Gene Details:
- Gene ID: AT3G15170
- Gene Symbol: ANAC054, ATNAC1, CUC1
- Gene Name: Arabidopsis NAC domain containing protein 54, CUP-SHAPED COTYLEDON1
- Description: NAC (No Apical Meristem) domain transcriptional regulator superfamily protein;(source:Araport11)
- TAIR Accession: locus:2083656
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0000003 — whole plant — planta entera (Spanish, exact), 植物体全体 (Japanese, exact), clonal colony (related), colony (related), bush (narrow), frutex (narrow), frutices (narrow), gametophyte (narrow), herb (narrow), liana (narrow), prothalli (narrow), prothallium (narrow), prothallus (narrow), seedling (narrow), shrub (narrow), sporophyte (narrow), suffrutex (narrow), suffrutices (narrow), tree (narrow), vine (narrow), woody clump (narrow), genet (broad), ramet (broad)
Gene Ontology:
- GO:0010223 — acts upstream of or within — secondary shoot formation
- GO:0009793 — acts upstream of or within — embryo development ending in seed dormancy
- GO:0003700 — enables — DNA-binding transcription factor activity
- GO:0010072 — acts upstream of or within — primary shoot apical meristem specification
- GO:0048527 — acts upstream of or within — lateral root development
- GO:0048467 — acts upstream of or within — gynoecium development
- GO:0005634 — located in — nucleus
- GO:0000976 — enables — transcription cis-regulatory region binding
- GO:0006355 — involved in — regulation of DNA-templated transcription
- GO:0090691 — acts upstream of or within — formation of plant organ boundary
- GO:0009908 — acts upstream of or within — flower development
- GO:0010014 — acts upstream of or within — meristem initiation
- GO:0051782 — acts upstream of or within — negative regulation of cell division
Germplasm Phenotype:
- CS3869 — semi-dominant; cotyledons fuse along one side at very low frequency (approx 0.5 %), slight fusion of sepals, stamen fusion at very low frequency.
- CS3871 — semi-dominant; phenotype of segregating descendants is the following: a) cuc1-1 cuc2-1 exhibits cotyledons fused along both sides to form a single cup-shaped cotyledon, shoot apical meristem is missing and no shoots are produced under normal conditions, seedling lethal, shoots can be induced from calli and produce flowers with severly fused sepals and stamens and with reduced petals, septa and ovules; b) cuc1-1 cuc2-1 /+ produce shoots with more severly fused sepals than cuc1-1 single mutant plants, facilitating identification of heterozygous adult plants; c) cuc1-1 produce shoots with sepals slightly fused.
- cuc1-1 cuc3-101 — High frequency production of cup-shaped cotyledons in seedlings, most form a primary shoot.
- cuc1-1 cuc3-101 — Production of the first pair of leaf primordia is significantly delayed compared to the wild type. This likely reflects a defect in embryonic shoot meristem initiation.
- cuc1-1 syd-2 — Strongly enhanced cotyledon fusion phenotype of cuc1-1.
Function-related keywords:
Literature:
- Shoot apical meristem and cotyledon formation during Arabidopsis embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS genes. DOI: 10.1242/dev.126.8.1563 ; PMID: 10079219
- Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. DOI: 10.1105/tpc.9.6.841 ; PMID: 9212461
- Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. DOI: 10.1126/science.290.5499.2105 ; PMID: 11118137
- The CUP-SHAPED COTYLEDON1 gene of Arabidopsis regulates shoot apical meristem formation. DOI: 10.1242/dev.128.7.1127 ; PMID: 11245578
- Roles of PIN-FORMED1 and MONOPTEROS in pattern formation of the apical region of the Arabidopsis embryo. DOI: 10.1242/dev.129.17.3965 ; PMID: 12163400
- Developmental events and shoot apical meristem gene expression patterns during shoot development in Arabidopsis thaliana. DOI: 10.1046/j.1365-313x.2002.01479.x ; PMID: 12492830
- The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on calli. DOI: 10.1093/pcp/pcg038 ; PMID: 12610213
- Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. DOI: 10.1046/j.1365-313x.2003.01759.x ; PMID: 12787253
- The CUP-SHAPED COTYLEDON3 gene is required for boundary and shoot meristem formation in Arabidopsis. DOI: 10.1105/tpc.012203 ; PMID: 12837947
- CUC1 gene activates the expression of SAM-related genes to induce adventitious shoot formation. DOI: 10.1046/j.1365-313x.2003.01911.x ; PMID: 14617069
- Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DOI: 10.1093/dnares/10.6.239 ; PMID: 15029955
- MicroRNA regulation of NAC-domain targets is required for proper formation and separation of adjacent embryonic, vegetative, and floral organs. DOI: 10.1016/j.cub.2004.06.022 ; PMID: 15202996
- PETAL LOSS, a trihelix transcription factor gene, regulates perianth architecture in the Arabidopsis flower. DOI: 10.1242/dev.01279 ; PMID: 15269176
- MicroRNA regulation of the CUC genes is required for boundary size control in Arabidopsis meristems. DOI: 10.1242/dev.01320 ; PMID: 15294871
- PIN-FORMED1 and PINOID regulate boundary formation and cotyledon development in Arabidopsis embryogenesis. DOI: 10.1242/dev.01388 ; PMID: 15371311
- The GURKE gene encoding an acetyl-CoA carboxylase is required for partitioning the embryo apex into three subregions in Arabidopsis. DOI: 10.1093/pcp/pch148 ; PMID: 15509834
- AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. DOI: 10.1111/j.1365-313X.2005.02575.x ; PMID: 16359384
- KNAT6: an Arabidopsis homeobox gene involved in meristem activity and organ separation. DOI: 10.1105/tpc.106.041988 ; PMID: 16798887
- A role for chromatin remodeling in regulation of CUC gene expression in the Arabidopsis cotyledon boundary. DOI: 10.1242/dev.02508 ; PMID: 16854978
- The ENHANCER OF SHOOT REGENERATION 2 gene in Arabidopsis regulates CUP-SHAPED COTYLEDON 1 at the transcriptional level and controls cotyledon development. DOI: 10.1093/pcp/pcl023 ; PMID: 17056621
- Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation. DOI: 10.1105/tpc.106.045716 ; PMID: 17122068
- The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis. DOI: 10.1105/tpc.106.045617 ; PMID: 17098808
- TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis. DOI: 10.1105/tpc.106.044792 ; PMID: 17307931
- Arabidopsis genes AS1, AS2, and JAG negatively regulate boundary-specifying genes to promote sepal and petal development. DOI: 10.1104/pp.107.113787 ; PMID: 18156293
- Interplay of miR164, CUP-SHAPED COTYLEDON genes and LATERAL SUPPRESSOR controls axillary meristem formation in Arabidopsis thaliana. DOI: 10.1111/j.1365-313X.2008.03483.x ; PMID: 18346190
- Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. DOI: 10.1111/j.1365-313X.2008.03715.x ; PMID: 18980654
- Identification of novel meristem factors involved in shoot regeneration through the analysis of temperature-sensitive mutants of Arabidopsis. DOI: 10.1111/j.1365-313X.2008.03750.x ; PMID: 19054368
- The role of ARGONAUTE1 (AGO1) in meristem formation and identity. DOI: 10.1016/j.ydbio.2005.01.031 ; PMID: 15882589
- Carpel development in a floral mutant of dioecious Silene latifolia producing asexual and female-like flowers. DOI: 10.1016/j.jplph.2009.04.004 ; PMID: 19457581
- Sex determination in plants. DOI: 10.4161/psb.2.3.3728 ; PMID: 19704689
- Two separate pathways including SlCLV1, SlSTM and SlCUC that control carpel development in a bisexual mutant of Silene latifolia. DOI: 10.1093/pcp/pcp187 ; PMID: 20064843
- Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development. DOI: 10.1105/tpc.110.081448 ; PMID: 21258003
- CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells. DOI: 10.1111/j.1365-313X.2011.04571.x ; PMID: 21435050
- A mechanistic link between STM and CUC1 during Arabidopsis development. DOI: 10.1104/pp.111.177709 ; PMID: 21685178
- The class I protein AtTCP15 modulates plant development through a pathway that overlaps with the one affected by CIN-like TCP proteins. DOI: 10.1093/jxb/err305 ; PMID: 22016421
- WOX2 and STIMPY-LIKE/WOX8 promote cotyledon boundary formation in Arabidopsis. DOI: 10.1111/j.1365-313X.2012.05113.x ; PMID: 22827849
- A set of Arabidopsis thaliana miRNAs involve shoot regeneration in vitro. DOI: 10.4161/psb.23479 ; PMID: 23333958
- Redox modulation of plant developmental regulators from the class I TCP transcription factor family. DOI: 10.1104/pp.113.216416 ; PMID: 23686421
- An integrative model of the control of ovule primordia formation. DOI: 10.1111/tpj.12309 ; PMID: 23941199
- The CUC1 and CUC2 genes promote carpel margin meristem formation during Arabidopsis gynoecium development. DOI: 10.3389/fpls.2014.00165 ; PMID: 24817871
- Genetic interaction between GROWTH-REGULATING FACTOR and CUP-SHAPED COTYLEDON in organ separation. DOI: 10.4161/15592324.2014.988071 ; PMID: 25761011
- A conserved role for CUP-SHAPED COTYLEDON genes during ovule development. DOI: 10.1111/tpj.12923 ; PMID: 26119568
- SHOOT MERISTEMLESS trafficking controls axillary meristem formation, meristem size and organ boundaries in Arabidopsis. DOI: 10.1111/tpj.13504 ; PMID: 28161901
- Roles of miR319 and TCP Transcription Factors in Leaf Development. DOI: 10.1104/pp.17.00732 ; PMID: 28842549
- A transcriptomics approach uncovers novel roles for poly(ADP-ribosyl)ation in the basal defense response in Arabidopsis thaliana. DOI: 10.1371/journal.pone.0190268 ; PMID: 29284022
- Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network. DOI: 10.1242/dev.157081 ; PMID: 29650590
- The plant hormone auxin directs timing of xylem development by inhibition of secondary cell wall deposition through repression of secondary wall NAC-domain transcription factors. DOI: 10.1111/ppl.12766 ; PMID: 29808599
- CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 regulate cytokinin homeostasis to determine ovule number in Arabidopsis. DOI: 10.1093/jxb/ery281 ; PMID: 30312436
- Nonsense-Mediated mRNA Decay Deficiency Affects the Auxin Response and Shoot Regeneration in Arabidopsis. DOI: 10.1093/pcp/pcz154 ; PMID: 31386149
- Transcriptional regulation of nitrogen-associated metabolism and growth. DOI: 10.1038/s41586-018-0656-3 ; PMID: 30356219
- A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis. DOI: 10.1105/tpc.19.00562 ; PMID: 31806676
- Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised Arabidopsis Roots. DOI: 10.3390/plants9101378 ; PMID: 33081176
- Warm Temperature Promotes Shoot Regeneration in Arabidopsis thaliana. DOI: 10.1093/pcp/pcac017 ; PMID: 35157760
- Expression of the auxin biosynthetic genes YUCCA1 and YUCCA4 is dependent on the boundary regulators CUP-SHAPED COTYLEDON genes in the Arabidopsis thaliana embryo. DOI: 10.5511/plantbiotechnology.21.0924a ; PMID: 35800963
- and noncell-autonomous AUXIN RESPONSE FACTOR3 controls meristem proliferation and phyllotactic patterns. DOI: 10.1093/plphys/kiac370 ; PMID: 35972411
- De novo stem cell establishment in meristems requires repression of organ boundary cell fate. DOI: 10.1093/plcell/koac269 ; PMID: 36029254
- Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. DOI: 10.1126/science.290.5499.2105 ; PMID: 11118137
- Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. DOI: 10.1093/molbev/msj051 ; PMID: 16280546
Sequences:
cDNA Sequence
- >AT3G15170.1
AACAGGAGAAACACAGATCATTAAAATAAAGGACCTTTTTTATTTTCCTCTTCTTTTCTCTTCCTTTATCTTTCAACATTTGGGAACTCTAGGGTTTTGTTATTTTTAGAGCTTTCTTCTTGTGCCGACAATGGATGTTGATGTGTTTAACGGTTGGGGGAGGCCAAGATTTGAAGATGAATCCCTTATGCCACCTGGGTTTAGGTTTCATCCAACTGATGAAGAGCTGATCACTTACTATCTCCTCAAGAAGGTTCTTGACTCTAATTTCTCTTGTGCCGCCATTTCTCAAGTTGATCTCAACAAGTCTGAGCCTTGGGAGCTTCCTGAGAAAGCGAAAATGGGGGAGAAGGAGTGGTACTTCTTCACACTAAGAGACCGTAAATACCCAACGGGACTGAGAACGAACAGAGCAACAGAAGCTGGTTACTGGAAAGCCACTGGTAAAGACAGAGAGATCAAAAGCTCAAAGACAAAATCACTTCTCGGGATGAAGAAAACTCTTGTCTTTTACAAAGGCAGAGCTCCTAAAGGAGAGAAGAGTTGTTGGGTCATGCATGAGTATCGCCTTGACGGCAAATTCTCTTACCATTACATTTCCTCCTCCGCTAAGGATGAATGGGTTCTCTGTAAAGTTTGTCTGAAAAGCGGCGTAGTTAGTAGAGAGACGAACTTGATCTCTTCTTCTTCTTCTTCTGCCGTCACCGGAGAGTTCTCCTCTGCCGGTTCTGCAATTGCTCCGATCATCAATACCTTTGCGACGGAGCACGTGTCCTGTTTCTCCAATAACTCTGCTGCTCATACCGATGCGAGCTTTCATACATTCCTTCCCGCTCCACCGCCGTCACTGCCCCCACGTCAGCCACGTCACGTCGGTGATGGCGTGGCGTTTGGTCAGTTTCTGGATTTGGGATCATCGGGACAGATTGATTTCGATGCAGCAGCAGCAGCGTTCTTTCCGAATCTACCTTCTCTGCCTCCCACGGTTCTTCCTCCTCCTCCGTCATTTGCAATGTACGGTGGAGGCTCCCCCGCCGTGAGTGTGTGGCCGTTTACTCTCTGATCGCCATGCGGTCATTAAACTATCTTTACTATCTTTTAATCTAAGAGAAAAACTACCTTGGGTTGCTTAATATTAGTGGCTTTGTATTAGTCTCGAATCATTTCCTCGTAGCATTTTGCTGTTGTACGTGTTTGTAGTAACGTCTTTGTGCATGTTTTTAAACATGAACCTGC
CDS Sequence
- >AT3G15170.1
ATGGATGTTGATGTGTTTAACGGTTGGGGGAGGCCAAGATTTGAAGATGAATCCCTTATGCCACCTGGGTTTAGGTTTCATCCAACTGATGAAGAGCTGATCACTTACTATCTCCTCAAGAAGGTTCTTGACTCTAATTTCTCTTGTGCCGCCATTTCTCAAGTTGATCTCAACAAGTCTGAGCCTTGGGAGCTTCCTGAGAAAGCGAAAATGGGGGAGAAGGAGTGGTACTTCTTCACACTAAGAGACCGTAAATACCCAACGGGACTGAGAACGAACAGAGCAACAGAAGCTGGTTACTGGAAAGCCACTGGTAAAGACAGAGAGATCAAAAGCTCAAAGACAAAATCACTTCTCGGGATGAAGAAAACTCTTGTCTTTTACAAAGGCAGAGCTCCTAAAGGAGAGAAGAGTTGTTGGGTCATGCATGAGTATCGCCTTGACGGCAAATTCTCTTACCATTACATTTCCTCCTCCGCTAAGGATGAATGGGTTCTCTGTAAAGTTTGTCTGAAAAGCGGCGTAGTTAGTAGAGAGACGAACTTGATCTCTTCTTCTTCTTCTTCTGCCGTCACCGGAGAGTTCTCCTCTGCCGGTTCTGCAATTGCTCCGATCATCAATACCTTTGCGACGGAGCACGTGTCCTGTTTCTCCAATAACTCTGCTGCTCATACCGATGCGAGCTTTCATACATTCCTTCCCGCTCCACCGCCGTCACTGCCCCCACGTCAGCCACGTCACGTCGGTGATGGCGTGGCGTTTGGTCAGTTTCTGGATTTGGGATCATCGGGACAGATTGATTTCGATGCAGCAGCAGCAGCGTTCTTTCCGAATCTACCTTCTCTGCCTCCCACGGTTCTTCCTCCTCCTCCGTCATTTGCAATGTACGGTGGAGGCTCCCCCGCCGTGAGTGTGTGGCCGTTTACTCTCTGA
Protein Sequence
- >AT3G15170.1
MDVDVFNGWGRPRFEDESLMPPGFRFHPTDEELITYYLLKKVLDSNFSCAAISQVDLNKSEPWELPEKAKMGEKEWYFFTLRDRKYPTGLRTNRATEAGYWKATGKDREIKSSKTKSLLGMKKTLVFYKGRAPKGEKSCWVMHEYRLDGKFSYHYISSSAKDEWVLCKVCLKSGVVSRETNLISSSSSSAVTGEFSSAGSAIAPIINTFATEHVSCFSNNSAAHTDASFHTFLPAPPPSLPPRQPRHVGDGVAFGQFLDLGSSGQIDFDAAAAAFFPNLPSLPPTVLPPPPSFAMYGGGSPAVSVWPFTL