Gene Details:
Functional Descriptions:
- We suggested that mutation of the seed-type NtFAD2–2 genes will not affect the fatty acid desaturation in tobacco leaves, because of the function redundancy of NtFAD2–1 genes in vegetative tissues.
- Our results indicated that seed-type NtFAD2–2 genes were ideal targets for high oleic acid tobacco seed oil breeding.
- Our results demonstrated that knockout of the tobacco seed-type NtFAD2–2 genes using the CRISPR-Cas9 system could get a stable and high oleic acid tobacco seed oil.
- To further investigate the role of NtFAD2–2 genes in tobacco seed fatty acid desaturation and to construct a high oleic acid tobacco seed oil material, we generated NtFAD2–2 knockout lines using CRISPR-Cas9-based genome editing method.
Function-related keywords:
- seed , vegetative , breeding , fatty-acid
Literature:
- Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2-2. DOI: 10.1186/s12870-020-02441-0 ; PMID: 32450806
Related News:
Gene Resources:
- NCBI ID: NM_001326113
- UniProt accessions:
Orthologs:
Sequences:
- CDS Sequence:
- Protein Sequence: