Gene Details:
- Gene ID: AT3G08560
- Gene Symbol: VHA-E2
- Gene Name: vacuolar H+-ATPase subunit E isoform 2
- Description: vacuolar H+-ATPase subunit E isoform 2;(source:Araport11)
- TAIR Accession: locus:2077893
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Plant Ontology Annotations:
- PO:0020099 — microgametophyte vegetative cell — célula vegetativa (Spanish, exact), 栄養細胞 (Japanese, exact), tube cell (related)
- PO:0025281 — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
Gene Ontology:
- GO:0005634 — located in — nucleus
- GO:0005773 — located in — vacuole
- GO:0046961 — enables — proton-transporting ATPase activity, rotational mechanism
- GO:0005829 — located in — cytosol
- GO:0033178 — part of — proton-transporting two-sector ATPase complex, catalytic domain
Function-related keywords:
Literature:
- A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase. DOI: 10.1016/s1360-1385(02)02240-9 ; PMID: 11950611
- Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. DOI: 10.1093/pcp/pch099 ; PMID: 15215502
- Arabidopsis vacuolar H-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis. DOI: 10.1111/j.1365-313X.2004.02283.x ; PMID: 15610355
- Green sperm. Identification of male gamete promoters in Arabidopsis. DOI: 10.1104/pp.104.054213 ; PMID: 16055690
- Proteome mapping of mature pollen of Arabidopsis thaliana. DOI: 10.1002/pmic.200402011 ; PMID: 16247729
- Synteny conservation between the Prunus genome and both the present and ancestral Arabidopsis genomes. DOI: 10.1186/1471-2164-7-81 ; PMID: 16615871
- Integrating membrane transport with male gametophyte development and function through transcriptomics. DOI: 10.1104/pp.105.074708 ; PMID: 16607029
- Comparative transcriptomics of Arabidopsis sperm cells. DOI: 10.1104/pp.108.125229 ; PMID: 18667720
- Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms. DOI: 10.1016/j.ejcb.2009.11.008 ; PMID: 19945769
- A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase. DOI: 10.1016/s1360-1385(02)02240-9 ; PMID: 11950611
- Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. DOI: 10.1093/pcp/pch099 ; PMID: 15215502
- Proteome mapping of mature pollen of Arabidopsis thaliana. DOI: 10.1002/pmic.200402011 ; PMID: 16247729
- Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis. DOI: 10.1186/1471-2121-9-28 ; PMID: 18507826
Sequences:
cDNA Sequence
- >AT3G08560.1
GATGTGATGATTGTTGTCTGAGAGAGAGAGAAGAAGAAGATGAACGATGCGGATGTGTCAAAGCAGATACAGCAGATGGTCCGGTTCATCCGGCAAGAAGCAGAAGAGAAAGCAAACGAGATCTCCATCTCCGCCGAGGAGGAATTCAACATAGAAAGACTTCAGCTTCTCGAGTCTGCCAAGCGTAAGCTCCGTCAAGACTATGACCGCAAGCTCAAGCAGGTTGATATCCGCAAGCGAATCGACTACTCCACACAACTGAATGCAAGTAGGATTAAGTACCTTCAAGCTCAAGATGACGTTGTCACTGCCATGAAAGACTCCGCAGCTAAGGATCTTCTCCGTGTCTCCAACGACAAGAACAATTACAAGAAGCTTCTTAAGAGTCTCATCATTGAGAGTTTGCTGCGCCTGAAAGAGCCATCAGTGCTGCTGAGATGCAGAGAAATGGACAAGAAGGTTGTTGAATCGGTCATTGAAGATGCTAAAAGACAGTACGCGGAAAAAGCCAAAGTCGGGTCTCCTAAGATCACCATCGACGAAAAGGTCTTCCTCCCTCCACCTCCTAATCCGAAGCTCCCTGATTCCCACGACCCTCACTGCTCGGGTGGGGTGGTGCTGGCCTCTCAAGACGGCAAGATTGTCTGCGAGAACACTTTAGACGCACGCCTTGACGTCGCCTTCAGACAGAAGCTTCCCCAGATCCGGACGCGCCTCGTTGGAGCTCCTGAAACCTCCAGAGCATGA
CDS Sequence
- >AT3G08560.1
ATGAACGATGCGGATGTGTCAAAGCAGATACAGCAGATGGTCCGGTTCATCCGGCAAGAAGCAGAAGAGAAAGCAAACGAGATCTCCATCTCCGCCGAGGAGGAATTCAACATAGAAAGACTTCAGCTTCTCGAGTCTGCCAAGCGTAAGCTCCGTCAAGACTATGACCGCAAGCTCAAGCAGGTTGATATCCGCAAGCGAATCGACTACTCCACACAACTGAATGCAAGTAGGATTAAGTACCTTCAAGCTCAAGATGACGTTGTCACTGCCATGAAAGACTCCGCAGCTAAGGATCTTCTCCGTGTCTCCAACGACAAGAACAATTACAAGAAGCTTCTTAAGAGTCTCATCATTGAGAGTTTGCTGCGCCTGAAAGAGCCATCAGTGCTGCTGAGATGCAGAGAAATGGACAAGAAGGTTGTTGAATCGGTCATTGAAGATGCTAAAAGACAGTACGCGGAAAAAGCCAAAGTCGGGTCTCCTAAGATCACCATCGACGAAAAGGTCTTCCTCCCTCCACCTCCTAATCCGAAGCTCCCTGATTCCCACGACCCTCACTGCTCGGGTGGGGTGGTGCTGGCCTCTCAAGACGGCAAGATTGTCTGCGAGAACACTTTAGACGCACGCCTTGACGTCGCCTTCAGACAGAAGCTTCCCCAGATCCGGACGCGCCTCGTTGGAGCTCCTGAAACCTCCAGAGCATGA
Protein Sequence
- >AT3G08560.1
MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRKLKQVDIRKRIDYSTQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNNYKKLLKSLIIESLLRLKEPSVLLRCREMDKKVVESVIEDAKRQYAEKAKVGSPKITIDEKVFLPPPPNPKLPDSHDPHCSGGVVLASQDGKIVCENTLDARLDVAFRQKLPQIRTRLVGAPETSRA