Gene Details:

• Gene ID: AT3G12120
• Gene Symbol: AtFAD2, FAD2
• Gene Name: fatty acid desaturase 2
• Description: fatty acid desaturase 2;(source:Araport11)
• TAIR Accession: locus:2099297
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT3G12120.1     AT3G12120.2

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)

Gene Ontology:

• GO:0016720  — enables — delta12-fatty acid dehydrogenase activity
• GO:1901362  — acts upstream of or within — organic cyclic compound biosynthetic process
• GO:0005783  — located in — endoplasmic reticulum
• GO:0005634  — located in — nucleus
• GO:0006636  — involved in — unsaturated fatty acid biosynthetic process
• GO:0102985  — enables — delta12-fatty-acid desaturase activity
• GO:0009414  — acts upstream of or within — response to water deprivation
• GO:0045485  — enables — omega-6 fatty acid desaturase activity
• GO:0009416  — acts upstream of or within — response to light stimulus
• GO:0008299  — acts upstream of or within — isoprenoid biosynthetic process
• GO:0044283  — acts upstream of or within — small molecule biosynthetic process
• GO:0006082  — acts upstream of or within — organic acid metabolic process
• GO:0050184  — enables — phosphatidylcholine desaturase activity
• GO:0009606  — acts upstream of or within — tropism

Germplasm Phenotype:

• CS68918  — Biochemical phenotypes of fad2 vte1-1 include: deficiency in ER fatty acid desaturation and also complete loss of all tocochromanols. Relative to the vte2-1 mutation, vte1-1 single mutant exhibits intermediate phenotypes under low temperatures including increased anthocyanin levels (purple leaf coloration), reduced growth and seed production. These vte1-1 single mutant phenotypes are fully suppressed by combining the vte1-1 and fad2 mutations in the double mutant.
• CS68919  — Biochemical phenotypes of fad2 vte2-1 include: deficiency in ER fatty acid desaturation and lack of all tocopherols and pathway intermediates. vte2-1 single mutant exhibits the following phenotypes under low temperatures: increased anthocyanin levels (purple leaf coloration), reduced growth and seed production. These vte2-1 single mutant phenotypes are fully suppressed by combining the vte2-1 and fad2 mutations in the double mutant.
• CS8041  — deficient in 18:1 fatty acid desaturase; increase in 18:1 fatty acids, decrease in 18:2 and 18:3 fatty acids, chloroplast membrane components; no visible phenotypic effects.

Function-related keywords:

• shoot axis apex ,  leaf lamina base ,  root ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen

Literature:

• Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis.   DOI:  10.1073/pnas.94.4.1142 ;  PMID:  9037020
• The impact of alteration of polyunsaturated fatty acid levels on C6-aldehyde formation of Arabidopsis thaliana leaves.   DOI:  10.1104/pp.111.3.805 ;  PMID:  8754681
• Digalactosyldiacylglycerol synthesis in chloroplasts of the Arabidopsis dgd1 mutant.   DOI:  10.1104/pp.010780 ;  PMID:  11891245
• Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana.   DOI:  10.1007/s00425-003-1015-6 ;  PMID:  14520576
• and jasmonic acid-mediated defense pathways in the Arabidopsis ssi2 mutant.   DOI:  10.1105/tpc.017301 ;  PMID:  14615603
• Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini.   DOI:  10.1111/j.1365-313x.2004.01949.x ;  PMID:  14690501
• Role of salicylic acid and fatty acid desaturation pathways in ssi2-mediated signaling.   DOI:  10.1104/pp.105.071662 ;  PMID:  16306139
• Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells.   DOI:  10.1104/pp.106.091496 ;  PMID:  17114270
• The SebHLH transcription factor mediates trans-activation of the SeFAD2 gene and G-box elements.   DOI:  10.1007/s11103-007-9165-8 ;  PMID:  17420955
• Alternative oxidase involvement in cold stress response of Arabidopsis thaliana fad2 and FAD3+ cell suspensions altered in membrane lipid composition.   DOI:  10.1093/pcp/pcm061 ;  PMID:  17507388
• An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library.   DOI:  10.1186/1471-2229-7-42 ;  PMID:  17672910
• Tocopherols modulate extraplastidic polyunsaturated fatty acid metabolism in Arabidopsis at low temperature.   DOI:  10.1105/tpc.107.054718 ;  PMID:  18314499
• Artificial trans-acting siRNAs confer consistent and effective gene silencing.   DOI:  10.1104/pp.108.118307 ;  PMID:  18441221
• Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.   DOI:  10.1104/pp.108.121038 ;  PMID:  18650403
• Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.   DOI:  10.1104/pp.108.126375 ;  PMID:  18775970
• Genetically modified Arabidopsis thaliana cells reveal the involvement of the mitochondrial fatty acid composition in membrane basal and uncoupling protein-mediated proton leaks.   DOI:  10.1093/pcp/pcp144 ;  PMID:  19875678
• Mutations of the ER to plastid lipid transporters TGD1, 2, 3 and 4 and the ER oleate desaturase FAD2 suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.   DOI:  10.1111/j.1365-313X.2010.04212.x ;  PMID:  20345604
• Lipid transport mediated by Arabidopsis TGD proteins is unidirectional from the endoplasmic reticulum to the plastid.   DOI:  10.1093/pcp/pcq053 ;  PMID:  20410050
• Towards the genetic architecture of seed lipid biosynthesis and accumulation in Arabidopsis thaliana.   DOI:  10.1038/hdy.2011.54 ;  PMID:  21731053
• Microarray analysis of differentially expressed genes between Brassica napus and low-oleic acid contents.   DOI:  10.1007/s00299-011-1213-9 ;  PMID:  22203212
• Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.   DOI:  10.1186/1471-2229-12-4 ;  PMID:  22233193
• In silico identification and comparative genomics of candidate genes involved in biosynthesis and accumulation of seed oil in plants.   DOI:  10.1155/2012/914843 ;  PMID:  22312320
• Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation.   DOI:  10.1371/journal.pone.0031370 ;  PMID:  22384013
• Modification of Seed Oil Composition in Arabidopsis by Artificial microRNA-Mediated Gene Silencing.   DOI:  10.3389/fpls.2012.00168 ;  PMID:  22866055
• Acyl-lipid metabolism.   DOI:  10.1199/tab.0161 ;  PMID:  23505340
• Mutants of Arabidopsis with alterations in seed lipid fatty acid composition.   DOI:  10.1007/BF00224392 ;  PMID:  24220901
• TRANSPARENT TESTA2 regulates embryonic fatty acid biosynthesis by targeting FUSCA3 during the early developmental stage of Arabidopsis seeds.   DOI:  10.1111/tpj.12426 ;  PMID:  24397827
• FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channeling in vivo.   DOI:  10.1074/jbc.M114.572883 ;  PMID:  24811169
• Combined transgenic expression of Punica granatum conjugase (FADX) and FAD2 desaturase in high linoleic acid Arabidopsis thaliana mutant leads to increased accumulation of punicic acid.   DOI:  10.1007/s00425-014-2109-z ;  PMID:  25000918
• The toc132toc120 heterozygote mutant of Arabidopsis thaliana accumulates reduced levels of hexadecatrienoic acid.   DOI:  10.1016/j.plaphy.2015.09.006 ;  PMID:  26381195
• Levels of polyunsaturated fatty acids correlate with growth rate in plant cell cultures.   DOI:  10.1038/srep15207 ;  PMID:  26469123
• ALA10, a Phospholipid Flippase, Controls FAD2/FAD3 Desaturation of Phosphatidylcholine in the ER and Affects Chloroplast Lipid Composition in Arabidopsis thaliana.   DOI:  10.1104/pp.15.01557 ;  PMID:  26620528
• Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants.   DOI:  10.1016/j.molp.2015.12.010 ;  PMID:  26712506
• Genome-Wide Association Study in Arabidopsis thaliana of Natural Variation in Seed Oil Melting Point: A Widespread Adaptive Trait in Plants.   DOI:  10.1093/jhered/esw008 ;  PMID:  26865732
• Genome Wide Analysis of Fatty Acid Desaturation and Its Response to Temperature.   DOI:  10.1104/pp.16.01907 ;  PMID:  28108698
• Plant surface metabolites as potent antifungal agents.   DOI:  10.1016/j.plaphy.2020.02.026 ;  PMID:  32112998
• PUB11-Dependent Ubiquitination of the Phospholipid Flippase ALA10 Modifies ALA10 Localization and Affects the Pool of Linolenic Phosphatidylcholine.   DOI:  10.3389/fpls.2020.01070 ;  PMID:  32760418
• Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21.   DOI:  10.1111/tpj.15540 ;  PMID:  34643970
• The seed-specific transcription factor DPBF2 modulates the fatty acid composition in seeds.   DOI:  10.1002/pld3.395 ;  PMID:  35388372

Sequences: