Gene Details:

• Gene ID: AT4G29840
• Gene Symbol: MKO1, MTO2, TS, TSY1
• Gene Name: MOOTS KOOM 1, METHIONINE OVER-ACCUMULATOR 2, THREONINE SYNTHASE, THREONINE SYNTHASE 1
• Description: Pyridoxal-5-phosphate-dependent enzyme family protein;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT4G29840.1

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
• PO:0000293  — guard cell — célula guardiana (Spanish, exact), occlusive cell (exact), 孔辺細胞 (Japanese, exact)

Germplasm Phenotype:

• CS208  — methionine over-accumulation; in young rosettes: soluble Met over-accumulates 20-fold greater than Ws wild-type; S-adenosylmethionine (SAM) over-accumulates 3-fold; and soluble Thr levels are 6% of that in wild-type; line is also resistant to 10uM ethionine, a toxic analogue of Met; 1:2:1 segregation of resistance to 10uM L-ethionine phenotype
• CS73272  — Seedlings develop a short primary root, 2 to 4 mm, as a result of root apical meristem (RAM) exhaustion. At five days after germination (dag), the RAM exhaustion starts and by 10-12 dag no RAM is present. Therefore, the mutant shows determinate root growth in the primary root and most of the lateral roots. Shoot growth is much slower and shoots are shorter than in Wt Ler, but the mutant produces a functional inflorescence and viable seeds.
• mto2-2  — The phenotype is also described in Hernández-Barrera et al., 2011, DOI 10.1007/s00425-011-1470-4. At that time the mutant name was moots koom1, mko1 (short root in Mayan). It was renamed to mto2-2 when a gene responsible for mutation was identified. Seedlings develop a short primary root, 2 to 4 mm. The arrested growth is a result of the root apical meristem (RAM) exhaustion. In just germinated seedlings, the RAM organization and tissue patterning are not affected. However, at five days after germination (dag), the RAM exhaustion starts and by 10-12 dag no RAM is present. Therefore, the mutant shows typical determinate root growth. Most of lateral roots also show determinate growth. Shoot growth is much slower and shoots are shorter than in Wt, but the mutant produces functional inflorescence and viable seeds.

Function-related keywords:

• shoot axis apex ,  leaf lamina base ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen ,  guard cell

Literature:

• Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana.   DOI:  10.1111/j.1365-313X.2004.02329.x ;  PMID:  15703056
• Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.   DOI:  10.1104/pp.108.121038 ;  PMID:  18650403
• Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.   DOI:  10.1104/pp.108.126375 ;  PMID:  18775970
• Species specific amino acid sequence-protein local structure relationships: An analysis in the light of a structural alphabet.   DOI:  10.1016/j.jtbi.2011.01.047 ;  PMID:  21333657
• Aspartate-Derived Amino Acid Biosynthesis in Arabidopsis thaliana.   DOI:  10.1199/tab.0121 ;  PMID:  22303247
• Capturing the phosphorylation and protein interaction landscape of the plant TOR kinase.   DOI:  10.1038/s41477-019-0378-z ;  PMID:  30833711
• A mutation in THREONINE SYNTHASE 1 uncouples proliferation and transition domains of the root apical meristem: experimental evidence and in silico proposed mechanism.   DOI:  10.1242/dev.200899 ;  PMID:  36278862
• Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.   DOI:  10.1371/journal.pone.0001994 ;  PMID:  18431481
• Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling.   DOI:  10.1016/j.jprot.2017.06.004 ;  PMID:  28627464

Sequences: