACACCTAAGAAGAAGTATGATTTGTATTAGAAAAAAAAATACTTGTCG
CDS Sequence
Protein Sequence