Gene Details:

Functional Descriptions:

  • MtEFD and MtEFD2: Two transcription factors with distinct neofunctionalization in symbiotic nodule development.
  • A domain swap experiment showed that MtEFD2 differs from MtEFD by its C-terminal fraction outside the DNA binding domain. Furthermore, clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Cas9) mutagenesis of MtEFD2 led to a reduction in the number of nodules formed in Mtefd-1, with downregulation of a set of genes, including notably NUCLEAR FACTOR-YA1 (MtNF-YA1) and MtNF-YB16, which are essential for nodule meristem establishment.
  • Conclude that nitrogen-fixing symbiosis recruited two proteins originally expressed in roots, MtEFD and MtEFD2, with distinct functions and neofunctionalization processes for each of them.
  • MtEFD and MtEFD2, which exhibit distinct but overlapping expression profiles, could both be involved in the control of nodulation.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Orthologs:

Sequences:

  • CDS Sequence:
  • Protein Sequence: