Gene Details:
- Gene ID: AT4G27330
- Gene Symbol: NZZ, SPL
- Gene Name: NOZZLE, SPOROCYTELESS
- Description: sporocyteless (SPL);(source:Araport11)
- TAIR Accession: locus:2131729
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0009009 — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
- PO:0009031 — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
- PO:0009046 — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
- PO:0025022 — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
Gene Ontology:
- GO:0005515 — enables — protein binding
- GO:0048653 — acts upstream of or within — anther development
- GO:0003700 — enables — DNA-binding transcription factor activity
- GO:0005634 — located in — nucleus
- GO:0048533 — acts upstream of or within — sporocyte differentiation
Germplasm Phenotype:
- CS6586 — Defective in sporocyte formation (resulting in the absence of microspores and megaspores) and anther wall formation; hypodermal cells of anthers at stage 2 enlarge and differentiate into archesporial cells normally; archesporial cells undergo periclinal divisions to form the primary parietal cell (PPC) layer and the primary sporogenous cell (PSC) layer at stage 3 anthers; PSCs do not form microsporocytes, becoming vacuolated at stage 4 anthers; parietal cells are also affected, because the PPCs go through only one division to give rise to two SPC layers that fail to divide further and differentiate; there is no endothecium or tapetum formation; anthers are composed of highly vacuolated parenchyma cells at anthesis; archesporial cells fail to elongate and differentiate into megasporocytes; nucellar development but not integument development is affected. Delayed plant senescence; sterile plants (complete male and female sterility); flowers have normal external morphology and the same number of organs as wild type, anthers are white with no pollen grains at anthesis during stage 13-14; carpels look morphologically normal but are infertile when pollinated with wild-type pollen.
- spl — Absence of megaspores and microspores in homozygous mutant plants
Function-related keywords:
Literature:
- NOZZLE regulates proximal-distal pattern formation, cell proliferation and early sporogenesis during ovule development in Arabidopsis thaliana. DOI: 10.1242/dev.127.19.4227 ; PMID: 10976054
- The SPOROCYTELESS gene of Arabidopsis is required for initiation of sporogenesis and encodes a novel nuclear protein. DOI: 10.1101/gad.13.16.2108 ; PMID: 10465788
- NOZZLE links proximal-distal and adaxial-abaxial pattern formation during ovule development in Arabidopsis thaliana. DOI: 10.1242/dev.129.18.4291 ; PMID: 12183381
- The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESS. DOI: 10.1038/nature02733 ; PMID: 15254538
- Pattern formation during early ovule development in Arabidopsis thaliana. DOI: 10.1016/j.ydbio.2004.05.037 ; PMID: 15328016
- Organ polarity in Arabidopsis. NOZZLE physically interacts with members of the YABBY family. DOI: 10.1104/pp.104.040154 ; PMID: 15299139
- Analysis of the female gametophyte transcriptome of Arabidopsis by comparative expression profiling. DOI: 10.1104/pp.105.067314 ; PMID: 16299181
- Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM1 (DYT1) encoding a putative bHLH transcription factor. DOI: 10.1242/dev.02463 ; PMID: 16831835
- Differential gene expression in Arabidopsis wild-type and mutant anthers: insights into anther cell differentiation and regulatory networks. DOI: 10.1111/j.1365-313X.2007.03217.x ; PMID: 17666023
- Global expression profiling applied to the analysis of Arabidopsis stamen development. DOI: 10.1104/pp.107.104422 ; PMID: 17905860
- ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development. DOI: 10.1111/j.1365-313X.2007.03375.x ; PMID: 18036205
- The SPOROCYTELESS/NOZZLE gene is involved in controlling stamen identity in Arabidopsis. DOI: 10.1104/pp.109.145896 ; PMID: 19726570
- Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development. DOI: 10.1073/pnas.0912333107 ; PMID: 20231470
- Arabidopsis basic leucine-zipper transcription factors TGA9 and TGA10 interact with floral glutaredoxins ROXY1 and ROXY2 and are redundantly required for anther development. DOI: 10.1104/pp.110.159111 ; PMID: 20805327
- miR156-targeted and nontargeted SBP-box transcription factors act in concert to secure male fertility in Arabidopsis. DOI: 10.1105/tpc.110.079343 ; PMID: 21177480
- AMS-dependent and independent regulation of anther transcriptome and comparison with those affected by other Arabidopsis anther genes. DOI: 10.1186/1471-2229-12-23 ; PMID: 22336428
- The Arabidopsis LFR gene is required for the formation of anther cell layers and normal expression of key regulatory genes. DOI: 10.1093/mp/sss024 ; PMID: 22461668
- The transcription factors BEL1 and SPL are required for cytokinin and auxin signaling during ovule development in Arabidopsis. DOI: 10.1105/tpc.112.100164 ; PMID: 22786869
- A survey of dominant mutations in Arabidopsis thaliana. DOI: 10.1016/j.tplants.2012.08.006 ; PMID: 22995285
- Interaction between two timing microRNAs controls trichome distribution in Arabidopsis. DOI: 10.1371/journal.pgen.1004266 ; PMID: 24699192
- Comparative expression profiling reveals gene functions in female meiosis and gametophyte development in Arabidopsis. DOI: 10.1111/tpj.12657 ; PMID: 25182975
- The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis ovule development. DOI: 10.1038/cr.2014.145 ; PMID: 25378179
- SPOROCYTELESS is a novel embryophyte-specific transcription repressor that interacts with TPL and TCP proteins in Arabidopsis. DOI: 10.1016/j.jgg.2014.08.009 ; PMID: 25527103
- Deciphering the Role of POLYCOMB REPRESSIVE COMPLEX1 Variants in Regulating the Acquisition of Flowering Competence in Arabidopsis. DOI: 10.1104/pp.15.00073 ; PMID: 25897002
- Phosphorylation of SPOROCYTELESS/NOZZLE by the MPK3/6 Kinase Is Required for Anther Development. DOI: 10.1104/pp.16.01765 ; PMID: 28209842
- C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. DOI: 10.3389/fpls.2017.01689 ; PMID: 29021807
- Arabidopsis PIRL6 Is Essential for Male and Female Gametogenesis and Is Regulated by Alternative Splicing. DOI: 10.1104/pp.18.00329 ; PMID: 30206104
- Role for the shoot apical meristem in the specification of juvenile leaf identity in Arabidopsis. DOI: 10.1073/pnas.1817853116 ; PMID: 31023887
- Establishing a framework for female germline initiation in the plant ovule. DOI: 10.1093/jxb/erz212 ; PMID: 31063548
- MicroRNA166 Monitors SPOROCYTELESS/NOZZLE for Building of the Anther Internal Boundary. DOI: 10.1104/pp.19.00336 ; PMID: 31248965
- BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis. DOI: 10.1016/j.molp.2019.06.006 ; PMID: 31229643
- The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana. DOI: 10.3390/cells10040904 ; PMID: 33920019
- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition. DOI: 10.1016/j.celrep.2021.109348 ; PMID: 34260932
- ATBS1-INTERACTING FACTOR 2 Negatively Modulates Pollen Production and Seed Formation in Arabidopsis. DOI: 10.3389/fpls.2021.704958 ; PMID: 34386030
- Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis. DOI: 10.1038/s41467-022-34723-6 ; PMID: 36379956
- Reproductive competence is regulated independently of vegetative phase change in Arabidopsis thaliana. DOI: 10.1016/j.cub.2022.12.029 ; PMID: 36634678
Sequences:
cDNA Sequence
- >AT4G27330.1
GTATATGAACACTAAAGAGATACACACACACAAAAAGAGAGCAGAAACAAAACACACACACTTAAAGCTTTCGTCTTTACCTCTTCCCTTCTCTCTCTCTATCTAAAAAGAGTTCCGAGAAGAAGATCATCATCAATGGCGACTTCTCTCTTCTTCATGTCAACAGATCAAAACTCCGTCGGAAACCCAAACGATCTTCTGAGAAACACCCGTCTTGTCGTCAACAGCTCCGGCGAGATCCGGACAGAGACACTGAAGAGTCGTGGTCGGAAACCAGGATCGAAGACAGGTCAGCAAAAACAGAAGAAACCAACGTTGAGAGGAATGGGTGTAGCAAAGCTCGAGCGTCAGAGAATCGAAGAAGAAAAGAAGCAACTCGCCGCCGCCACAGTCGGAGACACGTCATCAGTAGCATCGATCTCTAACAACGCTACCCGTTTACCCGTACCGGTAGACCCGGGTGTTGTGCTACAAGGCTTCCCAAGCTCACTCGGGAGCAACAGGATCTATTGTGGTGGAGTCGGGTCGGGTCAGGTTATGATCGACCCGGTTATTTCTCCATGGGGTTTTGTTGAGACCTCCTCCACTACTCATGAGCTCTCTTCAATCTCAAATCCTCAAATGTTTAACGCTTCTTCCAATAATCGCTGTGACACTTGCTTCAAGAAGAAACGTTTGGATGGTGATCAGAATAATGTAGTTCGATCCAACGGTGGTGGATTTTCGAAATACACAATGATTCCTCCTCCGATGAACGGCTACGATCAGTATCTTCTTCAATCAGATCATCATCAGAGGAGCCAAGGTTTCCTTTATGATCATAGAATCGCTAGAGCAGCTTCAGTTTCTGCTTCTAGTACTACTATTAATCCTTATTTCAACGAGGCAACAAATCATACGGGACCAATGGAGGAATTTGGGAGCTACATGGAAGGAAACCCTAGAAATGGATCAGGAGGTGTGAAGGAGTACGAGTTTTTTCCGGGGAAATATGGTGAAAGAGTTTCAGTGGTGGCTAAAACGTCGTCACTCGTAGGTGATTGCAGTCCTAATACCATTGATTTGTCCTTGAAGCTTTAAATGTTTTATCTTTCTATATTGATTTAAACAAAATCGTCTCTTTAAAGAAAAAACATTTTAAGTAGATGAAAGTAAGAAACAGAAGAAAAAAAAGAGAGAGCCTTTTTTGGTGTATGCATCTGAGAGCTGAGTCGAAAGAAAGATTCAGCTTTTGGATTACCCTTTTGGTTGTTTATTATGAGATTCTAACCTAAACACTCAGACATATATGTTCTGTTCTCTTCCTTAATTGTTGTCATGAAACTTCTC
CDS Sequence
Protein Sequence