Gene Details:
- Gene ID: AT3G19710
- Gene Symbol: BCAT4
- Gene Name: branched-chain aminotransferase4
- Description: branched-chain aminotransferase4;(source:Araport11)
- TAIR Accession: locus:2091216
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Gene Ontology:
- GO:0005829 — located in — cytosol
- GO:0009081 — involved in — branched-chain amino acid metabolic process
- GO:0009611 — involved in — response to wounding
- GO:0004084 — enables — branched-chain-amino-acid transaminase activity
- GO:0019761 — acts upstream of or within — glucosinolate biosynthetic process
- GO:0005737 — located in — cytoplasm
- GO:0009416 — involved in — response to light stimulus
- GO:0010326 — enables — methionine-oxo-acid transaminase activity
Germplasm Phenotype:
- SALK_013627 — The methionine-derived glucosinolate content was reduced to 53% in the leaves. Meanwhile, the level of free methionine was increased for about five-fold. Extremely high level of S-methylmethione, the main transport form of methionine, was found in the seeds.
- bcat3-1/bcat4-2/bcat6-1 — Smaller rosettes and inflorescences in comparison to wild type. In addition, there were dent-like cavities and chlorotic areas in the leaves. Moderately increased levels of Ala, Arg, His, Ile, Phe, Pro Thr, Trp and Tyr, and Leu. Extraordinary enhanced accumulation of Met. Significant decrease in levels of Met-derived glucosinolates and increase in Leu-derived glucosinolates.
- bcat4-2/bcat6-1 — Increased levels of Met, Ser, Val and S-methylmethionine (SMM) and decreased levels of Met-derived glucosinolates in leaves and seeds.
Literature:
- Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. DOI: 10.1242/jcs.00715 ; PMID: 12953064
- MINISEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucine-rich repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis. DOI: 10.1073/pnas.0508418102 ; PMID: 16293693
- Effect of sulfur availability on the integrity of amino acid biosynthesis in plants. DOI: 10.1007/s00726-005-0251-4 ; PMID: 16552493
- Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture. DOI: 10.1104/pp.106.081240 ; PMID: 16648215
- Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis. DOI: 10.1105/tpc.105.039339 ; PMID: 17056707
- Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. DOI: 10.1105/tpc.106.046458 ; PMID: 17114350
- An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach. DOI: 10.1105/tpc.106.044743 ; PMID: 17172353
- Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase–D-aspartate behavior during germination. DOI: 10.1111/j.1742-4658.2008.06279.x ; PMID: 18318836
- Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses. DOI: 10.1104/pp.108.115733 ; PMID: 18375600
- Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. DOI: 10.1104/pp.108.121038 ; PMID: 18650403
- Metabolic and transcriptomic changes induced in Arabidopsis by the rhizobacterium Pseudomonas fluorescens SS101. DOI: 10.1104/pp.112.207324 ; PMID: 23073694
- The cytosolic branched-chain aminotransferases of Arabidopsis thaliana influence methionine supply, salvage and glucosinolate metabolism. DOI: 10.1007/s11103-015-0312-3 ; PMID: 25851613
- The conserved transcription factors, MYB115 and MYB118, control expression of the newly evolved benzoyloxy glucosinolate pathway in Arabidopsis thaliana. DOI: 10.3389/fpls.2015.00343 ; PMID: 26029237
- The Ca(2+) Channel CNGC19 Regulates Arabidopsis Defense Against Spodoptera Herbivory. DOI: 10.1105/tpc.19.00057 ; PMID: 31076540
- Arabidopsis branched-chain aminotransferase 3 functions in both amino acid and glucosinolate biosynthesis. DOI: 10.1104/pp.107.111609 ; PMID: 18162591
Sequences:
cDNA Sequence
- >AT3G19710.1
AAAACAATTTGTTGTAAAAATAGAAAAAAAAAAAAAAAAAAAATTGTTGTAAACGTATATATAAAGAGAAGTATTCCAAAGCTTTATAGACAACTCAAGTTTCTTGCAATTACCTACTTACAACAAAGCAACTCGACTCGAACAAACACATCCATGGCTCCTTCTGCGCAACCTCTTCCTGTGAGTGTTTCGGATGAAAAATATGCGAATGTCAAGTGGGAAGAGTTGGCATTCAAGTTTGTTCGTACGGATTATATGTATGTTGCGAAGTGCAATCATGGAGAGAGTTTTCAAGAGGGGAAGATTCTTCCTTTTGCTGATTTGCAACTTAACCCTTGCGCTGCTGTTCTTCAGTATGGCCAGGGTTTATATGAAGGACTGAAAGCTTACAGGACAGAAGATGGTCGGATTCTGCTATTCCGACCAGACCAAAACGGTCTCCGCCTTCAAGCCGGAGCTGACAGACTCTATATGCCTTATCCTTCGGTCGATCAATTCGTCTCCGCCATCAAACAAGTTGCTCTTGCCAACAAGAAATGGATTCCTCCTCCGGGGAAAGGAACATTGTATATTAGGCCTATCTTGTTTGGGAGTGGTCCGATTCTTGGTTCATTTCCCATTCCTGAGACCACCTTCACAGCTTTTGCCTGTCCTGTTGGACGTTATCATAAGGATAACTCTGGTTTGAATCTGAAAATCGAAGATCAGTTTCGTCGAGCTTTTCCTAGTGGAACTGGTGGTGTGAAGAGCATCACAAACTATTGTCCTGTTTGGATACCATTGGCAGAGGCGAAAAAACAAGGTTTCTCTGATATTTTGTTTTTGGATGCTGCAACTGGCAAAAACATTGAAGAACTTTTCGCAGCTAATGTTTTTATGCTCAAGGGCAATGTTGTATCGACACCAACAATTGCAGGAACTATTTTGCCCGGAGTCACTCGAAACTGCGTAATGGAATTGTGTCGTGATTTCGGCTACCAGGTCGAGGAACGTACGATTCCTCTAGTGGACTTTCTCGATGCGGACGAAGCTTTCTGTACTGGCACTGCTTCCATTGTGACTAGTATTGCATCCGTAACCTTTAAAGACAAAAAGACCGGATTCAAAACAGGGGAAGAAACATTGGCTGCGAAGCTATACGAGACGTTAAGTGATATCCAGACGGGTCGGGTCGAGGATACCAAGGGATGGACGGTGGAGATTGACCGCCAGGGCTGAAAGTTGAAACTGTAACTTGATGCTAAATATGTGTGTGTGTGTATACAAAACTTATGTAAGAAACATCTGAAGATGTCTCTGATCTTTGTGATTGTGATGATCATGCATGTCCTATCGTTGCGATGTATTTTATAAATGTTCGTCTGTAAGTTATTTAATAACTATGGCTTTTTGCTTTATCTTTTTAATTATTTTTAAAGAGTATTATCATAGAAAATCTTATGGCTTTTCAA
CDS Sequence
- >AT3G19710.1
ATGGCTCCTTCTGCGCAACCTCTTCCTGTGAGTGTTTCGGATGAAAAATATGCGAATGTCAAGTGGGAAGAGTTGGCATTCAAGTTTGTTCGTACGGATTATATGTATGTTGCGAAGTGCAATCATGGAGAGAGTTTTCAAGAGGGGAAGATTCTTCCTTTTGCTGATTTGCAACTTAACCCTTGCGCTGCTGTTCTTCAGTATGGCCAGGGTTTATATGAAGGACTGAAAGCTTACAGGACAGAAGATGGTCGGATTCTGCTATTCCGACCAGACCAAAACGGTCTCCGCCTTCAAGCCGGAGCTGACAGACTCTATATGCCTTATCCTTCGGTCGATCAATTCGTCTCCGCCATCAAACAAGTTGCTCTTGCCAACAAGAAATGGATTCCTCCTCCGGGGAAAGGAACATTGTATATTAGGCCTATCTTGTTTGGGAGTGGTCCGATTCTTGGTTCATTTCCCATTCCTGAGACCACCTTCACAGCTTTTGCCTGTCCTGTTGGACGTTATCATAAGGATAACTCTGGTTTGAATCTGAAAATCGAAGATCAGTTTCGTCGAGCTTTTCCTAGTGGAACTGGTGGTGTGAAGAGCATCACAAACTATTGTCCTGTTTGGATACCATTGGCAGAGGCGAAAAAACAAGGTTTCTCTGATATTTTGTTTTTGGATGCTGCAACTGGCAAAAACATTGAAGAACTTTTCGCAGCTAATGTTTTTATGCTCAAGGGCAATGTTGTATCGACACCAACAATTGCAGGAACTATTTTGCCCGGAGTCACTCGAAACTGCGTAATGGAATTGTGTCGTGATTTCGGCTACCAGGTCGAGGAACGTACGATTCCTCTAGTGGACTTTCTCGATGCGGACGAAGCTTTCTGTACTGGCACTGCTTCCATTGTGACTAGTATTGCATCCGTAACCTTTAAAGACAAAAAGACCGGATTCAAAACAGGGGAAGAAACATTGGCTGCGAAGCTATACGAGACGTTAAGTGATATCCAGACGGGTCGGGTCGAGGATACCAAGGGATGGACGGTGGAGATTGACCGCCAGGGCTGA
Protein Sequence
- >AT3G19710.1
MAPSAQPLPVSVSDEKYANVKWEELAFKFVRTDYMYVAKCNHGESFQEGKILPFADLQLNPCAAVLQYGQGLYEGLKAYRTEDGRILLFRPDQNGLRLQAGADRLYMPYPSVDQFVSAIKQVALANKKWIPPPGKGTLYIRPILFGSGPILGSFPIPETTFTAFACPVGRYHKDNSGLNLKIEDQFRRAFPSGTGGVKSITNYCPVWIPLAEAKKQGFSDILFLDAATGKNIEELFAANVFMLKGNVVSTPTIAGTILPGVTRNCVMELCRDFGYQVEERTIPLVDFLDADEAFCTGTASIVTSIASVTFKDKKTGFKTGEETLAAKLYETLSDIQTGRVEDTKGWTVEIDRQG