Gene Details:

• Gene ID: AT5G10490
• Gene Symbol: MSL2
• Gene Name: MSCS-like 2
• Description: MSCS-like 2;(source:Araport11)
• TAIR Accession: locus:2142414
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT5G10490.1     AT5G10490.2     AT5G10490.3

Plant Ontology Annotations:

• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009049  — inflorescence — inflorescencia (Spanish, exact), 花序 (Japanese, exact), Triticeae spike (narrow)
• PO:0000084  — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)

Gene Ontology:

• GO:0055085  — involved in — transmembrane transport
• GO:0009507  — located in — chloroplast
• GO:0009657  — acts upstream of or within — plastid organization
• GO:0005886  — located in — plasma membrane
• GO:0009526  — located in — plastid envelope
• GO:0010020  — acts upstream of or within — chloroplast fission
• GO:0005216  — enables — monoatomic ion channel activity

Germplasm Phenotype:

• CS69609  — No visible phenotype
• CS69610  — Variegated leaves, enlarged plastids. Partial infertility and reduced seed set, likely a result of a fragment of the APETALA3 gene present in the T-DNA construct.
• CS69611  — Slightly smaller than wild type. Young leaves exhibit notched edges and a rumpled surface.
• CS69612  — Small stature, variegated leaves and ruffled leaf margins, apical callus production after long periods of growth on solid media, enlarged plastids.
• CS73251  — Slightly smaller than wild type Col-0, phenotypically similar to msl2-3 (CS69611). Young leaves exhibit notched edges and a rumpled surface.
• CS73252  — In addition to the rumpled leaf surface reminiscent of msl2 mutants, the double mutant shows variegated leaves and an even more significant growth deficit.
• CS73253  — In addition to the rumpled leaf surface reminiscent of msl2 mutants, the double mutant shows variegated leaves and an even more significant growth deficit.
• msl2-1 msl3-1  — Variegated leaf phenotype; light green sectors most dramatic in cauline leaves, but also visible in most rosette leaves. Microscopic observation revealed enlarged chloroplasts in 20% of mesophyll cells in cauline leaves. Leaf tissue had larger air spaces between cells compared to the wild type, and all cells, including epidermal, palisade and mesophyll cells were larger that in the wild type. Under TEM, ultrastructure of enlarged chloroplasts had lumpy appearance of thylakoid membranes. Plastids of the pavement cells in the leaf epidermis in leaves and cotyledons and also in the root epidermal cells were much larger, spherical, stromules were not observed. Beside these characteristics, overall appearance of double mutant plants was similar to the wild type plants.

Function-related keywords:

• root ,  root ,  vascular leaf ,  inflorescence ,  plant sperm cell

Literature:

• MscS-like proteins control plastid size and shape in Arabidopsis thaliana.   DOI:  10.1016/j.cub.2005.11.044 ;  PMID:  16401419
• Comparative transcriptomics of Arabidopsis sperm cells.   DOI:  10.1104/pp.108.125229 ;  PMID:  18667720
• Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.   DOI:  10.1104/pp.108.126375 ;  PMID:  18775970
• Genome-wide identification and evolutionary analysis of Arabidopsis sm genes family.   DOI:  10.1080/07391102.2011.10508593 ;  PMID:  21142222
• Two mechanosensitive channel homologs influence division ring placement in Arabidopsis chloroplasts.   DOI:  10.1105/tpc.111.088112 ;  PMID:  21810996
• Mechanosensitive channels protect plastids from hypoosmotic stress during normal plant growth.   DOI:  10.1016/j.cub.2012.01.027 ;  PMID:  22326022
• Plastid osmotic stress activates cellular stress responses in Arabidopsis.   DOI:  10.1104/pp.114.236620 ;  PMID:  24676856
• RNA Sequencing Analysis of the msl2msl3, crl, and ggps1 Mutants Indicates that Diverse Sources of Plastid Dysfunction Do Not Alter Leaf Morphology Through a Common Signaling Pathway.   DOI:  10.3389/fpls.2015.01148 ;  PMID:  26734046
• Plastid osmotic stress influences cell differentiation at the plant shoot apex.   DOI:  10.1242/dev.136234 ;  PMID:  27510974
• Genetic and physical interactions between the organellar mechanosensitive ion channel homologs MSL1, MSL2, and MSL3 reveal a role for inter-organellar communication in plant development.   DOI:  10.1002/pld3.124 ;  PMID:  31245767
• Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.   DOI:  10.1371/journal.pone.0001994 ;  PMID:  18431481
• Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.   DOI:  10.1371/journal.pone.0001994 ;  PMID:  18431481

Sequences: