Gene Details:
- Gene ID: AT5G14420
- Gene Symbol: RGLG2
- Gene Name: RING domain ligase2
- Description: RING domain ligase2;(source:Araport11)
- TAIR Accession: locus:2145643
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0000036 — leaf vascular system — sistema vascular de la hoja (Spanish, exact), 葉維管系 (Japanese, exact), leaf venation (related)
- PO:0000332 — shoot epidermal pavement cell — célula del pavimento epidermal (Spanish, exact), 表皮被蓋細胞 (Japanese, exact)
- PO:0003011 — root vascular system — sistema vascular de la raíz (Spanish, exact), 根維管系 (Japanese, exact), root vein (related)
- PO:0020030 — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
- PO:0000084 — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)
Gene Ontology:
- GO:0004842 — enables — ubiquitin-protein transferase activity
- GO:0005515 — enables — protein binding
- GO:0005634 — located in — nucleus
- GO:0061630 — enables — ubiquitin protein ligase activity
- GO:0009690 — acts upstream of or within — cytokinin metabolic process
- GO:0009850 — acts upstream of or within — auxin metabolic process
- GO:0005634 — is active in — nucleus
- GO:0080148 — acts upstream of or within — negative regulation of response to water deprivation
- GO:0005886 — located in — plasma membrane
Germplasm Phenotype:
- CS24985 — No visible phenotype.
- axr1-12 rglg1 rglg2 — The triple mutant had an approximately additive phenotype. They were bushy and displayed the reduced fertility of axr1-12 plants.
- rglg1 rglg2 — Although the overall growth of young rglg1 rglg2 seedlings closely resembled that of wild-type plants, the early rosette leaves were more elongated and darker. These early rosette leaves senesce after ~4 to 6 weeks of growth. The first emerging shoots were short and terminated by one or few flowers yielding fertile siliques. Later developing shoots were longer. Another difference between the wild type and the rglg1 rglg2 mutant was leaf shape and architecture. Mutant leaves were more oblong and often asymmetric and had reduced numbers of veins. Whereas leaf initials in the wild type formed a spiral, with an ~137.5° angle between leaf initials, the rglg1 rglg2 mutant formed two initials opposite to each other and then switched by 90° to form the next pair of opposing leaves. Thus, rglg1 rglg2 plants often had a decussate pattern of leaf mergence, instead of the spiral pattern of wild-type Arabidopsis. Additional phenotypes of aerial plant parts, in particular an increased cell size.
- rglg1 rglg2 — Auxin (IAA) concentration was almost twofold lower in roots of 2-week-old mutant seedlings compared to wild type.
- rglg1 rglg2 — Changes in the concentrations of cytokinin nucleotides and conjugates.
- rglg1 rglg2 — Impaired circadian clock function.
- rglg1 rglg2 — Roots of wild-type and mutant plants were similar in spite of a moderate (14%) reduction in the main root length of plantlets grown on plates.
- rglg1 rglg2 — The double mutant showed a complete loss of apical dominance, lacking normal dominance of the central apical meristem, and the development of a central inflorescence. All double mutants grown to maturity had >30 shoots of comparable length.
- tir3-1 RGLG1:rglg1 rglg2 — Showed some phenotypic similarity with tir3-1 axr1-12 plants, being more bushy than tir3-1 plants and often producing siliques in bundles.
- tir3-1 RGLG1:rglg1 rglg2 — The triple homozygous progeny has low viability, accumulated anthocyanin, and all plants died before shoot emergence.
Function-related keywords:
- root , leaf vascular system , shoot epidermal pavement cell , root vascular system , root , cotyledon , plant sperm cell
Literature:
- Evaluation and classification of RING-finger domains encoded by the Arabidopsis genome. DOI: 10.1186/gb-2002-3-4-research0016 ; PMID: 11983057
- Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote. DOI: 10.1074/jbc.M307321200 ; PMID: 12912986
- Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. DOI: 10.1242/jcs.00715 ; PMID: 12953064
- Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. DOI: 10.1104/pp.104.052423 ; PMID: 15644464
- Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis. DOI: 10.1104/pp.105.067983 ; PMID: 16339806
- Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis. DOI: 10.1105/tpc.107.052035 ; PMID: 17586653
- A lysine-63-linked ubiquitin chain-forming conjugase, UBC13, promotes the developmental responses to iron deficiency in Arabidopsis roots. DOI: 10.1111/j.1365-313X.2010.04150.x ; PMID: 20113438
- Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and negatively regulates the plant drought stress response. DOI: 10.1104/pp.111.189738 ; PMID: 22095047
- Functional characterization of an abiotic stress-inducible transcription factor AtERF53 in Arabidopsis thaliana. DOI: 10.1007/s11103-013-0054-z ; PMID: 23625358
- Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA. DOI: 10.1105/tpc.16.00364 ; PMID: 27577789
- CORRECTION. Arabidopsis RGLG2, Functioning as a RING E3 Ligase, Interacts with AtERF53 and Negatively Regulates the Plant Drought Stress Response. DOI: 10.1104/pp.16.00025 ; PMID: 26826724
- RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis. DOI: 10.1111/jipb.13019 ; PMID: 32970364
- Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote. DOI: 10.1074/jbc.M307321200 ; PMID: 12912986
- Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. DOI: 10.1104/pp.104.052423 ; PMID: 15644464
- Repertoire of plant RING E3 ubiquitin ligases revisited: New groups counting gene families and single genes. DOI: 10.1371/journal.pone.0203442 ; PMID: 30169501
Sequences:
cDNA Sequence
- >AT5G14420.1
AGGTATAGGTAAATTGCATCTTCTAATAATGTCAGAATCTTAAACAAAGTTTCTGCTTTTTTTTTCTGGGGTTTCATGAATATACTCTAAAGGCTAAATCTTTGTTTTGCAGGGTACGGAACTGACCTGAGAGGTTTGATTTGAATCTAAAAAGGTTTAGTTTTTTGTTAAGTTTGATGGGGACAGGGAATTCTAAAGAAAACTGGAGACAGTCATCGTTTAGGTCAACTTCTGCTTCATCAGCATCACCATCTTCATCTTCATGGGCTTCTCAACAAAGTTATCCTCAGTATGGTGCAGAAAGCTATAATTACCCTCCTCCACCTTCTTATGCCCAACCTCCTGAGTATACGCAACCTCCTCCTCCTTTATATAGTACTCAGCCTTACTCTGCTCCGTCTTATTCTGCACCGCCTTCTCAAAGTTATGGTAGTGATAATAAGAAGAGGTTGGAGCGCAAGTATTCGAAAATTTCTGATGATTACTCTTCTTTGGAGCAGGTGACGGAGGCTCTTGCACGGGCGGGTCTAGAATCTTCAAATCTCATCGTTGGTATTGATTTCACTAAGAGTAACGAATGGACAGGGGCCAGGTCCTTCAATAGGAAAAGCTTACACTTCATAGGCAGTAGTCCCAATCCTTATGAACAAGCGATAACTATCATAGGAAGAACCTTAGCCGCCTTTGACGAGGACAACTTGATTCCATGTTATGGTTTTGGAGATGCATCAACACATGATCAAGACGTGTTCAGTTTCAATTCAGAGGATAGATTCTGTAATGGATTTGAGGAAGTACTTTCCCGGTATAAGGAGATCGTGCCTCAACTTAAGCTTGCAGGGCCAACCTCCTTTGCTCCGATCATCGATATGGCCATGACCATTGTTGAGCAGAGTGGTGGGCAATATCACGTCCTAGTGATAATAGCAGATGGGCAGGTTACAAGAAGTGTTGACACGGAAAATGGACAGTTAAGTCCACAAGAACAGAAGACGGTAGATGCGATCGTGCAAGCGAGTAAGCTTCCTCTATCAATCGTCTTAGTCGGGGTGGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGATAATATTCCGGCCAGAGCGTTTGATAACTTCCAATTCGTGAACTTCACAGAGATCATGGCAAAGAACAAAGCTCAATCCTTGAAGGAAACAGAGTTTGCCCTTTCTGCTCTCATGGAGATTCCTCAACAATACAAGGCGACAATAGAGCTTAACCTTTTAGGAAGAAGAAATGGATATATCCCGGAGAGATTCCCTCTTCCGCCTCCAATGCGCGGTGGATCATCGTCCTACAACAGTCCAAAGCCTTCACGTCTTCCAAGTTTCAAACCGAGTGTTCCTCCTCATCCAACTGAAGGCTATCACGTAAGGTCTAGTCCCGTCCCACCCCCTACAAGCTCTGCTTCTGATAACCAGCTATGTCCGATTTGTTTGAGCAACCCGAAAGACATGGCCTTTGGTTGTGGCCATCAGACTTGTTGCGAGTGTGGACCAGATCTTCAAATGTGTCCGATTTGCCGTGCACCAATCCAGACAAGAATAAAGCTCTACTAACAAACAAGTAGACCAGACATCGACGACCCTTCTCTCTCTTTCATCTTCTTTCAACTTCTTTTGGGAATTTAGTTTGAATCTAATCACTGAAATTTCTCCGAGAAAGATCGGATCTTTTGTATAAAGAAAAAAACAATGCTTGTTCGTTTACTCACTTTCCTAACTTCATCTTGTCCCAAAACCTTTGACTTCTTTCTTTCTTTCTATCTTTCTTCTTTGCTTGGAGTAAAAAAATTGTTATTCATGTGCCCA - >AT5G14420.4
AGGTATAGGTAAATTGCATCTTCTAATAATGTCAGAATCTTAAACAAAGTTTCTGCTTTTTTTTTCTGGGGTTTCATGAATATACTCTAAAGGCTAAATCTTTGTTTTGCAGGGTACGGAACTGACCTGAGAGGTTTGATTTGAATCTAAAAAGGTTTAGTTTTTTGTTAAGTTTGATGGGGACAGGGAATTCTAAAGAAAACTGGAGACAGTCATCGTTTAGGTCAACTTCTGCTTCATCAGCATCACCATCTTCATCTTCATGGGCTTCTCAACAAAGTTATCCTCAGTATGGTGCAGAAAGCTATAATTACCCTCCTCCACCTTCTTATGCCCAACCTCCTGAGTATACGCAACCTCCTCCTCCTTTATATAGTACTCAGCCTTACTCTGCTCCGTCTTATTCTGCACCGCCTTCTCAAAGTTATGGTAGTGATAATAAGAAGAGGTTGGAGCGCAAGTATTCGAAAATTTCTGATGATTACTCTTCTTTGGAGCAGGTGACGGAGGCTCTTGCACGGGCGGGTCTAGAATCTTCAAATCTCATCGTTGGTATTGATTTCACTAAGAGTAACGAATGGACAGGGGCCAGGTCCTTCAATAGGAAAAGCTTACACTTCATAGGCAGTAGTCCCAATCCTTATGAACAAGCGATAACTATCATAGGAAGAACCTTAGCCGCCTTTGACGAGGACAACTTGATTCCATGTTATGGTTTTGGAGATGCATCAACACATGATCAAGACGTGTTCAGTTTCAATTCAGAGGATAGATTCTGTAATGGATTTGAGGAAGTACTTTCCCGGTATAAGGAGATCGTGCCTCAACTTAAGCTTGCAGGGCCAACCTCCTTTGCTCCGATCATCGATATGGCCATGACCATTGTTGAGCAGAGTGGTGGGCAATATCACGTCCTAGTGATAATAGCAGATGGGCAGGTTACAAGAAGTGTTGACACGGAAAATGGACAGTTAAGTCCACAAGAACAGAAGACGGTAGATGCGATCGTGCAAGCGAGTAAGCTTCCTCTATCAATCGTCTTAGTCGGGGTGGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGATAATATTCCGGCCAGAGCGTTTGATAACTTCCAATTCGTGAACTTCACAGAGATCATGGCAAAGAACAAAGCTCAATCCTTGAAGGAAACAGAGTTTGCCCTTTCTGCTCTCATGGAGATTCCTCAACAATACAAGGCGACAATAGAGCTTAACCTTTTAGGAAGAAGAAATGGATATATCCCGGAGAGATTCCCTCTTCCGCCTCCAATGCGCGGTGGATCATCGTCCTACAACAGTCCAAAGCCTTCACGTCTTCCAAGTTTCAAACCGAGTGTTCCTCCTCATCCAACTGAAGGCTATCACGTAAGGTCTAGTCCCGTCCCACCCCCTACAAGCTCTGCTTCTGATAACCAGCTATGTCCGATTTGTTTGAGCAACCCGAAAGACATGGCCTTTGGTTGTGGCCATCAGACTTGTTGCGAGTGTGGACCAGATCTTCAAATGTGTCCGATTTGCCGTGCACCAATCCAGACAAGAATAAAGCTCTACTAACAAACAAGTAGACCAGACATCGACGACCCTTCTCTCTCTTTCATCTTCTTTCAACTTCTTTTGGGAATTTAGTTTGAATCTAATCACTGAAATTTCTCCGAGAAAGATCGGATCTTTTGTATAAAGAAAAAAACAATGCTTGTTCGTTTACTCACTTTCCTAACTTCATCTTGTCCCAAAACCTTTGACTTCTTTCTTTCTTTCTATCTTTCTTCTTTGCTTGGAGTAAAAAAATTGTTATTCATGTGCCCA - >AT5G14420.2
AGGTATAGGTAAATTGCATCTTCTAATAATGTCAGAATCTTAAACAAAGTTTCTGCTTTTTTTTTCTGGGGTTTCATGAATATACTCTAAAGGCTAAATCTTTGTTTTGCAGGGTACGGAACTGACCTGAGAGGTTTGATTTGAATCTAAAAAGGTTTAGTTTTTTGTTAAGTTTGATGGGGACAGGGAATTCTAAAGAAAACTGGAGACAGTCATCGTTTAGGTCAACTTCTGCTTCATCAGCATCACCATCTTCATCTTCATGGGCTTCTCAACAAAGTTATCCTCAGTATGGTGCAGAAAGCTATAATTACCCTCCTCCACCTTCTTATGCCCAACCTCCTGAGTATACGCAACCTCCTCCTCCTTTATATAGTACTCAGCCTTACTCTGCTCCGTCTTATTCTGCACCGCCTTCTCAAAGTTATGGTAGTGATAATAAGAAGAGGTTGGAGCGCAAGTATTCGAAAATTTCTGATGATTACTCTTCTTTGGAGCAGGTGACGGAGGCTCTTGCACGGGCGGGTCTAGAATCTTCAAATCTCATCGTTGGTATTGATTTCACTAAGAGTAACGAATGGACAGGGGCCAGGTCCTTCAATAGGAAAAGCTTACACTTCATAGGCAGTAGTCCCAATCCTTATGAACAAGCGATAACTATCATAGGAAGAACCTTAGCCGCCTTTGACGAGGACAACTTGATTCCATGTTATGGTTTTGGAGATGCATCAACACATGATCAAGACGTGTTCAGTTTCAATTCAGAGGATAGATTCTGTAATGGATTTGAGGAAGTACTTTCCCGGTATAAGGAGATCGTGCCTCAACTTAAGCTTGCAGGGCCAACCTCCTTTGCTCCGATCATCGATATGGCCATGACCATTGTTGAGCAGAGTGGTGGGCAATATCACGTCCTAGTGATAATAGCAGATGGGCAGGTTACAAGAAGTGTTGACACGGAAAATGGACAGTTAAGTCCACAAGAACAGAAGACGGTAGATGCGATCGTGCAAGCGAGTAAGCTTCCTCTATCAATCGTCTTAGTCGGGGTGGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGATAATATTCCGGCCAGAGCGTTTGATAACTTCCAATTCGTGAACTTCACAGAGATCATGGCAAAGAACAAAGCTCAATCCTTGAAGGAAACAGAGTTTGCCCTTTCTGCTCTCATGGAGATTCCTCAACAATACAAGGCGACAATAGAGCTTAACCTTTTAGGAAGAAGAAATGGATATATCCCGGAGAGATTCCCTCTTCCGCCTCCAATGCGCGGTGGATCATCGTCCTACAACAGTCCAAAGCCTTCACGTCTTCCAAGTTTCAAACCGAGTGTTCCTCCTCATCCAACTGAAGGCTATCACGTAAGGTCTAGTCCCGTCCCACCCCCTACAAGCTCTGCTTCTGATAACCAGCTATGTCCGATTTGTTTGAGCAACCCGAAAGACATGGCCTTTGGTTGTGGCCATCAGACTTGTTGCGAGTGTGGACCAGATCTTCAAATGTGTCCGATTTGCCGTGCACCAATCCAGACAAGAATAAAGCTCTACTAACAAACAAGTAGACCAGACATCGACGACCCTTCTCTCTCTTTCATCTTCTTTCAACTTCTTTTGGGAATTTAGTTTGAATCTAATCACTGAAATTTCTCCGAGAAAGATCGGATCTTTTGTATAAAGAAAAAAACAATGCTTGTTCGTTTACTCACTTTCCTAACTTCATCTTGTCCCAAAACCTTTGACTTCTTTCTTTCTTTCTATCTTTCTTCTTTGCTTGGAGTAAAAAAATTGTTATTCATGTGCCCA - >AT5G14420.3
AGGTATAGGTAAATTGCATCTTCTAATAATGTCAGAATCTTAAACAAAGTTTCTGCTTTTTTTTTCTGGGGTTTCATGAATATACTCTAAAGGCTAAATCTTTGTTTTGCAGGGTACGGAACTGACCTGAGAGGTTTGATTTGAATCTAAAAAGGTTTAGTTTTTTGTTAAGTTTGATGGGGACAGGGAATTCTAAAGAAAACTGGAGACAGTCATCGTTTAGGTCAACTTCTGCTTCATCAGCATCACCATCTTCATCTTCATGGGCTTCTCAACAAAGTTATCCTCAGTATGGTGCAGAAAGCTATAATTACCCTCCTCCACCTTCTTATGCCCAACCTCCTGAGTATACGCAACCTCCTCCTCCTTTATATAGTACTCAGCCTTACTCTGCTCCGTCTTATTCTGCACCGCCTTCTCAAAGTTATGGTAGTGATAATAAGAAGAGGTTGGAGCGCAAGTATTCGAAAATTTCTGATGATTACTCTTCTTTGGAGCAGGTGACGGAGGCTCTTGCACGGGCGGGTCTAGAATCTTCAAATCTCATCGTTGGTATTGATTTCACTAAGAGTAACGAATGGACAGGGGCCAGGTCCTTCAATAGGAAAAGCTTACACTTCATAGGCAGTAGTCCCAATCCTTATGAACAAGCGATAACTATCATAGGAAGAACCTTAGCCGCCTTTGACGAGGACAACTTGATTCCATGTTATGGTTTTGGAGATGCATCAACACATGATCAAGACGTGTTCAGTTTCAATTCAGAGGATAGATTCTGTAATGGATTTGAGGAAGTACTTTCCCGGTATAAGGAGATCGTGCCTCAACTTAAGCTTGCAGGGCCAACCTCCTTTGCTCCGATCATCGATATGGCCATGACCATTGTTGAGCAGAGTGGTGGGCAATATCACGTCCTAGTGATAATAGCAGATGGGCAGGTTACAAGAAGTGTTGACACGGAAAATGGACAGTTAAGTCCACAAGAACAGAAGACGGTAGATGCGATCGTGCAAGCGAGTAAGCTTCCTCTATCAATCGTCTTAGTCGGGGTGGGGGATGGACCTTGGGACATGATGAGGGAATTTGACGATAATATTCCGGCCAGAGCGTTTGATAACTTCCAATTCGTGAACTTCACAGAGATCATGGCAAAGAACAAAGCTCAATCCTTGAAGGAAACAGAGTTTGCCCTTTCTGCTCTCATGGAGATTCCTCAACAATACAAGGCGACAATAGAGCTTAACCTTTTAGGAAGAAGAAATGGATATATCCCGGAGAGATTCCCTCTTCCGCCTCCAATGCGCGGTGGATCATCGTCCTACAACAGTCCAAAGCCTTCACGTCTTCCAAGTTTCAAACCGAGTGTTCCTCCTCATCCAACTGAAGGCTATCACGTAAGGTCTAGTCCCGTCCCACCCCCTACAAGCTCTGCTTCTGATAACCAGCTATGTCCGATTTGTTTGAGCAACCCGAAAGACATGGCCTTTGGTTGTGGCCATCAGACTTGTTGCGAGTGTGGACCAGATCTTCAAATGTGTCCGATTTGCCGTGCACCAATCCAGACAAGAATAAAGCTCTACTAACAAACAAGTAGACCAGACATCGACGACCCTTCTCTCTCTTTCATCTTCTTTCAACTTCTTTTGGGAATTTAGTTTGAATCTAATCACTGAAATTTCTCCGAGAAAGATCGGATCTTTTGTATAAAGAAAAAAACAATGCTTGTTCGTTTACTCACTTTCCTAACTTCATCTTGTCCCAAAACCTTTGACTTCTTTCTTTCTTTCTATCTTTCTTCTTTGCTTGGAGTAAAAAAATTGTTATTCATGTGCCCA
CDS Sequence
Protein Sequence