Gene Details:

  • Gene ID: AT5G10140
  • Gene Symbol: AGL25, FLC, FLF, RSB6
  • Gene Name: AGAMOUS-like 25, FLOWERING LOCUS C, FLOWERING LOCUS F, REDUCED STEM BRANCHING 6
  • Description: K-box region and MADS-box transcription factor family protein;(source:Araport11)
  • TAIR Accession: locus:2184118
  • Genome: Araport11_genome_release
  • Species: Arabidopsis thaliana

Transcripts:

Plant Ontology Annotations:

  • PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
  • PO:0009013  — portion of meristem tissue — meristem (exact), meristema (Spanish, exact), meristematic tissue (exact), 分裂組織 (Japanese, exact)
  • PO:0000025  — root tip — punta de la raíz (Spanish, exact), 根端 (Japanese, exact)
  • PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
  • PO:0009010  — seed — semilla (Spanish, exact), 種子 (Japanese, exact), pyrene (narrow), diaspore (broad)

Gene Ontology:

  • GO:2000028  — involved in — regulation of photoperiodism, flowering
  • GO:0000976  — enables — transcription cis-regulatory region binding
  • GO:0003700  — enables — DNA-binding transcription factor activity
  • GO:0005634  — located in — nucleus
  • GO:0045944  — involved in — positive regulation of transcription by RNA polymerase II
  • GO:0005515  — enables — protein binding
  • GO:0000977  — enables — RNA polymerase II transcription regulatory region sequence-specific DNA binding
  • GO:0010048  — acts upstream of or within — vernalization response
  • GO:0000978  — enables — RNA polymerase II cis-regulatory region sequence-specific DNA binding
  • GO:0006357  — involved in — regulation of transcription by RNA polymerase II
  • GO:0009910  — acts upstream of or within — negative regulation of flower development
  • GO:0009910  — involved in — negative regulation of flower development
  • GO:0000981  — enables — DNA-binding transcription factor activity, RNA polymerase II-specific
  • GO:0042752  — acts upstream of or within — regulation of circadian rhythm
  • GO:0046983  — enables — protein dimerization activity
  • GO:0009266  — acts upstream of or within — response to temperature stimulus
  • GO:0032991  — part of — protein-containing complex
  • GO:0009266  — involved in — response to temperature stimulus

Germplasm Phenotype:

  • CS1122  — variation in flowering time, height = 40-45 cm.
  • CS1123  — variation in flowering time, height = 40-45 cm.
  • CS189  — not late flowering alone; when combined with FRI or ld, confers extreme late flowering and strong response to vernalization.
  • CS2110009  — impaired in several environmental pathways and possesses severely reduced flowering responses to changes in photoperiod and ambient temperature
  • CS22574  — After 12 wk vernalization FLC levels increased after 30 d growth post-cold.
  • CS22574  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • CS22574  — After 6 wk vernalization, Lov-1 flowered earlier than Ull-2-5, suggesting that Lov-1 might be insensitive to short periods of cold but then respond quickly after that.
  • CS22574  — FLC levels decreased during cold treatment but then increased significantly after the plants were returned to 23°C.
  • CS22574  — Medium to large size of rosettes with numerous leaves, early leaves have leaf margins irregularly serrated, later leaves have leaf margins almost smooth, late flowering (vernalization at rosette stage may promote earlier flowering), dark-green plants with numerous aerial rosettes, height=56-66 cm.
  • CS22574  — While FLC decreased in Lov-1 plants over a 4-wk cold period, flowering time was not accelerated.
  • CS22579  — Accelerated flowering by 2 weeks vernalization. Flowering time variation between accessions was maximal after 4 wk vernalization.
  • CS22579  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • CS22579  — FLC levels return to a moderately high level after 4 wk vernalization.
  • CS22579  — Large rosettes with numerous oval-shape leaves, elongated petiols, asymmetric and contorted leaf surfaces, leaf margins smooth, late flowering (vernalization at rosette stage may promote earlier flowering), dark-green plants, height=50-60 cm.
  • CS22581  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • CS22581  — Despite responding to 4 wk of cold, Var-2-6 and Ull-2-5 still flowered late after >6 wk of cold; i.e., the vernalization response was relatively slow.
  • CS22581  — Large rosettes with numerous large leaves, leaves with rounded tips, leaf margins irregularly serrated, late flowering (vernalization at rosette stage may promote earlier flowering), dull greyish dark-green plants (slightly more pubescent than normal), height=45-55cm.
  • CS22586  — After 4 wk vernalization, FLC levels had increased (by 30 d after transfer to 23°C) to the same level as nonvernalized plants.
  • CS22586  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • CS22586  — Despite responding to 4 wk of cold, Var-2-6 and Ull-2-5 still flowered late after >6 wk of cold; i.e., the vernalization response was relatively slow.
  • CS22586  — FLC levels decreased during cold treatment but then increased significantly after the plants were returned to 23°C.
  • CS22586  — Flowered extremely late after 8 wk vernalization and required at least 14 wk of cold to fully saturate the vernalization requirement.
  • CS22586  — Large rosettes with numerous leaves, leaf margins serrated, late flowering (vernalization at rosette stage may promote earlier flowering), dark -green plants, height= 42-48 cm.
  • CS22586  — Plants did flower after 4 wk vernalization but considerably later than H51.
  • CS22657  — Large rosettes with numerous leaves, elongated leaves with narrow appearance, leaf margins slightly serrated, late flowering (vernalization at rosette stage may promote earlier flowering), height = 40-50 cm.
  • CS6688  — Accelerated flowering by 2 weeks vernalization. Flowering time variation between accessions was maximal after 4 wk vernalization.
  • CS6688  — After 4 wk vernalization, the repression of FLC expression was maintained.
  • CS6688  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • CS6688  — variation in flowering time, height = 40-45 cm.
  • H51  — Accelerated flowering by 2 weeks vernalization. Flowering time variation between accessions was maximal after 4 wk vernalization.
  • H51  — After 4 wk vernalization, the repression of FLC expression was maintained.
  • H51  — After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
  • SALK_131491  — increased FLC mRNA, late flowering
  • SALK_140021  — reduced FLC mRNA, early flowering
  • agl19-1 flc-6  — The double mutant responded less to vernalization than either single mutant.
  • agl20-101D FRI-Sf2 flc-3  — Extreme acceleration of flowering time.
  • ath1-1 flc-7  — No additive effect on flowering time.
  • flc-1  — Early flowering.
  • flc-2 (FRI-Sf2)  — Wild-type phenotype restored.
  • flc-3  — early flowering in short days
  • flc-3 agl24-2  — Late flowering in both long- and short-day growth conditions but retain a normal response to photoperiod compared to wild type.
  • flc-4 (FRI-Sf2)  — Wild-type phenotype restored.
  • flc-5 (FRI-Sf2)  — Wild-type phenotype restored.
  • flc-8  — Early flowering.
  • hlp1-1/flc-3  — Normal flowering response under long and short days. Loss of flc supresses the phenotype of hlp1-1
  • ldl1-2/ldl2/flc-3  — Late flowering. Flowers later than flc3 single mutant but earlier than ldl1/ldl2 double mutant.
  • p5cs1 p5cs2/P5CS2 flc-8  — Flowering time same as flc-8 single mutant.
  • suf4 flc-3  — Flowering time similar to that of the flc-3 single mutant in both LD and SD conditions.

Literature:

Sequences:

cDNA Sequence
  • >AT5G10140.1
    CGAGAAAAGGAAAAAAAAAAATAGAAAGAGAAAACGCTTAGTATCTCCGGCGACTTGAACCCAAACCTGAGGATCAAATTAGGGCACAAAGCCCTCTCGGAGAGAAGCCATGGGAAGAAAAAAACTAGAAATCAAGCGAATTGAGAACAAAAGTAGCCGACAAGTCACCTTCTCCAAACGTCGCAACGGTCTCATCGAGAAAGCTCGTCAGCTTTCTGTTCTCTGTGACGCATCCGTCGCTCTTCTCGTCGTCTCCGCCTCCGGCAAGCTCTACAGCTTCTCCTCCGGCGATAACCTGGTCAAGATCCTTGATCGATATGGGAAACAGCATGCTGATGATCTTAAAGCCTTGGATCATCAGTCAAAAGCTCTGAACTATGGTTCACACTATGAGCTACTTGAACTTGTGGATAGCAAGCTTGTGGGATCAAATGTCAAAAATGTGAGTATCGATGCTCTTGTTCAACTGGAGGAACACCTTGAGACTGCCCTCTCCGTGACTAGAGCCAAGAAGACCGAACTCATGTTGAAGCTTGTTGAGAATCTTAAAGAAAAGGAGAAAATGCTGAAAGAAGAGAACCAGGTTTTGGCTAGCCAGATGGAGAATAATCATCATGTGGGAGCAGAAGCTGAGATGGAGATGTCACCTGCTGGACAAATCTCCGACAATCTTCCGGTGACTCTCCCACTACTTAATTAGCCACCTTAAATCGGCGGTTGAAATCAAAATCCAAAACATATATAATTATGAAGAAAAAAAAAATAAGATATGTAATTATTCCGCTGATAAGGGCGAGCGTTTGTATATCTTAATACTCTCTCTTTGGCCAAGAGACTTTGTGTGTGATACTTAAGTAGACGGAACTAAGTCAATACTATCTGTTTTAAGACAAAAGGTTGATGAACTTTGTACCTTATTCGTGTGAGAATTGCATCGAGATCTTGAGTGTATGTGTTCTTCACTTCTGTCAAAAACTTGTGTTTGCTTCACAGTGAAGAAGCCTACGGCTTATTTTGCAACAGGGACGTGGCTCTCTCTCTC
  • >AT5G10140.2
    CGAGAAAAGGAAAAAAAAAAATAGAAAGAGAAAACGCTTAGTATCTCCGGCGACTTGAACCCAAACCTGAGGATCAAATTAGGGCACAAAGCCCTCTCGGAGAGAAGCCATGGGAAGAAAAAAACTAGAAATCAAGCGAATTGAGAACAAAAGTAGCCGACAAGTCACCTTCTCCAAACGTCGCAACGGTCTCATCGAGAAAGCTCGTCAGCTTTCTGTTCTCTGTGACGCATCCGTCGCTCTTCTCGTCGTCTCCGCCTCCGGCAAGCTCTACAGCTTCTCCTCCGGCGATAACCTGGTCAAGATCCTTGATCGATATGGGAAACAGCATGCTGATGATCTTAAAGCCTTGGATCATCAGTCAAAAGCTCTGAACTATGGTTCACACTATGAGCTACTTGAACTTGTGGATAGCAAGCTTGTGGGATCAAATGTCAAAAATGTGAGTATCGATGCTCTTGTTCAACTGGAGGAACACCTTGAGACTGCCCTCTCCGTGACTAGAGCCAAGAAGACCGAACTCATGTTGAAGCTTGTTGAGAATCTTAAAGAAAAGGAGAAAATGCTGAAAGAAGAGAACCAGGTTTTGGCTAGCCAGATGGAGAATAATCATCATGTGGGAGCAGAAGCTGAGATGGAGATGTCACCTGCTGGACAAATCTCCGACAATCTTCCGGTGACTCTCCCACTACTTAATTAGCCACCTTAAATCGGCGGTTGAAATCAAAATCCAAAACATATATAATTATGAAGAAAAAAAAAATAAGATATGTAATTATTCCGCTGATAAGGGCGAGCGTTTGTATATCTTAATACTCTCTCTTTGGCCAAGAGACTTTGTGTGTGATACTTAAGTAGACGGAACTAAGTCAATACTATCTGTTTTAAGACAAAAGGTTGATGAACTTTGTACCTTATTCGTGTGAGAATTGCATCGAGATCTTGAGTGTATGTGTTCTTCACTTCTGTCAAAAACTTGTGTTTGCTTCACAGTGAAGAAGCCTACGGCTTATTTTGCAACAGGGACGTGGCTCTCTCTCTC
  • >AT5G10140.4
    CGAGAAAAGGAAAAAAAAAAATAGAAAGAGAAAACGCTTAGTATCTCCGGCGACTTGAACCCAAACCTGAGGATCAAATTAGGGCACAAAGCCCTCTCGGAGAGAAGCCATGGGAAGAAAAAAACTAGAAATCAAGCGAATTGAGAACAAAAGTAGCCGACAAGTCACCTTCTCCAAACGTCGCAACGGTCTCATCGAGAAAGCTCGTCAGCTTTCTGTTCTCTGTGACGCATCCGTCGCTCTTCTCGTCGTCTCCGCCTCCGGCAAGCTCTACAGCTTCTCCTCCGGCGATAACCTGGTCAAGATCCTTGATCGATATGGGAAACAGCATGCTGATGATCTTAAAGCCTTGGATCATCAGTCAAAAGCTCTGAACTATGGTTCACACTATGAGCTACTTGAACTTGTGGATAGCAAGCTTGTGGGATCAAATGTCAAAAATGTGAGTATCGATGCTCTTGTTCAACTGGAGGAACACCTTGAGACTGCCCTCTCCGTGACTAGAGCCAAGAAGACCGAACTCATGTTGAAGCTTGTTGAGAATCTTAAAGAAAAGGAGAAAATGCTGAAAGAAGAGAACCAGGTTTTGGCTAGCCAGATGGAGAATAATCATCATGTGGGAGCAGAAGCTGAGATGGAGATGTCACCTGCTGGACAAATCTCCGACAATCTTCCGGTGACTCTCCCACTACTTAATTAGCCACCTTAAATCGGCGGTTGAAATCAAAATCCAAAACATATATAATTATGAAGAAAAAAAAAATAAGATATGTAATTATTCCGCTGATAAGGGCGAGCGTTTGTATATCTTAATACTCTCTCTTTGGCCAAGAGACTTTGTGTGTGATACTTAAGTAGACGGAACTAAGTCAATACTATCTGTTTTAAGACAAAAGGTTGATGAACTTTGTACCTTATTCGTGTGAGAATTGCATCGAGATCTTGAGTGTATGTGTTCTTCACTTCTGTCAAAAACTTGTGTTTGCTTCACAGTGAAGAAGCCTACGGCTTATTTTGCAACAGGGACGTGGCTCTCTCTCTC
  • >AT5G10140.3
    CGAGAAAAGGAAAAAAAAAAATAGAAAGAGAAAACGCTTAGTATCTCCGGCGACTTGAACCCAAACCTGAGGATCAAATTAGGGCACAAAGCCCTCTCGGAGAGAAGCCATGGGAAGAAAAAAACTAGAAATCAAGCGAATTGAGAACAAAAGTAGCCGACAAGTCACCTTCTCCAAACGTCGCAACGGTCTCATCGAGAAAGCTCGTCAGCTTTCTGTTCTCTGTGACGCATCCGTCGCTCTTCTCGTCGTCTCCGCCTCCGGCAAGCTCTACAGCTTCTCCTCCGGCGATAACCTGGTCAAGATCCTTGATCGATATGGGAAACAGCATGCTGATGATCTTAAAGCCTTGGATCATCAGTCAAAAGCTCTGAACTATGGTTCACACTATGAGCTACTTGAACTTGTGGATAGCAAGCTTGTGGGATCAAATGTCAAAAATGTGAGTATCGATGCTCTTGTTCAACTGGAGGAACACCTTGAGACTGCCCTCTCCGTGACTAGAGCCAAGAAGACCGAACTCATGTTGAAGCTTGTTGAGAATCTTAAAGAAAAGGAGAAAATGCTGAAAGAAGAGAACCAGGTTTTGGCTAGCCAGATGGAGAATAATCATCATGTGGGAGCAGAAGCTGAGATGGAGATGTCACCTGCTGGACAAATCTCCGACAATCTTCCGGTGACTCTCCCACTACTTAATTAGCCACCTTAAATCGGCGGTTGAAATCAAAATCCAAAACATATATAATTATGAAGAAAAAAAAAATAAGATATGTAATTATTCCGCTGATAAGGGCGAGCGTTTGTATATCTTAATACTCTCTCTTTGGCCAAGAGACTTTGTGTGTGATACTTAAGTAGACGGAACTAAGTCAATACTATCTGTTTTAAGACAAAAGGTTGATGAACTTTGTACCTTATTCGTGTGAGAATTGCATCGAGATCTTGAGTGTATGTGTTCTTCACTTCTGTCAAAAACTTGTGTTTGCTTCACAGTGAAGAAGCCTACGGCTTATTTTGCAACAGGGACGTGGCTCTCTCTCTC
CDS Sequence
Protein Sequence