Gene Details:

  • Gene ID: Glyma.20G111000
  • Gene Symbol: GmFAD2–1B
  • Gene Name: Fatty Acid Desaturase 2
  • Genome:
  • Species: Glycine max

Functional Descriptions:

  • Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean.
  • The fatty acid profile analysis of T1 seeds derived from CRISPR-edited plants homozygous for both GmFAD2 genes showed dramatic increases in oleic acid content to over 80%, whereas linoleic acid decreased to 1.3–1.7%.
  • Palmitic and linolenic acid levels were also significantly reduced in the double GmFAD2 mutants, whereas stearic acid levels were comparable to wildtype.
  • Using the GmFAD2- 1A and GmFAD-1B genes are targets, we demonstrated that dual gRNA CRISPR/Cas9 system induced high frequency of heritable mutations in homeologous soybean genes.

Literature:

Gene Resources:

Orthologs:

Sequences:

  • CDS Sequence:
  • Protein Sequence: