Gene Details:
- Gene ID: AT3G04520
- Gene Symbol: THA2
- Gene Name: threonine aldolase 2
- Description: threonine aldolase 2;(source:Araport11)
- TAIR Accession: locus:2100860
- Genome: Araport11_genome_release
- Species: Arabidopsis thaliana
Transcripts:
Gene Ontology:
- GO:0005634 — located in — nucleus
- GO:0016829 — enables — lyase activity
- GO:0006520 — involved in — amino acid metabolic process
- GO:0004793 — enables — threonine aldolase activity
- GO:0006567 — involved in — threonine catabolic process
- GO:0005829 — is active in — cytosol
- GO:0006567 — acts upstream of or within — threonine catabolic process
- GO:0006545 — involved in — glycine biosynthetic process
- GO:0005829 — located in — cytosol
- GO:0008732 — enables — L-allo-threonine aldolase activity
Germplasm Phenotype:
- CS6934 — 25% of seeds in developing silique of heterozygous plants are albino and lethal; mature mutant and wild type seeds look identical; but 25% of the developing seedlings are albino, fail to mature beyond the cotyledon stage and do not accumulate any chlorophyll pigments. Mutant growth not rescued with exogenous addition of metabolically related amino acids. Significant alteration in the amino acid profiles of most plant tissue types, increased seed Threonine (Thr) content; reduced Glicyn (a product of Thr aldolase reaction) content in all plant tissues; increased branched-chain amino acids (Ile, Leu and Val) in vegetative tissues, except in stems, where there is a decrease. Reduced levels of organic acids and sugars, citrate and isocitrate levels in seeds and flowers are significantly lower than in wild type; levels of most sugars are significantly decreased in flowers and siliques.
- CS6935 — Seeds, seedlings and whole homozygous plants do not show any visible phenotypic differences compared to wild type; not lethal. Analysis of the seed amino acid content show no significant Threonine (Thr) increase compared to wild type; Thr and Ile content in plants is not significantly different from the wild type; reduced levels of organic acids and sugars, citrate and isocitrate levels in seeds and flowers are significantly lower than in wild type.
- CS6960 — 25% of seeds in developing silique of heterozygous plants are albino and lethal; mature mutant and wild type seeds look identical; but 25% of the developing seedlings are albino, fail to mature beyond the cotyledon stage and do not accumulate any chlorophyll pigments. Mutant growth not rescued with exogenous addition of metabolically related amino acids. Significant alteration in the amino acid profiles of most plant tissue types, increased seed Threonine (Thr) content; reduced Glicyn (a product of Thr aldolase reaction) content in all plant tissues; increased branched-chain amino acids (Ile, Leu and Val) in vegetative tissues, except in stems, where there is a decrease. Reduced levels of organic acids and sugars, citrate and isocitrate levels in seeds and flowers are significantly lower than in wild type; levels of most sugars are significantly decreased in flowers and siliques.
- SAIL_5-CO1 — albino developing seeds. Heterozygous seeds look normal but have moderate increases of threonine and branched-chain amino acids.
- SALK_013854 — homozygote plant has no visible phenotype. No significant seed threonine increase either.
- SALK_129163 — albino developing seeds. Heterozygous seeds look normal but have moderate increases of threonine and branched-chain amino acids.
Literature:
- Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. DOI: 10.1093/pcp/pch099 ; PMID: 15215502
- Application of a high-throughput HPLC-MS/MS assay to Arabidopsis mutant screening; evidence that threonine aldolase plays a role in seed nutritional quality. DOI: 10.1111/j.1365-313X.2004.02140.x ; PMID: 15255874
- AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. DOI: 10.1111/j.1365-313X.2005.02575.x ; PMID: 16359384
- Two Arabidopsis threonine aldolases are nonredundant and compete with threonine deaminase for a common substrate pool. DOI: 10.1105/tpc.106.044958 ; PMID: 17172352
- Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses. DOI: 10.1104/pp.108.115733 ; PMID: 18375600
- Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. DOI: 10.1016/j.diff.2010.02.003 ; PMID: 20219281
- Analysis of protein complexes in Arabidopsis leaves using size exclusion chromatography and label-free protein correlation profiling. DOI: 10.1016/j.jprot.2017.06.004 ; PMID: 28627464
Sequences:
cDNA Sequence
- >AT3G04520.1
CACTTATTTGGTTGGACCGATCTTCTTTCTATAAGATTCCCTGTCGTCATTTCGTATTTGATTTGATCACTCATTCTCTCAACCATTTGAGTCACCCGAAGCACATCTCTTTTTATCTCTTCGCCAAGAAAACAAAAAGCATAGCCTAACAGAACAGAATCTAGTGAGCCAAATCAAGATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGAAGAGAAGAGAAGGCGTTATTATTTTAAGTAATTTCACTCGTTGGACATGTGTAAGGTTGTATTTGAATCTTCATTCAGAATAAGGTTGTATTTGAATCGATTTTCTTGGTTTACAAATGAAAACCTTTAACCAAGAAGTAGAAAACTCTTTCATCAAATTCACTTATACATATAAATAACCAATTATAACCCAATAATTTATTCTTTATAATCCGATTATTCC - >AT3G04520.2
CACTTATTTGGTTGGACCGATCTTCTTTCTATAAGATTCCCTGTCGTCATTTCGTATTTGATTTGATCACTCATTCTCTCAACCATTTGAGTCACCCGAAGCACATCTCTTTTTATCTCTTCGCCAAGAAAACAAAAAGCATAGCCTAACAGAACAGAATCTAGTGAGCCAAATCAAGATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGAAGAGAAGAGAAGGCGTTATTATTTTAAGTAATTTCACTCGTTGGACATGTGTAAGGTTGTATTTGAATCTTCATTCAGAATAAGGTTGTATTTGAATCGATTTTCTTGGTTTACAAATGAAAACCTTTAACCAAGAAGTAGAAAACTCTTTCATCAAATTCACTTATACATATAAATAACCAATTATAACCCAATAATTTATTCTTTATAATCCGATTATTCC - >AT3G04520.3
CACTTATTTGGTTGGACCGATCTTCTTTCTATAAGATTCCCTGTCGTCATTTCGTATTTGATTTGATCACTCATTCTCTCAACCATTTGAGTCACCCGAAGCACATCTCTTTTTATCTCTTCGCCAAGAAAACAAAAAGCATAGCCTAACAGAACAGAATCTAGTGAGCCAAATCAAGATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGAAGAGAAGAGAAGGCGTTATTATTTTAAGTAATTTCACTCGTTGGACATGTGTAAGGTTGTATTTGAATCTTCATTCAGAATAAGGTTGTATTTGAATCGATTTTCTTGGTTTACAAATGAAAACCTTTAACCAAGAAGTAGAAAACTCTTTCATCAAATTCACTTATACATATAAATAACCAATTATAACCCAATAATTTATTCTTTATAATCCGATTATTCC
CDS Sequence
- >AT3G04520.1
ATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGA - >AT3G04520.2
ATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGA - >AT3G04520.3
ATGGTGACGCCAACTACAATTAGAACCGTCGATCTCCGCTCCGACACGGTGACTAAACCAACAGAGTCAATGCGTTCAGCAATGGCAAACGCGGAAGTAGACGACGACGTTTTGGGAAACGATCCAACGGCTCTGCGTTTGGAGAAAGAGGTGGCTGAGATCGCTGGAAAAGAGGCGGCGATGTTTGTTCCGTCAGGGACAATGGGGAATCTGATAAGCGTGTTGGTCCATTGTGATGAGAGAGGGAGTGAAGTGATTCTTGGAGACGACTCTCACATTCATATATATGAAAACGGCGGCGTTTCAAGCCTCGGTGGTGTGCATCCGAGGACGGTGAAGAATGAGGAAGACGGTACGATGGAGATTGGTGCTATTGAAGCTGCCGTGAGGAGTCCCAAAGGAGATCTCCATCATCCGGTTACTAAGCTTATATGTCTTGAGAATACTCAAGCAAATTGTGGAGGTAGATGCCTTCCTATAGAATATATAGACAAAGTCGGAGAATTAGCCAAGAAACATGGTTTGAAGCTTCACATTGACGGAGCTAGGATTTTCAACGCTTCTGTGGCACTTGGAGTTCCCGTGAAGAGGATTGTTCAAGCAGCTGATTCTGTCTCGATCTGTTTGTCGAAAGGTATTGGTGCACCGGTCGGTTCGGTAATCGTGGGAAGCAAGAAGTTCATTACTAAAGCAAGGTGGTTAAGGAAAACATTAGGCGGAGGAATGAGACAGATCGGCGTGCTCTGCGCCGCCGCATTGGTGGCTCTCCATGAAAACGTCGCCAAGCTCGAAGATGACCATAAGAAAGCCAGGGTTTTGGCAGAAGGACTGAACCGGATTGAACGGTTAAGAGTGAACGTTGCAGCTGTGGAAACCAACATCATATACGTAGACATACCAGAAGATCCAAAATTTGGAGCAGAAGAGGCATGTAAGAGCTTGGAAGATGTCGGTGTGCTTGTCATACCCCAAGCTACATTCAGAATCAGGATTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGAAAATATTTCACTCGTGA
Protein Sequence
- >AT3G04520.1
MVTPTTIRTVDLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVAEIAGKEAAMFVPSGTMGNLISVLVHCDERGSEVILGDDSHIHIYENGGVSSLGGVHPRTVKNEEDGTMEIGAIEAAVRSPKGDLHHPVTKLICLENTQANCGGRCLPIEYIDKVGELAKKHGLKLHIDGARIFNASVALGVPVKRIVQAADSVSICLSKGIGAPVGSVIVGSKKFITKARWLRKTLGGGMRQIGVLCAAALVALHENVAKLEDDHKKARVLAEGLNRIERLRVNVAAVETNIIYVDIPEDPKFGAEEACKSLEDVGVLVIPQATFRIRIVLHHQISDVDVEYVLSCFEKIFHS - >AT3G04520.2
MVTPTTIRTVDLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVAEIAGKEAAMFVPSGTMGNLISVLVHCDERGSEVILGDDSHIHIYENGGVSSLGGVHPRTVKNEEDGTMEIGAIEAAVRSPKGDLHHPVTKLICLENTQANCGGRCLPIEYIDKVGELAKKHGLKLHIDGARIFNASVALGVPVKRIVQAADSVSICLSKGIGAPVGSVIVGSKKFITKARWLRKTLGGGMRQIGVLCAAALVALHENVAKLEDDHKKARVLAEGLNRIERLRVNVAAVETNIIYVDIPEDPKFGAEEACKSLEDVGVLVIPQATFRIRIVLHHQISDVDVEYVLSCFEKIFHS - >AT3G04520.3
MVTPTTIRTVDLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVAEIAGKEAAMFVPSGTMGNLISVLVHCDERGSEVILGDDSHIHIYENGGVSSLGGVHPRTVKNEEDGTMEIGAIEAAVRSPKGDLHHPVTKLICLENTQANCGGRCLPIEYIDKVGELAKKHGLKLHIDGARIFNASVALGVPVKRIVQAADSVSICLSKGIGAPVGSVIVGSKKFITKARWLRKTLGGGMRQIGVLCAAALVALHENVAKLEDDHKKARVLAEGLNRIERLRVNVAAVETNIIYVDIPEDPKFGAEEACKSLEDVGVLVIPQATFRIRIVLHHQISDVDVEYVLSCFEKIFHS