Gene Details:
- Gene ID: AT3G26810
- Gene Symbol: AFB2
- Gene Name: auxin signaling F-box 2
- Description: auxin signaling F-box 2;(source:Araport11)
- TAIR Accession: locus:2088464
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0000037 — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
- PO:0008019 — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
- PO:0009005 — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
- PO:0009006 — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
- PO:0009009 — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
- PO:0009025 — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
- PO:0009029 — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
- PO:0009031 — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
- PO:0009046 — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
- PO:0009052 — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
- PO:0020030 — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
- PO:0020038 — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
- PO:0020100 — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
- PO:0020137 — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
- PO:0025022 — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
- PO:0009066 — anther — antera (Spanish, exact), 半葯,花粉のう (Japanese, exact), Poaceae anther (narrow), Zea anther (narrow)
- PO:0009067 — filament — filamento (Spanish, exact), 花糸 (Japanese, exact), Poaceae filament (narrow), Zea filament (narrow)
- PO:0025195 — pollen tube cell — célula del tubo polínico (Spanish, exact), 花粉管細胞 (Japanese, exact)
Gene Ontology:
- GO:0010011 — enables — auxin binding
- GO:0009734 — involved in — auxin-activated signaling pathway
- GO:0048443 — acts upstream of or within — stamen development
- GO:0009734 — acts upstream of or within — auxin-activated signaling pathway
- GO:0019005 — part of — SCF ubiquitin ligase complex
- GO:0005737 — located in — cytoplasm
- GO:0010152 — acts upstream of or within — pollen maturation
- GO:0000325 — located in — plant-type vacuole
- GO:0005515 — enables — protein binding
- GO:0000822 — enables — inositol hexakisphosphate binding
- GO:0031146 — involved in — SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Germplasm Phenotype:
- CS72715 — Plants that are hemizygous for the complementing transgene are shorter and slightly less fertile than WT. Plants homozygous for it are closer to WT in stature and fertility. Hemizygous plants siliques contain roughly 75% viable embryos expressing mCitrine (and mCherry & mOrange2) and the other 25% lack fluorescence and display embryo defects at the 16-cell stage or earlier. AT3G26810 CS72716 Plants that are hemizygous for the complementing transgene are shorter and slightly less fertile than WT. Plants homozygous for it are closer to WT in stature and fertility. Hemizygous plants siliques contain roughly 75% viable embryos expressing mCitrine (and mCherry & mOrange2) and the other 25% lack fluorescence and display embryo defects at the 16-cell stage or earlier.
- CS72719 — Rosettes are significantly smaller and inflorescences are substantially shorter and more branched than those of WT. Some siliques have incomplete valves.
- CS72720 — Rosettes are significantly smaller and inflorescences are substantially shorter and more branched than those of WT. Some siliques have incomplete valves.
- CS72725 — more YFP signal than AFB2-Venus (CS69662)
Function-related keywords:
- shoot axis apex , leaf lamina base , root , shoot system , plant embryo , vascular leaf , stamen , sepal , flower , inflorescence flower pedicel , cotyledon , petiole , hypocotyl , leaf apex , collective leaf structure , pollen , anther , filament , pollen tube cell , pollen
Literature:
- Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. DOI: 10.1105/tpc.104.022830 ; PMID: 15258262
- Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. DOI: 10.1186/gb-2004-5-9-r65 ; PMID: 15345049
- Computational prediction of miRNAs in Arabidopsis thaliana. DOI: 10.1101/gr.2908205 ; PMID: 15632092
- Regulation of genes associated with auxin, ethylene and ABA pathways by 2,4-dichlorophenoxyacetic acid in Arabidopsis. DOI: 10.1007/s10142-005-0012-1 ; PMID: 16317577
- A transcriptome-based characterization of habituation in plant tissue culture. DOI: 10.1104/pp.105.076059 ; PMID: 16489130
- Evolution of Arabidopsis microRNA families through duplication events. DOI: 10.1101/gr.4680506 ; PMID: 16520461
- Plant development is regulated by a family of auxin receptor F box proteins. DOI: 10.1016/j.devcel.2005.05.014 ; PMID: 15992545
- MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis. DOI: 10.1105/tpc.106.042127 ; PMID: 16798886
- The F-box protein TIR1 is an auxin receptor. DOI: 10.1038/nature03543 ; PMID: 15917797
- Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development. DOI: 10.1105/tpc.105.040675 ; PMID: 17435085
- Evolution of complexity in miRNA-mediated gene regulation systems. DOI: 10.1016/j.tig.2007.11.002 ; PMID: 18192068
- Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. DOI: 10.1104/pp.108.126375 ; PMID: 18775970
- Phosphate availability alters lateral root development in Arabidopsis by modulating auxin sensitivity via a mechanism involving the TIR1 auxin receptor. DOI: 10.1105/tpc.108.058719 ; PMID: 19106375
- Complex regulation of the TIR1/AFB family of auxin receptors. DOI: 10.1073/pnas.0911967106 ; PMID: 20018756
- Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis. DOI: 10.1007/s11103-010-9667-7 ; PMID: 20661628
- Auxin and salicylic acid signalings counteract the regulation of adaptive responses to stress. DOI: 10.4161/psb.6.3.14676 ; PMID: 21358272
- Mutant analysis in Arabidopsis provides insight into the molecular mode of action of the auxinic herbicide dicamba. DOI: 10.1371/journal.pone.0017245 ; PMID: 21408147
- Transkingdom signaling based on bacterial cyclodipeptides with auxin activity in plants. DOI: 10.1073/pnas.1006740108 ; PMID: 21482761
- Apoplastic reactive oxygen species transiently decrease auxin signaling and cause stress-induced morphogenic response in Arabidopsis. DOI: 10.1104/pp.111.181883 ; PMID: 22007024
- Regulation of auxin response by miR393-targeted transport inhibitor response protein 1 is involved in normal development in Arabidopsis. DOI: 10.1007/s11103-011-9838-1 ; PMID: 22042293
- The jasmonate receptor COI1 plays a role in jasmonate-induced lateral root formation and lateral root positioning in Arabidopsis thaliana. DOI: 10.1016/j.jplph.2012.05.002 ; PMID: 22658222
- A comprehensive analysis of interaction and localization of Arabidopsis SKP1-like (ASK) and F-box (FBX) proteins. DOI: 10.1371/journal.pone.0050009 ; PMID: 23166809
- A plant microRNA regulates the adaptation of roots to drought stress. DOI: 10.1016/j.febslet.2012.05.013 ; PMID: 22613571
- Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis. DOI: 10.1111/jipb.12302 ; PMID: 25329496
- Endogenous Arabidopsis messenger RNAs transported to distant tissues. DOI: 10.1038/nplants.2015.25 ; PMID: 27247031
- NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development. DOI: 10.1371/journal.pgen.1005923 ; PMID: 26942722
- The Arabidopsis Auxin Receptor F-Box Proteins AFB4 and AFB5 Are Required for Response to the Synthetic Auxin Picloram. DOI: 10.1534/g3.115.025585 ; PMID: 26976444
- miR393 contributes to the embryogenic transition induced in vitro in Arabidopsis via the modification of the tissue sensitivity to auxin treatment. DOI: 10.1007/s00425-016-2505-7 ; PMID: 27040841
- N,N-dimethyl hexadecylamine and related amines regulate root morphogenesis via jasmonic acid signaling in Arabidopsis thaliana. DOI: 10.1007/s00709-016-1031-6 ; PMID: 27696021
- Folic acid orchestrates root development linking cell elongation with auxin response and acts independently of the TARGET OF RAPAMYCIN signaling in Arabidopsis thaliana. DOI: 10.1016/j.plantsci.2017.09.011 ; PMID: 28969797
- Jasmonic Acid Inhibits Auxin-Induced Lateral Rooting Independently of the CORONATINE INSENSITIVE1 Receptor. DOI: 10.1104/pp.18.00357 ; PMID: 29934297
- Regulation of SCF(TIR1/AFBs) E3 ligase assembly by S-nitrosylation of Arabidopsis SKP1-like1 impacts on auxin signaling. DOI: 10.1016/j.redox.2018.07.003 ; PMID: 30031268
- Modulation of Auxin Signaling and Development by Polyadenylation Machinery. DOI: 10.1104/pp.18.00782 ; PMID: 30487141
- miR393s regulate salt stress response pathway in Arabidopsis thaliana through scaffold protein RACK1A mediated ABA signaling pathways. DOI: 10.1080/15592324.2019.1600394 ; PMID: 31021701
- Jasmonate and auxin perception: how plants keep F-boxes in check. DOI: 10.1093/jxb/erz272 ; PMID: 31173086
- Reactive oxygen species and reactive carbonyl species constitute a feed-forward loop in auxin signaling for lateral root formation. DOI: 10.1111/tpj.14456 ; PMID: 31306517
- A Molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAA-Dependent Auxin Signaling in Arabidopsis. DOI: 10.1016/j.molp.2019.09.001 ; PMID: 31520787
- The role of auxin transporters and receptors in adventitious rooting of Arabidopsis thaliana pre-etiolated flooded seedlings. DOI: 10.1016/j.plantsci.2019.110294 ; PMID: 31779904
- The growth of Arabidopsis primary root is repressed by several and diverse amino acids through auxin-dependent and independent mechanisms and MPK6 kinase activity. DOI: 10.1016/j.plantsci.2020.110717 ; PMID: 33288023
- A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception. DOI: 10.1093/plphys/kiab269 ; PMID: 34618142
- The Photoperiod Stress Response in Arabidopsis thaliana Depends on Auxin Acting as an Antagonist to the Protectant Cytokinin. DOI: 10.3390/ijms23062936 ; PMID: 35328357
- A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. DOI: 10.1126/science.1126088 ; PMID: 16627744
- A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture. DOI: 10.1074/mcp.M600250-MCP200 ; PMID: 17151019
- Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. DOI: 10.1105/tpc.107.057570 ; PMID: 18628351
Sequences:
cDNA Sequence
- >AT3G26810.1
GCCATTTCCCATCAACGGTCAAGATCCATCTCCTCTTCTTCTCCTTCAAACCATTCCCCACCGTTAGATCATCTCCAAACCTAACCACGGTTCATTCTTTAACCACGGTTTGTTTCGGTCAGCCAGGTAGGGGCCTCTCTGATGTCCTTCAAGACTTTTTATTTTTCTCCATTACCAAAGTCACCAAGAAGCCAGAAGAAAGAGAATAGAGAGAGAGAGAGAGTTTTGTTCTTCTTCTTTCTGGTTCCTTTGCTTTGCTTTGGTTGCGTGATCTCCTCTCTCATCAATCTTCTTCATCATCATTCTCATCATCATTAGCTTCTTGATTTTTTTTTCCTTCTTTTTTTCGTTTCAAGAAACTAAACAAAGTTTCTATTTATGGTTTTCTGATTCTTCTTCTACAGATCTATTATTTGATTCTCCTTCGATCATTGTCAACGTTTTCTTCTTTTGTAATCATCACAGTTTTTTTTGAGAATTTGTTCATTTTTTTTTCTCATATATTGATTCGAGTTCTTTCTTCTTGAATCTTGTTCTTCATAGATCTAGTTTTTGTTTTTTACTCTCTAGATCTATAGATCTGTTGAATTCACTTCTTTAATCCATCTCTGTGTTCTTGAGTTCCTATGGTCTAAGTCAAACTCAAGTTCAAGATCTGTTGAATTTGGAATCTTGCTGGTGAAGTTAGAGATGAATTATTTCCCAGATGAAGTAATAGAGCATGTATTCGACTTTGTAACATCTCACAAAGACAGGAATGCTATATCTCTTGTATGCAAATCATGGTACAAGATTGAAAGATACAGTAGGCAAAAGGTTTTCATTGGAAACTGTTATGCCATTAATCCAGAGAGGTTGCTTCGGAGATTCCCATGTCTAAAGTCTTTGACTTTGAAAGGAAAACCTCATTTTGCGGATTTCAATTTGGTTCCTCATGAATGGGGAGGTTTTGTGCTACCTTGGATTGAGGCTTTGGCTAGAAGCCGTGTAGGACTTGAAGAGCTTAGGTTGAAGAGGATGGTTGTTACTGATGAGAGTCTTGAGCTGCTTTCTCGTTCTTTTGTCAATTTTAAGTCTTTGGTCCTTGTTAGCTGTGAAGGTTTTACCACTGATGGTCTTGCCTCTATTGCCGCTAATTGCAGGCATCTTCGGGATCTTGATTTGCAAGAGAATGAAATCGATGATCATAGAGGTCAATGGTTAAGTTGTTTCCCAGACACTTGCACGACTCTTGTCACGCTAAACTTTGCGTGCCTCGAAGGAGAAACTAATCTGGTTGCTCTAGAGAGGCTTGTTGCTAGGTCTCCAAACCTAAAGAGTCTGAAGCTAAATCGTGCAGTACCGTTAGATGCACTCGCAAGGTTAATGGCGTGTGCGCCGCAGATAGTTGACTTAGGAGTAGGGTCTTATGAGAATGACCCAGATTCCGAGTCTTACTTGAAACTCATGGCTGTCATAAAGAAATGCACCTCGTTGAGGAGTTTGTCGGGTTTTCTAGAGGCTGCTCCTCACTGTCTCTCAGCTTTCCACCCAATATGTCATAACCTCACCTCCTTGAATCTTAGTTACGCAGCTGAGATTCATGGTAGCCACCTTATTAAGCTTATTCAGCATTGCAAGAAACTTCAGCGGTTATGGATTTTGGATAGTATAGGTGACAAAGGGCTTGAAGTTGTAGCTTCTACATGTAAAGAGTTACAAGAGCTTAGGGTTTTTCCATCTGATTTACTCGGTGGAGGCAACACAGCTGTGACCGAAGAAGGTCTAGTTGCCATCTCGGCAGGCTGCCCTAAGCTCCACTCTATACTCTACTTCTGCCAACAAATGACAAACGCAGCTCTCGTAACCGTTGCCAAGAACTGTCCAAATTTCATCCGTTTCCGACTCTGCATCCTCGAGCCAAACAAACCCGATCACGTCACATCTCAACCTCTAGACGAAGGCTTTGGAGCAATCGTCAAAGCCTGCAAGAGCCTGAGAAGGCTTTCTCTCTCAGGTCTCCTTACAGACCAAGTCTTCCTCTACATCGGAATGTACGCGAATCAGCTCGAGATGCTCTCCATAGCCTTTGCAGGAGATACAGACAAAGGCATGCTATATGTGTTGAATGGTTGCAAAAAGATGAAGAAACTAGAGATAAGGGATAGTCCGTTTGGGGACACGGCGCTTCTTGCTGATGTGAGCAAGTATGAAACAATGCGATCCCTTTGGATGTCTTCATGTGAAGTCACACTCAGTGGATGCAAAAGGCTCGCAGAGAAAGCGCCATGGCTCAATGTAGAGATCATAAACGAGAATGATAATAACCGGATGGAAGAAAACGGACACGAGGGGAGGCAGAAAGTGGATAAGTTGTATCTGTACCGGACTGTGGTTGGGACAAGAATGGATGCGCCGCCATTTGTGTGGATTCTCTAAGTCCTTTTCAGACCAAACTGGTTCTGATTGGTCGTCTTCCGTTAGTGTGTCTTTCCCGAATTAGGTGTACTTTTTGTTTTCTTTAAATTAAGACAAGAACTGATTCTTCTGTGAAAGCCATTCTGTTTTTGTTTTTGACTTTCCTCCGTTGATTTATTGGTTTCTTTTTTCAAGTTATTACAAAGTGCTACGGTTTGTCTTATTTTTCTAAGCTTTTTAGATTTACCTTGCTAGGAAACAAGAATGTACACAATAAAGAAAAAAACATGTACATAATGTAATATGTTAATAGAATCAAGTAATCATAGATTTGAGACT
CDS Sequence
- >AT3G26810.1
ATGAATTATTTCCCAGATGAAGTAATAGAGCATGTATTCGACTTTGTAACATCTCACAAAGACAGGAATGCTATATCTCTTGTATGCAAATCATGGTACAAGATTGAAAGATACAGTAGGCAAAAGGTTTTCATTGGAAACTGTTATGCCATTAATCCAGAGAGGTTGCTTCGGAGATTCCCATGTCTAAAGTCTTTGACTTTGAAAGGAAAACCTCATTTTGCGGATTTCAATTTGGTTCCTCATGAATGGGGAGGTTTTGTGCTACCTTGGATTGAGGCTTTGGCTAGAAGCCGTGTAGGACTTGAAGAGCTTAGGTTGAAGAGGATGGTTGTTACTGATGAGAGTCTTGAGCTGCTTTCTCGTTCTTTTGTCAATTTTAAGTCTTTGGTCCTTGTTAGCTGTGAAGGTTTTACCACTGATGGTCTTGCCTCTATTGCCGCTAATTGCAGGCATCTTCGGGATCTTGATTTGCAAGAGAATGAAATCGATGATCATAGAGGTCAATGGTTAAGTTGTTTCCCAGACACTTGCACGACTCTTGTCACGCTAAACTTTGCGTGCCTCGAAGGAGAAACTAATCTGGTTGCTCTAGAGAGGCTTGTTGCTAGGTCTCCAAACCTAAAGAGTCTGAAGCTAAATCGTGCAGTACCGTTAGATGCACTCGCAAGGTTAATGGCGTGTGCGCCGCAGATAGTTGACTTAGGAGTAGGGTCTTATGAGAATGACCCAGATTCCGAGTCTTACTTGAAACTCATGGCTGTCATAAAGAAATGCACCTCGTTGAGGAGTTTGTCGGGTTTTCTAGAGGCTGCTCCTCACTGTCTCTCAGCTTTCCACCCAATATGTCATAACCTCACCTCCTTGAATCTTAGTTACGCAGCTGAGATTCATGGTAGCCACCTTATTAAGCTTATTCAGCATTGCAAGAAACTTCAGCGGTTATGGATTTTGGATAGTATAGGTGACAAAGGGCTTGAAGTTGTAGCTTCTACATGTAAAGAGTTACAAGAGCTTAGGGTTTTTCCATCTGATTTACTCGGTGGAGGCAACACAGCTGTGACCGAAGAAGGTCTAGTTGCCATCTCGGCAGGCTGCCCTAAGCTCCACTCTATACTCTACTTCTGCCAACAAATGACAAACGCAGCTCTCGTAACCGTTGCCAAGAACTGTCCAAATTTCATCCGTTTCCGACTCTGCATCCTCGAGCCAAACAAACCCGATCACGTCACATCTCAACCTCTAGACGAAGGCTTTGGAGCAATCGTCAAAGCCTGCAAGAGCCTGAGAAGGCTTTCTCTCTCAGGTCTCCTTACAGACCAAGTCTTCCTCTACATCGGAATGTACGCGAATCAGCTCGAGATGCTCTCCATAGCCTTTGCAGGAGATACAGACAAAGGCATGCTATATGTGTTGAATGGTTGCAAAAAGATGAAGAAACTAGAGATAAGGGATAGTCCGTTTGGGGACACGGCGCTTCTTGCTGATGTGAGCAAGTATGAAACAATGCGATCCCTTTGGATGTCTTCATGTGAAGTCACACTCAGTGGATGCAAAAGGCTCGCAGAGAAAGCGCCATGGCTCAATGTAGAGATCATAAACGAGAATGATAATAACCGGATGGAAGAAAACGGACACGAGGGGAGGCAGAAAGTGGATAAGTTGTATCTGTACCGGACTGTGGTTGGGACAAGAATGGATGCGCCGCCATTTGTGTGGATTCTCTAA
Protein Sequence
- >AT3G26810.1
MNYFPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCLKSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRSFVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYENDPDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNTAVTEEGLVAISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRMEENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL