Gene Details:

  • Gene ID: AT2G32940
  • Gene Symbol: AGO6
  • Gene Name: ARGONAUTE 6
  • Description: Argonaute family protein;(source:Araport11)
  • TAIR Accession: locus:2059370
  • Genome: Araport11_genome_release
  • Species: Arabidopsis thaliana

Transcripts:

Plant Ontology Annotations:

  • PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
  • PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)

Gene Ontology:

  • GO:0004521  — enables — RNA endonuclease activity
  • GO:0035197  — enables — siRNA binding
  • GO:0035194  — involved in — regulatory ncRNA-mediated post-transcriptional gene silencing
  • GO:0005737  — is active in — cytoplasm
  • GO:0006306  — acts upstream of or within — DNA methylation
  • GO:0051607  — acts upstream of or within — defense response to virus
  • GO:0005634  — located in — nucleus
  • GO:0003723  — enables — RNA binding
  • GO:0030422  — acts upstream of or within — siRNA processing
  • GO:0005737  — located in — cytoplasm

Germplasm Phenotype:

  • CS66095  — greater reduction in DNA methylation at endogenous targets such as AtSN1, MEA-ISR, AtREP2 and SIMPLEHAT2 and lower AtREP2 siRNA levels than in either of the single mutants.
  • CS66096  — reduced DNA methylation at endogenous targets such as AtSN1, MEA-ISR, AtREP2 and SIMPLEHAT2; reduced levels of AtREP2 siRNAs.
  • CS66097  — release of TGS of transgenic RD29A-LUC and the endogenous RD29A gene; reduced DNA methylation at endogenous targets such as AtSN1, MEA-ISR, AtREP2 and SIMPLEHAT2; ; reduced levels of AtREP2 siRNAs
  • CS69754  — Reactivation of GFP fluorescence and reduced DNA methylation of the enhancer region of the GFP target construct (T).
  • CS69755  — Reactivation of GFP fluorescence and reduced DNA methylation of the enhancer region of the GFP target construct (T).
  • CS69756  — Reactivation of GFP fluorescence and reduced DNA methylation of the enhancer region of the GFP target construct (T).
  • CS72560  — defects in small RNA accumulation
  • CS72563  — partial complementation of small RNA accumulation
  • ago6-1  — Almost two-fold reduction in CpNpG and four-fold reduction in asymmetric methylation at the AtSN1 locus but not the 5S rDNA repeat. Reduced accumulation of endogenous heterochromatin-related siRNAs.
  • ago6-1 ago4-1  — Substantial decreases in DNA methylation at CpNpG and asymmetric sites but not at CpG sites with respect to the single mutants alone.

Literature:

Sequences:

cDNA Sequence
  • >AT2G32940.1
    AAGTGAAATATATTTTAATTTTCCCATCACTGGAAACGTTCCCTCCATTCTTTTGCTTGTCTGGGAAACCCAAAGAGACTGTGACGGAGAAGAGAAATGAGCTAAATAAATTCCTCTCTTTGATTCACCGGAACCCTAGAAATCCTCTTCTTAATTCTTTCAAAGATTATTTTCCCCTGGAAGGATGGAGACATCTTCATCTCTGCCACTGTCACCCATAAGTATAGAACCAGAACAGCCCAGCCATCGAGATTATGACATCACAACTAGACGTGGGGTTGGAACCACAGGCAACCCAATAGAATTGTGCACCAATCACTTTAATGTTTCTGTGAGACAGCCGGATGTAGTATTTTACCAGTACACTGTTAGTATCACCACAGAAAACGGTGATGCTGTTGATGGGACAGGAATAAGCAGAAAGCTTATGGACCAACTATTCAAGACTTACTCTTCTGATCTTGATGGTAAAAGGTTGGCATATGATGGAGAGAAGACTTTATATACTGTTGGTCCTCTACCACAGAACGAATTTGACTTTCTAGTCATCGTGGAAGGGTCATTTTCAAAACGTGATTGTGGTGTCTCTGATGGTGGTAGTTCTTCCGGTACTTGCAAGCGATCAAAACGTTCTTTCTTGCCAAGAAGTTACAAGGTTCAGATACATTATGCTGCAGAAATACCGTTGAAGACTGTTCTTGGCACTCAGAGAGGAGCTTATACACCAGATAAGAGTGCTCAGGATGCACTTAGAGTGCTTGACATTGTACTGAGGCAGCAAGCAGCTGAAAGGGGATGCCTTTTGGTCAGACAGGCATTTTTCCATAGCGATGGGCACCCCATGAAAGTTGGAGGAGGTGTTATAGGTATTCGAGGTTTACATTCGAGCTTTCGTCCAACTCATGGAGGACTCTCGCTTAACATTGATGTGTCAACAACAATGATCTTAGAACCTGGTCCAGTTATTGAATTCCTGAAAGCCAATCAGAGTGTTGAGACACCACGGCAAATCGACTGGATTAAGGCTGCGAAAATGCTTAAACATATGAGGGTTAAGGCAACACACCGTAACATGGAATTTAAAATTATAGGTCTAAGTTCGAAACCGTGCAATCAGCAACTGTTTTCAATGAAGATTAAAGATGGTGAACGTGAGGTACCTATTAGAGAGATTACTGTGTATGACTATTTCAAGCAAACCTACACAGAGCCTATTTCTTCTGCGTACTTTCCATGCCTTGATGTTGGCAAGCCAGATCGGCCCAACTATCTTCCACTGGAGTTTTGTAATCTTGTATCTCTGCAACGTTATACAAAACCATTGTCAGGGAGGCAAAGAGTTTTACTTGTTGAAAGTTCAAGACAAAAACCTCTAGAGAGAATTAAAACACTCAATGATGCAATGCATACCTACTGCTATGACAAGGACCCATTTTTGGCTGGATGTGGCATCTCCATTGAAAAAGAAATGACTCAAGTTGAAGGCCGTGTTCTCAAGCCCCCAATGCTGAAGTTTGGCAAGAATGAAGATTTTCAACCTTGCAACGGACGGTGGAACTTTAACAACAAGATGCTTCTAGAACCAAGAGCTATTAAAAGCTGGGCTATCGTCAACTTTTCTTTTCCATGCGACAGTAGTCACATTTCCCGTGAGCTTATAAGCTGTGGCATGAGGAAAGGCATTGAAATTGATCGACCTTTTGCACTAGTTGAAGAGGATCCACAGTACAAAAAAGCAGGTCCTGTTGAAAGGGTAGAAAAAATGATTGCAACGATGAAGTTAAAATTTCCAGATCCTCCTCATTTTATCCTCTGTATTCTGCCAGAACGGAAAACTTCTGATATCTATGGTCCCTGGAAGAAGATATGCCTCACTGAAGAAGGGATCCACACACAATGCATCTGCCCAATCAAGATCAGTGACCAATATCTCACCAATGTACTTCTGAAGATAAATTCGAAGCTTGGAGGCATCAATTCTCTGTTGGGAATAGAGTACTCTTACAACATTCCATTGATAAACAAAATTCCCACCTTGATTTTGGGTATGGATGTATCTCATGGGCCTCCAGGTCGGGCAGATGTTCCTTCAGTAGCAGCGGTTGTTGGTTCAAAATGCTGGCCCTTAATCTCAAGGTATAGGGCAGCTGTAAGAACTCAGTCACCACGTCTGGAGATGATTGATTCGCTCTTCCAACCTATCGAGAACACTGAGAAAGGAGATAACGGTATCATGAACGAATTATTTGTAGAGTTCTATCGGACAAGCAGGGCACGGAAACCTAAGCAGATTATCATTTTCAGGGATGGTGTGAGTGAATCACAGTTCGAGCAAGTCTTGAAGATCGAAGTGGACCAAATTATAAAGGCGTATCAACGTCTTGGTGAATCTGATGTGCCAAAATTCACTGTCATTGTGGCTCAGAAGAACCACCACACCAAGTTGTTTCAAGCTAAGGGTCCTGAAAATGTTCCTGCAGGAACTGTCGTGGACACCAAGATCGTACACCCGACAAACTACGATTTTTACATGTGTGCTCATGCAGGAAAAATAGGAACTTCAAGACCGGCTCATTACCATGTATTACTTGATGAGATTGGTTTCTCCCCCGATGACTTGCAGAATCTCATCCATTCTCTCTCCTATGTCAACCAACGTAGCACAACTGCAACTTCGATCGTGGCTCCAGTACGATACGCTCATCTTGCAGCAGCTCAAGTTGCGCAATTCACAAAGTTTGAAGGTATATCGGAGGACGGGAAAGTTCCAGAGCTTCCACGTCTGCACGAGAATGTCGAAGGCAACATGTTCTTCTGCTGAGTACATATAAGTAGTTCTGCTTCCTCCCTAGTCTAAACCCAAACCCTCCTCTTTAGAGGAAGTTTATCATTTTGCATATATGCAATCATCACCGTACAGTTTCATTAGAAGTAAGAAGAAAGATCCTCGCGTAGATTAAGAGGCTCATTGGCTTATTGATAGCCCAAGTTTGCTTACTAAACGGCAGCGATTTTTGCGTTTAGCTTTTGGATTTTTCAATACGTTATATTAAATTTTCTTTTTTGTTTTGTGACGGGACTCACAAAGGGAAATGAAAAGTGAGAAAAACTCTTGACGTAAAA
CDS Sequence
  • >AT2G32940.1
    ATGGAGACATCTTCATCTCTGCCACTGTCACCCATAAGTATAGAACCAGAACAGCCCAGCCATCGAGATTATGACATCACAACTAGACGTGGGGTTGGAACCACAGGCAACCCAATAGAATTGTGCACCAATCACTTTAATGTTTCTGTGAGACAGCCGGATGTAGTATTTTACCAGTACACTGTTAGTATCACCACAGAAAACGGTGATGCTGTTGATGGGACAGGAATAAGCAGAAAGCTTATGGACCAACTATTCAAGACTTACTCTTCTGATCTTGATGGTAAAAGGTTGGCATATGATGGAGAGAAGACTTTATATACTGTTGGTCCTCTACCACAGAACGAATTTGACTTTCTAGTCATCGTGGAAGGGTCATTTTCAAAACGTGATTGTGGTGTCTCTGATGGTGGTAGTTCTTCCGGTACTTGCAAGCGATCAAAACGTTCTTTCTTGCCAAGAAGTTACAAGGTTCAGATACATTATGCTGCAGAAATACCGTTGAAGACTGTTCTTGGCACTCAGAGAGGAGCTTATACACCAGATAAGAGTGCTCAGGATGCACTTAGAGTGCTTGACATTGTACTGAGGCAGCAAGCAGCTGAAAGGGGATGCCTTTTGGTCAGACAGGCATTTTTCCATAGCGATGGGCACCCCATGAAAGTTGGAGGAGGTGTTATAGGTATTCGAGGTTTACATTCGAGCTTTCGTCCAACTCATGGAGGACTCTCGCTTAACATTGATGTGTCAACAACAATGATCTTAGAACCTGGTCCAGTTATTGAATTCCTGAAAGCCAATCAGAGTGTTGAGACACCACGGCAAATCGACTGGATTAAGGCTGCGAAAATGCTTAAACATATGAGGGTTAAGGCAACACACCGTAACATGGAATTTAAAATTATAGGTCTAAGTTCGAAACCGTGCAATCAGCAACTGTTTTCAATGAAGATTAAAGATGGTGAACGTGAGGTACCTATTAGAGAGATTACTGTGTATGACTATTTCAAGCAAACCTACACAGAGCCTATTTCTTCTGCGTACTTTCCATGCCTTGATGTTGGCAAGCCAGATCGGCCCAACTATCTTCCACTGGAGTTTTGTAATCTTGTATCTCTGCAACGTTATACAAAACCATTGTCAGGGAGGCAAAGAGTTTTACTTGTTGAAAGTTCAAGACAAAAACCTCTAGAGAGAATTAAAACACTCAATGATGCAATGCATACCTACTGCTATGACAAGGACCCATTTTTGGCTGGATGTGGCATCTCCATTGAAAAAGAAATGACTCAAGTTGAAGGCCGTGTTCTCAAGCCCCCAATGCTGAAGTTTGGCAAGAATGAAGATTTTCAACCTTGCAACGGACGGTGGAACTTTAACAACAAGATGCTTCTAGAACCAAGAGCTATTAAAAGCTGGGCTATCGTCAACTTTTCTTTTCCATGCGACAGTAGTCACATTTCCCGTGAGCTTATAAGCTGTGGCATGAGGAAAGGCATTGAAATTGATCGACCTTTTGCACTAGTTGAAGAGGATCCACAGTACAAAAAAGCAGGTCCTGTTGAAAGGGTAGAAAAAATGATTGCAACGATGAAGTTAAAATTTCCAGATCCTCCTCATTTTATCCTCTGTATTCTGCCAGAACGGAAAACTTCTGATATCTATGGTCCCTGGAAGAAGATATGCCTCACTGAAGAAGGGATCCACACACAATGCATCTGCCCAATCAAGATCAGTGACCAATATCTCACCAATGTACTTCTGAAGATAAATTCGAAGCTTGGAGGCATCAATTCTCTGTTGGGAATAGAGTACTCTTACAACATTCCATTGATAAACAAAATTCCCACCTTGATTTTGGGTATGGATGTATCTCATGGGCCTCCAGGTCGGGCAGATGTTCCTTCAGTAGCAGCGGTTGTTGGTTCAAAATGCTGGCCCTTAATCTCAAGGTATAGGGCAGCTGTAAGAACTCAGTCACCACGTCTGGAGATGATTGATTCGCTCTTCCAACCTATCGAGAACACTGAGAAAGGAGATAACGGTATCATGAACGAATTATTTGTAGAGTTCTATCGGACAAGCAGGGCACGGAAACCTAAGCAGATTATCATTTTCAGGGATGGTGTGAGTGAATCACAGTTCGAGCAAGTCTTGAAGATCGAAGTGGACCAAATTATAAAGGCGTATCAACGTCTTGGTGAATCTGATGTGCCAAAATTCACTGTCATTGTGGCTCAGAAGAACCACCACACCAAGTTGTTTCAAGCTAAGGGTCCTGAAAATGTTCCTGCAGGAACTGTCGTGGACACCAAGATCGTACACCCGACAAACTACGATTTTTACATGTGTGCTCATGCAGGAAAAATAGGAACTTCAAGACCGGCTCATTACCATGTATTACTTGATGAGATTGGTTTCTCCCCCGATGACTTGCAGAATCTCATCCATTCTCTCTCCTATGTCAACCAACGTAGCACAACTGCAACTTCGATCGTGGCTCCAGTACGATACGCTCATCTTGCAGCAGCTCAAGTTGCGCAATTCACAAAGTTTGAAGGTATATCGGAGGACGGGAAAGTTCCAGAGCTTCCACGTCTGCACGAGAATGTCGAAGGCAACATGTTCTTCTGCTGA
Protein Sequence
  • >AT2G32940.1
    METSSSLPLSPISIEPEQPSHRDYDITTRRGVGTTGNPIELCTNHFNVSVRQPDVVFYQYTVSITTENGDAVDGTGISRKLMDQLFKTYSSDLDGKRLAYDGEKTLYTVGPLPQNEFDFLVIVEGSFSKRDCGVSDGGSSSGTCKRSKRSFLPRSYKVQIHYAAEIPLKTVLGTQRGAYTPDKSAQDALRVLDIVLRQQAAERGCLLVRQAFFHSDGHPMKVGGGVIGIRGLHSSFRPTHGGLSLNIDVSTTMILEPGPVIEFLKANQSVETPRQIDWIKAAKMLKHMRVKATHRNMEFKIIGLSSKPCNQQLFSMKIKDGEREVPIREITVYDYFKQTYTEPISSAYFPCLDVGKPDRPNYLPLEFCNLVSLQRYTKPLSGRQRVLLVESSRQKPLERIKTLNDAMHTYCYDKDPFLAGCGISIEKEMTQVEGRVLKPPMLKFGKNEDFQPCNGRWNFNNKMLLEPRAIKSWAIVNFSFPCDSSHISRELISCGMRKGIEIDRPFALVEEDPQYKKAGPVERVEKMIATMKLKFPDPPHFILCILPERKTSDIYGPWKKICLTEEGIHTQCICPIKISDQYLTNVLLKINSKLGGINSLLGIEYSYNIPLINKIPTLILGMDVSHGPPGRADVPSVAAVVGSKCWPLISRYRAAVRTQSPRLEMIDSLFQPIENTEKGDNGIMNELFVEFYRTSRARKPKQIIIFRDGVSESQFEQVLKIEVDQIIKAYQRLGESDVPKFTVIVAQKNHHTKLFQAKGPENVPAGTVVDTKIVHPTNYDFYMCAHAGKIGTSRPAHYHVLLDEIGFSPDDLQNLIHSLSYVNQRSTTATSIVAPVRYAHLAAAQVAQFTKFEGISEDGKVPELPRLHENVEGNMFFC