Gene Details:

• Gene ID: AT2G40890
• Gene Symbol: CYP98A3, REF8
• Gene Name: cytochrome P450, family 98, subfamily A, polypeptide 3, REDUCED EPIDERMAL FLUORESCENCE 8
• Description: cytochrome P450, family 98, subfamily A, polypeptide 3;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT2G40890.1

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
• PO:0000084  — plant sperm cell — célula espermática o esperma (Spanish, exact), male gamete (exact), microgamete (exact), 植物精子細胞 (Japanese, exact), sperm nucleus (related), sperm cell (broad)

Germplasm Phenotype:

• CS66976  — Homozygous plants show altered phenylpropanoid metabolism: the absence of sinapoylmalate in leaves; accumulation of novel p-coumarate derived soluble secondary metabolites and the deposition of p-hydroxyphenyl lignin. These changes lead to small stature; female sterility; vascular collapse; sensitivity to polysaccharide hydrolases and increased susceptibility to fungal infection.
• cyp98A3  — background Wassilewskija hypocotyls with shortened length and increased diameter, roots had a swollen aspect with increased initiation from the crown and showed reduced growth and gravitropism in soil plant maintained the dwarfed phenotype with a rosette never exceeding 1 to 1.5 cm in diameter, developed a bushy miniature rosette of round leaves, growth of cyp98A3 plants was arrested latest at the onset of primary stem development (2 weeks later than wild-type), showed darker leaf coloration , severe alteration of lignin content and composition - mainly H units (95% to 3.5% in wild-type), ectopic lignification in roots
• ref8  — Reduced fluorescent phenylpropanoid secondary metabolites, reduced lignin, collapsed vessel elements, dwarf

Function-related keywords:

• shoot axis apex ,  leaf lamina base ,  root ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen ,  plant sperm cell

Literature:

• The Arabidopsis REF8 gene encodes the 3-hydroxylase of phenylpropanoid metabolism.   DOI:  10.1046/j.1365-313x.2002.01266.x ;  PMID:  11967091
• Cell cycle-regulated gene expression in Arabidopsis.   DOI:  10.1074/jbc.M207570200 ;  PMID:  12169696
• Transcriptional control of monolignol biosynthesis in Pinus taeda: factors affecting monolignol ratios and carbon allocation in phenylpropanoid metabolism.   DOI:  10.1074/jbc.M112051200 ;  PMID:  11891223
• A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth.   DOI:  10.1104/pp.105.069690 ;  PMID:  16377748
• Mapping the Arabidopsis organelle proteome.   DOI:  10.1073/pnas.0506958103 ;  PMID:  16618929
• The growth reduction associated with repressed lignin biosynthesis in Arabidopsis thaliana is independent of flavonoids.   DOI:  10.1105/tpc.110.074161 ;  PMID:  20511296
• Evolution of a novel phenolic pathway for pollen development.   DOI:  10.1126/science.1174095 ;  PMID:  19779199
• Gene engineering, purification, crystallization and preliminary X-ray diffraction of cytochrome P450 p-coumarate-3-hydroxylase (C3H), the Arabidopsis membrane protein.   DOI:  10.1016/j.pep.2011.04.013 ;  PMID:  21549841
• CYP98A22, a phenolic ester 3'-hydroxylase specialized in the synthesis of chlorogenic acid, as a new tool for enhancing the furanocoumarin concentration in Ruta graveolens.   DOI:  10.1186/1471-2229-12-152 ;  PMID:  22931486
• Protein-protein and protein-membrane associations in the lignin pathway.   DOI:  10.1105/tpc.112.102566 ;  PMID:  23175744
• De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae.   DOI:  10.1186/1475-2859-11-155 ;  PMID:  23216753
• Acyl-lipid metabolism.   DOI:  10.1199/tab.0161 ;  PMID:  23505340
• Chemically induced conditional rescue of the reduced epidermal fluorescence8 mutant of Arabidopsis reveals rapid restoration of growth and selective turnover of secondary metabolite pools.   DOI:  10.1104/pp.113.229393 ;  PMID:  24381065
• Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis.   DOI:  10.1126/science.1241602 ;  PMID:  23950498
• An Arabidopsis gene regulatory network for secondary cell wall synthesis.   DOI:  10.1038/nature14099 ;  PMID:  25533953
• Loss of FERULATE 5-HYDROXYLASE Leads to Mediator-Dependent Inhibition of Soluble Phenylpropanoid Biosynthesis in Arabidopsis.   DOI:  10.1104/pp.15.00294 ;  PMID:  26048881
• The scaffold proteins of lignin biosynthetic cytochrome P450 enzymes.   DOI:  10.1038/s41477-018-0142-9 ;  PMID:  29725099
• Evolution of coumaroyl conjugate 3-hydroxylases in land plants: lignin biosynthesis and defense.   DOI:  10.1111/tpj.14373 ;  PMID:  31038800
• An importin-beta-like protein mediates lignin-modification-induced dwarfism in Arabidopsis.   DOI:  10.1111/tpj.14701 ;  PMID:  31972869
• Transcript and metabolite network perturbations in lignin biosynthetic mutants of Arabidopsis.   DOI:  10.1093/plphys/kiac344 ;  PMID:  35880844
• CYP98A3 from Arabidopsis thaliana is a 3'-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway.   DOI:  10.1074/jbc.M104047200 ;  PMID:  11429408
• Changes in secondary metabolism and deposition of an unusual lignin in the ref8 mutant of Arabidopsis.   DOI:  10.1046/j.1365-313x.2002.01267.x ;  PMID:  11967092
• The Arabidopsis REF8 gene encodes the 3-hydroxylase of phenylpropanoid metabolism.   DOI:  10.1046/j.1365-313x.2002.01266.x ;  PMID:  11967091
• Transcriptional control of monolignol biosynthesis in Pinus taeda: factors affecting monolignol ratios and carbon allocation in phenylpropanoid metabolism.   DOI:  10.1074/jbc.M112051200 ;  PMID:  11891223
• The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions.   DOI:  10.1016/j.phytochem.2004.03.028 ;  PMID:  15276431
• A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth.   DOI:  10.1104/pp.105.069690 ;  PMID:  16377748
• Mapping the Arabidopsis organelle proteome.   DOI:  10.1073/pnas.0506958103 ;  PMID:  16618929
• Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis.   DOI:  10.1074/mcp.M600429-MCP200 ;  PMID:  17317660
• Functional characterization of two p-coumaroyl ester 3'-hydroxylase genes from coffee tree: evidence of a candidate for chlorogenic acid biosynthesis.   DOI:  10.1007/s11103-007-9141-3 ;  PMID:  17333503
• High-yield expression and purification of isotopically labeled cytochrome P450 monooxygenases for solid-state NMR spectroscopy.   DOI:  10.1016/j.bbamem.2007.09.009 ;  PMID:  18005930

Sequences: