Gene Details:
- Gene ID: AT5G13120
- Gene Symbol: ATCYP20-2, CYP20-2, Pnsl5
- Gene Name: ARABIDOPSIS THALIANA CYCLOPHILIN 20-2, cyclophilin 20-2, Photosynthetic NDH subcomplex L 5
- Description: cyclophilin 20-2;(source:Araport11)
- TAIR Accession: locus:2179822
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
Gene Ontology:
- GO:0006457 — involved in — protein folding
- GO:0009507 — located in — chloroplast
- GO:0003755 — enables — peptidyl-prolyl cis-trans isomerase activity
- GO:0009535 — located in — chloroplast thylakoid membrane
- GO:0005515 — enables — protein binding
- GO:0009534 — located in — chloroplast thylakoid
- GO:0043424 — enables — protein histidine kinase binding
- GO:0009533 — located in — chloroplast stromal thylakoid
- GO:0009543 — located in — chloroplast thylakoid lumen
- GO:0009536 — located in — plastid
- GO:0005737 — is active in — cytoplasm
- GO:0016018 — enables — cyclosporin A binding
- GO:0009579 — located in — thylakoid
- GO:0031977 — located in — thylakoid lumen
Germplasm Phenotype:
- SALK_024971C — This mutant had a normal phenotype when grown under standard conditions. The chl/leaf area ratio in this mutant and WT plants were 20.1+/- 0.8 and 19.0 +/- 0.7 respectively. The average chl a/b ratios in this mutant and WT plants were 3.7 +/- 0.0 and 3.6 +/- 0.1 respectively. The levels of representative proteins from various complexes including photosystem (PS)I, PSII, and cytb6f were not altered cyp20-2 thylakoid membranes. However, there was a ~20% reduction in the amount of NDHH, a subunit of the NAD(P)H dehydrogenase complex. There was a slight increase in the photochemical efficiency of PSII in constant growth light conditions and following a two-hour exposure to high light. An marker of NAD(P)H dehydrogenase-mediated reduction of plastoquinone in the dark, measured through the post-illumination rise in chl fluorescence, was also moderately decreased in this mutant.
- salk_009952 (cyp20-2) — This mutant had a normal phenotype when grown under standard conditions. The chl/leaf area ratio in this mutant and WT plants were 18.1+/- 0.6 and 19.0 +/- 0.7 respectively. The average chl a/b ratios in this mutant and WT plants were 3.7 +/- 0.0 and 3.6 +/- 0.1 respectively. The levels of representative proteins from various complexes including photosystem (PS)I, PSII, and cytb6f were not altered cyp20-2 thylakoid membranes. However, there was a ~20% reduction in the amount of NDHH, a subunit of the NAD(P)H dehydrogenase complex. There was a slight increase in the photochemical efficiency of PSII in constant growth light conditions. An marker of NAD(P)H dehydrogenase-mediated reduction of plastoquinone in the dark, measured through the post-illumination rise in chl fluorescence, was also moderately decreased in this mutant.
Function-related keywords:
Literature:
- Proteome map of the chloroplast lumen of Arabidopsis thaliana. DOI: 10.1074/jbc.M108575200 ; PMID: 11719511
- Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction. DOI: 10.1105/tpc.010304 ; PMID: 11826309
- Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. DOI: 10.1074/jbc.M308435200 ; PMID: 14576160
- The Arabidopsis cyclophilin gene family. DOI: 10.1104/pp.103.022160 ; PMID: 15051864
- Immunophilins and parvulins. Superfamily of peptidyl prolyl isomerases in Arabidopsis. DOI: 10.1104/pp.103.031005 ; PMID: 15047905
- New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy. DOI: 10.1074/jbc.M406763200 ; PMID: 15322131
- Profound redox sensitivity of peptidyl-prolyl isomerase activity in Arabidopsis thylakoid lumen. DOI: 10.1016/j.febslet.2006.05.054 ; PMID: 16765949
- The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show and long-term exposure to low temperature. DOI: 10.1111/j.1365-313X.2006.02821.x ; PMID: 16923014
- Sorting signals, N-terminal modifications and abundance of the chloroplast proteome. DOI: 10.1371/journal.pone.0001994 ; PMID: 18431481
- Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. DOI: 10.1104/pp.108.121038 ; PMID: 18650403
- Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature for nuclear-encoded subunits. DOI: 10.1093/pcp/pcr098 ; PMID: 21785130
- The cyclophilin CYP20-2 modulates the conformation of BRASSINAZOLE-RESISTANT1, which binds the promoter of FLOWERING LOCUS D to regulate flowering in Arabidopsis. DOI: 10.1105/tpc.113.110296 ; PMID: 23897924
- Proteome map of the chloroplast lumen of Arabidopsis thaliana. DOI: 10.1074/jbc.M108575200 ; PMID: 11719511
- Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction. DOI: 10.1105/tpc.010304 ; PMID: 11826309
- The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions. DOI: 10.1016/j.cub.2004.02.039 ; PMID: 15028209
- New functions of the thylakoid membrane proteome of Arabidopsis thaliana revealed by a simple, fast, and versatile fractionation strategy. DOI: 10.1074/jbc.M406763200 ; PMID: 15322131
- Sorting signals, N-terminal modifications and abundance of the chloroplast proteome. DOI: 10.1371/journal.pone.0001994 ; PMID: 18431481
- Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling. DOI: 10.1016/j.jprot.2017.06.004 ; PMID: 28627464
Sequences:
cDNA Sequence
- >AT5G13120.2
TCACTCGCTCATCGTCACCTTCACCTTCACCTACCATTTTTGCCAACAAACGCGAGCAAGAGTTTTGAGCTCCAATGGCGACCTTGTCGATGACTCTGTCGCTCTCAGCTCCTCCACGAAGACTTTCTCCGATCAATACCTCCGCTTTCACATCTACCTCTTTCCGATTGCGCACCAAATCTTCCTTCGATTCAATCTCGTTCTCGTCTTCAACTCCCTTCTCCGCCTCATCGCTCCTCCTTCACACGTCTTATACCAAAAGAAACCACCGCTGTTTCTCTGTTCAATCCAATGCTGAGGTGGTTACTGAACCGCAATCGAAAATAACACACAAGGTGTATTTCGATATAAGTGTTGGCAATCCAGTGGGGAAGTTAGCTGGGAGGATTGTTATTGGATTGTATGGTGATGATGTTCCCCAAACTGTGGAGAACTTTCGTGCTCTATGCACAGGTGAAAAGGGATTTGGCTACAAGGGTTCTACATTCCATCGTGTCATCAGAGATTTCATGATTCAGGGAGGAGACTTTGAGAAGGGCAATGGAACTGGAGGTAAAAGTGTATATGGCCGTACATTCAAGGATGAGAATTTCAAGTTATCCCATGTCGGACCAGGAGTTCTAAGTATGGCAAATGCTGGCCCTAATACAAATGGAAGTCAGTTCTTTATCTGCACTATTAAGACGTCATGGTTGGACGGAAGACATGTGGTGTTCGGGCAAGTAATAGAAGGAATGGAAGTGGTGAAGCTGATAGAGGAACAAGAGACAGACAGAGGAGACCGTCCCAGAAAGAAAGTGGTGATAGCAGACTGTGGTCAACTTCCAATGTCTGAAGCTTAAAAGTAAGATCATTTAACCAAAATCTTGTAGACATGAGATGGAGCTTGTTTTTGCATTTTACAACCCTTTCTTTTGTGTAATGGATTTGTATCCCCTTCTTCCAAAAATTTAAGAAAGAAATAGTATTCCGCTGCCGGGTGTTGAACTCATATTTCATTTCATTGGTTTGTTTCGAAATTAACCATTGTGTTATAG - >AT5G13120.1
TCACTCGCTCATCGTCACCTTCACCTTCACCTACCATTTTTGCCAACAAACGCGAGCAAGAGTTTTGAGCTCCAATGGCGACCTTGTCGATGACTCTGTCGCTCTCAGCTCCTCCACGAAGACTTTCTCCGATCAATACCTCCGCTTTCACATCTACCTCTTTCCGATTGCGCACCAAATCTTCCTTCGATTCAATCTCGTTCTCGTCTTCAACTCCCTTCTCCGCCTCATCGCTCCTCCTTCACACGTCTTATACCAAAAGAAACCACCGCTGTTTCTCTGTTCAATCCAATGCTGAGGTGGTTACTGAACCGCAATCGAAAATAACACACAAGGTGTATTTCGATATAAGTGTTGGCAATCCAGTGGGGAAGTTAGCTGGGAGGATTGTTATTGGATTGTATGGTGATGATGTTCCCCAAACTGTGGAGAACTTTCGTGCTCTATGCACAGGTGAAAAGGGATTTGGCTACAAGGGTTCTACATTCCATCGTGTCATCAGAGATTTCATGATTCAGGGAGGAGACTTTGAGAAGGGCAATGGAACTGGAGGTAAAAGTGTATATGGCCGTACATTCAAGGATGAGAATTTCAAGTTATCCCATGTCGGACCAGGAGTTCTAAGTATGGCAAATGCTGGCCCTAATACAAATGGAAGTCAGTTCTTTATCTGCACTATTAAGACGTCATGGTTGGACGGAAGACATGTGGTGTTCGGGCAAGTAATAGAAGGAATGGAAGTGGTGAAGCTGATAGAGGAACAAGAGACAGACAGAGGAGACCGTCCCAGAAAGAAAGTGGTGATAGCAGACTGTGGTCAACTTCCAATGTCTGAAGCTTAAAAGTAAGATCATTTAACCAAAATCTTGTAGACATGAGATGGAGCTTGTTTTTGCATTTTACAACCCTTTCTTTTGTGTAATGGATTTGTATCCCCTTCTTCCAAAAATTTAAGAAAGAAATAGTATTCCGCTGCCGGGTGTTGAACTCATATTTCATTTCATTGGTTTGTTTCGAAATTAACCATTGTGTTATAG
CDS Sequence
Protein Sequence