Gene Details:

  • Gene ID: AT5G17290
  • Gene Symbol: APG5, ATATG5, ATG5
  • Gene Name: AUTOPHAGY 5, AUTOPHAGY 5
  • Description: autophagy protein Apg5 family;(source:Araport11)
  • TAIR Accession: locus:2167195
  • Genome: Araport11_genome_release
  • Species: Arabidopsis thaliana

Transcripts:

Plant Ontology Annotations:

  • PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
  • PO:0000084  — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)

Gene Ontology:

  • GO:0050832  — acts upstream of or within — defense response to fungus
  • GO:0006501  — involved in — C-terminal protein lipidation
  • GO:0006914  — acts upstream of or within — autophagy
  • GO:0005737  — located in — cytoplasm
  • GO:0044804  — involved in — nucleophagy
  • GO:0006995  — involved in — cellular response to nitrogen starvation
  • GO:0010150  — acts upstream of or within — leaf senescence
  • GO:0019776  — contributes to — Atg8-family ligase activity
  • GO:0000045  — involved in — autophagosome assembly
  • GO:0034045  — is active in — phagophore assembly site membrane
  • GO:0000422  — involved in — autophagy of mitochondrion
  • GO:0034274  — part of — Atg12-Atg5-Atg16 complex
  • GO:0042594  — acts upstream of or within — response to starvation

Germplasm Phenotype:

  • CS39993  — Under a short-day photoperiod (8h light/16h dark), the atg5-1 plants grew slower resulting in smaller plants, flowered later with reduced fecundity, and showed enhanced senescence of rosette leaves than wild type; hypersensitive to either nitrogen or carbon deprivation.
  • SAIL_129_B07  — early senescence; accumulation of high levels of reactive oxygen species.
  • atg5-1  — The T-DNA insertion prevents accumulation of the ATG5 mRNA in the mutants. Absence of 50-kD and a 40-kD proteins, which correspond to the sizes of the conjugate ATG12-ATG5 and the ATG5 proteins, respectively. Homozygous atg5-1 seedlings germinated and developed normally, and flowered and set seed at the same rate as wild-type plants under a long-day photoperiod (16h light/8h dark). Under a short-day photoperiod (8h light/16h dark), the atg5-1 plants grew slower resulting in smaller plants, flowered later than wild type with reduced fecundity, and showed enhanced senescence of rosette leaves. Hypersensitive to N-deficient growth conditions (mutants presented enhanced chlorosis of the cotyledons and reduced true leaf formation). Low recovery rates (20%) after restoration of N in the media, as compared to 100% recovery rates in wildtype. Hypersensitive to carbon starvation induced by darkness (plants become severely chlorotic and flaccid faster and protein loss was higher than in wild type). In C-starved plants the protein levels of ATG7 decreased and the ATG5 increased in the mutant, whereas the protein levels of ATG8 did not drop, but the ATG8 mRNA level increased, unlike in wild type.
  • lon2-2 atg5-5  — IBA responsiveness to lon2-2 restored
  • lon2-2 atg5-6  — IBA responsiveness to lon2-2 restored

Literature:

Sequences:

cDNA Sequence
  • >AT5G17290.1
    CGTACGCCTTATTAATACCACATAAAGACGCAAGAGAAGATGACGTCATACAGAAACAGCGTCGTTTTGAAAGCTGAGTTTTTTTTGTGAAGCGAGCGAGCGTGAGAAGGTGTGACGGGGAAGAGAATGGCGAAGGAAGCGGTCAAGTATGTATGGGAAGGAGCAATTCCTCTGCAGATTCATCTCCACAAATCCGACGTCGCTTCTCACCCTGCTCCTCCTCCTGCTCTTGTGTTAGCACCAAGAATAGGATATTTGCCTCTGTTGATTCCTCTTATAAAGCCTTATTTCAAGGATTCACTTCCTCCTGGTGAAGATTCAATTTGGTTTGATTACAAAGGATTTCCTCTAAAATGGTATATACCAACAGGTGTTCTTTTCGATCTCCTTTGTGCAGAACCCGAAAGACCATGGAATCTCACGATACACTTTAGAGGATATCCTTGCAACATACTGATACCATGTGAAGGAGAAGATTCTGTAAAATGGAACTTTGTTAATTCTTTGAAAGAGGCACAATATATCATCAATGGAAATTGCAAGAATGTTATGAACATGTCTCAGAGTGATCAAGAGGATCTATGGACCTCTGTCATGAACGGTGATCTTGATGCCTATACAAGATTATCACCCAAGCTTAAAATGGGAACAGTCGAAGATGAGTTTTCAAGGAAAACAAGTTTGTCATCTCCACAATCTCAACAAGTTGTGCCTGAGACGGAGGTGGCTGGACAAGTTAAGACAGCAAGAATTCCTGTTCGGTTGTATGTTCGAAGTCTAAATAAAGATTTCGAGAATCTTGAAGATGTACCGGAGATCGATACCTGGGATGACATCTCGTACCTTAATCGCCCTGTTGAGTTCCTCAAAGAAGAAGGGAAATGCTTTACGTTACGTGACGCCATTAAAAGTCTCCTCCCTGAGTTTATGGGAGACAGAGCGCAAACGAGTGGTGAAGAAAGAAGCATAGATGATACAGAAGAAGCAGATGGGTCGAGGGAGATGGGTGAAATCAAATTGGTAAGGATACAAGGGATAGAAATGAAGCTAGAGATACCGTTTTCGTGGGTGGTAAATAACTTGATGAACCCAGAATTCTATCTCCATATCTCTGTCCTTGTGAAAGCTCCTCAAAGGTGAAGTGTAAGGTTCTCTGCAGTTACAATCCATCTGTGAATTGAATCAAATTGCTTTCTCGTTCCATCTTACAAATCCGAAAGAATCAATGATTTGTTGTATACAGCTACTTCTTCTATTTCTGATAGAAGCAAGAACACAGAATACAGAAAAGAAAGTAGAAATATTTTGATTGACTCGTTAGCTCTTTTACATTTGTTACTTGACGGTTTCCTCTCTGAGCAAATTTGATTGAAATCAATTTATGGTCGATATAATAATCACTACTTTCTTCTATTCACAAGTAAATCAGCTCAAGTTCATAGAGAGATCCAAAATGTTGTTCAACAAAAGCACTGTATCATCAAATCGACAAAGACAAATGTTTAATTTCTCCATTAACAATGCGAGGAAAAATCAGTCAAATTGATTTAGTTTCTCCAGAAAGCTGCTTGTTGTAATCTTCGAGCTCATCGTAATAAACATCCCAAACGCATCGGACGCAACCGCTACCGCAACAATCGCCAGGCTCTGGTTTCTCTGGCGGTGGAGGAACCGAAACTCCCACCTCCTTCTTATCTTCTGTCTCCTTCTTGTTTGTCTCCTCCTTGGAAGCTTCTACAAGATTCTTGCTCTCGGTCGTCGCCATGGTCACCGGAGATTTGAGCTGTTGTTCTGGAGATACGGAAGTGATTGAATAATTGGAGAAAGATCGTTTGAGAGGGAGATGTCGCGTCAAACCAAATCTCATGCTCAAAAGCACATCGTGAAGGCTAGAACCCGGTGATGTAACGATCGAGATTCGAGGAAGAAGAGACACAACAACCATACGCACAGATCAAAAAGATTTGTTCTCTTCTTTTTTTTTCTATTTCCTTTTTTCGTGTCAGAATTGATTTCTTTATGACCTGTCATCCTATAGTTGGCAAAAAAAGCCGCCAATCATAAACGGCACGCATATTTATCTTCTAAACGTTAAAACGGCACGACATCTTTTTTGAACACAAGAACCAACAACTTCTTGATCTTCAGCTGCGCCATGATTTTGA
CDS Sequence
Protein Sequence