Gene Details:

• Gene ID: AT2G27040
• Gene Symbol: AGO4, OCP11
• Gene Name: ARGONAUTE 4, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11
• Description: Argonaute family protein;(source:Araport11)
• TAIR Accession: locus:2059258
• Genome: Araport11_genome_release
• Species: Arabidopsis thaliana

Transcripts:

• AT2G27040.1     AT2G27040.2

Plant Ontology Annotations:

• PO:0000037  — shoot axis apex — ápice del epiblasto (epiblastema) (Spanish, exact), シュート頂、茎頂 (Japanese, exact)
• PO:0008019  — leaf lamina base — base de la lámina de la hoja (Spanish, exact), 葉身基部 (Japanese, exact)
• PO:0009005  — root — raíz (Spanish, exact), radices (exact, plural), radix (exact), 根 (Japanese, exact), aerial root (narrow), climbing root (narrow)
• PO:0009006  — shoot system — sistema de epiblasto (epiblastema) (Spanish, exact), シュート系、苗条系 (Japanese, exact), Poaceae crown (related), shoot (related), thalli (related), thallus (related), tree crown (narrow)
• PO:0009009  — plant embryo — embrión (Spanish, exact), 植物胚 (Japanese, exact), germ (related), embryo (broad)
• PO:0009025  — vascular leaf — foliage leaf (exact), hoja vascular (Spanish, exact), leaf, vascular (exact), vascular leaves (exact, plural), 維管束のある葉, または維管束植物の葉 (Japanese, exact), crozier (related), macrophyll (related), megaphyll (related), ascidia (narrow), ascidium (narrow), fiddlehead (narrow), frond (narrow), needle-like leaf (narrow), pitcher (narrow), pitcher blade (narrow), pitcher-blade (narrow), scale-like leaf (narrow), sterile frond (narrow), trophophyll (narrow)
• PO:0009029  — stamen — estambre (Spanish, exact), 雄蕊 (Japanese, exact), Poaceae stamen (narrow), Zea stamen (narrow)
• PO:0009031  — sepal — sépalo (Spanish, exact), がく片 (Japanese, exact)
• PO:0009046  — flower — flor (Spanish, exact), 花 (Japanese, exact), floret (related), Asteraceae floret (narrow), basal flower (narrow), double flower (narrow), hermaphrodite flower (narrow), monoclinous flower (narrow), perfect flower (narrow)
• PO:0009052  — inflorescence flower pedicel — 小花柄 (Japanese, related), pedicelo (Spanish, broad)
• PO:0020030  — cotyledon — cotiledón (Spanish, exact), seed leaf (exact), 子葉 (Japanese, exact)
• PO:0020038  — petiole — pecíolo (Spanish, exact), 葉柄 (Japanese, exact)
• PO:0020100  — hypocotyl — hipocótile (Spanish, exact), 胚軸 (Japanese, exact)
• PO:0020137  — leaf apex — ápice de la hoja (Spanish, exact), 葉先 (Japanese, exact), leaf lamina apex (narrow), phyllid apex (narrow)
• PO:0025022  — collective leaf structure — estructura colectiva de hoja (Spanish, exact), leaf series (exact), 葉が集まった構造 (Japanese, exact), leaf whorl (narrow), rosette (narrow), cycle (broad), verticil (broad)
• PO:0025281  — pollen — polen (Spanish, exact), pollen grain (exact), 花粉 (Japanese, exact)

Gene Ontology:

• GO:0035194  — involved in — regulatory ncRNA-mediated post-transcriptional gene silencing
• GO:0005730  — located in — nucleolus
• GO:0031048  — acts upstream of or within — regulatory ncRNA-mediated heterochromatin formation
• GO:0005737  — located in — cytoplasm
• GO:0042742  — acts upstream of or within — defense response to bacterium
• GO:0051607  — acts upstream of or within — defense response to virus
• GO:0003682  — enables — chromatin binding
• GO:0004521  — enables — RNA endonuclease activity
• GO:0030422  — acts upstream of or within — siRNA processing
• GO:0006306  — acts upstream of or within — DNA methylation
• GO:0005515  — enables — protein binding
• GO:0031507  — involved in — heterochromatin formation
• GO:0015030  — located in — Cajal body
• GO:0000791  — located in — euchromatin
• GO:0005737  — is active in — cytoplasm
• GO:0003723  — enables — RNA binding
• GO:0035197  — enables — siRNA binding
• GO:0005654  — located in — nucleoplasm

Function-related keywords:

• shoot axis apex ,  leaf lamina base ,  root ,  shoot system ,  plant embryo ,  vascular leaf ,  stamen ,  sepal ,  flower ,  inflorescence flower pedicel ,  cotyledon ,  petiole ,  hypocotyl ,  leaf apex ,  collective leaf structure ,  pollen

Literature:

• AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals.   DOI:  10.1073/pnas.200217597 ;  PMID:  11016954
• ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation.   DOI:  10.1126/science.1079695 ;  PMID:  12522258
• Chromatin and siRNA pathways cooperate to maintain DNA methylation of small transposable elements in Arabidopsis.   DOI:  10.1186/gb-2005-6-11-r90 ;  PMID:  16277745
• An ARGONAUTE4-containing nuclear processing center colocalized with Cajal bodies in Arabidopsis thaliana.   DOI:  10.1016/j.cell.2006.05.032 ;  PMID:  16839879
• The Arabidopsis chromatin-modifying nuclear siRNA pathway involves a nucleolar RNA processing center.   DOI:  10.1016/j.cell.2006.05.031 ;  PMID:  16839878
• Two-step recruitment of RNA-directed DNA methylation to tandem repeats.   DOI:  10.1371/journal.pbio.0040363 ;  PMID:  17105345
• Role of RNA polymerase IV in plant small RNA metabolism.   DOI:  10.1073/pnas.0611456104 ;  PMID:  17360559
• Role of Arabidopsis AGO6 in siRNA accumulation, DNA methylation and transcriptional gene silencing.   DOI:  10.1038/sj.emboj.7601603 ;  PMID:  17332757
• Cell biology of the Arabidopsis nuclear siRNA pathway for RNA-directed chromatin modification.   DOI:  10.1101/sqb.2006.71.046 ;  PMID:  17381329
• Regulation of Arabidopsis thaliana 5S rRNA Genes.   DOI:  10.1093/pcp/pcm043 ;  PMID:  17412735
• and intercellular RNA interference in Arabidopsis thaliana requires components of the microRNA and heterochromatic silencing pathways.   DOI:  10.1038/ng2081 ;  PMID:  17558406
• Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis.   DOI:  10.1073/pnas.0706701104 ;  PMID:  17785412
• Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.   DOI:  10.1101/gad.451207 ;  PMID:  17938239
• ARGONAUTE4 is required for resistance to Pseudomonas syringae in Arabidopsis.   DOI:  10.1105/tpc.107.054494 ;  PMID:  17993621
• Viral genome methylation as an epigenetic defense against geminiviruses.   DOI:  10.1128/JVI.00719-08 ;  PMID:  18596098
• NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation.   DOI:  10.1101/gad.1765209 ;  PMID:  19204117
• Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation.   DOI:  10.1038/nature05198 ;  PMID:  16998468
• RNA Pol IV plays catch with Argonaute 4.   DOI:  10.1016/j.cell.2007.10.044 ;  PMID:  18022355
• Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana.   DOI:  10.1371/journal.pgen.0040027 ;  PMID:  18266474
• Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide.   DOI:  10.1016/j.cell.2008.02.034 ;  PMID:  18342361
• RNA polymerase V transcription guides ARGONAUTE4 to chromatin.   DOI:  10.1038/ng.365 ;  PMID:  19377477
• and RNA-binding protein.   DOI:  10.1016/j.cell.2009.04.028 ;  PMID:  19410546
• RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family.   DOI:  10.1038/embor.2009.31 ;  PMID:  19343051
• RNA polymerase V functions in Arabidopsis interphase heterochromatin organization independently of the 24-nt siRNA-directed DNA methylation pathway.   DOI:  10.1093/mp/ssp006 ;  PMID:  19825650
• A Pelargonium ARGONAUTE4 gene shows organ-specific expression and differences in RNA and protein levels.   DOI:  10.1016/j.jplph.2009.10.004 ;  PMID:  19879665
• Intergenic transcription by RNA polymerase II coordinates Pol IV and Pol V in siRNA-directed transcriptional gene silencing in Arabidopsis.   DOI:  10.1101/gad.1868009 ;  PMID:  19948763
• The Arabidopsis RNA-directed DNA methylation argonautes functionally diverge based on their expression and interaction with target loci.   DOI:  10.1105/tpc.109.072199 ;  PMID:  20173091
• and AGO4-associated protein acts in RNA-directed DNA methylation.   DOI:  10.1038/nature09025 ;  PMID:  20410883
• JMJ14, a JmjC domain protein, is required for RNA silencing and cell-to-cell movement of an RNA silencing signal in Arabidopsis.   DOI:  10.1101/gad.579910 ;  PMID:  20478993
• siRNAs from miRNA sites mediate DNA methylation of target genes.   DOI:  10.1093/nar/gkq590 ;  PMID:  20621980
• A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA.   DOI:  10.1371/journal.pgen.1000690 ;  PMID:  19834541
• Transcriptional silencing induced by Arabidopsis T-DNA mutants is associated with 35S promoter siRNAs and requires genes involved in siRNA-mediated chromatin silencing.   DOI:  10.1111/j.1365-313X.2010.04358.x ;  PMID:  21070421
• Computational dissection of Arabidopsis smRNAome leads to discovery of novel microRNAs and short interfering RNAs associated with transcription start sites.   DOI:  10.1016/j.ygeno.2011.01.006 ;  PMID:  21295131
• Characterization of transcriptome remodeling during cambium formation identifies MOL1 and RUL1 as opposing regulators of secondary growth.   DOI:  10.1371/journal.pgen.1001312 ;  PMID:  21379334
• Deep sequencing of small RNAs specifically associated with Arabidopsis AGO1 and AGO4 uncovers new AGO functions.   DOI:  10.1111/j.1365-313X.2011.04594.x ;  PMID:  21457371
• Independent chromatin binding of ARGONAUTE4 and SPT5L/KTF1 mediates transcriptional gene silencing.   DOI:  10.1371/journal.pgen.1002120 ;  PMID:  21738482
• AGO6 functions in RNA-mediated transcriptional gene silencing in shoot and root meristems in Arabidopsis thaliana.   DOI:  10.1371/journal.pone.0025730 ;  PMID:  21998686
• Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein.   DOI:  10.1105/tpc.111.092718 ;  PMID:  22247253
• The RNA silencing enzyme RNA polymerase v is required for plant immunity.   DOI:  10.1371/journal.pgen.1002434 ;  PMID:  22242006
• Systematic identification of functional plant modules through the integration of complementary data sources.   DOI:  10.1104/pp.112.196725 ;  PMID:  22589469
• Non-Canonical Processing of Arabidopsis pri-miR319a/b/c Generates Additional microRNAs to Target One RAP2.12 mRNA Isoform.   DOI:  10.3389/fpls.2012.00046 ;  PMID:  22639648
• Cytoplasmic assembly and selective nuclear import of Arabidopsis Argonaute4/siRNA complexes.   DOI:  10.1016/j.molcel.2012.04.013 ;  PMID:  22608924
• Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis.   DOI:  10.1073/pnas.1208557109 ;  PMID:  22733760
• Structural insights into small RNA sorting and mRNA target binding by Arabidopsis Argonaute Mid domains.   DOI:  10.1016/j.febslet.2012.06.038 ;  PMID:  22771475
• Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation.   DOI:  10.1104/pp.112.200279 ;  PMID:  22846193
• RNA polymerase V targets transcriptional silencing components to promoters of protein-coding genes.   DOI:  10.1111/tpj.12034 ;  PMID:  23013441
• Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome.   DOI:  10.1016/j.cell.2012.10.054 ;  PMID:  23313553
• Generation of a luciferase-based reporter for CHH and CG DNA methylation in Arabidopsis thaliana.   DOI:  10.1186/1758-907X-4-1 ;  PMID:  23561294
• The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs.   DOI:  10.1093/nar/gkt639 ;  PMID:  23877244
• Small RNA pathways and diversity in model legumes: lessons from genomics.   DOI:  10.3389/fpls.2013.00236 ;  PMID:  23847640
• Arabidopsis double-stranded RNA binding protein DRB3 participates in methylation-mediated defense against geminiviruses.   DOI:  10.1128/JVI.02305-13 ;  PMID:  24352449
• Characterizing RNA-protein interaction using cross-linking and metabolite supplemented nuclear RNA-immunoprecipitation.   DOI:  10.1007/s11033-014-3154-1 ;  PMID:  24493449
• RNA-directed DNA methylation requires stepwise binding of silencing factors to long non-coding RNA.   DOI:  10.1111/tpj.12563 ;  PMID:  24862207
• Molecular mechanism of action of plant DRM de novo DNA methyltransferases.   DOI:  10.1016/j.cell.2014.03.056 ;  PMID:  24855943
• Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo.   DOI:  10.1128/JVI.01404-14 ;  PMID:  25100851
• DNA demethylases target promoter transposable elements to positively regulate stress responsive genes in Arabidopsis.   DOI:  10.1186/s13059-014-0458-3 ;  PMID:  25228471
• Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants.   DOI:  10.1093/jxb/eru447 ;  PMID:  25385769
• Specific but interdependent functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA methylation.   DOI:  10.15252/embj.201489453 ;  PMID:  25527293
• The rolB gene activates the expression of genes encoding microRNA processing machinery.   DOI:  10.1007/s10529-014-1743-7 ;  PMID:  25491479
• DICER-LIKE1 processed trans-acting siRNAs mediate DNA methylation: case study of complex small RNA biogenesis and action pathways in plants.   DOI:  10.4161/psb.22476 ;  PMID:  23104109
• The Splicing Factor PRP31 Is Involved in Transcriptional Gene Silencing and Stress Response in Arabidopsis.   DOI:  10.1016/j.molp.2015.02.003 ;  PMID:  25684655
• Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing.   DOI:  10.1105/tpc.15.00264 ;  PMID:  26023161
• A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.   DOI:  10.1016/j.molcel.2015.11.015 ;  PMID:  26711010
• DNA Damage-Induced Transcription of Transposable Elements and Long Non-coding RNAs in Arabidopsis Is Rare and ATM-Dependent.   DOI:  10.1016/j.molp.2016.04.015 ;  PMID:  27150037
• Arabidopsis AGO3 predominantly recruits 24-nt small RNAs to regulate epigenetic silencing.   DOI:  10.1038/nplants.2016.49 ;  PMID:  27243648
• Accurate Chromosome Segregation at First Meiotic Division Requires AGO4, a Protein Involved in RNA-Dependent DNA Methylation in Arabidopsis thaliana.   DOI:  10.1534/genetics.116.189217 ;  PMID:  27466226
• Global identification and expression analysis of stress-responsive genes of the Argonaute family in apple.   DOI:  10.1007/s00438-016-1236-6 ;  PMID:  27475441
• DNA DAMAGE BINDING PROTEIN2 Shapes the DNA Methylation Landscape.   DOI:  10.1105/tpc.16.00474 ;  PMID:  27531226
• A Multigenic Network of ARGONAUTE4 Clade Members Controls Early Megaspore Formation in Arabidopsis.   DOI:  10.1534/genetics.116.188151 ;  PMID:  27591749
• A Primary Sequence Analysis of the ARGONAUTE Protein Family in Plants.   DOI:  10.3389/fpls.2016.01347 ;  PMID:  27635128
• Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana.   DOI:  10.1242/bio.017244 ;  PMID:  27659688
• Antiviral Defense Involves AGO4 in an Arabidopsis-Potexvirus Interaction.   DOI:  10.1094/MPMI-09-16-0188-R ;  PMID:  27762650
• Long non-coding RNA produced by RNA polymerase V determines boundaries of heterochromatin.   DOI:  10.7554/eLife.19092 ;  PMID:  27779094
• Global Genome Repair factors controls DNA methylation patterns in Arabidopsis.   DOI:  10.1080/15592324.2016.1253648 ;  PMID:  27813706
• AGO4 is specifically required for heterochromatic siRNA accumulation at Pol V-dependent loci in Arabidopsis thaliana.   DOI:  10.1111/tpj.13463 ;  PMID:  28002617
• Transcriptome-Wide Discovery of PASRs (Promoter-Associated Small RNAs) and TASRs (Terminus-Associated Small RNAs) in Arabidopsis thaliana.   DOI:  10.1371/journal.pone.0169212 ;  PMID:  28046132
• AtMBD6, a methyl CpG binding domain protein, maintains gene silencing in Arabidopsis by interacting with RNA binding proteins.   DOI:  10.1007/s12038-016-9658-1 ;  PMID:  28229965
• Abscisic Acid Induces Resistance against Bamboo Mosaic Virus through Argonaute2 and 3.   DOI:  10.1104/pp.16.00015 ;  PMID:  28270624
• Long-range control of gene expression via RNA-directed DNA methylation.   DOI:  10.1371/journal.pgen.1006749 ;  PMID:  28475589
• Analysis of Argonaute 4-Associated Long Non-Coding RNA in Arabidopsis thaliana Sheds Novel Insights into Gene Regulation through RNA-Directed DNA Methylation.   DOI:  10.3390/genes8080198 ;  PMID:  28783101
• Dual control of ROS1-mediated active DNA demethylation by DNA damage-binding protein 2 (DDB2).   DOI:  10.1111/tpj.13753 ;  PMID:  29078035
• RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis.   DOI:  10.1038/s41477-017-0100-y ;  PMID:  29379150
• Analysis of the DNA methylation patterns and transcriptional regulation of the NB-LRR-encoding gene family in Arabidopsis thaliana.   DOI:  10.1007/s11103-018-0715-z ;  PMID:  29525832
• Arabidopsis mutants may represent recombinant introgression lines.   DOI:  10.1186/s13104-018-3326-5 ;  PMID:  29615117
• APURINIC/APYRIMIDINIC ENDONUCLEASE2 and ZINC FINGER DNA 3'-PHOSPHOESTERASE Play Overlapping Roles in the Maintenance of Epigenome and Genome Stability.   DOI:  10.1105/tpc.18.00287 ;  PMID:  30135084
• Maternal small RNAs mediate spatial-temporal regulation of gene expression, imprinting, and seed development in Arabidopsis.   DOI:  10.1073/pnas.1807621116 ;  PMID:  30692258
• Analysis of siRNA Precursors Generated by RNA Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis.   DOI:  10.1007/978-1-4939-9045-0_2 ;  PMID:  30945177
• Chemical enhancers of posttranscriptional gene silencing in Arabidopsis.   DOI:  10.1261/rna.068627.118 ;  PMID:  31164480
• Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis.   DOI:  10.1111/mpp.12850 ;  PMID:  31274236
• ARGONAUTE1 and ARGONAUTE4 Regulate Gene Expression and Hypoxia Tolerance.   DOI:  10.1104/pp.19.00741 ;  PMID:  31358683
• Spaceflight induces novel regulatory responses in Arabidopsis seedling as revealed by combined proteomic and transcriptomic analyses.   DOI:  10.1186/s12870-020-02392-6 ;  PMID:  32460700
• Arabidopsis cell wall composition determines disease resistance specificity and fitness.   DOI:  10.1073/pnas.2010243118 ;  PMID:  33509925
• Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis.   DOI:  10.1007/s00497-021-00434-z ;  PMID:  34812935
• Origin, evolution and diversification of plant ARGONAUTE proteins.   DOI:  10.1111/tpj.15615 ;  PMID:  34845788
• Role of the RNA-directed DNA Methylation pathway in the regulation of maternal effects in Arabidopsis thaliana seed germination.   DOI:  10.17912/micropub.biology.000504 ;  PMID:  34901781
• The RNA-binding protein RBP45D of Arabidopsis promotes transgene silencing and flowering time.   DOI:  10.1111/tpj.15637 ;  PMID:  34919766
• The novel activity of Argonautes in intron splicing: A transcriptome-wide survey in plants.   DOI:  10.1016/j.jplph.2022.153632 ;  PMID:  35114616
• The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.   DOI:  10.1093/nar/gkac1135 ;  PMID:  36477368
• Genomic methylation patterns in pre-meiotic gynoecia of wild-type and RdDM mutants of Arabidopsis.   DOI:  10.3389/fpls.2023.1123211 ;  PMID:  36993852
• RETINOBLASTOMA-RELATED interactions with key factors of the RNA-directed DNA methylation (RdDM) pathway and its influence on root development.   DOI:  10.1007/s00425-023-04135-x ;  PMID:  37120771
• Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.   DOI:  10.1021/pr1009433 ;  PMID:  21166475

Sequences: