Gene Details:

• Gene ID:
• Gene Symbol: NaStEP
• Gene Name: Stigma Expressed Protein
• Genome:
• Species: Nicotiana alata

Functional Descriptions:

• Here the isolation and characterization of a stigma-specific protein from N. alata, NaStEP (N. alataStigma Expressed Protein), that is homologous to Kunitz-type proteinase inhibitors, are reported.
• Activity gel assays showed that NaStEP is not a functional serine proteinase inhibitor.
• NaStEP contains the vacuolar targeting sequence NPIVL, and immunocytochemistry experiments showed vacuolar localization in unpollinated stigmas.
• The transfer of NaStEP to the stigmatic exudate was accompanied by perforation of the stigmatic cell wall, which appeared to release the vacuolar contents to the apoplastic space.
• The increase in NaStEP synthesis after pollination and its presence in the stigmatic exudates suggest that this protein may play a role in the early pollen–stigma interactions that regulate pollen tube growth in Nicotiana.

Function-related keywords:

• growth ,  pollen ,  pollen-wall ,  cell-wall ,  cell-wall-synthesis ,  protein

Literature:

• Pollination in Nicotiana alata stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue.   DOI:  10.1093/jxb/ern175 ;  PMID:  18689443

Related News:

Gene Resources:

• NCBI ID: EU253563.1
• UniProt accessions: A9UF60

Orthologs:

Sequences:

• CDS Sequence:
• Protein Sequence: