Gene Details:

  • Gene ID: AT3G29320
  • Gene Symbol: PHS1
  • Gene Name: alpha-glucan phosphorylase 1
  • Description: Glycosyl transferase, family 35;(source:Araport11)
  • TAIR Accession: locus:2093787
  • Genome: Araport11_genome_release
  • Species: Arabidopsis thaliana

Transcripts:

Plant Ontology Annotations:

  • PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
  • PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
  • PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
  • PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
  • PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
  • PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
  • PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
  • PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
  • PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
  • PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
  • PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
  • PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
  • PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
  • PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
  • PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
  • PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
  • PO:0000293  — guard cell — célula guardiana (Spanish, exact), occlusive cell (exact), 孔辺細胞 (Japanese, exact)

Gene Ontology:

  • GO:0009536  — located in — plastid
  • GO:0030170  — enables — pyridoxal phosphate binding
  • GO:0005737  — is active in — cytoplasm
  • GO:0009507  — located in — chloroplast
  • GO:0009414  — acts upstream of or within — response to water deprivation
  • GO:0005829  — located in — cytosol
  • GO:0009570  — located in — chloroplast stroma
  • GO:0102250  — enables — linear malto-oligosaccharide phosphorylase activity
  • GO:0102499  — enables — SHG alpha-glucan phosphorylase activity
  • GO:0004645  — enables — 1,4-alpha-oligoglucan phosphorylase activity
  • GO:0008184  — enables — glycogen phosphorylase activity
  • GO:0005980  — involved in — glycogen catabolic process
  • GO:0009266  — acts upstream of or within — response to temperature stimulus
  • GO:0016757  — enables — glycosyltransferase activity

Germplasm Phenotype:

  • atphs1-1  — The leaves of mature mutant plants consistently had small, white lesions on the tips or margins of fully expanded leaves. The lesions did not increase in size after appearance and were not bordered by chlorotic tissue. The frequency and severity of lesions varied between batches of plants, but lesion-free mutant plants were rare. Further examination of the lesions on leaves of the mutant plants revealed that high levels of starch were present in the living cells bordering each lesion at the end of the night, when the rest of the leaf had metabolized its starch. This starch accumulation occurred in a highly cell-specific manner with adjacent starch-rich and starch-free cells.
  • atphs1-2  — The average midday activity of hexokinase (HXK) was 20% higher in the mutant leaves, statistically significant at the P = 0.1 level of confidence.
  • atphs1-2  — The leaves of mature mutant plants consistently had small, white lesions on the tips or margins of fully expanded leaves. The lesions did not increase in size after appearance and were not bordered by chlorotic tissue. The frequency and severity of lesions varied between batches of plants, but lesion-free mutant plants were rare. Further examination of the lesions on leaves of the mutant plants revealed that high levels of starch were present in the living cells bordering each lesion at the end of the night, when the rest of the leaf had metabolized its starch. This starch accumulation occurred in a highly cell-specific manner with adjacent starch-rich and starch-free cells.
  • atphs1-2  — The midday and midnight activities of DPE1, DPE2, β-amylase, and phosphoglucomutase (PGM) were not changed in the atphs2-1 leaves relative to wild-type leaves.

Literature:

Sequences:

cDNA Sequence
  • >AT3G29320.1
    TGAAACTGAAAAAAAGCTTGAACACTCCATTGTTCTCTACTCTACCACTATCTCACAGAGATTCATGGATACGATGCGAATCTCCGGTGTATCAACCGGAGCTGAGGTTTTAATACAATGCAATTCCTTATCAAGCCTCGTTTCTCGTCGTTGCGACGACGGAAAATGGCGAACGAGAATGTTTCCGGCGAGAAACAGAGACTTGCGTCCATCGCCGACGAGAAGATCCTTTTTGTCGGTGAAATCTATCTCTAGCGAACCGAAAGCCAAAGTAACCGACGCAGTTCTCGATTCCGAACAAGAAGTGTTTATTAGCTCGATGAATCCGTTTGCGCCAGATGCTGCTTCGGTAGCTTCGAGTATCAAGTACCACGCGGAGTTTACGCCATTGTTTTCACCGGAGAAGTTTGAGTTGCCAAAGGCGTTCTTTGCGACTGCGCAAAGTGTTAGAGATGCTTTGATCATGAATTGGAATGCAACTTATGAGTATTACAACAGAGTGAATGTGAAACAAGCGTATTATTTGTCAATGGAGTTTTTGCAGGGTAGAGCCTTATCGAATGCCGTGGGTAACCTTGGGCTTAATAGCGCTTATGGTGATGCTTTGAAGAGGCTTGGTTTTGATTTGGAAAGCGTGGCTAGTCAGGAGCCAGATCCTGCACTTGGGAATGGTGGACTCGGGAGACTTGCCTCGTGTTTTTTGGATTCCATGGCAACTTTGAATTATCCGGCTTGGGGTTATGGACTTAGATACAAGTATGGCTTGTTCAAACAGAGAATTACAAAAGATGGACAGGAGGAAGCTGCAGAAGATTGGCTTGAGCTAAGCAATCCTTGGGAAATAGTCAGAAATGATGTCTCATATCCTATTAAGTTCTATGGGAAAGTGGTTTTTGGATCAGATGGTAAGAAACGGTGGATTGGTGGAGAAGACATTGTTGCTGTTGCTTATGATGTTCCTATACCTGGTTATAAAACTAAGACAACTATCAATCTGCGGCTCTGGTCAACAAAAGCTCCTTCCGAAGATTTTGATTTATCTTCATATAACTCTGGGAAGCATACTGAGGCAGCAGAAGCTCTATTCAACGCTGAAAAGATTTGCTTCGTGCTTTACCCCGGAGATGAGTCAACTGAAGGAAAGGCTCTTCGTCTGAAGCAACAATACACTCTGTGCTCAGCCTCGCTACAAGATATCGTAGCACGTTTTGAGACAAGGTCTGGAGGAAACGTCAACTGGGAAGAATTTCCAGAGAAGGTTGCAGTGCAGATGAATGACACTCACCCTACCCTATGCATTCCTGAGCTAATGAGGATTCTAATGGATTTAAAAGGACTAAGCTGGGAAGACGCTTGGAAAATCACACAAAGGACTGTGGCATACACAAACCATACAGTCTTGCCTGAGGCACTGGAGAAGTGGAGTTTAGAACTCATGGAGAAATTGCTTCCTCGTCATGTGGAGATTATCGAAAAGATTGATGAGGAGCTAGTTCGCACAATTGTTTCAGAGTATGGCACCGCGGATCCTGACTTACTTGAAGAAAAACTGAAGGCAATGAGGATCTTGGAAAATGTCGAGTTGCCTTCTGCCTTTGCAGATGTGATCGTGAAGCCGGTGAACAAACCAGTTACTGCAAAAGATGCTCAAAATGGCGTGAAAACGGAACAAGAAGAGGAAAAAACTGCTGGAGAGGAAGAGGAAGACGAAGTTATCCCAGAACCAACAGTAGAACCCCCCAAGATGGTCCGTATGGCCAACCTTGCTGTTGTGGGTGGTCATGCTGTAAATGGCGTTGCAGAGATACACAGTGAAATAGTGAAGCAGGACGTGTTTAATGATTTCGTACAGTTGTGGCCAGAAAAATTTCAGAACAAAACAAATGGAGTAACACCAAGGCGATGGATTCGTTTTTGCAACCCATATTTAAGTGATATTATAACTAACTGGATAGGCACAGAAGACTGGGTCTTAAATACCGAAAAGGTTGCGGAACTAAGAAAGTTTGCAGATAATGAAGATCTCCAATCTGAGTGGAGGGCAGCAAAGAAGAAGAACAAGTTGAAGGTTGTATCACTTATCAAGGAAAGAACTGGATATACTGTCAGCCCCGATGCAATGTTCGACATTCAGATCAAGCGTATACATGAGTACAAGCGACAACTGCTAAATATCTTGGGAATTGTTTACCGCTACAAAAAGATGAAGGAAATGAGTGCTAGTGAGAGAGAGAAAGCATTTGTTCCAAGAGTTTGCATATTTGGGGGAAAAGCATTTGCCACATATGTGCAAGCTAAGAGAATTGTTAAATTTATCACAGATGTTGCGTCTACAATTAACCATGATCCAGAAATAGGTGACCTCCTTAAGGTTATCTTTGTTCCTGATTACAATGTCAGTGTTGCTGAATTGCTCATTCCAGCAAGTGAGCTTTCTCAGCACATCAGTACTGCTGGGATGGAAGCTAGTGGGACAAGCAACATGAAATTTTCGATGAACGGTTGCGTTTTGATTGGAACCTTGGATGGGGCGAATGTCGAGATTAGAGAAGAAGTTGGAGAAGAAAATTTCTTCCTCTTTGGTGCCAAAGCTGATCAGATTGTGAACCTCAGGAAGGAGAGAGCAGAGGGAAAGTTTGTTCCCGATCCTACTTTTGAAGAAGTCAAGAAGTTCGTTGGAAGCGGCGTCTTTGGCTCAAATAGCTATGATGAACTAATCGGCTCTTTGGAAGGAAACGAAGGCTTTGGACGAGCGGATTACTTCCTAGTTGGCAAAGACTTTCCTAGTTACATCGAATGCCAAGAAAAAGTCGACGAGGCATACCGAGACCAGAAAAGATGGACGAGAATGTCAATAATGAACACAGCAGGTTCATTCAAGTTTAGCAGTGACCGGACGATCCACGAATACGCCAAAGACATATGGAATATTAAGCAAGTGGAACTTCCATGAGCCTTGAGACAAGTCTACCTTAAGAACTCTTTTGTACGTCCCATGTTTCTAATGTAATCAAGGTAATAAGGAAATCATGTAATGATGCAATACAATGAACGTAAGAAATAAGATTCACGAGTCAATGTAATTTACAAGGGCCTTCGCTGCCTTAGCGGCGTGCTTCTGACCGGCTAAATGATCGTTGTAAACAATGTCGCTATTACAAACTACGTTGCATATTCTAC
CDS Sequence
  • >AT3G29320.1
    ATGGATACGATGCGAATCTCCGGTGTATCAACCGGAGCTGAGGTTTTAATACAATGCAATTCCTTATCAAGCCTCGTTTCTCGTCGTTGCGACGACGGAAAATGGCGAACGAGAATGTTTCCGGCGAGAAACAGAGACTTGCGTCCATCGCCGACGAGAAGATCCTTTTTGTCGGTGAAATCTATCTCTAGCGAACCGAAAGCCAAAGTAACCGACGCAGTTCTCGATTCCGAACAAGAAGTGTTTATTAGCTCGATGAATCCGTTTGCGCCAGATGCTGCTTCGGTAGCTTCGAGTATCAAGTACCACGCGGAGTTTACGCCATTGTTTTCACCGGAGAAGTTTGAGTTGCCAAAGGCGTTCTTTGCGACTGCGCAAAGTGTTAGAGATGCTTTGATCATGAATTGGAATGCAACTTATGAGTATTACAACAGAGTGAATGTGAAACAAGCGTATTATTTGTCAATGGAGTTTTTGCAGGGTAGAGCCTTATCGAATGCCGTGGGTAACCTTGGGCTTAATAGCGCTTATGGTGATGCTTTGAAGAGGCTTGGTTTTGATTTGGAAAGCGTGGCTAGTCAGGAGCCAGATCCTGCACTTGGGAATGGTGGACTCGGGAGACTTGCCTCGTGTTTTTTGGATTCCATGGCAACTTTGAATTATCCGGCTTGGGGTTATGGACTTAGATACAAGTATGGCTTGTTCAAACAGAGAATTACAAAAGATGGACAGGAGGAAGCTGCAGAAGATTGGCTTGAGCTAAGCAATCCTTGGGAAATAGTCAGAAATGATGTCTCATATCCTATTAAGTTCTATGGGAAAGTGGTTTTTGGATCAGATGGTAAGAAACGGTGGATTGGTGGAGAAGACATTGTTGCTGTTGCTTATGATGTTCCTATACCTGGTTATAAAACTAAGACAACTATCAATCTGCGGCTCTGGTCAACAAAAGCTCCTTCCGAAGATTTTGATTTATCTTCATATAACTCTGGGAAGCATACTGAGGCAGCAGAAGCTCTATTCAACGCTGAAAAGATTTGCTTCGTGCTTTACCCCGGAGATGAGTCAACTGAAGGAAAGGCTCTTCGTCTGAAGCAACAATACACTCTGTGCTCAGCCTCGCTACAAGATATCGTAGCACGTTTTGAGACAAGGTCTGGAGGAAACGTCAACTGGGAAGAATTTCCAGAGAAGGTTGCAGTGCAGATGAATGACACTCACCCTACCCTATGCATTCCTGAGCTAATGAGGATTCTAATGGATTTAAAAGGACTAAGCTGGGAAGACGCTTGGAAAATCACACAAAGGACTGTGGCATACACAAACCATACAGTCTTGCCTGAGGCACTGGAGAAGTGGAGTTTAGAACTCATGGAGAAATTGCTTCCTCGTCATGTGGAGATTATCGAAAAGATTGATGAGGAGCTAGTTCGCACAATTGTTTCAGAGTATGGCACCGCGGATCCTGACTTACTTGAAGAAAAACTGAAGGCAATGAGGATCTTGGAAAATGTCGAGTTGCCTTCTGCCTTTGCAGATGTGATCGTGAAGCCGGTGAACAAACCAGTTACTGCAAAAGATGCTCAAAATGGCGTGAAAACGGAACAAGAAGAGGAAAAAACTGCTGGAGAGGAAGAGGAAGACGAAGTTATCCCAGAACCAACAGTAGAACCCCCCAAGATGGTCCGTATGGCCAACCTTGCTGTTGTGGGTGGTCATGCTGTAAATGGCGTTGCAGAGATACACAGTGAAATAGTGAAGCAGGACGTGTTTAATGATTTCGTACAGTTGTGGCCAGAAAAATTTCAGAACAAAACAAATGGAGTAACACCAAGGCGATGGATTCGTTTTTGCAACCCATATTTAAGTGATATTATAACTAACTGGATAGGCACAGAAGACTGGGTCTTAAATACCGAAAAGGTTGCGGAACTAAGAAAGTTTGCAGATAATGAAGATCTCCAATCTGAGTGGAGGGCAGCAAAGAAGAAGAACAAGTTGAAGGTTGTATCACTTATCAAGGAAAGAACTGGATATACTGTCAGCCCCGATGCAATGTTCGACATTCAGATCAAGCGTATACATGAGTACAAGCGACAACTGCTAAATATCTTGGGAATTGTTTACCGCTACAAAAAGATGAAGGAAATGAGTGCTAGTGAGAGAGAGAAAGCATTTGTTCCAAGAGTTTGCATATTTGGGGGAAAAGCATTTGCCACATATGTGCAAGCTAAGAGAATTGTTAAATTTATCACAGATGTTGCGTCTACAATTAACCATGATCCAGAAATAGGTGACCTCCTTAAGGTTATCTTTGTTCCTGATTACAATGTCAGTGTTGCTGAATTGCTCATTCCAGCAAGTGAGCTTTCTCAGCACATCAGTACTGCTGGGATGGAAGCTAGTGGGACAAGCAACATGAAATTTTCGATGAACGGTTGCGTTTTGATTGGAACCTTGGATGGGGCGAATGTCGAGATTAGAGAAGAAGTTGGAGAAGAAAATTTCTTCCTCTTTGGTGCCAAAGCTGATCAGATTGTGAACCTCAGGAAGGAGAGAGCAGAGGGAAAGTTTGTTCCCGATCCTACTTTTGAAGAAGTCAAGAAGTTCGTTGGAAGCGGCGTCTTTGGCTCAAATAGCTATGATGAACTAATCGGCTCTTTGGAAGGAAACGAAGGCTTTGGACGAGCGGATTACTTCCTAGTTGGCAAAGACTTTCCTAGTTACATCGAATGCCAAGAAAAAGTCGACGAGGCATACCGAGACCAGAAAAGATGGACGAGAATGTCAATAATGAACACAGCAGGTTCATTCAAGTTTAGCAGTGACCGGACGATCCACGAATACGCCAAAGACATATGGAATATTAAGCAAGTGGAACTTCCATGA
Protein Sequence
  • >AT3G29320.1
    MDTMRISGVSTGAEVLIQCNSLSSLVSRRCDDGKWRTRMFPARNRDLRPSPTRRSFLSVKSISSEPKAKVTDAVLDSEQEVFISSMNPFAPDAASVASSIKYHAEFTPLFSPEKFELPKAFFATAQSVRDALIMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGLNSAYGDALKRLGFDLESVASQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWLELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVAVAYDVPIPGYKTKTTINLRLWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEKICFVLYPGDESTEGKALRLKQQYTLCSASLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKIDEELVRTIVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAKDAQNGVKTEQEEEKTAGEEEEDEVIPEPTVEPPKMVRMANLAVVGGHAVNGVAEIHSEIVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAELRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFDIQIKRIHEYKRQLLNILGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEIGDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGANVEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSNSYDELIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDRTIHEYAKDIWNIKQVELP