Gene Details:

• Gene ID: AT4G30870
• Gene Symbol: ATMUS81, MUS81
• Gene Name: ARABIDOPSIS THALIANA MMS AND UV SENSITIVE 81, MMS AND UV SENSITIVE 81
• Description: Restriction endonuclease, type II-like superfamily protein;(source:Araport11)
• TAIR Accession: locus:2126729
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT4G30870.1

Gene Ontology:

• GO:0048476  — part of — Holliday junction resolvase complex
• GO:0006281  — involved in — DNA repair
• GO:0000727  — involved in — double-strand break repair via break-induced replication
• GO:0007129  — acts upstream of or within — homologous chromosome pairing at meiosis
• GO:0006308  — involved in — DNA catabolic process
• GO:0000724  — acts upstream of or within — double-strand break repair via homologous recombination
• GO:0000712  — involved in — resolution of meiotic recombination intermediates
• GO:0005634  — is active in — nucleus
• GO:0051026  — acts upstream of or within — chiasma assembly
• GO:0031573  — involved in — mitotic intra-S DNA damage checkpoint signaling
• GO:0006974  — involved in — DNA damage response
• GO:0000794  — located in — condensed nuclear chromosome
• GO:0000723  — involved in — telomere maintenance
• GO:0008821  — contributes to — crossover junction DNA endonuclease activity
• GO:0006281  — acts upstream of or within — DNA repair
• GO:0048257  — enables — 3-flap endonuclease activity GO:0048257 17230 F catalytic activity IBA none PANTHER:PTN000335543|UniProtKB:Q96NY9 Communication:501741973 2021-04-02 AT4G30870 locus:2126729 AT4G30870 involved in DNA repair GO:0006281 4741 P response to stress IMP none Publication:501767564|PMID:26704385 2021-03-31 AT4G30870 locus:2126729 AT4G30870 involved in resolution of meiotic recombination intermediates GO:0000712 11804 P cell cycle IBA none PANTHER:PTN000335543|SGD:S000002794|WB:WBGene00016602|PomBase:SPCC4G3.05c Communication:501741973 2023-06-07 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within chiasma assembly GO:0051026 18777 P reproduction IGI double mutant analysis Tair:gene:2130912 Publication:501723987|PMID:18182028 TAIR 2008-07-08 AT4G30870 locus:2126729 AT4G30870 involved in mitotic intra-S DNA damage checkpoint signaling GO:0031573 21660 P other cellular processes IBA none PANTHER:PTN000335543|PomBase:SPCC4G3.05c Communication:501741973 2021-03-31 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within chiasma assembly GO:0051026 18777 P cellular component organization IGI double mutant analysis Tair:gene:2130912 Publication:501723987|PMID:18182028 TAIR 2008-07-08 AT4G30870 locus:2126729 AT4G30870 involved in resolution of meiotic recombination intermediates GO:0000712 11804 P cellular component organization IBA none PANTHER:PTN000335543|SGD:S000002794|WB:WBGene00016602|PomBase:SPCC4G3.05c Communication:501741973 2023-06-07 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within homologous chromosome pairing at meiosis GO:0007129 7366 P cell cycle IGI double mutant analysis AGI_LocusCode:AT4G14970 Publication:501778430|PMID:29352063 TAIR 2018-03-22 AT4G30870 locus:2126729 AT4G30870 involved in DNA damage response GO:0006974 4725 P response to stress IEP none Publication:501729770|PMID:17696612 2021-03-31 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within DNA repair GO:0006281 4741 P other cellular processes IMP analysis of physiological response NONE Publication:501720007|PMID:16945961 TAIR 2006-11-13 AT4G30870 locus:2126729 AT4G30870 involved in resolution of meiotic recombination intermediates GO:0000712 11804 P other cellular processes IBA none PANTHER:PTN000335543|SGD:S000002794|WB:WBGene00016602|PomBase:SPCC4G3.05c Communication:501741973 2023-06-07 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within chiasma assembly GO:0051026 18777 P other cellular processes IGI double mutant analysis Tair:gene:2130912 Publication:501723987|PMID:18182028 TAIR 2008-07-08 AT4G30870 locus:2126729 AT4G30870 enables endonuclease activity GO:0004519 2238 F nuclease activity ISS Sequence similarity (homologue of/most closely related to) SGD:MUS81 Publication:501720007|PMID:16945961 TAIR 2006-12-18 AT4G30870 gene:3439322 AT4G30870.1 located in nucleus GO:0005634 537 C nucleus ISM predicted protein features AnalysisReference:501780126 rkaundal 2018-08-31 AT4G30870 locus:2126729 AT4G30870 involved in double-strand break repair via break-induced replication GO:0000727 13296 P response to stress IBA none PANTHER:PTN000335543|SGD:S000002794 Communication:501741973 2021-03-31 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within homologous chromosome pairing at meiosis GO:0007129 7366 P other cellular processes IGI double mutant analysis AGI_LocusCode:AT4G14970 Publication:501778430|PMID:29352063 TAIR 2018-03-22 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within homologous chromosome pairing at meiosis GO:0007129 7366 P cellular component organization IGI double mutant analysis AGI_LocusCode:AT4G14970 Publication:501778430|PMID:29352063 TAIR 2018-03-22 AT4G30870 locus:2126729 AT4G30870 involved in DNA catabolic process GO:0006308 4730 P catabolic process IEA none InterPro:IPR033309 AnalysisReference:501756966 2023-06-11 AT4G30870 locus:2126729 AT4G30870 involved in resolution of meiotic recombination intermediates GO:0000712 11804 P reproduction IBA none PANTHER:PTN000335543|SGD:S000002794|WB:WBGene00016602|PomBase:SPCC4G3.05c Communication:501741973 2023-06-07 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within mitotic recombination GO:0006312 6401 P DNA metabolic process IMP none NONE Publication:501720007|PMID:16945961 TAIR 2006-11-13 AT4G30870 locus:2126729 AT4G30870 involved in DNA catabolic process GO:0006308 4730 P other cellular processes IEA none InterPro:IPR033309 AnalysisReference:501756966 2023-06-11 AT4G30870 locus:2126729 AT4G30870 contributes to crossover junction DNA endonuclease activity GO:0008821 2009 F nuclease activity IBA none PANTHER:PTN000335543|SGD:S000002794 Communication:501741973 2022-11-23 AT4G30870 locus:2126729 AT4G30870 acts upstream of or within double-strand break repair via homologous recombination GO:0000724 14226 P other cellular processes IMP biochemical/chemical analysis NONE Publication:501740121|PMID:20971895 TAIR 2010-12-21 AT4G30870 locus:2126729 AT4G30870 enables 3-flap endonuclease activity

Germplasm Phenotype:

• GABI_113F11  — Deficiency in homologous recombination in somatic cells but only after induction by genotoxic stress.
• SALK_107515  — Deficiency in homologous recombination in somatic cells but only after induction by genotoxic stress.
• fancd2-1 mus81-2  — increased percentage of cells with univalents as compared to single mutant mus81-2, also observed cells with more than one chromosome pair affected in the double mutant.
• hrq1-1 mus81-1  — Significant reduction of FW after treatment with cisplatin and MMC.
• msh4 mus81-2 fancd2-1  — Fewer bivalents then the msh4 mus81-2 double mutant; 67.6% of the msh4 mus81-2 fancd2-1 triple mutant meiocytes contain no bivalents (2.4% in the double mutant); 26.5% of the cells display one, and only 5.9% have two bivalents (compared to 31.7% and 46.3%, respectively, in the msh4 mus81-2 double mutants).
• mus81-1  — Severe reduction in relative FW when exposed to crosslinkers cisplatin and MMC.

Literature:

• Functional hammerhead ribozymes naturally encoded in the genome of Arabidopsis thaliana.   DOI:  10.1105/tpc.105.032730 ;  PMID:  15937227
• The role of AtMUS81 in DNA repair and its genetic interaction with the helicase AtRecQ4A.   DOI:  10.1093/nar/gkl576 ;  PMID:  16945961
• The road to crossovers: plants have their say.   DOI:  10.1016/j.tig.2006.12.007 ;  PMID:  17208327
• Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday junctions.   DOI:  10.1104/pp.109.136846 ;  PMID:  19339504
• RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana.   DOI:  10.1105/tpc.110.078568 ;  PMID:  20971895
• Systematic identification of functional plant modules through the integration of complementary data sources.   DOI:  10.1104/pp.112.196725 ;  PMID:  22589469
• MHF1 plays Fanconi anaemia complementation group M protein (FANCM)-dependent and FANCM-independent roles in DNA repair and homologous recombination in plants.   DOI:  10.1111/tpj.12507 ;  PMID:  24635147
• The Arabidopsis thaliana homolog of the helicase RTEL1 plays multiple roles in preserving genome stability.   DOI:  10.1105/tpc.114.132472 ;  PMID:  25516598
• The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81.   DOI:  10.1093/nar/gkv208 ;  PMID:  25779053
• Formation of interference-sensitive meiotic cross-overs requires sufficient DNA leading-strand elongation.   DOI:  10.1073/pnas.1507165112 ;  PMID:  26392549
• The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.   DOI:  10.1104/pp.15.00806 ;  PMID:  26474640
• The Structure-Specific Endonucleases MUS81 and SEND1 Are Essential for Telomere Stability in Arabidopsis.   DOI:  10.1105/tpc.15.00898 ;  PMID:  26704385
• Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant.   DOI:  10.1371/journal.pgen.1005301 ;  PMID:  26147458
• The DNA translocase RAD5A acts independently of the other main DNA repair pathways, and requires both its ATPase and RING domain for activity in Arabidopsis thaliana.   DOI:  10.1111/tpj.13602 ;  PMID:  28509359
• Arabidopsis thaliana FANCD2 Promotes Meiotic Crossover Formation.   DOI:  10.1105/tpc.17.00745 ;  PMID:  29352063
• The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A.   DOI:  10.1111/nph.15109 ;  PMID:  29577315
• The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates.   DOI:  10.1371/journal.pgen.1007674 ;  PMID:  30222730
• The Protease WSS1A, the Endonuclease MUS81, and the Phosphodiesterase TDP1 Are Involved in Independent Pathways of DNA-protein Crosslink Repair in Plants.   DOI:  10.1105/tpc.18.00824 ;  PMID:  30760561
• An Arabidopsis FANCJ helicase homologue is required for DNA crosslink repair and rDNA repeat stability.   DOI:  10.1371/journal.pgen.1008174 ;  PMID:  31120885
• ATM controls meiotic DNA double-strand break formation and recombination and affects synaptonemal complex organization in plants.   DOI:  10.1093/plcell/koab045 ;  PMID:  33659989
• Comparative transcriptomic analysis of thermally stressed Arabidopsis thaliana meiotic recombination mutants.   DOI:  10.1186/s12864-021-07497-2 ;  PMID:  33711924
• The repair of topoisomerase 2 cleavage complexes in Arabidopsis.   DOI:  10.1093/plcell/koab228 ;  PMID:  34524446
• Regulation of interference-sensitive crossover distribution ensures crossover assurance in Arabidopsis.   DOI:  10.1073/pnas.2107543118 ;  PMID:  34795056
• The FANCC-FANCE-FANCF complex is evolutionarily conserved and regulates meiotic recombination.   DOI:  10.1093/nar/gkac1244 ;  PMID:  36652992
• SMC5/6 complex-mediated SUMOylation stimulates DNA-protein cross-link repair in Arabidopsis.   DOI:  10.1093/plcell/koad020 ;  PMID:  36705512
• The Arabidopsis Hop1 homolog ASY1 mediates cross-over assurance and interference.   DOI:  10.1093/pnasnexus/pgac302 ;  PMID:  36992817
• Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday junctions.   DOI:  10.1104/pp.109.136846 ;  PMID:  19339504

Sequences: