Gene Details:

• Gene ID: AT2G29090
• Gene Symbol: CYP707A2
• Gene Name: cytochrome P450, family 707, subfamily A, polypeptide 2
• Description: cytochrome P450, family 707, subfamily A, polypeptide 2;(source:Araport11)
• TAIR Accession: locus:2066138
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT2G29090.1     AT2G29090.2     AT2G29090.4     AT2G29090.3

Plant Ontology Annotations:

• PO:0000014  — rosette leaf — hoja en roseta (Spanish, exact), ロゼット葉 (Japanese, exact)
• PO:0000056  — flower bud — floral bud (exact), yema floral (Spanish, exact), 花芽 (Japanese, exact)
• PO:0009001  — fruit — frucht (exact, German), fruto (exact, Spanish), 果実 (exact, Japanese), coenocarp (narrow), syncarp (narrow), aggregate fruit (broad), compound fruit (broad), dehiscent fruit (broad), diaspore (broad), indehiscent fruit (broad), multiple fruit (broad), propagule (broad)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009047  — stem — caña (Spanish, exact), culm (exact), eje primario (Spanish, exact), primary axis (exact), primary stem (exact), tallo (Spanish, exact), tronco (Spanish, exact), 茎 (Japanese, exact), bole (narrow), cane (narrow), caudex (narrow), caudices (narrow), core (narrow), primocane (narrow), scape (narrow), stalk (narrow), trunk (narrow)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)
• PO:0005014  — embryo cortex — cortex (corteza) del emrbion (Spanish, exact), 胚皮層 (Japanese, exact)
• PO:0005015  — embryo endodermis — endodermis del embrión (Spanish, exact), 胚内胚葉 (Japanese, exact)
• PO:0008011  — plant embryo vascular system — sistema vascular del embrión (Spanish, exact), 胚維管系 (Japanese, exact)
• PO:0009089  — endosperm — albumen (exact), endosperma (Spanish, exact), 内胚乳、胚乳 (Japanese, exact)
• PO:0009010  — seed — semilla (Spanish, exact), 種子 (Japanese, exact), pyrene (narrow), diaspore (broad)

Gene Ontology:

• GO:0020037  — enables — heme binding
• GO:0048838  — acts upstream of or within — release of seed from dormancy
• GO:0009687  — acts upstream of or within — abscisic acid metabolic process
• GO:0005506  — enables — iron ion binding
• GO:0016705  — enables — oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0046345  — involved in — abscisic acid catabolic process
• GO:0016125  — involved in — sterol metabolic process

Germplasm Phenotype:

• cyp707a1-1 cyp707a2-1  — ABA levels in dry seeds are 50-fold higher compared to those in wild type.
• cyp707a2-1  — 12h after imbibition, the decrease in ABA content in the mutant seeds was much slower than that of wild type (ca. 70%).
• cyp707a2-1  — ABA levels in dry seeds are 5-fold higher compared to those in wild type.
• cyp707a2-1  — Germination of mutant seeds after dark imbibition and far red/red light treatment was slower than that of wild type seeds.
• cyp707a2-1  — Only 10% of mutant seeds germinate immediately after harvest, compared to 70% for wild type Columbia (reported to have low dormancy). This mutant has higher levels of dormancy.
• cyp707a2-1  — The induction of GA biosynthesis and GA upregulated genes after irradiation with red light is inhibited in the mutant compared to wild type.
• cyp707a2-1 cyp707a3-1  — ABA levels in dry seeds are 5-fold higher compared to those in wild type.

Function-related keywords:

• rosette leaf ,  flower bud ,  fruit ,  root ,  flower ,  stem ,  leaf lamina base ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  collective leaf structure ,  pollen ,  embryo cortex ,  embryo endodermis ,  plant embryo vascular system ,  plant embryo ,  plant embryo ,  endosperm ,  seed

Literature:

• Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid.   DOI:  10.1104/pp.103.037614 ;  PMID:  15064374
• Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis.   DOI:  10.1073/pnas.0305876101 ;  PMID:  15148388
• Abscisic acid biosynthesis and catabolism.   DOI:  10.1146/annurev.arplant.56.032604.144046 ;  PMID:  15862093
• CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis.   DOI:  10.1104/pp.106.079475 ;  PMID:  16543410
• Seed dormancy and ABA metabolism in Arabidopsis and barley: the role of ABA 8'-hydroxylase.   DOI:  10.1111/j.1365-313X.2006.02659.x ;  PMID:  16507085
• Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism.   DOI:  10.1111/j.1365-313X.2006.02881.x ;  PMID:  17010113
• PIL5, a phytochrome-interacting bHLH protein, regulates gibberellin responsiveness by binding directly to the GAI and RGA promoters in Arabidopsis seeds.   DOI:  10.1105/tpc.107.050153 ;  PMID:  17449805
• SOMNUS, a CCCH-type zinc finger protein in Arabidopsis, negatively regulates light-dependent seed germination downstream of PIL5.   DOI:  10.1105/tpc.108.058859 ;  PMID:  18487351
• The Arabidopsis abscisic acid catabolic gene CYP707A2 plays a key role in nitrate control of seed dormancy.   DOI:  10.1104/pp.108.126938 ;  PMID:  19074630
• Nitric oxide-induced rapid decrease of abscisic acid concentration is required in breaking seed dormancy in Arabidopsis.   DOI:  10.1111/j.1469-8137.2009.02899.x ;  PMID:  19522839
• Antagonism between abscisic acid and ethylene in Arabidopsis acts in parallel with the reciprocal regulation of their metabolism and signaling pathways.   DOI:  10.1007/s11103-009-9509-7 ;  PMID:  19513806
• Major flowering time gene, flowering locus C, regulates seed germination in Arabidopsis thaliana.   DOI:  10.1073/pnas.0901367106 ;  PMID:  19564609
• Rapid accumulation of NO regulates ABA catabolism and seed dormancy during imbibition in Arabidopsis.   DOI:  10.4161/psb.4.9.9532 ;  PMID:  19847111
• Genome-wide analysis of endogenous abscisic acid-mediated transcription in dry and imbibed seeds of Arabidopsis using tiling arrays.   DOI:  10.1111/j.1365-313X.2010.04135.x ;  PMID:  20088898
• H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.   DOI:  10.1093/jxb/erq125 ;  PMID:  20460363
• ABA 9'-hydroxylation is catalyzed by CYP707A in Arabidopsis.   DOI:  10.1016/j.phytochem.2011.02.004 ;  PMID:  21414645
• Involvement of the abscisic acid catabolic gene CYP707A2 in the glucose-induced delay in seed germination and post-germination growth of Arabidopsis.   DOI:  10.1111/j.1399-3054.2011.01510.x ;  PMID:  21883251
• Dormancy cycling in Arabidopsis seeds is controlled by seasonally distinct hormone-signaling pathways.   DOI:  10.1073/pnas.1116325108 ;  PMID:  22128331
• The Arabidopsis mutant, fy-1, has an ABA-insensitive germination phenotype.   DOI:  10.1093/jxb/err452 ;  PMID:  22282534
• Gene expression profiling through microarray analysis in Arabidopsis thaliana colonized by Pseudomonas putida MTCC5279, a plant growth promoting rhizobacterium.   DOI:  10.4161/psb.18957 ;  PMID:  22353860
• ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination.   DOI:  10.3389/fpls.2013.00063 ;  PMID:  23531630
• Temperature, light and nitrate sensing coordinate Arabidopsis seed dormancy cycling, resulting in winter and summer annual phenotypes.   DOI:  10.1111/tpj.12186 ;  PMID:  23590427
• ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis.   DOI:  10.1371/journal.pgen.1003577 ;  PMID:  23818868
• Abscisic acid uridine diphosphate glucosyltransferases play a crucial role in abscisic acid homeostasis in Arabidopsis.   DOI:  10.1104/pp.114.239210 ;  PMID:  24676855
• Arabidopsis cold shock domain protein 2 influences ABA accumulation in seed and negatively regulates germination.   DOI:  10.1016/j.bbrc.2014.11.092 ;  PMID:  25475723
• Mutations in the Arabidopsis Lst8 and Raptor genes encoding partners of the TOR complex, or inhibition of TOR activity decrease abscisic acid (ABA) synthesis.   DOI:  10.1016/j.bbrc.2015.10.028 ;  PMID:  26459592
• Interplay between ABA and GA Modulates the Timing of Asymmetric Cell Divisions in the Arabidopsis Root Ground Tissue.   DOI:  10.1016/j.molp.2016.02.009 ;  PMID:  26970019
• NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.   DOI:  10.1038/ncomms13179 ;  PMID:  27731416
• The DAG1 transcription factor negatively regulates the seed-to-seedling transition in Arabidopsis acting on ABA and GA levels.   DOI:  10.1186/s12870-016-0890-5 ;  PMID:  27613195
• AtPER1 enhances primary seed dormancy and reduces seed germination by suppressing the ABA catabolism and GA biosynthesis in Arabidopsis seeds.   DOI:  10.1111/tpj.14542 ;  PMID:  31536657
• Histone Deacetylase HDA9 With ABI4 Contributes to Abscisic Acid Homeostasis in Drought Stress Response.   DOI:  10.3389/fpls.2020.00143 ;  PMID:  32158458
• Nitrate Promotes Germination Under Inhibition by NaCl or High Concentration of Glucose.   DOI:  10.3390/plants9060707 ;  PMID:  32498308
• The H3K27me3 Demethylase RELATIVE OF EARLY FLOWERING6 Suppresses Seed Dormancy by Inducing Abscisic Acid Catabolism.   DOI:  10.1104/pp.20.01255 ;  PMID:  33037128
• The phytochrome interacting proteins ERF55 and ERF58 repress light-induced seed germination in Arabidopsis thaliana.   DOI:  10.1038/s41467-022-29315-3 ;  PMID:  35351902
• Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis.   DOI:  10.1111/tpj.16102 ;  PMID:  36625794
• The Arabidopsis pre-mRNA 3' end processing related protein FIP1 promotes seed dormancy via the DOG1 and ABA pathways.   DOI:  10.1111/tpj.16239 ;  PMID:  37035898
• Histone Deacetylases Regulate MORE AXILLARY BRANCHED 2-Dependent Germination of Arabidopsis thaliana.   DOI:  10.1093/pcp/pcad047 ;  PMID:  37279553
• SOMNUS, a CCCH-type zinc finger protein in Arabidopsis, negatively regulates light-dependent seed germination downstream of PIL5.   DOI:  10.1105/tpc.108.058859 ;  PMID:  18487351

Sequences: