Gene Details:
- Gene ID: AT3G29350
- Gene Symbol: AHP2
- Gene Name: histidine-containing phosphotransmitter 2
- Description: histidine-containing phosphotransmitter 2;(source:Araport11)
- TAIR Accession: locus:2093817
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0000003 — whole plant — planta entera (Spanish, exact), 植物体全体 (Japanese, exact), clonal colony (related), colony (related), bush (narrow), frutex (narrow), frutices (narrow), gametophyte (narrow), herb (narrow), liana (narrow), prothalli (narrow), prothallium (narrow), prothallus (narrow), seedling (narrow), shrub (narrow), sporophyte (narrow), suffrutex (narrow), suffrutices (narrow), tree (narrow), vine (narrow), woody clump (narrow), genet (broad), ramet (broad)
- PO:0000037 — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
- PO:0008019 — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0009006 — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
- PO:0009009 — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
- PO:0009025 — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
- PO:0009029 — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
- PO:0009031 — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
- PO:0009046 — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
- PO:0009052 — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
- PO:0020030 — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
- PO:0020038 — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
- PO:0020100 — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
- PO:0020137 — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
- PO:0025022 — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
Gene Ontology:
- GO:0009736 — involved in — cytokinin-activated signaling pathway
- GO:0009736 — acts upstream of or within — cytokinin-activated signaling pathway
- GO:0080036 — acts upstream of or within — regulation of cytokinin-activated signaling pathway
- GO:0005515 — enables — protein binding
- GO:0005634 — located in — nucleus
- GO:0009927 — enables — histidine phosphotransfer kinase activity
- GO:0043424 — enables — protein histidine kinase binding
- GO:0005737 — located in — cytoplasm
- GO:0005634 — is active in — nucleus
- GO:0000160 — acts upstream of or within — phosphorelay signal transduction system
- GO:0016310 — involved in — phosphorylation
- GO:0009560 — acts upstream of or within — embryo sac egg cell differentiation
- GO:0005737 — is active in — cytoplasm
- GO:0009557 — acts upstream of or within — antipodal cell differentiation
- GO:0016310 — acts upstream of or within — phosphorylation
- GO:0007165 — acts upstream of or within — signal transduction
- GO:0000160 — involved in — phosphorelay signal transduction system
- GO:0005829 — located in — cytosol
Germplasm Phenotype:
- CS860144 — Inhibition of root elongation is similar to the wild type plants in the presence of various levels of exogenous cytokinin; root elongation in seedlings is inhibited by increasing concentrations of the cytokinin benzyl adenine (BA) in the growth media, showing a sharp decrease in root elongation between 10 and 50 nM BA.
- CS860150 — Inhibition of root elongation is similar to the wild type plants in the presence of various levels of exogenous cytokinin; root elongation in seedlings is inhibited by increasing concentrations of the cytokinin benzyl adenine (BA) in the growth media, showing a sharp decrease in root elongation between 10 and 50 nM BA.
- CS860151 — Inhibition of root elongation is slightly less sensitive than the wild type plants in the presence of 25 nM of cytokinin benzyl adenine (BA) in the growth media.
- CS860152 — Inhibition of root elongation is similar to the wild type plants in the presence of various levels of exogenous cytokinin; root elongation in seedlings is inhibited by increasing concentrations of the cytokinin benzyl adenine (BA) in the growth media, showing a sharp decrease in root elongation between 10 and 50 nM BA.
- CS860153 — Inhibition of root elongation is similar to the wild type plants in the presence of various levels of exogenous cytokinin; root elongation in seedlings is inhibited by increasing concentrations of the cytokinin benzyl adenine (BA) in the growth media, showing a sharp decrease in root elongation between 10 and 50 nM BA.
- CS860158 — Inhibition of root elongation is similar to the wild type plants in the presence of various levels of exogenous cytokinin; root elongation in seedlings is inhibited by increasing concentrations of the cytokinin benzyl adenine (BA) in the growth media, showing a sharp decrease in root elongation between 10 and 50 nM BA.
- CS860159 — Inhibition of root elongation is substantially less sensitive to cytokinin than the wild type plants and is less responsive to cytokinin over the whole range of cytokinin benzyl adenine (BA) concentrations used in the growth media; roots are longer than those of wild-type plants when grown on near-saturating levels of cytokinin (10 uM BA), indicating a reduced response to cytokinin in addition to the reduced sensitivity; reduced sensitivity of lateral root formation to cytokinin; reduced inhibition of hypocotyl elongation in response to cytokinin, consistent with reduced sensitivity to the hormone.
- CS860160 — Inhibition of root elongation is substantially less sensitive to cytokinin than the wild type plants and is less responsive to cytokinin over the whole range of cytokinin benzyl adenine (BA) concentrations used in the growth media.
- CS860161 — Reduced inhibition of hypocotyl elongation in response to cytokinin, consistent with reduced sensitivity to the hormone; very short and narrow primary root; xylem development in the narrow primary root is less extensive than in the larger primary root of wild-type plants.
- CS860162 — Reduced inhibition of hypocotyl elongation in response to cytokinin, consistent with reduced sensitivity to the hormone; very short and narrow primary root; xylem development in the narrow primary root is less extensive than in the larger primary root of wild-type plants.
- CS860163 — Inhibition of root elongation is substantially less sensitive to cytokinin than the wild type plants and is less responsive to cytokinin over the whole range of cytokinin benzyl adenine (BA) concentrations used in the growth media; roots are longer than those of wild-type plants when grown on near-saturating levels of cytokinin (10 uM BA). It is not significantly different from the response of the ahp1,2,3 triple mutant, which indicates that AHP4 does not play a substantial role in this response; reduced inhibition of hypocotyl elongation in response to cytokinin, consistent with reduced sensitivity to the hormone.
- CS860164 — Abnormal growth and development: reduced vascular development in primary root; very short and narrow primary root; adventitious root development; smaller rosettes, shorter siliques, fewer seeds per silique; larger seeds and larger embryos than the wild type; reduced fertility; increased hypocotyl elongation in the presence of cytokinin.
Function-related keywords:
- whole plant , shoot axis apex , leaf lamina base , root , shoot system , plant embryo , vascular leaf , stamen , sepal , flower , inflorescence flower pedicel , cotyledon , petiole , hypocotyl , leaf apex , collective leaf structure , pollen
Literature:
- Histidine-containing phosphotransfer (HPt) signal transducers implicated in His-to-Asp phosphorelay in Arabidopsis. DOI: 10.1093/oxfordjournals.pcp.a029329 ; PMID: 10050311
- Characterization of genes for two-component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana. DOI: 10.1016/s0014-5793(98)01188-0 ; PMID: 9804162
- Two-component signal transduction pathways in Arabidopsis. DOI: 10.1104/pp.005504 ; PMID: 12068096
- The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling. DOI: 10.1105/tpc.106.045674 ; PMID: 17122069
- Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. DOI: 10.1111/j.1365-313X.2011.04637.x ; PMID: 21569135
- Silencing of meiosis-critical genes for engineering male sterility in plants. DOI: 10.1007/s00299-011-1193-9 ; PMID: 22120011
- Arabidopsis response Regulator1 and Arabidopsis histidine phosphotransfer Protein2 (AHP2), AHP3, and AHP5 function in cold signaling. DOI: 10.1104/pp.112.207621 ; PMID: 23124324
- Arabidopsis AHP2, AHP3, and AHP5 histidine phosphotransfer proteins function as redundant negative regulators of drought stress response. DOI: 10.1073/pnas.1302265110 ; PMID: 23487796
- Cytokinin antagonizes abscisic acid-mediated inhibition of cotyledon greening by promoting the degradation of abscisic acid insensitive5 protein in Arabidopsis. DOI: 10.1104/pp.113.234740 ; PMID: 24443524
- Involvement of two-component signalling systems in the regulation of stomatal aperture by light in Arabidopsis thaliana. DOI: 10.1111/nph.12813 ; PMID: 24758561
- AHP2, AHP3, and AHP5 act downstream of CKI1 in Arabidopsis female gametophyte development. DOI: 10.1093/jxb/erx181 ; PMID: 28633292
- High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1. DOI: 10.3390/plants9030304 ; PMID: 32121559
- Studies of cytokinin receptor-phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. DOI: 10.1071/FP16292 ; PMID: 32291033
- Root-derived trans-zeatin cytokinin protects Arabidopsis plants against photoperiod stress. DOI: 10.1111/pce.13860 ; PMID: 32716064
- Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis. DOI: 10.1073/pnas.2105021118 ; PMID: 34815339
- The histidine phosphotransfer AHP4 plays a negative role in Arabidopsis plant response to drought. DOI: 10.1111/tpj.15920 ; PMID: 35883014
- The type-B response regulators ARR10, ARR12, and ARR18 specify the central cell in Arabidopsis. DOI: 10.1093/plcell/koac285 ; PMID: 36130292
- Histone methylation readers MRG1/MRG2 interact with the transcription factor TCP14 to positively modulate cytokinin sensitivity in Arabidopsis. DOI: 10.1016/j.jgg.2023.02.011 ; PMID: 36870415
- Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system. DOI: 10.1016/s0014-5793(00)01860-3 ; PMID: 10930573
- Histidine-containing phosphotransfer (HPt) signal transducers implicated in His-to-Asp phosphorelay in Arabidopsis. DOI: 10.1093/oxfordjournals.pcp.a029329 ; PMID: 10050311
- Characterization of genes for two-component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana. DOI: 10.1016/s0014-5793(98)01188-0 ; PMID: 9804162
- Compilation and characterization of histidine-containing phosphotransmitters implicated in His-to-Asp phosphorelay in plants: AHP signal transducers of Arabidopsis thaliana. DOI: 10.1271/bbb.64.2486 ; PMID: 11193423
- The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling. DOI: 10.1105/tpc.106.045674 ; PMID: 17122069
- Cytokinin regulates type-A Arabidopsis Response Regulator activity and protein stability via two-component phosphorelay. DOI: 10.1105/tpc.107.052662 ; PMID: 18065689
- The Arabidopsis thaliana response regulator ARR22 is a putative AHP phospho-histidine phosphatase expressed in the chalaza of developing seeds. DOI: 10.1186/1471-2229-8-77 ; PMID: 18625081
- Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling. DOI: 10.1016/j.jprot.2017.06.004 ; PMID: 28627464
- The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling. DOI: 10.1105/tpc.106.045674 ; PMID: 17122069
Sequences:
cDNA Sequence
- >AT3G29350.1
TTTAAAGTAAAAAAATCTGGTAGCAGCGTTACAATTCGCGAGAAAGACAAAACACAAGTTTCTTCTTCTTGGGATTGGCTATTTCCAGAAATCCAAGTCAATAATCAAAGTCCAAACAAAAAAATCCTCTCCCAATCTCCGCTTCACTCTTCTCATGGACGCTCTCATTGCTCAGCTTCAGAGACAATTTCGTGATTACACCATTTCTCTCTACCAACAGGGGTTTTTGGATGATCAATTTACTGAGTTGAAAAAGCTACAAGATGATGGAAGTCCTGATTTTGTGTCTGAAGTGCTTTCACTTTTCTTTGAAGATTGTGTGAAGCTTATCAGTAACATGGCTAGAGCTTTGGACACGACAGGAACTGTAGATTTTAGTCAGGTAGGTGCTAGTGTGCATCAATTGAAGGGTAGTAGCTCAAGTGTTGGTGCCAAGAGGGTCAAAACTTTGTGTGTTAGCTTCAAGGAATGTTGTGAAGCTAAGAACTACGAAGGGTGTGTGAGATGTTTGCAGCAAGTGGATATTGAGTACAAGGCGTTAAAGACAAAGCTTCAAGATATGTTCAATCTTGAGAAACAGATCATTCAAGCTGGTGGTATAGTTCCTCAAGTGGATATTAACTAAAGAGACTAGTCCATAAGAAGAAAAAAGATGATGACTTTCTTTCTTTAGTTTCTCTTCTAAATTATTTTGGATTTGGTGTTTGCTCAAAAACTCAATAAAATATGTGCAAAAAGAAACAAAAACAAGTGATGGTTGTTTATAAATCAGTAGTATGTATTGTTTGATCTCATCCGAGAAAATTGAAACCATTGGACTAATGAATGTGATGATAATATATATTGGTTTGCTTCTGAAAGGTTTGGTTTGAGTAAGA - >AT3G29350.2
TTTAAAGTAAAAAAATCTGGTAGCAGCGTTACAATTCGCGAGAAAGACAAAACACAAGTTTCTTCTTCTTGGGATTGGCTATTTCCAGAAATCCAAGTCAATAATCAAAGTCCAAACAAAAAAATCCTCTCCCAATCTCCGCTTCACTCTTCTCATGGACGCTCTCATTGCTCAGCTTCAGAGACAATTTCGTGATTACACCATTTCTCTCTACCAACAGGGGTTTTTGGATGATCAATTTACTGAGTTGAAAAAGCTACAAGATGATGGAAGTCCTGATTTTGTGTCTGAAGTGCTTTCACTTTTCTTTGAAGATTGTGTGAAGCTTATCAGTAACATGGCTAGAGCTTTGGACACGACAGGAACTGTAGATTTTAGTCAGGTAGGTGCTAGTGTGCATCAATTGAAGGGTAGTAGCTCAAGTGTTGGTGCCAAGAGGGTCAAAACTTTGTGTGTTAGCTTCAAGGAATGTTGTGAAGCTAAGAACTACGAAGGGTGTGTGAGATGTTTGCAGCAAGTGGATATTGAGTACAAGGCGTTAAAGACAAAGCTTCAAGATATGTTCAATCTTGAGAAACAGATCATTCAAGCTGGTGGTATAGTTCCTCAAGTGGATATTAACTAAAGAGACTAGTCCATAAGAAGAAAAAAGATGATGACTTTCTTTCTTTAGTTTCTCTTCTAAATTATTTTGGATTTGGTGTTTGCTCAAAAACTCAATAAAATATGTGCAAAAAGAAACAAAAACAAGTGATGGTTGTTTATAAATCAGTAGTATGTATTGTTTGATCTCATCCGAGAAAATTGAAACCATTGGACTAATGAATGTGATGATAATATATATTGGTTTGCTTCTGAAAGGTTTGGTTTGAGTAAGA
CDS Sequence
- >AT3G29350.1
ATGGACGCTCTCATTGCTCAGCTTCAGAGACAATTTCGTGATTACACCATTTCTCTCTACCAACAGGGGTTTTTGGATGATCAATTTACTGAGTTGAAAAAGCTACAAGATGATGGAAGTCCTGATTTTGTGTCTGAAGTGCTTTCACTTTTCTTTGAAGATTGTGTGAAGCTTATCAGTAACATGGCTAGAGCTTTGGACACGACAGGAACTGTAGATTTTAGTCAGGTAGGTGCTAGTGTGCATCAATTGAAGGGTAGTAGCTCAAGTGTTGGTGCCAAGAGGGTCAAAACTTTGTGTGTTAGCTTCAAGGAATGTTGTGAAGCTAAGAACTACGAAGGGTGTGTGAGATGTTTGCAGCAAGTGGATATTGAGTACAAGGCGTTAAAGACAAAGCTTCAAGATATGTTCAATCTTGAGAAACAGATCATTCAAGCTGGTGGTATAGTTCCTCAAGTGGATATTAACTAA - >AT3G29350.2
ATGGACGCTCTCATTGCTCAGCTTCAGAGACAATTTCGTGATTACACCATTTCTCTCTACCAACAGGGGTTTTTGGATGATCAATTTACTGAGTTGAAAAAGCTACAAGATGATGGAAGTCCTGATTTTGTGTCTGAAGTGCTTTCACTTTTCTTTGAAGATTGTGTGAAGCTTATCAGTAACATGGCTAGAGCTTTGGACACGACAGGAACTGTAGATTTTAGTCAGGTAGGTGCTAGTGTGCATCAATTGAAGGGTAGTAGCTCAAGTGTTGGTGCCAAGAGGGTCAAAACTTTGTGTGTTAGCTTCAAGGAATGTTGTGAAGCTAAGAACTACGAAGGGTGTGTGAGATGTTTGCAGCAAGTGGATATTGAGTACAAGGCGTTAAAGACAAAGCTTCAAGATATGTTCAATCTTGAGAAACAGATCATTCAAGCTGGTGGTATAGTTCCTCAAGTGGATATTAACTAA
Protein Sequence
- >AT3G29350.1
MDALIAQLQRQFRDYTISLYQQGFLDDQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARALDTTGTVDFSQVGASVHQLKGSSSSVGAKRVKTLCVSFKECCEAKNYEGCVRCLQQVDIEYKALKTKLQDMFNLEKQIIQAGGIVPQVDIN - >AT3G29350.2
MDALIAQLQRQFRDYTISLYQQGFLDDQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARALDTTGTVDFSQVGASVHQLKGSSSSVGAKRVKTLCVSFKECCEAKNYEGCVRCLQQVDIEYKALKTKLQDMFNLEKQIIQAGGIVPQVDIN