Gene Details:
- Gene ID: AT3G48100
- Gene Symbol: ARR5, ATRR2, IBC6, RR5
- Gene Name: response regulator 5, ARABIDOPSIS THALIANA RESPONSE REGULATOR 2, INDUCED BY CYTOKININ 6, response regulator 5
- Description: response regulator 5;(source:Araport11)
- TAIR Accession: locus:2097870
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0000146 — abscission zone — zona de absición (Spanish, exact), 離層 (Japanese, exact), abscission layer (related)
- PO:0006085 — root meristem — meristema de la raíz (Spanish, exact), 根分裂組織 (Japanese, exact), meristematic zone (related)
- PO:0020144 — apical meristem — AM (exact), meristema apical (Spanish, exact), 頂端分裂組織 (Japanese, exact), primary meristem (related), promeristem (broad)
- PO:0003011 — root vascular system — sistema vascular de la raíz (Spanish, exact), 根維管系 (Japanese, exact), root vein (related)
- PO:0020148 — shoot apical meristem — SAM (exact), meristema apical del epiblasto (epiblastema) (Spanish, exact), 茎頂分裂組織 (Japanese, exact), primary shoot meristem (related), promeristem (broad)
- PO:0000037 — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
- PO:0008019 — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0009006 — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
- PO:0009009 — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
- PO:0009025 — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
- PO:0009031 — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
- PO:0009046 — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
- PO:0009052 — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
- PO:0020030 — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
- PO:0020038 — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
- PO:0020100 — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
- PO:0020137 — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
- PO:0025022 — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
- PO:0000026 — primary root tip — punta de la raíz primaria (Spanish, exact), 一次根端 (Japanese, exact)
- PO:0003021 — central root cap of primary root — caliptra de la raíz central de la raíz primaria (Spanish, exact), 一次根の中心根冠 (Japanese, exact)
- PO:0003022 — lateral root cap of primary root — caliptra de la raíz lateral de la raíz primaria (Spanish, exact), 一次根の側根冠 (Japanese, exact)
- PO:0006307 — root procambium — procambium de la raíz (Spanish, exact), 根前形成層 (Japanese, exact)
Gene Ontology:
- GO:0005515 — enables — protein binding
- GO:0009736 — acts upstream of or within — cytokinin-activated signaling pathway
- GO:0005737 — located in — cytoplasm
- GO:0009735 — acts upstream of or within — response to cytokinin
- GO:0006355 — acts upstream of or within — regulation of DNA-templated transcription
- GO:0010114 — acts upstream of or within — response to red light
- GO:0000156 — enables — phosphorelay response regulator activity
- GO:0007623 — acts upstream of or within — circadian rhythm
- GO:0005634 — located in — nucleus
Germplasm Phenotype:
- CS25267 — Reduced rosette size when grown under short-day conditions; cytokinin sensitivity similar to wild type in seedling root elongation and seedling lateral root formation; increased sensitivity to red light.
- CS25272 — No adult visible phenotype; the reduced rosette size of arr5 is not enhanced but suppressed by the arr6 mutation (suggesting antagonistic function); subtle difference in cytokinin sensitivity of root elongation compared to the wild type; intermediate cytokinin sensitivity between the wild type and arr3,4,5,6, arr5,6,8,9 and arr3,4,8,9 quadruple mutants in seedling lateral root formation; somewhat increased sensitivity to red light.
- CS25273 — Adult phenotype similar to the arr5 parent: reduced rosette size when grown under short-day conditions; increased cytokinin inhibition of root elongation but intermediate between arr3,4,5,6 and the wild type; intermediate cytokinin sensitivity between the wild type and arr3,4,5,6, arr5,6,8,9 and arr3,4,8,9 quadruple mutants in seedling lateral root formation; increased sensitivity to red light.
- CS25276 — The elongated petioles of arr4 and arr3,4 are enhanced, but the overall rosette size is similar to that of the wild-type parent; the increased petiole elongation suggests that although ARR5 and ARR6 may act antagonistically to each other in regulating rosette size, as a pair they still function additively with ARR3 and ARR4 in the regulation of petiole elongation; root elongation responses intermediate between arr3,4,5,6,8,9 and the wild type, but significantly more sensitive than arr3,4; intermediate cytokinin sensitivity in seedling lateral root formation, between the wild type and the arr3,4,5,6,8,9 hextuple mutant, with the sensitivity of arr3,4,8,9 closest to arr3,4,5,6,8,9; increased sensitivity to red light.
- CS25277 — No adult visible phenotype; root elongation responses intermediate between arr3,4,5,6,8,9 and the wild type; but significantly more sensitive than arr8,9; intermediate cytokinin sensitivity in seedling lateral root formation, between the wild type and the arr3,4,5,6,8,9 hextuple mutant, with the sensitivity of arr3,4,8,9 closest to arr3,4,5,6,8,9; intermediate cytokinin sensitivity in seedling lateral root formation, between the wild type and the arr3,4,5,6,8,9 hextuple mutant, with the sensitivity of arr3,4,8,9 closest to arr3,4,5,6,8,9.
- CS25279 — Intermediate petiole length between arr3,4,5,6 and the wild type (suggesting complex interactions between these genes); roots are shorter and have significantly fewer lateral roots than the wild type in the absence of exogenous cytokinin; increased cytokinin hypersensitivity in seedling root elongation, seedling lateral root formation, hypocotyls/ shoot initiation and leaf senescence delay; decreased sensitivity to red light compared to wild type.
Function-related keywords:
- abscission zone , root meristem , apical meristem , root vascular system , shoot apical meristem , shoot axis apex , leaf lamina base , root , shoot system , plant embryo , vascular leaf , sepal , flower , inflorescence flower pedicel , cotyledon , petiole , hypocotyl , leaf apex , collective leaf structure , pollen , primary root tip , central root cap of primary root , lateral root cap of primary root , root procambium
Literature:
- Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis. DOI: 10.1105/tpc.10.6.1009 ; PMID: 9634588
- Stress-responsive expression of genes for two-component response regulator-like proteins in Arabidopsis thaliana. DOI: 10.1016/s0014-5793(98)00418-9 ; PMID: 9607306
- Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. DOI: 10.1104/pp.124.4.1706 ; PMID: 11115887
- Two-component circuitry in Arabidopsis cytokinin signal transduction. DOI: 10.1038/35096500 ; PMID: 11574878
- Two-component signal transduction pathways in Arabidopsis. DOI: 10.1104/pp.005504 ; PMID: 12068096
- Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. DOI: 10.1105/tpc.014928 ; PMID: 14555694
- Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. DOI: 10.1105/tpc.018978 ; PMID: 14973166
- Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants. DOI: 10.1104/pp.104.040113 ; PMID: 15194821
- Two cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, differ in their ligand specificity in a bacterial assay. DOI: 10.1093/pcp/pch132 ; PMID: 15509853
- Characterization of an Arabidopsis gene that mediates cytokinin signaling in shoot apical meristem development. DOI: S1016-8478(23)13178-5 ; PMID: 15995350
- Arabidopsis KNOXI proteins activate cytokinin biosynthesis. DOI: 10.1016/j.cub.2005.07.060 ; PMID: 16139212
- Activation of genes presumed in cytokinin signal pathway of two-component system in response to the increased cytokinin contents in ipt-GUS transgenic Arabidopsis. DOI: NA ; PMID: 16361787
- Arabidopsis response regulators ARR3 and ARR4 play cytokinin-independent roles in the control of circadian period. DOI: 10.1105/tpc.105.037994 ; PMID: 16326927
- WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. DOI: 10.1038/nature04270 ; PMID: 16372013
- Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. DOI: 10.1073/pnas.0505150103 ; PMID: 16407152
- Developmental steps in acquiring competence for shoot development in Arabidopsis tissue culture. DOI: 10.1007/s00425-007-0565-4 ; PMID: 17581762
- Cytokinin receptors are involved in alkamide regulation of root and shoot development in Arabidopsis. DOI: 10.1104/pp.107.107953 ; PMID: 17965178
- Cytokinin regulates type-A Arabidopsis Response Regulator activity and protein stability via two-component phosphorelay. DOI: 10.1105/tpc.107.052662 ; PMID: 18065689
- Toward an interaction map of the two-component signaling pathway of Arabidopsis thaliana. DOI: 10.1021/pr0703831 ; PMID: 18642946
- Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development. DOI: 10.1105/tpc.108.059584 ; PMID: 18723577
- Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. DOI: 10.1111/j.1365-313X.2008.03715.x ; PMID: 18980654
- The purine derivative PI-55 blocks cytokinin action via receptor inhibition. DOI: 10.1111/j.1742-4658.2008.06777.x ; PMID: 19032596
- Genome-wide comparative analysis of type-A Arabidopsis response regulator genes by overexpression studies reveals their diverse roles and regulatory mechanisms in cytokinin signaling. DOI: 10.1038/cr.2009.88 ; PMID: 19621034
- The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. DOI: 10.1111/j.1365-313X.2009.03992.x ; PMID: 19682286
- Acquiring competence for shoot development in Arabidopsis: ARR2 directly targets A-type ARR genes that are differentially activated by CIM preincubation. DOI: 10.4161/psb.3.2.4958 ; PMID: 19704721
- RNAi suppression of RPN12a decreases the expression of type-A ARRs, negative regulators of cytokinin signaling pathway, in Arabidopsis. DOI: 10.1007/s10059-009-0132-x ; PMID: 19812900
- Cytokinin receptor antagonists derived from 6-benzylaminopurine. DOI: 10.1016/j.phytochem.2010.01.018 ; PMID: 20189204
- CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling. DOI: 10.1093/pcp/pcq129 ; PMID: 20802224
- The specificity of cytokinin signalling in Arabidopsis thaliana is mediated by differing ligand affinities and expression profiles of the receptors. DOI: 10.1111/j.1365-313X.2011.04584.x ; PMID: 21426428
- Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system. DOI: 10.1016/j.phytochem.2011.02.029 ; PMID: 21477822
- Cytokinin antagonizes ABA suppression to seed germination of Arabidopsis by downregulating ABI5 expression. DOI: 10.1111/j.1365-313X.2011.04683.x ; PMID: 21699589
- Narciclasine inhibits the responses of Arabidopsis roots to auxin. DOI: 10.1007/s00425-012-1632-z ; PMID: 22476291
- Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. DOI: 10.1105/tpc.112.098640 ; PMID: 22706288
- Cytokinin receptor-dependent and receptor-independent pathways in the dehydration response of Arabidopsis thaliana. DOI: 10.1016/j.jplph.2012.05.007 ; PMID: 22704545
- AXR1 promotes the Arabidopsis cytokinin response by facilitating ARR5 proteolysis. DOI: 10.1111/tpj.12098 ; PMID: 23279608
- SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. DOI: 10.1104/pp.15.01112 ; PMID: 26304848
- Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. DOI: 10.1016/j.phytochem.2018.02.015 ; PMID: 29524794
- The Antagonistic Action of Abscisic Acid and Cytokinin Signaling Mediates Drought Stress Response in Arabidopsis. DOI: 10.1016/j.molp.2018.05.001 ; PMID: 29753021
- The bacterial volatile dimethyl-hexa-decylamine reveals an antagonistic interaction between jasmonic acid and cytokinin in controlling primary root growth of Arabidopsis seedlings. DOI: 10.1007/s00709-018-1327-9 ; PMID: 30382422
- ARR22 overexpression can suppress plant Two-Component Regulatory Systems. DOI: 10.1371/journal.pone.0212056 ; PMID: 30742656
- A fluorescence-based high-throughput screening method for cytokinin translocation mutants. DOI: 10.1186/s13007-020-00676-4 ; PMID: 33042209
- Diversified Regulation of Cytokinin Levels and Signaling During Botrytis cinerea Infection in Arabidopsis. DOI: 10.3389/fpls.2021.584042 ; PMID: 33643340
- Azospirillum brasilense Sp245 triggers cytokinin signaling in root tips and improves biomass accumulation in Arabidopsis through canonical cytokinin receptors. DOI: 10.1007/s12298-021-01036-9 ; PMID: 34539107
- Arabidopsis ATXR2 represses de novo shoot organogenesis in the transition from callus to shoot formation. DOI: 10.1016/j.celrep.2021.109980 ; PMID: 34758306
- Crosstalk of Cytokinin with Ethylene and Auxin for Cell Elongation Inhibition and Boron Transport in Arabidopsis Primary Root under Boron Deficiency. DOI: 10.3390/plants11182344 ; PMID: 36145745
- Two-component circuitry in Arabidopsis cytokinin signal transduction. DOI: 10.1038/35096500 ; PMID: 11574878
- Two-component signal transduction pathways in Arabidopsis. DOI: 10.1104/pp.005504 ; PMID: 12068096
- Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots. DOI: 10.1073/pnas.0510607103 ; PMID: 16581911
Sequences:
cDNA Sequence
- >AT3G48100.1
AATTAGCCACGCGCTATCAGACAGACAAAATCCCACAGATATGCAAAGATCTCTCAGAATCCTCTCCCCATATCATATTTTTCTCTTTTCCCTCTCCTTCTTTCTTCCTTTATAAATCCATTTATTCTCCTCTCATCTCTCAGCAAAATCAAATCCTCATAGTTGATTCTCTCTATCTCTCTCACGAGTCACGATCCTACTCTTCTTGATATGGCTGAGGTTTTGCGTCCCGAGATGTTAGATATCTCTAACGACACTTCTTCATTAGCATCACCGAAACTTCTTCATGTTCTTGCTGTTGATGATAGTATGGTTGATCGGAAGTTCATCGAGCGGTTACTCAGAGTCTCATCTTGTAAAGTTACTGTTGTCGATAGTGCGACAAGAGCTTTACAATATCTTGGATTAGATGGAGAGAATAATAGTTCGGTTGGATTTGAGGATCTGAAGATTAATTTGATAATGACGGATTACTCTATGCCTGGGATGACTGGATATGAACTACTGAAGAAGATCAAAGAATCATCAGCTTTCAGAGAAATACCAGTTGTGATTATGTCTTCAGAGAACATCTTGCCTCGTATCGATAGATGTCTTGAAGAAGGAGCTGAAGATTTCTTATTGAAGCCTGTGAAATTAGCTGATGTGAAGAGATTAAGAGATTCTTTAATGAAAGCTGAGGAAAGAGCTTTTAAGAATATTATGCACAAGAGAGAGCTTGAAGCTAATGATATCTACTCGCAGCTAAAACGCGCAAAGATCTGAGTTTATCAGATTCCACGTACGATATGTTCTTTAAAAGCTCAAAGATTCACACACATGCATGTCCTGATTCTTTCGGCTTACAATTTTTGAGACATTACGGCTTCTTTTGCTGATAGAACCAAGACTGATCAAGAGTGACAAAGATGGGTTTTTATAAGATAGAAATGTAAATTGTAGACTTGGATTCATTAGAGAGGAGAAGAGATACATTTTTTTTTTCTTTACATGAGAGATATGTAAAATGGTGGTGTGTTATTTTGGAAGTTAACATATGAATTTCTTTTGTTAAGATTACTTAAATAATGATTTAGGAATGGATTAGTAGTTTAGTACCGTTCATTACACGAAGACAAGCTAATCTTTTGTCTAATTAAAATAAAGCTGTCTACATGAATCGAATCCAGCGAGATAAGTTAGTTATTTTAAGCGTTGGTTGGAACACTTTACATGGCTTTTCGTAGAAATTTCCCCAAGTTGGGTTTTATTAAGACCAAGAAAATTCTAGTCTCCTAGACTTTTTTTTGTTTCTTTTGTTAATTTAGTCTCCTAGGCTTTTTCATTCTATCTGAAAGCAAAATATGGTTATGCAATTTGGTTTAGCCAAAAAAACAAACTAAATTTTTCTAGTCCGGTTCATGTAACTTCATAATCAAACAAGTAAAAGTCGAATGTGACGCGTCTTGCC
CDS Sequence
- >AT3G48100.1
ATGGCTGAGGTTTTGCGTCCCGAGATGTTAGATATCTCTAACGACACTTCTTCATTAGCATCACCGAAACTTCTTCATGTTCTTGCTGTTGATGATAGTATGGTTGATCGGAAGTTCATCGAGCGGTTACTCAGAGTCTCATCTTGTAAAGTTACTGTTGTCGATAGTGCGACAAGAGCTTTACAATATCTTGGATTAGATGGAGAGAATAATAGTTCGGTTGGATTTGAGGATCTGAAGATTAATTTGATAATGACGGATTACTCTATGCCTGGGATGACTGGATATGAACTACTGAAGAAGATCAAAGAATCATCAGCTTTCAGAGAAATACCAGTTGTGATTATGTCTTCAGAGAACATCTTGCCTCGTATCGATAGATGTCTTGAAGAAGGAGCTGAAGATTTCTTATTGAAGCCTGTGAAATTAGCTGATGTGAAGAGATTAAGAGATTCTTTAATGAAAGCTGAGGAAAGAGCTTTTAAGAATATTATGCACAAGAGAGAGCTTGAAGCTAATGATATCTACTCGCAGCTAAAACGCGCAAAGATCTGA
Protein Sequence
- >AT3G48100.1
MAEVLRPEMLDISNDTSSLASPKLLHVLAVDDSMVDRKFIERLLRVSSCKVTVVDSATRALQYLGLDGENNSSVGFEDLKINLIMTDYSMPGMTGYELLKKIKESSAFREIPVVIMSSENILPRIDRCLEEGAEDFLLKPVKLADVKRLRDSLMKAEERAFKNIMHKRELEANDIYSQLKRAKI