Gene Details:

• Gene ID:
• Gene Symbol: MKS1
• Gene Name: Methylketone Synthase1
• Genome: Tomato Genome version SL4.0
• Species: Solanum lycopersicum

Functional Descriptions:

• We report crystal structures of S. habrochaites MKS1, an atypical member of the α/β-hydrolase superfamily. Sequence comparisons revealed the MKS1 catalytic triad, Ala-His-Asn, as divergent to the traditional α/β-hydrolase triad, Ser-His-Asp.
• Determination of the MKS1 structure points to a novel enzymatic mechanism dependent upon residues Thr-18 and His-243, confirmed by biochemical assays.
• We confirmed the importance of this substrate binding mode by substituting several amino acids leading to an alteration in the acyl-chain length preference of MKS1.
• We employ structure-guided mutagenesis and functional assays to demonstrate that MKS1, unlike enzymes from this hydrolase superfamily, is not an efficient hydrolase but instead catalyzes the decarboxylation of 3-keto acids.

Function-related keywords:

• hydrolase

Literature:

• Emergent decarboxylase activity and attenuation of α/β-hydrolase activity during the evolution of methylketone biosynthesis in tomato.   DOI:  10.1105/tpc.111.093997 ;  PMID:  22523203

Related News:

Gene Resources:

• NCBI ID: GU987105
• UniProt accessions:

Orthologs:

Sequences: