Gene Details:

• Gene ID: AT2G32230
• Gene Symbol: PRORP1
• Gene Name: proteinaceous RNase P 1
• Description: proteinaceous RNase P 1;(source:Araport11)
• TAIR Accession: locus:2045432
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT2G32230.1     AT2G32230.2

Gene Ontology:

• GO:0004526  — enables — ribonuclease P activity
• GO:0001682  — acts upstream of or within — tRNA 5-leader removal GO:0001682 11700 P other metabolic processes IMP RNAi experiments NONE Publication:501763424|PMID:25793367 TAIR 2015-04-21 AT2G32230 locus:2045432 AT2G32230 acts upstream of or within tRNA processing GO:0008033 7397 P other metabolic processes IDA in vitro assay NONE Publication:501737914|PMID:20473316 TAIR 2010-06-04 AT2G32230 locus:2045432 AT2G32230 acts upstream of or within tRNA 5-leader removal
• GO:0001682  — involved in — tRNA 5-leader removal GO:0001682 11700 P nucleobase-containing compound metabolic process IBA none PANTHER:PTN001005190|TAIR:locus:2045432|TAIR:locus:2141662|TAIR:locus:2059824 Communication:501741973 2023-06-07 AT2G32230 locus:2045432 AT2G32230 involved in tRNA 5-leader removal
• GO:0009507  — located in — chloroplast
• GO:0005739  — located in — mitochondrion
• GO:0008033  — acts upstream of or within — tRNA processing

Literature:

• A single Arabidopsis organellar protein has RNase P activity.   DOI:  10.1038/nsmb.1812 ;  PMID:  20473316
• ribonucleases are key players in the control of plastid gene expression.   DOI:  10.1093/jxb/err401 ;  PMID:  22140236
• Mitochondrial ribonuclease P structure provides insight into the evolution of catalytic strategies for precursor-tRNA 5' processing.   DOI:  10.1073/pnas.1209062109 ;  PMID:  22991464
• tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences.   DOI:  10.1002/cbic.201200434 ;  PMID:  22976545
• RNase P enzymes: divergent scaffolds for a conserved biological reaction.   DOI:  10.4161/rna.24513 ;  PMID:  23595059
• Pentatricopeptide repeat motifs in the processing enzyme PRORP1 in Arabidopsis thaliana play a crucial role in recognition of nucleotide bases at TψC loop in precursor tRNAs.   DOI:  10.1016/j.bbrc.2014.07.030 ;  PMID:  25034328
• Mechanistic Studies Reveal Similar Catalytic Strategies for Phosphodiester Bond Hydrolysis by Protein-only and RNA-dependent Ribonuclease P.   DOI:  10.1074/jbc.M115.644831 ;  PMID:  25817998
• Multiple RNA processing defects and impaired chloroplast function in plants deficient in the organellar protein-only RNase P enzyme.   DOI:  10.1371/journal.pone.0120533 ;  PMID:  25793367
• Crystallization and crystallographic analysis of an Arabidopsis nuclear proteinaceous RNase P.   DOI:  10.1107/S2053230X15017033 ;  PMID:  26527263
• Nuclear Protein-Only Ribonuclease P2 Structure and Biochemical Characterization Provide Insight into the Conserved Properties of tRNA 5' End Processing Enzymes.   DOI:  10.1016/j.jmb.2015.11.025 ;  PMID:  26655022
• Differential substrate recognition by isozymes of plant protein-only Ribonuclease P.   DOI:  10.1261/rna.055541.115 ;  PMID:  26966150
• The Restorer-of-fertility-like 2 pentatricopeptide repeat protein and RNase P are required for the processing of mitochondrial orf291 RNA in Arabidopsis.   DOI:  10.1111/tpj.13185 ;  PMID:  27122350
• Use of chemical modification and mass spectrometry to identify substrate-contacting sites in proteinaceous RNase P, a tRNA processing enzyme.   DOI:  10.1093/nar/gkw391 ;  PMID:  27166372
• Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes.   DOI:  10.1093/nar/gkx405 ;  PMID:  28499021
• Cleavage of Model Substrates by Arabidopsis thaliana PRORP1 Reveals New Insights into Its Substrate Requirements.   DOI:  10.1371/journal.pone.0160246 ;  PMID:  27494328
• Molecular recognition of pre-tRNA by Arabidopsis protein-only Ribonuclease P.   DOI:  10.1261/rna.061457.117 ;  PMID:  28874505
• Determination of protein-only RNase P interactome in Arabidopsis mitochondria and chloroplasts identifies a complex between PRORP1 and another NYN domain nuclease.   DOI:  10.1111/tpj.14458 ;  PMID:  31319441
• Genome-wide identification of EMBRYO-DEFECTIVE (EMB) genes required for growth and development in Arabidopsis.   DOI:  10.1111/nph.16071 ;  PMID:  31334862
• Pentatricopeptide repeats of protein-only RNase P use a distinct mode to recognize conserved bases and structural elements of pre-tRNA.   DOI:  10.1093/nar/gkaa627 ;  PMID:  32719843
• Synthetic riboswitches for the analysis of tRNA processing by eukaryotic RNase P enzymes.   DOI:  10.1261/rna.078814.121 ;  PMID:  35022261

Sequences: