Gene Details:
- Gene ID: AT5G14620
- Gene Symbol: DMT7, DRM2
- Gene Name: domains rearranged methyltransferase 2
- Description: domains rearranged methyltransferase 2;(source:Araport11)
- TAIR Accession: locus:2222627
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0009025 — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
- PO:0009049 — inflorescence — inflorescencia (Spanish, exact), 花序 (Japanese, exact), Triticeae spike (narrow)
- PO:0000084 — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)
Gene Ontology:
- GO:0006306 — acts upstream of or within — DNA methylation
- GO:0050832 — acts upstream of or within — defense response to fungus
- GO:0005654 — located in — nucleoplasm
- GO:0006306 — involved in — DNA methylation
- GO:0009008 — enables — DNA-methyltransferase activity
- GO:0006346 — involved in — DNA methylation-dependent heterochromatin formation
- GO:0005515 — enables — protein binding
- GO:0005634 — located in — nucleus
- GO:0005634 — is active in — nucleus
- GO:0003886 — enables — DNA (cytosine-5-)-methyltransferase activity
- GO:0008170 — enables — N-methyltransferase activity
Germplasm Phenotype:
- CS16383 — No visible phenotype.
- CS16384 — Plants show developmental phenotypes, including reduced plant size and curled leaves.
- CS16386 — No visible phenotype.
- CS16387 — Unusual development patterns and plant structure; phenotype develops after inbreeding.
- CS16388 — Reduced plant size; curled leaves.
- CS6366
— Wild type morphology; double mutant that retains preexixting CpG methylation at the endogenous FWA locus but lacks de novo CpG methylation normaly associated with FWA transgene silencing; blocks de novo CpNpG and asymmetric methylation and gene silencing of the endogenous Superman (SUP) gene, which is normally triggered by an inverted SUP repeat, doesnt show reactivation of previously established SUP epigenetic silenced alleles 12121623
AT5G14620 CS69842 Reactivation of GFP fluorescence in root meristems of seedlings. AT5G14620 CS69843 Reactivation of GFP fluorescence in root meristems of seedlings. AT5G14620 CS69844 Reactivation of GFP fluorescence in root meristems of seedlings. AT5G14620 CS72761 Quintuple homozygous plants exhibit extreme growth retardation and small size, display a suite of severe developmental defects, and do not undergo floral transition. AT5G14620 CS9605 impaired gene silencing, reduced de novo methylation AT5G14660 def2 Reduced seed weight AT5G14720 SALK_065417 significantly shorter hypocotyl at 28 °C 33990595
AT5G14720 SALK_086087 significantly shorter hypocotyl at 28 °C 33990595
AT5G14750 CS6349 Altered epidermal cell determination in the root and hypocotyl (but not leaf); extra root hair cells are produced in the root (hairy root); abnormally distributed stomata are produced in the hypocotyl. AT5G14750 CS67773 Excessive root hair production. GFP is expressed in all stages of differentiating root epidermal cells, in both hair (H) and non-hair (N) cell types, although preferentially in the N cells. AT5G14800 SALK_067384 Reduced activity of PFK in 7-day-old seedlings. Reduced root length. 33800095
AT5G14870 cngc18-1 Pollen tubes show severe growth defect. Tubes are kinky, short, and often thin. Tubes grow only a short distance with nondirectional growth, often terminating prematurely with a bursting event. 17726111
AT5G14950 SALK_052443 Defective in the formation of hybrid and complex N-glycans. Viable and fertile and do not show any obvious phenotype under standard growth conditions. 11432912
AT5G15070 CS2109994 seedling lethal AT5G15070 CS2110007 seedling lethal AT5G15070 CS2110008 smaller rosette AT5G15120 SALK_108381C “Seedlings screened in bulk via high throughput methods for responses to various stressors. Results are in comparison to Col-0.heat:decreased,cold:decreased,oxidative stress:decreased,osmotic stress:no change,salt stress:no change,ABA:no change,hypoxia:ND.” 23517122
AT5G15160 bnq1 rnai; bnq2 rnai; bnq3 Same as bnq3 single mutants: sepals and carpels are pale yellow or white,while the inflorescence stems and siliques are purple 20305124
AT5G15160 bnq2 rnai WT phenotype 20305124
AT5G15170 SALK_119060C No obvious morphological defect. No increased sensitivity to CPT, a DNA protein crosslink inducing compound. 30760561
AT5G15190 SALK_079419C “Seedlings screened in bulk via high throughput methods for responses to various stressors. Results are in comparison to Col-0.heat:no change,cold:no change,oxidative stress:no change,osmotic stress:no change,salt stress:decreased,ABA:no change,hypoxia:ND.” 23517122
AT5G15250 SALK_012429 No visible change in plant growth, development or pigmentation. 16157880
AT5G15250 ftsh6 no visible difference in leaf morphology and variegation when grown under normal light and temperature conditions compared to wild-type. 14630971
AT5G15380 CS6366 Wild type morphology; double mutant that retains preexixting CpG methylation at the endogenous FWA locus but lacks de novo CpG methylation normaly associated with FWA transgene silencing; blocks de novo CpNpG and asymmetric methylation and gene silencing of the endogenous Superman (SUP) gene, which is normally triggered by an inverted SUP repeat, doesnt show reactivation of previously established SUP epigenetic silenced alleles
Function-related keywords:
Literature:
- Conserved plant genes with similarity to mammalian de novo DNA methyltransferases. DOI: 10.1073/pnas.97.9.4979 ; PMID: 10781108
- Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing. DOI: 10.1016/s0960-9822(02)00925-9 ; PMID: 12121623
- Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes. DOI: 10.1073/pnas.162371599 ; PMID: 12151602
- An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana. DOI: 10.1016/j.cell.2006.05.032 ; PMID: 16839879
- The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center. DOI: 10.1016/j.cell.2006.05.031 ; PMID: 16839878
- Regulation of seed size by hypomethylation of maternal and paternal genomes. DOI: 10.1104/pp.106.088849 ; PMID: 17012404
- Two-step recruitment of RNA-directed DNA methylation to tandem repeats. DOI: 10.1371/journal.pbio.0040363 ; PMID: 17105345
- The SRA methyl-cytosine-binding domain links DNA and histone methylation. DOI: 10.1016/j.cub.2007.01.009 ; PMID: 17239600
- Cell biology of the Arabidopsis nuclear siRNA pathway for RNA-directed chromatin modification. DOI: 10.1101/sqb.2006.71.046 ; PMID: 17381329
- Genetic interactions between DNA demethylation and methylation in Arabidopsis. DOI: 10.1104/pp.107.107730 ; PMID: 17951456
- ARGONAUTE4 is required for resistance to Pseudomonas syringae in Arabidopsis. DOI: 10.1105/tpc.107.054494 ; PMID: 17993621
- Tandem repeats upstream of the Arabidopsis endogene SDC recruit non-CG DNA methylation and initiate siRNA spreading. DOI: 10.1101/gad.1667808 ; PMID: 18559476
- Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells. DOI: 10.1371/journal.pone.0003306 ; PMID: 18827894
- RNAi, DRD1, and histone methylation actively target developmentally important non-CG DNA methylation in arabidopsis. DOI: 10.1371/journal.pgen.0020083 ; PMID: 16741558
- Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. DOI: 10.1371/journal.pgen.0040027 ; PMID: 18266474
- Multimegabase silencing in nucleolar dominance involves siRNA-directed DNA methylation and specific methylcytosine-binding proteins. DOI: 10.1016/j.molcel.2008.11.009 ; PMID: 19061642
- SRA-domain proteins required for DRM2-mediated de novo DNA methylation. DOI: 10.1371/journal.pgen.1000280 ; PMID: 19043555
- Defining the functional network of epigenetic regulators in Arabidopsis thaliana. DOI: 10.1093/mp/ssp017 ; PMID: 19825647
- and AGO4-associated protein acts in RNA-directed DNA methylation. DOI: 10.1038/nature09025 ; PMID: 20410883
- directed DNA methylation in Arabidopsis. DOI: 10.1016/j.cub.2010.03.062 ; PMID: 20409711
- A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA. DOI: 10.1371/journal.pgen.1000690 ; PMID: 19834541
- Involvement of a Jumonji-C domain-containing histone demethylase in DRM2-mediated maintenance of DNA methylation. DOI: 10.1038/embor.2010.158 ; PMID: 21052090
- Identification of genes required for de novo DNA methylation in Arabidopsis. DOI: 10.4161/epi.6.3.14242 ; PMID: 21150311
- SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. DOI: 10.1371/journal.pgen.1002195 ; PMID: 21811420
- Small RNAs prevent transcription-coupled loss of histone H3 lysine 9 methylation in Arabidopsis thaliana. DOI: 10.1371/journal.pgen.1002350 ; PMID: 22046144
- The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis. DOI: 10.4161/epi.7.1.18782 ; PMID: 22274613
- The RNA silencing enzyme RNA polymerase v is required for plant immunity. DOI: 10.1371/journal.pgen.1002434 ; PMID: 22242006
- Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome. DOI: 10.1016/j.cell.2012.10.054 ; PMID: 23313553
- Generation of a luciferase-based reporter for CHH and CG DNA methylation in Arabidopsis thaliana. DOI: 10.1186/1758-907X-4-1 ; PMID: 23561294
- An 11bp region with stem formation potential is essential for de novo DNA methylation of the RPS element. DOI: 10.1371/journal.pone.0063652 ; PMID: 23671690
- RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA. DOI: 10.1111/tpj.12563 ; PMID: 24862207
- Molecular mechanism of action of plant DRM de novo DNA methyltransferases. DOI: 10.1016/j.cell.2014.03.056 ; PMID: 24855943
- MiRNAting control of DNA methylation. DOI: 10.1007/s12038-014-9437-9 ; PMID: 24845501
- High-temperature effect on genes engaged in DNA methylation and affected by DNA methylation in Arabidopsis. DOI: 10.1016/j.plaphy.2014.12.022 ; PMID: 25576840
- A JUMONJI Protein with E3 Ligase and Histone H3 Binding Activities Affects Transposon Silencing in Arabidopsis. DOI: 10.1104/pp.15.01688 ; PMID: 26979329
- Two Components of the RNA-Directed DNA Methylation Pathway Associate with MORC6 and Silence Loci Targeted by MORC6 in Arabidopsis. DOI: 10.1371/journal.pgen.1006026 ; PMID: 27171427
- Plant DNA Methyltransferase Genes: Multiplicity, Expression, Methylation Patterns. DOI: 10.1134/S0006297916020085 ; PMID: 27260394
- Characterization of microRNAs and their target genes associated with transcriptomic changes in gamma-irradiated Arabidopsis. DOI: 10.4238/gmr.15038386 ; PMID: 27525891
- AGO4 is specifically required for heterochromatic siRNA accumulation at Pol V-dependent loci in Arabidopsis thaliana. DOI: 10.1111/tpj.13463 ; PMID: 28002617
- Research progress on the autonomous flowering time pathway in Arabidopsis. DOI: 10.1007/s12298-017-0458-3 ; PMID: 28878488
- Analysis of the DNA methylation patterns and transcriptional regulation of the NB-LRR-encoding gene family in Arabidopsis thaliana. DOI: 10.1007/s11103-018-0715-z ; PMID: 29525832
- APURINIC/APYRIMIDINIC ENDONUCLEASE2 and ZINC FINGER DNA 3'-PHOSPHOESTERASE Play Overlapping Roles in the Maintenance of Epigenome and Genome Stability. DOI: 10.1105/tpc.18.00287 ; PMID: 30135084
- Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana. DOI: 10.1093/pcp/pcy197 ; PMID: 30295898
- Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment. DOI: 10.1016/j.plantsci.2018.12.029 ; PMID: 30824017
- Rare earth elements regulate the endocytosis and DNA methylation in root cells of Arabidopsis thaliana. DOI: 10.1016/j.chemosphere.2019.04.076 ; PMID: 31004819
- DNA methylation repels targeting of Arabidopsis REF6. DOI: 10.1038/s41467-019-10026-1 ; PMID: 31048693
- Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis. DOI: 10.1093/nar/gkz636 ; PMID: 31372641
- The Histone Demethylase IBM1 Positively Regulates Arabidopsis Immunity by Control of Defense Gene Expression. DOI: 10.3389/fpls.2019.01587 ; PMID: 31956325
- Transgenerational effect of mutants in the RNA-directed DNA methylation pathway on the triploid block in Arabidopsis. DOI: 10.1186/s13059-021-02359-2 ; PMID: 33957942
- In Arabidopsis thaliana Cd differentially impacts on hormone genetic pathways in the methylation defective ddc mutant compared to wild type. DOI: 10.1038/s41598-021-90528-5 ; PMID: 34040101
- The DNA methylation landscape of the root-knot nematode-induced pseudo-organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism. DOI: 10.1111/nph.18395 ; PMID: 35872574
- DEMETHYLATION REGULATOR 1 regulates DNA demethylation of the nuclear and mitochondrial genomes. DOI: 10.1111/jipb.13386 ; PMID: 36223079
- Establishment and maintenance of DNA methylation in nematode feeding sites. DOI: 10.3389/fpls.2022.1111623 ; PMID: 36704169
- RETINOBLASTOMA-RELATED interactions with key factors of the RNA-directed DNA methylation (RdDM) pathway and its influence on root development. DOI: 10.1007/s00425-023-04135-x ; PMID: 37120771
- DDT-RELATED PROTEIN4-IMITATION SWITCH alters nucleosome distribution to relieve transcriptional silencing in Arabidopsis. DOI: 10.1093/plcell/koad143 ; PMID: 37208763
Sequences:
cDNA Sequence
- >AT5G14620.1
GTTTCCTCCTCCAGTAAACTGACGACGATACAAGCTCTCTCACCAACGGCGACAGTATCTTTGCCGGCGGCGGGAGCGCTTCTGCTTAATCTGTAGCTCAAACTAAAAATTCTCTGGGTATTACTCATGGTGATTTGGAATAACGATGATGATGATTTTTTGGAGATTGATAACTTTCAATCTTCTCCACGGTCATCTCCAATACATGCAATGCAGTGTAGGGTCGAAAATCTAGCTGGTGTAGCCGTGACAACTAGTTCTTTGAGCTCTCCTACTGAGACAACTGATTTAGTTCAGATGGGCTTCTCAGACGAGGTTTTTGCTACATTGTTTGACATGGGATTTCCTGTTGAGATGATTTCTAGAGCGATCAAGGAAACTGGACCAAATGTAGAAACTTCGGTTATAATTGATACTATCTCCAAATACTCAAGCGACTGTGAAGCTGGTTCTTCCAAGTCCAAGGCTATTGATCATTTCCTTGCTATGGGATTTGATGAAGAAAAAGTTGTCAAAGCCATTCAAGAACATGGAGAAGACAATATGGAAGCAATTGCAAATGCATTGCTCTCTTGTCCAGAGGCTAAGAAACTGCCAGCAGCAGTAGAGGAAGAAGATGGCATTGACTGGTCATCAAGTGATGATGATACCAATTACACCGATATGTTAAACTCAGATGATGAGAAAGATCCAAACTCAAATGAAAATGGCAGCAAAATACGGTCTTTGGTGAAGATGGGTTTCTCAGAGCTTGAAGCTTCTTTAGCTGTCGAGAGATGTGGAGAAAATGTGGATATTGCAGAGCTCACAGACTTCCTTTGTGCTGCTCAAATGGCTAGGGAATTTAGTGAGTTTTACACTGAACATGAAGAACAAAAGCCTAGACATAATATTAAGAAAAGGCGGTTTGAGTCAAAAGGAGAGCCAAGATCATCTGTTGATGACGAGCCGATTCGTCTACCAAATCCAATGATAGGATTTGGGGTTCCAAACGAGCCCGGACTCATTACACATAGATCGCTTCCAGAGTTAGCCCGAGGGCCACCTTTTTTCTACTATGAGAATGTCGCCCTCACACCTAAAGGCGTTTGGGAGACTATTTCCAGGCACTTGTTCGAGATCCCACCTGAGTTTGTGGACTCAAAATATTTCTGTGTTGCAGCGAGGAAGAGAGGCTACATCCACAATCTCCCCATCAACAACAGATTTCAGATTCAGCCTCCACCAAAATACACCATCCATGATGCATTTCCTTTGAGTAAGAGATGGTGGCCAGAATGGGATAAAAGGACCAAGCTTAATTGCATTTTGACTTGTACAGGTAGTGCTCAGTTGACTAACAGGATTCGTGTAGCCCTTGAGCCTTACAATGAAGAACCAGAACCGCCTAAGCATGTACAAAGATATGTGATTGACCAGTGCAAAAAATGGAATTTGGTTTGGGTGGGTAAAAACAAAGCTGCCCCACTCGAGCCAGATGAGATGGAGAGTATTCTGGGATTTCCAAAAAATCATACTCGTGGTGGAGGCATGAGTAGAACTGAGCGCTTCAAGTCCTTAGGAAATTCGTTTCAGGTTGATACTGTGGCGTATCATCTGTCTGTCCTGAAGCCCATTTTCCCACATGGAATCAATGTTCTCTCTCTTTTCACGGGTATTGGTGGTGGGGAAGTGGCACTTCATCGTCTCCAAATCAAAATGAAGCTTGTTGTGTCTGTTGAGATTTCAAAAGTCAACAGAAATATTTTGAAGGACTTTTGGGAGCAAACTAACCAGACTGGAGAATTGATCGAGTTTTCAGACATCCAACACTTGACTAATGACACAATCGAAGGGTTGATGGAGAAATATGGTGGATTTGATCTTGTAATTGGAGGAAGTCCTTGTAACAATCTGGCAGGCGGTAATAGGGTAAGCCGAGTTGGTCTTGAAGGTGATCAATCTTCGTTGTTCTTTGAGTATTGCCGTATTCTAGAGGTGGTACGTGCGAGGATGAGAGGATCTTGATCATTCACATTACCTGAAGTAGATTGAAGTAGATATGACTCCATGAAATCTAGTTGAACTTTATTTTTCTATATCTGTTATGTAATATGGCCATGAAGGTACATTTTTGAATAATATAAGTCAACCTAACTCTCTTTTGCTTTTTGCGACAATATATCTGCAACTGCATTTGCACTTTTCTCTTCCCTGCCTTAACACTGATGTTCAGTAATGTATTCAACCAGACAAATGTGTCATCATATAATCTTTGATCCTTCCTTCATGCGAAGGGTTAATGGAGAAATATGTTTGGGGTATTTGTATAGACACTTTGGTTAAGGAGTC
CDS Sequence
Protein Sequence