Gene Details:

• Gene ID: AT3G22200
• Gene Symbol: GABA-T, HER1, POP2
• Gene Name: GAMMA-AMINOBUTYRATE TRANSAMINASE, HEXENAL RESPONSE1, POLLEN-PISTIL INCOMPATIBILITY 2
• Description: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT3G22200.1     AT3G22200.2

Plant Ontology Annotations:

• PO:0020003  — plant ovule — óvulo vegetal (Spanish, exact), 胚珠 (Japanese, exact), Poaceae ovule (narrow), Zea ovule (narrow)
• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
• PO:0025195  — pollen tube cell — célula del tubo polínico (Spanish, exact), 花粉管細胞 (Japanese, exact)
• PO:0000293  — guard cell — célula guardiana (Spanish, exact), occlusive cell (exact), 孔辺細胞 (Japanese, exact)

Germplasm Phenotype:

• CS6385  — High accumulation of gamma-amino butyric acid (GABA) in flowers, causing a concentration-dependent defect in pollen tube growth, guidance and fertility; flowers on secondary inflorescences accumulate less GABA than those on primary branches and exhibit a corresponding increase in fertility; GABA concentrations increase along the pollen tube path, causing aberrant pollen tube growth and infertility; self-sterile, male gametophytic and female sporophytic defects.
• CS6385  — Hypersensitivity to NaCl and LiCl but not to mannitol in root growth assays. Under NaCl stress, pop2-1 mutant did not overaccumulate Na+ and Cl- but showed a slight decrease in K+ content. Additionally, mutant roots exhibited substantial metabolic changes upon salt stress, including overaccumulation of amino acids and decrease in carbohydrates content.
• CS6385  — Pollen tube growth defects in vivo. Primary root and dark-grown hypocotyl growth inhibition, at least in part due to cell elongation defects, upon exogenous GABA exposure.
• CS6386  — Increased accumulation of gamma-amino butyric acid (GABA) in flowers (but lower levels compared to pop2-1) causing a concentration-dependent defect in pollen tube growth, guidance and fertility; GABA concentrations increase along the pollen tube path, causing aberrant pollen tube growth and infertility; partially sterile.
• CS6387  — Increased accumulation of gamma-amino butyric acid (GABA) in flowers (but lower levels compared to pop2-1) causing a concentration-dependent defect in pollen tube growth, guidance and fertility; GABA concentrations increase along the pollen tube path, causing aberrant pollen tube growth and infertility; partially sterile.
• CS860071  — Primary root and dark-grown hypocotyl growth inhibition, at least in part due to cell elongation defects, upon exogenous GABA exposure.
• CS860071  — Reduced plant growth when grown on media containing gamma-aminobutyric acid (GABA) as the nitrogen source.
• her1-1  — The her1-1 mutant shows a specific resistance to E-2-hexenal-mediated root growth inhibition. her1-1 mutants have reduced apical dominance, but this phenotype is not observed in allelic pop2-1 (Ler) or pop2-3 (Ws) mutants indicating that this may be an ecotype-specific phenotype. her1-1 mutants produce fewer seeds and have higher levels of GABA than wild type plants. They are slightly more sensitive to E-2-pentenal and slightly less sensitive to E-2-heptenal than wild type seedlings in root elongation assays, but the reduced E-2-hexenal sensitivity is much more dramatic. her1 mutants, like wild type seedlings, produce higher GABA levels upon exposure to E-2-hexenal. The her1-1 mutation does not appear to affect the plants ability to degrade E-2-hexenal or to attach glutathione to it.

Function-related keywords:

• plant ovule ,  shoot axis apex ,  leaf lamina base ,  root ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen ,  pollen tube cell ,  pollen ,  guard cell

Literature:

• The impact of oxidative stress on Arabidopsis mitochondria.   DOI:  10.1046/j.1365-313x.2002.01474.x ;  PMID:  12492832
• Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.   DOI:  10.1105/tpc.016055 ;  PMID:  14671022
• Contribution of the GABA shunt to hypoxia-induced alanine accumulation in roots of Arabidopsis thaliana.   DOI:  10.1093/pcp/pcm171 ;  PMID:  18077464
• Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.   DOI:  10.1104/pp.108.126375 ;  PMID:  18775970
• Mutants of GABA transaminase (POP2) suppress the severe phenotype of succinic semialdehyde dehydrogenase (ssadh) mutants in Arabidopsis.   DOI:  10.1371/journal.pone.0003383 ;  PMID:  18846220
• Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function.   DOI:  10.1104/pp.109.147942 ;  PMID:  20018591
• The Arabidopsis pop2-1 mutant reveals the involvement of GABA transaminase in salt stress tolerance.   DOI:  10.1186/1471-2229-10-20 ;  PMID:  20122158
• γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots.   DOI:  10.1111/pce.12033 ;  PMID:  23148892
• WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis.   DOI:  10.1111/tpj.12953 ;  PMID:  26243404
• Gamma-aminobutyric acid depletion affects stomata closure and drought tolerance of Arabidopsis thaliana.   DOI:  10.1016/j.plantsci.2016.01.005 ;  PMID:  26940489
• Accumulation patterns of endogenous β-aminobutyric acid during plant development and defence in Arabidopsis thaliana.   DOI:  10.1111/plb.12940 ;  PMID:  30449064
• γ-Aminobutyric acid plays a key role in plant acclimation to a combination of high light and heat stress.   DOI:  10.1093/plphys/kiac010 ;  PMID:  35078231
• The impact of oxidative stress on Arabidopsis mitochondria.   DOI:  10.1046/j.1365-313x.2002.01474.x ;  PMID:  12492832
• Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.   DOI:  10.1105/tpc.016055 ;  PMID:  14671022
• The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses.   DOI:  10.1002/pmic.200500543 ;  PMID:  16502469
• A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture.   DOI:  10.1074/mcp.M600250-MCP200 ;  PMID:  17151019
• Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.   DOI:  10.1021/pr1009433 ;  PMID:  21166475
• Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling.   DOI:  10.1016/j.jprot.2017.06.004 ;  PMID:  28627464

Sequences: