Gene Details:

• Gene ID: AT2G29630
• Gene Symbol: PY, THIC
• Gene Name: PYRIMIDINE REQUIRING, thiaminC
• Description: thiaminC;(source:Araport11)
• TAIR Accession:
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT2G29630.1     AT2G29630.2     AT2G29630.3     AT2G29630.4

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)

Germplasm Phenotype:

• CS3335  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of Vitamin B1; 2-5-dimethyl-4-aminopyrimidine or thiamine restores to normal; compact rosette, short petioles, blunt siliques.
• CS3368  — angustifolia, apetala, pyrimidine requiring, erecta, long hypocotyl, glabra, brevipedicellus, eceriferum, transparent testa.
• CS3467  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3470  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); large rosette leaves, edges curled down.
• CS3471  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); rosette leaf edges curled down.
• CS3473  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); rosette leaf edges curled down.
• CS3477  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat).
• CS3480  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3483  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat).
• CS3485  — yellow seeds (lacks brown pigment in seed coat); leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; bright green stems and siliques due to abnormal wax layer.
• CS3490  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of Vitamin B1; 2-5-dimethyl-4-aminopyrimidine or thiamine restores to normal; compact rosette, short petioles, blunt siliques.
• CS3491  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3498  — asymmetric, lobed and curled down rosette leaves; compact rosette, short petioles, blunt siliques; leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3500  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); narrow rosette leaves.
• CS3503  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat).
• CS3510  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3521  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); large rosette leaves, edges curled down.
• CS3522  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3523  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3527  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3536  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3538  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3540  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3544  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); bright green stems and siliques, looks like eceriferum.
• CS3546  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat).
• CS3549  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal.
• CS3551  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat).
• CS3555  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3563  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; trichomes absent on leaves and stems, some trichomes in leaf margins.
• CS3572  — leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; yellow seeds (lacks brown pigment in seed coat); large rosette leaves, edges curled down.
• CS3589  — asymmetric, lobed rosette leaves, leaf edges slightly curled under; compact plants, blunt siliques; leaves but not cotyledons white, conditional lethal; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; high temperature sensitive (28C).
• CS3595  — narrow leaves, slightly twisted siliques; compact rosette and inflorescences, short petioles, blunt siliques; leaves but not cotyledons white/pale; conditional lethal; blocked in the synthesis of the pyrimidine part of vitamin B1; 2,5-dimethyl-4-aminopyrimidine or thiamine restores to normal; lacks trichomes on stems and leaves.
• CS78  — true leaves of unsupplemented plants white or pale.
• thiC  — A severe reduction (~10-fold) of THIC levels in plants decreases vitamin B1 (thiamin diphosphate (TPP)) levels and also leads to changes in the levels of numerous other metabolites since so many primary metabolic enzymes require a TPP co-factor. For example, alanine, asparagine, methionine, phenylalanine, tryptophan, and malate levels rise while fumarate, gentiobiose, and ribose levels decrease. thiC mutants are chlorotic and arrest in their development at the cotyledon stage.The mutant phenotype can be largely rescued by continuous supplementation with thiamin.
• thic1  — thic1 mutants produce white leaves and die early unless they are grown with thiamine supplements. Total thiamine levels are reduced by roughly 60% in the thic1 mutant.

Function-related keywords:

• shoot axis apex ,  leaf lamina base ,  root ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen

Literature:

• Thiamine mutants of the crucifer, Arabidopsis.   DOI:  10.1007/BF00520351 ;  PMID:  5364924
• Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana.   DOI:  10.1074/jbc.M308435200 ;  PMID:  14576160
• When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress.   DOI:  10.1104/pp.103.033431 ;  PMID:  15047901
• Transcriptional profiling implicates novel interactions between abiotic stress and hormonal responses in Thellungiella, a close relative of Arabidopsis.   DOI:  10.1104/pp.105.070508 ;  PMID:  16500996
• Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development.   DOI:  10.1105/tpc.106.047688 ;  PMID:  17337630
• Vitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC.   DOI:  10.1073/pnas.0709597104 ;  PMID:  18048325
• Genome-wide expression profiling Arabidopsis at the stage of Golovinomyces cichoracearum haustorium formation.   DOI:  10.1104/pp.107.111286 ;  PMID:  18218973
• Simultaneous interaction of Arabidopsis thaliana with Bradyrhizobium Sp. strain ORS278 and Pseudomonas syringae pv. tomato DC3000 leads to complex transcriptome changes.   DOI:  10.1094/MPMI-21-2-0244 ;  PMID:  18184068
• AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana.   DOI:  10.1038/cr.2008.35 ;  PMID:  18332905
• An extensive (co-)expression analysis tool for the cytochrome P450 superfamily in Arabidopsis thaliana.   DOI:  10.1186/1471-2229-8-47 ;  PMID:  18433503
• Riboswitch control of gene expression in plants by splicing and alternative 3' end processing of mRNAs.   DOI:  10.1105/tpc.107.053645 ;  PMID:  17993623
• Structural basis of thiamine pyrophosphate analogues binding to the eukaryotic riboswitch.   DOI:  10.1021/ja801708e ;  PMID:  18533652
• Integrating the genetic and physical maps of Arabidopsis thaliana: identification of mapped alleles of cloned essential (EMB) genes.   DOI:  10.1371/journal.pone.0007386 ;  PMID:  19812694
• Alternative splicing expands the repertoire of dominant JAZ repressors of jasmonate signaling.   DOI:  10.1111/j.1365-313X.2010.04265.x ;  PMID:  20525008
• Identification of nuclear genes encoding chloroplast-localized proteins required for embryo development in Arabidopsis.   DOI:  10.1104/pp.110.168120 ;  PMID:  21139083
• The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response.   DOI:  10.1186/1471-2229-12-2 ;  PMID:  22214485
• γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots.   DOI:  10.1111/pce.12033 ;  PMID:  23148892
• Orchestration of thiamin biosynthesis and central metabolism by combined action of the thiamin pyrophosphate riboswitch and the circadian clock in Arabidopsis.   DOI:  10.1105/tpc.112.106385 ;  PMID:  23341335
• High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana.   DOI:  10.1016/j.jsb.2013.10.005 ;  PMID:  24161603
• Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine.   DOI:  10.1042/BJ20140522 ;  PMID:  25014715
• Enhancement of Thiamin Content in Arabidopsis thaliana by Metabolic Engineering.   DOI:  10.1093/pcp/pcv148 ;  PMID:  26454882
• The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins.   DOI:  10.1074/jbc.RA119.011034 ;  PMID:  31911438
• Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.   DOI:  10.1093/plphys/kiab198 ;  PMID:  33944954
• Clock and riboswitch control of THIC in tandem are essential for appropriate gauging of TDP levels under light/dark cycles in Arabidopsis.   DOI:  10.1016/j.isci.2023.106134 ;  PMID:  36866249
• AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana.   DOI:  10.1038/cr.2008.35 ;  PMID:  18332905
• Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.   DOI:  10.1371/journal.pone.0001994 ;  PMID:  18431481
• Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.   DOI:  10.1104/pp.108.124545 ;  PMID:  18633119
• The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts.   DOI:  10.1074/mcp.M500180-MCP200 ;  PMID:  16207701
• Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand.   DOI:  10.1126/science.1128451 ;  PMID:  16675665
• Metabolite-binding RNA domains are present in the genes of eukaryotes.   DOI:  10.1261/rna.5090103 ;  PMID:  12756322
• AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana.   DOI:  10.1038/cr.2008.35 ;  PMID:  18332905
• Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.   DOI:  10.1371/journal.pone.0001994 ;  PMID:  18431481

Sequences: